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http://dx.doi.org/10.15230/SCSK.2013.39.4.259

Screening of Effective Extraction Conditions for Increasing Antioxidant Activities of Licorice Extracts from Various Countries of Origin  

Ha, Ji Hoon (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology)
Lee, Hye Mi (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology)
Kwon, Soon Sik (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology)
Kim, Hae Soo (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology)
Kim, Moon Jin (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology)
Jeon, So Ha (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology)
Jeong, Yoo Min (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology)
Hwang, Jun Pil (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology)
Park, Jong-Ho (DAEBONG LS, Ltd.)
Choi, Yung-Key (DAEBONG LS, Ltd.)
Park, Jino (DAEBONG LS, Ltd.)
Park, Soo Nam (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology)
Park, Dong-Sik (Functional Food & Nutrition Division, Department of Agrofood Resources)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.39, no.4, 2013 , pp. 259-269 More about this Journal
Abstract
In this work, licorice extracts were prepared using various extraction conditions such as extraction solvent, temperature, and time from Glycyrrhiza uralensis (G. uralensis) produced in Korea and China and Glycyrrhiza glabra (G. glabra) in Uzbekistan. The optimum extraction condition was selected from the extraction yields and antioxidative activities of extracts. Korea licorice extracts showed the highest free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity (46.05%) under the extraction condition of 85% ethanol at $60^{\circ}C$ for 6 hours. The prominent ROS (reactive oxygen species) scavenging activity using luminol-dependent chemiluminescence assay and the cellular protective effect against $^1O_2$ induced cellular membrane damage were also shown from the extracts obtained from the same condition. Especially, Korea G. uralensis extracts exhibited the higher prominent protective effect (${\tau}_{50}$ = 116.4 min) than (+)-(+)-${\alpha}$-tocopherol (${\tau}_{50}$ = 28.5 min) and the extraction yield of Korea licorice extract was 18.75%, which is 1.2 times and 2.5 times higher than that of Uzbekistan and China, respectively. These results indicate that the extraction condition of 85% ethanol at $60^{\circ}C$ for 6 hours is optimal to prepare licorice extracts, which can be applicable as anti-oxidative cosmetic materials.
Keywords
Glycyrrhiza uralensis; Glycyrrhiza glabra; DPPH assay; luminol chemiluminescence assay; cellular protective effect;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
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