• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.8
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• pp.1164-1168
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• 2005

Korean traditional deer decoction (Nogjungtang), composed of oriental herbs and almost deer parts, has been used as an important resource for human health. For basic studying on Nogjungtang, this experiment was conducted to evaluate nutritional effects of Nogjungtang, and estimate its effect on growth, food efficiency, organ development and hematological indices in growing and adult Sprague-Dawley rats. The rats were divided into three groups as follows; control: non-supplementation, Notiungtang I: recommended dose, and Nogjungtang II: thrice recommended dose. Nogjungtang was composed of various general nutrients with up 93$\%$ moisture.Crude protein is the highest value as 22.78$ \%$, Mg is a major mineral as 0.48$\%$ compared to other minerals, and methionine and proline are higher by 1.31 and 1.67$\%$ than other amino acids based on dry matter, re-spectively. In both growing and adult rats, there were no significant difference in body weight gain and feed intake between the control and Nogjungtang groups. Also, organs weights (liver, heart, kidney and stomach) and hematological indices (WBC, RBC, Hb, Hct and Platelet) did not show statistically significant differences among the experimental groups. However, all of experimental rats were normal growth without hypertrophy or negative development of organs by Nogjungtang. Hematological indices maintained in normal value by thrice recommended dose of Nogjungtang. The average body weight of each treatment groups showed similar levels at end of experiment. In case of the feed efficiency ratio (feed intake/body gain), the growing rats showed 6.00, 5.81 and 5.99 and adult rats showed 9.03, 8.98 and 9.10 in control, Nogjungtang I and Nogjungtang II, respectively. In conclusion, although further investigation of Nogjungtang should be performed in the functions registered in many ancient literatures, Nogjungtang is physiologically safe and may have potential as candidate food for human health.

• Journal of Korean Society of Forest Science
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• v.78 no.3
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• pp.280-286
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• 1989

The present study was to examine the effects of light, temperature, and water stress on the photosynthesis and respiration rates and its seasonal changes in 3-Year-old Fraxinus rhynchophylla and Fraxinus mandshurica seedlings raised in pot in nursery. The results obtained are as follows: 1. The estimated light compensation points at which net photosynthesis approached zero were 35 and $28{\mu}Em^{-2}\;s^{-1}$ for F. rhyrachophylla and F. mandsdzurica leaves, respectively, The light saturation points occurred at $700{\mu}Em^{-2}\;s^{-1}$ in these species. 2. The maximum rates of net photosynthesis of leaves occurred at about $25^{\circ}C$ in F. rhynchophylla and at $20^{\circ}C$ in F. mandshurica. 3. The decrease in net photosynthetic rates of leaves began at -13 bars in F. rhynchophylla and -10 bars in F. mandshurica, and then its rates approached approximately zero at -23 bars in F. mandshurica and -29 bars in F. rhynchophylla. 4. The dark respiration rates of leaves were similar from $10^{\circ}C$ to $40^{\circ}C$ between these species, Also these increased from -10 bars to -28 bars, and then decreased remarkably in these species. 5. The net photosynthetic rates of leaves of F. mandshurica were higher than those of F. rhynchoplaylla until June, and after that lower than F. rhynchophylla. The dark respiration rates of leaves of F. rhynchophylla were higher than those of F. mandshurica from June to August, and After that similar between these species. 6. The respiration rates of stem and root were highest in April during the growing season. These values of F. rhynchophylla were slightly higher than those of F. mandshurica throughout the growing season.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.35 no.1
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• pp.47-55
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• 2009

In this study, the antioxidative effects, inhibitory effects on tyrosinase, and component analysis of fermented Melissa officinalis extracts were investigated. The ethyl acetate fraction of fermented extract ($8.38{\mu}g/mL$) showed the most prominent the free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activities ($FSC_{50}$) of extract/fractions of M. officinalis. Reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) of some M. officinalis extracts on ROS generated in $Fe^{3+}$-EDTA/$H_{2}O_{2}$ system were investigated using the luminol-dependent chemiluminescence assay. The ethyl acetate fraction of fermented extract ($0.63{\mu}g/mL$) showed the most prominent ROS scavenging activity. The protective effects of extract/fractions of M. officinalis on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The M. officinalis extracts suppressed photohemolysis in a concentration dependent manner ($5\;{\sim}\;75{\mu}g/mL$). The inhibitory effect of M. officinalis extracts on tyrosinase was investigated to assess their whitening efficacy. Inhibitory effects ($IC_{50}$) on tyrosinase of some M. officinalis extracts was 50 % ethanol extract ($365{\mu}g/mL$) < ethyl acetate fraction of fermented extract ($122.43{\mu}g/mL$) < ethylacetate fraction ($94.8{\mu}g/mL$). Fractions of ethyl acetate both from ordinary and fermented M. officinalis extracts showed 2 band in TLC and 2 peak in HPLC (330 nm). In HPLC chromatogram of ethyl acetate fraction, peak 1 (51.64 %) and peak 2 (48.36 %) were identified as caffeic acid and rosmarinic acid in the order of elution time. Also, in HPLC chromatogram of ethyl acetate fraction of fermented extract, peak 1 (4.13 %) and peak 2 (95.87 %) were identified as caffeic acid and rosmarinic acid in the order of elution time. These results indicate that the component and content of ordinary and fermented extracts of M. officinalis are different. And the extract of M. officinalis can be used as an antioxidant.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.39 no.4
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• pp.259-269
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• 2013

In this work, licorice extracts were prepared using various extraction conditions such as extraction solvent, temperature, and time from Glycyrrhiza uralensis (G. uralensis) produced in Korea and China and Glycyrrhiza glabra (G. glabra) in Uzbekistan. The optimum extraction condition was selected from the extraction yields and antioxidative activities of extracts. Korea licorice extracts showed the highest free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity (46.05%) under the extraction condition of 85% ethanol at $60^{\circ}C$ for 6 hours. The prominent ROS (reactive oxygen species) scavenging activity using luminol-dependent chemiluminescence assay and the cellular protective effect against $^1O_2$ induced cellular membrane damage were also shown from the extracts obtained from the same condition. Especially, Korea G. uralensis extracts exhibited the higher prominent protective effect (${\tau}_{50}$ = 116.4 min) than (+)-(+)-${\alpha}$-tocopherol (${\tau}_{50}$ = 28.5 min) and the extraction yield of Korea licorice extract was 18.75%, which is 1.2 times and 2.5 times higher than that of Uzbekistan and China, respectively. These results indicate that the extraction condition of 85% ethanol at $60^{\circ}C$ for 6 hours is optimal to prepare licorice extracts, which can be applicable as anti-oxidative cosmetic materials.

• Korean Journal of Plant Resources
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• v.30 no.1
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• pp.38-49
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• 2017

The objective of this research was to develop Near-Infrared Reflectance Spectroscopy (NIRS) model for amylose and protein contents analysis of large accessions of rice germplasm. A total of 511 accessions of rice germplasm were obtained from National Agrobiodiversity Center to make calibration equation. The accessions were measured by NIRS for both brown and milled brown rice which was additionally assayed by iodine and Kjeldahl method for amylose and crude protein contents. The range of amylose and protein content in milled brown rice were 6.15-32.25% and 4.72-14.81%, respectively. The correlation coefficient ($R^2$), standard error of calibration (SEC) and slope of brown rice were 0.906, 1.741, 0.995 in amylose and 0.941, 0.276, 1.011 in protein, respectively, whereas $R^2$, SEC and slope of milled brown rice values were 0.956, 1.159, 1.001 in amylose and 0.982, 0.164, 1.003 in protein, respectively. Validation results of this NIRS equation showed a high coefficient determination in prediction for amylose (0.962) and protein (0.986), and also low standard error in prediction (SEP) for amylose (2.349) and protein (0.415). These results suggest that NIRS equation model should be practically applied for determination of amylose and crude protein contents in large accessions of rice germplasm.

• Korean journal of applied entomology
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• v.13 no.1 s.18
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• pp.1-10
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• 1974

The activities of pectolytic and cellulolytic enzymes produced from slices of ginseng root infected with Cylindrocarpon destructains(Zins.) Scholtern were proportional to each concentration and reaction time. Activities of cellulase(Cx), endo-polygalacturonase(endo-PG), endo-polymethylg-alacturonase(endo-PMG), exo-polygalacturonase(exe-PG), and exe-polymethylgalacturonase(exo-PMG) were maximum on the 4th day after inoculation. No endo-PG and endo-PMG were detected at the first and second days, while exo-PG exo-PMG were active. On the 6th day, all pectic enzymes were completely lost, whereas Cx remained at a high concentration. pH optima of Cx, endo-PG, endo-PMG, exo-PG, and exo-PMG were 6.0, 5.5, 8.0, 7.0 to 7.5, and 8.5, respectively. Temperature optima of Cx, endo-PG, endo-PMG exo-PG, and exo-PMG were $66^{\circ}C\;53^{\circ}C\;41^{\circ}C\;37^{\circ}C\;and\;40^{\circ}C$, respectively. Cx was only inhibited by $0.05M\; Hg^{++}$ among 16 ions tested. Inhibitory effects of ions on pectolytic enzymes varied, however$M Fe^{+++}\;and\;0.05M\;Al^{+++}$ were the best in general. Among 8 fungicides, none of them inhibited all the enzymes studied at $0.1\%$, active ingredients. Exo-PG were highly inhibited by all of the fungicides, of which difolatan was the most inhibitory to all the pectic enzymes. $Ca^{++}\; at\; 0.02M\; and\;Fe^{+++}\;at\;0.02M$ completely inhibited all the pectolytic enzymes, and Cx was inhibited $30\%$ and $70\%$ at the same concentration, respectively Formalin almost inhibited exo-PG and exe-PMG at $0.8\%$ but not the other enzymes especially Cx. Difolatan at $0.8\%$ inhibited all the enzymes concerned above $80\%$. The cellulolytic and pectolytic enzymes of C. destructans must be closely associated with the ginseng root rot and should be inhibited to control the disease effectively.

• Korean journal of applied entomology
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• v.55 no.4
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• pp.453-457
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• 2016

This study investigates the hatching periods and hatchability of the eggs of Pochazia shantungensis at different collection times from 2011 to 2014, and the effect of temperature on the growth of P. shantungensis nymphs in an area of its outbreak. The hatchability of P. shantungensis eggs varies with their collection time; their hatchability in late November was higher than that in March of the next year, but no difference was observed in their hatching periods. The hatching periods of the eggs were 51.2, 31.3, 24.8, 19.4, 17.1, and 19.4 days at 15, 18, 21, 24, 27, and $30^{\circ}C$, respectively. The hatchability was above 70% at temperatures ranging from 18 to $27^{\circ}C$. The hatching time of the overwintered eggs in the Gurye region in Korea was reduced by 9 days from 2011 to 2014. The hatching rate was relatively higher when the average temperature in the winter season was relatively warmer. The dvelopmental periods of the first to fifth nymphs were 82.8, 58.0, 45.8, and 39.6 days at 18, 21, 24, and $27^{\circ}C$, respectively, at the relative humidity of 40~70%, and a photoperiod off 14 h light:10 h dark. The higher the temperature, the shorter the developmental period. At $30^{\circ}C$, all life stages after the fourth nymph died. Thus, the optimum growth temperature was estimated to be $27^{\circ}C$. For all life stages from the egg to the fourth nymph, the relationship between the temperature and developmental rate was expressed by the linear equation Y = 0.0015 X - 0.014. The lower developmental threshold was $9.3^{\circ}C$ and the effective cumulative temperature was 693.3 degree-days. The lower developmental threshold of approximately $3.8^{\circ}C$ was the lowest at the fourth nymph stage.

• Korean Journal of Environment and Ecology
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• v.27 no.5
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• pp.592-613
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• 2013

This study was carried out to analyze the vegetation properties, soil characteristic, and ordination of forest in Hongdo island and the results are as follows: Castanopsis cuspidata var. sieboldii community, Quercus acuta community, Machilus thunbergii community, Pinus densiflora community and Carpinus coreana community. The results of importance value on the vegetation of Hongdo island consisted of the following in order: 75.56% of Castanopsis cuspidata var. sieboldii, the highest, 34.84% of Pinus densiflora, 29.11% of Machilus thunbergii, 26.88% of Camellia japonica, 17.79% of Quercus acuta, 15.23% of Dendropanax morbifera, 13.41% of Ligustrum japonicum, 12.67% of Carpinus coreana. Due to the high organic matter, total nitrogen and low pH, the soil properties of Hongdo island are different from those of other forest in Korea. The composition of soil properties in the same areas are as follows: organic matter, total nitrogen, available phosphorous, exchangeable $K^+$, exchangeable $Ca^{2+}$, exchangeable $Mg^{2+}$ contained, and soil pH. The capacities of these chemical properties of the soil ranged from 14.48~25.45%, 0.49~0.90%, 26.00~58.68 mg/kg, $0.64{\sim}1.64cmol^+/kg$, $6.22{\sim}21.5cmol^+/kg$, $2.92{\sim}6.95cmol^+/kg$, and 4.42~5.44 respectively. The results of the correlation between communities and soil conditions of vegetation of Hongdo island by DCCA ordination method are as follows: Castanopsis cuspidata var. sieboldii community was found in the highest soil moisture and followed in order Machilus thunbergii community, Quercus acuta community. The Machilus thunbergii community was found in the highest pH, exchangeable $Ca^{2+}$, exchangeable $Mg^{2+}$, exchangeable $K^+$ and cation exchange capacity. The Carpinus coreana community was found in the highest elevation, total nitrogen, cation exchange capacity, exchangeable $Mg^{2+}$ and followed in order Pinus densiflora community.

• The korean journal of orthodontics
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• v.36 no.6
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• pp.422-433
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• 2006

Objective: Several ions and components are released from self-etching primers in the oral cavity. This may cause injury to the periodontal tissues throughout orthodontic treatment. The purpose of this study was to assess the cytotoxicity of self-etching primers to HGF-1, HaCaT, and RHEK cells. Method: Transbond XT Primer (3M Unitek, Monrovia, CA, USA), and self-etching primers, Clearfil SE Bond (Kuraray, Osaka, Japan), Transbond Plus SEP (3M Unitek, Monrovia, CA, USA), and Adper Prompt L-Pop (3M Unitek, Monrovia, CA, USA), were evaluated by MTT assay, and cellular changes were also observed. Results: In all cells after 72 hours with all primers, severe morphological changes such as atrophy and necrosis were observed. In the MTT assay using HGF-1, Clearfil SE Bond, Transbond XT Primer, Transbond Plus SEP, and Adper Prompt L-Pop were lined up in order of ascending cytotoxicity When using HaCaT, Clearfil SE Bond, Adper Prompt L-Pop, Transbond Plus SEP, and Transbond XT Primer were lined up in order of ascending cytotoxicity. When using RHEK, Clearfil SE Bond, Transbond XT Primer, Adper Prompt L-Pop, and Transbond Plus SEP were lined up in order of ascending cytotoxicity. Conclusion: The result of this study shows that care is needed because self-etching primers show cytotoxic properties similar to conventional primers.

• Journal of Oral Medicine and Pain
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• v.30 no.2
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• pp.215-230
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• 2005

The study was intended to investigate how dentists in private dental clinic thought on the present claim and review of dental insurance to reflect it in future establishing dental insurance policies. 1,465 dentists who were running own dental clinic in Pusan Metropolitan City and the south part of Kyungsang province were surveyed in February, 2004. A total of 406 copies of finished questionnaire were finally retrieved and analyzed. The findings are as follows. 1. About insurance claim affairs : Most of the subject of insurance claim was by dentist himself or dental hygienist(nurse). Agency claiming was carried under 20% of total insurance claim. 2. The degree of attendance on insurance lecture : The degree of attendance on insurance lecture was relatively low. 3. Filing a protest against insurance claim : Filing a protest against insurance claim was reavealed about half-and-half for "have been" or "have not been". 4. Private clinic dentist,s opinion about the regulations affecting review of dental insurance : Private clinic dentists opinion about current guide for insurance review of dental fee was“the guidance is difficult and unfair cutback of claim fee may be carried”. 5. The affairs about health insurance review agency : About 70% of private clinic dentists have dissatisfaction on health insurance review agency. 6. Standpoint of private clinic dentists about issuance of receipt for dental fee : About 70% of private clinic dentist have an difficulty in issuance of receipt for dental fee. 7. The affairs about change insurance noncoverage treatment to insurance coverage treatment : Most of private clinic dentists hoped that insurance coverage about full mouth scaling, pit and fissure sealant, fluoride application. But they do not hoped that insurance coverage about geriatric denture, prothodontic treatment except precious metal, photopolymerization resin treatment.