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In vitro cytotoxicity of self-etching primers  

Rhee, Chang-Hoon (Department of Orthodontics, School of Dentistry, Pusan National University)
Kim, In-Ryeon (Department of Oral Anatomy, School of Dentistry. Research Institute for Oral Biotechnology, Pusan National University)
Kim, Gyoo-Cheon (Department of Oral Anatomy, School of Dentistry. Research Institute for Oral Biotechnology, Pusan National University)
Kim, Seong-Sik (Department of Orthodontics, School of Dentistry. Research Institute for Oral Biotechnology, Pusan National University)
Son, Woo-Sung (Department of Orthodontics, School of Dentistry. Research Institute for Oral Biotechnology, Pusan National University)
Publication Information
The korean journal of orthodontics / v.36, no.6, 2006 , pp. 422-433 More about this Journal
Abstract
Objective: Several ions and components are released from self-etching primers in the oral cavity. This may cause injury to the periodontal tissues throughout orthodontic treatment. The purpose of this study was to assess the cytotoxicity of self-etching primers to HGF-1, HaCaT, and RHEK cells. Method: Transbond XT Primer (3M Unitek, Monrovia, CA, USA), and self-etching primers, Clearfil SE Bond (Kuraray, Osaka, Japan), Transbond Plus SEP (3M Unitek, Monrovia, CA, USA), and Adper Prompt L-Pop (3M Unitek, Monrovia, CA, USA), were evaluated by MTT assay, and cellular changes were also observed. Results: In all cells after 72 hours with all primers, severe morphological changes such as atrophy and necrosis were observed. In the MTT assay using HGF-1, Clearfil SE Bond, Transbond XT Primer, Transbond Plus SEP, and Adper Prompt L-Pop were lined up in order of ascending cytotoxicity When using HaCaT, Clearfil SE Bond, Adper Prompt L-Pop, Transbond Plus SEP, and Transbond XT Primer were lined up in order of ascending cytotoxicity. When using RHEK, Clearfil SE Bond, Transbond XT Primer, Adper Prompt L-Pop, and Transbond Plus SEP were lined up in order of ascending cytotoxicity. Conclusion: The result of this study shows that care is needed because self-etching primers show cytotoxic properties similar to conventional primers.
Keywords
Cytotoxicity; Self-etching primer;
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