Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
권호 표지
DOI QR코드

DOI QR Code

Screening of Effective Extraction Conditions for Increasing Antioxidant Activities of Licorice Extracts from Various Countries of Origin

원산지별 감초추출물의 항산화활성 증가를 위한 효율적인 추출조건 탐색

  • Ha, Ji Hoon (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology) ;
  • Lee, Hye Mi (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology) ;
  • Kwon, Soon Sik (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology) ;
  • Kim, Hae Soo (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology) ;
  • Kim, Moon Jin (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology) ;
  • Jeon, So Ha (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology) ;
  • Jeong, Yoo Min (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology) ;
  • Hwang, Jun Pil (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology) ;
  • Park, Jong-Ho (DAEBONG LS, Ltd.) ;
  • Choi, Yung-Key (DAEBONG LS, Ltd.) ;
  • Park, Jino (DAEBONG LS, Ltd.) ;
  • Park, Soo Nam (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology) ;
  • Park, Dong-Sik (Functional Food & Nutrition Division, Department of Agrofood Resources)
  • 하지훈 (서울과학기술대학교 정밀화학과 화장품종합기술연구소) ;
  • 이혜미 (서울과학기술대학교 정밀화학과 화장품종합기술연구소) ;
  • 권순식 (서울과학기술대학교 정밀화학과 화장품종합기술연구소) ;
  • 김해수 (서울과학기술대학교 정밀화학과 화장품종합기술연구소) ;
  • 김문진 (서울과학기술대학교 정밀화학과 화장품종합기술연구소) ;
  • 전소하 (서울과학기술대학교 정밀화학과 화장품종합기술연구소) ;
  • 정유민 (서울과학기술대학교 정밀화학과 화장품종합기술연구소) ;
  • 황준필 (서울과학기술대학교 정밀화학과 화장품종합기술연구소) ;
  • 박종호 ((주)대봉엘에스) ;
  • 최영기 ((주)대봉엘에스) ;
  • 박진오 ((주)대봉엘에스) ;
  • 박수남 (서울과학기술대학교 정밀화학과 화장품종합기술연구소) ;
  • 박동식 (농촌진흥청 국립농업과학원 농식품자원부 기능성식품과)
  • Received : 2013.07.01
  • Accepted : 2013.07.10
  • Published : 2013.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

In this work, licorice extracts were prepared using various extraction conditions such as extraction solvent, temperature, and time from Glycyrrhiza uralensis (G. uralensis) produced in Korea and China and Glycyrrhiza glabra (G. glabra) in Uzbekistan. The optimum extraction condition was selected from the extraction yields and antioxidative activities of extracts. Korea licorice extracts showed the highest free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity (46.05%) under the extraction condition of 85% ethanol at $60^{\circ}C$ for 6 hours. The prominent ROS (reactive oxygen species) scavenging activity using luminol-dependent chemiluminescence assay and the cellular protective effect against $^1O_2$ induced cellular membrane damage were also shown from the extracts obtained from the same condition. Especially, Korea G. uralensis extracts exhibited the higher prominent protective effect (${\tau}_{50}$ = 116.4 min) than (+)-(+)-${\alpha}$-tocopherol (${\tau}_{50}$ = 28.5 min) and the extraction yield of Korea licorice extract was 18.75%, which is 1.2 times and 2.5 times higher than that of Uzbekistan and China, respectively. These results indicate that the extraction condition of 85% ethanol at $60^{\circ}C$ for 6 hours is optimal to prepare licorice extracts, which can be applicable as anti-oxidative cosmetic materials.

본 연구에서는 한국 및 중국산 감초(Glycyrrhiza uralensis), 그리고 우즈베키스탄산 감초(Glycyrrhiza glabra)를 대상으로 추출용매, 추출온도, 추출시간 등 추출조건별 추출물을 제조하고 이들 추출물들의 추출 수율과 항산화 활성을 비교하여 최적의 추출조건을 선정하였다. 항산화 활성 중 자유 라디칼(1,1-phenyl-2-picrylhydrazyl, DPPH) 소거활성은 85% 에탄올로 $60^{\circ}C$에서 6 h 동안 추출한 조건에서 한국 감초로부터 얻은 추출물이 가장 높은 활성(46.05%)을 나타내었다. 루미놀 발광법을 이용한 추출물들의 총 항산화능 측정 실험과 피부 광노화에 중요한 $^1O_2$으로 유도된 세포막 손상에 대한 추출물들의 세포 보호 효과를 측정한 실험 모두에서도 위의 조건에서 가장 높은 항산화 활성을 나타내었다. 특히, 한국 감초는 ${\tau}_{50}$이 116.4 min으로 비교 물질인 (+)-${\alpha}$-tocopherol (28.5 min)보다 약 4 배나 높은 세포 보호 효과를 나타내었으며, 추출 수율은 18.75%로 우즈베키스탄 및 중국 감초보다 각각 1.2 배 및 2.5 배의 추출 수율을 나타내었다. 따라서, 본 연구 결과는 항산화 소재로 화장품에 응용하기 위하여 감초로부터 추출물을 얻기 위한 최적의 조건은 85% 에탄올로 $60^{\circ}C$에서 6시간 동안 추출하는 것임을 보여주었다.

Keywords

References

  1. S. N. Park, Skin aging and antioxidant, J. Soc. Cosmet. Scientists Korea, 23(1), 75 (1997).
  2. S. N. Park, Antioxidative properties of baicalein, component from Scutellaria baicalensis Georgi and its application to cosmetics(I), J. Korean Int. Eng. Chem., 14(5), 657 (2003).
  3. S. N. Park, Protective effect of isoflavone, genistein from soybean on singlet oxygen induced photohemolysis of human erythrocytes, Korean J. Food Sci. Technol., 35(3), 510 (2003).
  4. S. N. Park, Effects of flavonoids and other phenolic compounds on reactive oxygen-mediated biochemical reactions, Ph. D. Dissertation, Seoul National Univ., Seoul, Korea (1989).
  5. K. Scharffetter-Kochanek, Photoaging of the connetive tissue of skin: its prevention and therapy, In: Antioxidants in disease mechanisms and therapy, Advances pharmacology, (H. Sies, eds), 639 (1997).
  6. S. N. Park, S. Y. Kim, G. N. Lim, N. R. Jo, and M. H. Lee, In vitro skin permeation and cellular protective effects of flavonoids isoplated from Suaeda asparagoides extracts, J. Ind. Eng. Chem., 18(2), 680 (2012). https://doi.org/10.1016/j.jiec.2011.11.126
  7. N. R. Jo, H. A. Gu, S. A. Park, S. B. Han, and S. N. Park, Cellular protective effect and liposome formulation for enhanced transdermal delivery of isoquercitrin, J. Soc. Cosmet. Scientists Korea, 38(2), 103 (2012). https://doi.org/10.15230/SCSK.2012.38.2.103
  8. T. S. Yoon, M. S. Cheon, S. J. Kim, A. Y. Lee, B. C. Moon, J. M. Chun, B. K. Choo, and H. K. Kim, Evaluation of solvent extraction on the anti-inflammatory efficacy, Korean J. Medicinal Crop Sci., 18(1), 28 (2010).
  9. E. Y. Ahn, D. H. Shin, N. I. Back, and J. A. Oh, Isolation and identification of antimicrobial active substance from G. uralensis, Korea J. Food Sci. Technol., 30(3), 680 (1998).
  10. J. H. Park, Q. Wu, K. H. Yoo, H. I. Yong, S. M. Cho, I. S. Chung, and N. I. Back, Cytotoxic effect of flavonoids from the roots of G. uralensis on human cancer cell lines, J. Appl. Biol. Chem., 54(1), 67 (2011). https://doi.org/10.3839/jabc.2011.012
  11. K. Kenji, S. Mao, N. Rie, M. Takashi, and S. Yukihiri, Constituent properties of licorice derived from G. uralensis, G. glabra, and G. inflata identified by genetic information, Biol. Pharm. Bull., 30, 1271 (2007). https://doi.org/10.1248/bpb.30.1271
  12. S. J. Kim, D. H. Kwoen, and J. H. Lee, Physiological activity/nutrition : Investigation of antioxidative activity and stability of ethanol extracts of licorice root (G. glabra), Korean J. Food Sci. Technol., 38, 584 (2006).
  13. H. K. Shim, M. H. Park, C. Choi, and M. J. Hae, Effect of G. glabra extracts on immune response, Korean J. Food Nutr., 10, 533 (1997).
  14. J. He, L. Chen, D. Heber, W. Shi, and Q. Lu, Antibacterial compounds from G. uralensis, J. Nat. Prod., 69, 121 (2006). https://doi.org/10.1021/np058069d
  15. S. B. Han, H. A. Gu, S. J. Kim, S. S. Kwon, H. S. Kim, S. H. Jeon, J. P. Hwang, and S. N. Park, Comparative study on antioxidative activity of G. uralensis and G. glabra extracts by country of origin, J. Soc. Cosmet. Scientists Korea, 39(1), 1 (2013). https://doi.org/10.15230/SCSK.2013.39.1.001
  16. H. J. Kim, J. Y. Bae, H. N. Jang, and S. N. Park, Comparative study on the antimicrobial activity of G. uralensis and G. glabra extracts with various countries of origin as natural antiseptics, Korean J. Microbiol. Biotechnol., 41(3), 358 (2013). https://doi.org/10.4014/kjmb.1307.07003
  17. H. J. Kim, H. N. Jang, J. Y. Bae, and S. N. Park, A study on the stability of the cream containing G. uralensis extract, J. Soc. Cosmet. Scientists Korea, 39(2), 117 (2013). https://doi.org/10.15230/SCSK.2013.39.2.117
  18. S. L. Shin and C. H. Lee, Screening of effective extraction conditions for increasing antioxidant activities from fronds of Osmunda japonica, Korean J. Plant Res., 24(2), 174 (2011). https://doi.org/10.7732/kjpr.2011.24.2.174
  19. M. M. Cowan, Plant products as antimicrobial agents, Clin. Microbiol. Rev., 12, 564 (1999).
  20. J. H. Woo, S. L. Shin, Y. D. Chang, and C. H. Lee, Comparison of antioxidant effects by different extraction methods in flowers of Aster scaber, Aster maackii, Coreopsis lanceolata and Coreopsis tinctoria, Kor. J. Plant. Res., 22, 381 (2009).

Cited by

  1. Antioxidant and Antibacterial Activities of Glycyrrhiza uralensis Fisher (Jecheon, Korea) Extracts Obtained by various Extract Conditions vol.41, pp.4, 2015, https://doi.org/10.15230/SCSK.2015.41.4.361