• Korean Journal of Breeding Science
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• v.43 no.5
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• pp.448-456
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• 2011

We used suppression subtractive hybridization (SSH) combined with mirror orientation selection (MOS) method to screen differentially expressed genes from red-fleshed kiwifruit 'Hongyang'. As a result, the 288 clones were obtained by subcloning PCR product and 192 clones that showed positive clones on colony PCR analysis were selected. All the positive clones were sequenced. After comparisons with the NCBI/Genbank database using the BLAST search revealed that 30 clones showed sequence similarity to genes from other organisms; 10 clones showed significant sequence similarity to known genes. Among these clones, 3 clones (AcF21, AcF42 and AcF106) had sequence homology to 1-aminicyclopropane-carboxylic acid (ACC)-oxidase (ACO) that known to be related to fruit ripening. The expression patterns of differentially expressed genes were further investigated to validate the SSH data by reverse transcription PCR (RT-PCR) and quantitative real-time PCR (qReal-time PCR) analysis. All the data from qReal-time PCR analysis coincide with the results obtained from RT-PCR analysis. Three clones were expressed at higher levels in 'Hongyang' than 'Hayward'. AcF21 was highly expressed in the other genes at 120 days after full bloom (DAFB) and 160 DAFB of 'Hongyang'.

• Korean Journal of Breeding Science
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• v.42 no.5
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• pp.525-533
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• 2010

In this study, random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) analyses were utilized for evaluation of genetic diversity of 34 Korean bred and introduced apple cultivars. Thirty-seven RAPD primers detected a total of 193 polymorphic bands (36.2%) with an average of 5.6. Twenty-six SSR markers generated a total of 112 alleles with an average 4.3 alleles per locus. Genetic diversity of 34 cultivars estimated by polymorphic information content (PIC) value ranged from 0.536 (CH03d12) to 0.952 (CH04c06) with an average of 0.843. By UPGMA (unweighted pair-group method arithmetic average) cluster analysis with 305 polymorphic bands, the apple cultivars were classified four groups by similarity index of 0.640. The 'Seokwang' was included in group I. Group II consisted of 12 cultivars which have 'Golden Delicious' in their pedigree, with the exception of 'Spur Earliblaze' and 'Jonathan'. Group III included 13 cultivars which have usually 'Fuji' in their ancestry and bud sport of 'Fuji' cultivars. Group IV consisted of 8 cultivars with 'Hongro', 'Gamhong', and 'Saenara'. Similarity values among the tested apple cultivars ranged from 0.529 to 0.987, and the average similarity value was 0.647. The similarity index was the highest (0.987) between 'Hwarang' and 'Danhong', and the lowest (0.529) between 'Seokwang' and 'Hwarang'. The genetic relationships among the 34 studied apple cultivars were basically consistent with the known pedigree.

• Journal of Life Science
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• v.15 no.1 s.68
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• pp.132-135
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• 2005

This experiment was performed to obtain the basic information on heterosis and heterobeltiosis of quantitative characters in the eggplant by crossing of varieties. The heterosis and heterobeltiosis were estimated in Fl generation. The results were as follows, based on the graphical analysis, the characters, plant height, stem diameter, fruit weight, leaf length, leaf width, and fruit number per plant(yield) were assumed to inherit dominance, and fruit length and fruit diameter were found to inherit incomplete dominance. The degree of positive heterosis of plant height, stem diameter, fruit weight, fruit length, fruit diameter, leaf length, leaf width, and fruit diameter, leaf width, leaf length, fruit weight, and stem diameter showed negative heterobeltiosis.

• Korean Journal of Breeding Science
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• v.42 no.5
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• pp.495-501
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• 2010

Peaches (Prunus persica) are less popular than the fresh fruits, because their flesh gets soft faster. So many breeders focused on their aim to firmness. Other breeders focused on juiciness, flavor and aroma. Breeding requires much labor, time and money. To reduce these requirements, many scientists develop many SSR, CAPS and SCAR makers. New peach varieties bred in our National Institute of Horticultural & Herbal Science (NIHHS) such as, Cheonhong, Suhong and Harhong are yellow flesh cultivars and Yumyeong, Baekmijosaeng, Baekhyang, Jinmi, Soomee, Mihong, Misshong and Yumee are white flesh cultivars. These peach cultivars are planted in orchard of Korea. To assert breeding cultivar patents and prevent patent disputes, we detected cultivar-specific DNA fragment using 235 sets of Operon RAPD primers, analyzed 134 DNA sequences and constructed SCAR primers. To confirm the cultivar-specific SCAR markers, we applied candidate SCAR primers to 30 peach cultivars widely cultivated in Korea. These selected lines are included father and mother lines that were used to develop new varieties in NIHHS. Using fourteen SCAR primer sets, we characterized thirty cultivars selected. The SCAR marker is expected to serve as molecular evidence distinguishing different peach varieties.

• Journal of Plant Biotechnology
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• v.37 no.4
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• pp.408-413
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• 2010

The paper reviewed research status and outlook of genetic transformation in fruit trees in Korea. Genetic transformation has been long considered as an alternative technique overcoming limitation of conventional breeding and conducted since early 1990's. An efficient genetic transformation was established with major cultivars of apple, even in Fuji and Gamhong, and some transgenic apple plants have been transferred into the greenhouse for further analysis of gene expression. A few successes of genetic transformation have been reported and application to a variety of cultivars tried in citrus and kiwifruit. Successful genetic transformation has not been reported in the other fruit trees including grapevine, yet and it is considered being at the level of research. Those factors including replacement of antibiotics as a selective agent, use of transgenic rootstocks and manipulation of gene expression at proper parts and developmental stages have been prerequisites for the rapid commercialization of transgenic fruit plants.

• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.298-311
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• 2015

Grape is one of the important fruit crops around the world, and exposed to disease and pests, and internal or environmental stresses in the vineyards. Breeding and cultivation of new varieties of high quality-grapes resistant to diseases and pests and tolerant to stresses are the most important steps in the grape production. However, conventional breeding has laborious and time-consuming procedures in maintaining and selecting seedlings in the fields. Development of molecular breeding technology through understanding of molecular mechanism of useful traits can be used as an alternative strategy to improve the efficiency of grape breeding program by cross hybridization in grape development programs. The completion of the grape genome sequencing project provided the way to discover the novel genes and to analyze their functions. Comparative genomics, transcriptomic analysis, and the genome-wide identification and analysis of useful genes as well as development of molecular marker for valuable traits could provide novel insights into fruit quality and the responses to diseases and stresses, and can be used as important information in molecular breeding programs for grape development.

• Horticultural Science & Technology
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• v.17 no.1
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• pp.15-16
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• 1999

'Cheonhong'was released by the National Horticultural Research Institute in 1992 as a new mid-ripening, yellow-fleshed, and freestone nectarine variety, which originated from open-pollinated seeds of 'Garden State' nectarine collected in 1978. This was tested as 'Wonkyo Da-03' at 4 areas for 4 years from 1989. 'Cheonhong' is resistant to fruit cracking during rainy season and russeting. Fruits mature about 15 days before 'SunGlo' and have rich-flavor and good quality.

• Korean Journal of Breeding Science
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• v.51 no.4
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• pp.424-427
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• 2019

A persimmon (Diospyros kaki L. Thunb.) cultivar, 'Chosi', which was a cross between 'Johongsi' and 'Nishimurawase' in 2000, was finally selected in 2010. 'Chosi' persimmon fruits were harvested in Sept. 19 in Naju, with a medium fruit size of 161 g, on average, and high soluble solids of approximately 15.3 °Bx. The flesh tastes good, with a crispy texture, and the juice is rich. The shape of the fruit is round oblate, and the skin color is orange; the appearance is graceful. Physiological disorders, such as stylar-end cracking or skin fine cracking, rarely occur in this cultivar. (Registration No. 7075)

• Horticultural Science & Technology
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• v.17 no.1
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• pp.19-20
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• 1999

'Jinmi' was released by National Horticultural Research Institute in 1998 as a white-fleshed, mid- to late-maturing, and clingstone peach variety, which originated from the crossing of 'Hakuho' and 'Nunomewase' in 1982. The selection was tested for regional adaptability as 'Wonkyo Da-09' at 5 areas for 4 years from 1995. Fruits mature 5 to 7 days before 'Yumyeong' and have high sugar content, above $13.0^oBx$.

• Horticultural Science & Technology
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• v.17 no.1
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• pp.17-18
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• 1999

'Baekhyang' was released by National Horticultural Research Institute in 1994 as a white-fleshed, late-maturing, and freestone peach variety, which originated from open-pollinated seeds of 'Garden State' nectarine collected in 1978. This variety was tested as 'Wonkyo Da-05' at 4 areas for 4 years from 1991. 'Baekhyang' is the latest maturing among white-fleshed peach varieties in Korea. Fruits mature 10 to 15 days after 'Yumyeong' and have rich-flavor and good quality.