Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
권호 표지

Identification of new Breeding Lines by Prunus Persica Cultivar-Specific SCAR Primers

SCAR 마커 개발 및 이를 활용한 국내 육성 복숭아 품종 판별

  • Han, Sang Eun (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Cho, Kang-Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Nam, Eun Young (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Shin, Il-Sheob (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Chung Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Hyun Ran (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Dae-Hyun (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
  • Received : 2010.11.08
  • Published : 2010.12.31
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Abstract

Peaches (Prunus persica) are less popular than the fresh fruits, because their flesh gets soft faster. So many breeders focused on their aim to firmness. Other breeders focused on juiciness, flavor and aroma. Breeding requires much labor, time and money. To reduce these requirements, many scientists develop many SSR, CAPS and SCAR makers. New peach varieties bred in our National Institute of Horticultural & Herbal Science (NIHHS) such as, Cheonhong, Suhong and Harhong are yellow flesh cultivars and Yumyeong, Baekmijosaeng, Baekhyang, Jinmi, Soomee, Mihong, Misshong and Yumee are white flesh cultivars. These peach cultivars are planted in orchard of Korea. To assert breeding cultivar patents and prevent patent disputes, we detected cultivar-specific DNA fragment using 235 sets of Operon RAPD primers, analyzed 134 DNA sequences and constructed SCAR primers. To confirm the cultivar-specific SCAR markers, we applied candidate SCAR primers to 30 peach cultivars widely cultivated in Korea. These selected lines are included father and mother lines that were used to develop new varieties in NIHHS. Using fourteen SCAR primer sets, we characterized thirty cultivars selected. The SCAR marker is expected to serve as molecular evidence distinguishing different peach varieties.

복숭아의 기원은 중국으로 알려져 있으며, 저장기간이 짧아 소비자들의 선호도가 낮았다. 이런 이유로 육종가들은 저장성을 높이는 것과 향과 맛을 좋게 하는데 육종 목표를 설정했으며, 국립원예특작과학원 육종가들은 이 목표에 따라 새로운 품종('천홍', '수홍', '하홍', '유명', '백미조생', '천향', '진미', '수미', '미스홍', '유미')을 육성하였다. 국립원예특작과학원 과수과에서는 육성 품종의 보호와 특허권을 확보하기 위해 분자생물학적 마커의 개발이 필요하여, 235 세트의 Operon 프라이머를 이용하여 품종 특이적인 DNA 절편 134개를 확보했고, 염기서열 분석을 통해 SCAR 마커를 개발하였다. 개발된 마커 14 세트를 이용하여 육성 품종과 육성 품종의 모본 부본이 포함된 30품종에 대해 구분이 가능하였다. 이 결과를 토대로 국립원예특작과학원 과수과에서 육성한 품종에 대한 분자생물학적 근거로 특허권을 확보하고, 묘목시장에서 품종에 대한 정확한 근거를 제시할 것으로 기대된다.

Keywords

Acknowledgement

Supported by : 국립원예특작과학원

References

  1. Chang KY. 1989. Old References Related to Agriculture in Korea. Korean J. Breed. 21(3):234-240.
  2. Chung KH, Kang SJ, Jun JH, Okie WR. 1998. Stone fruit production and breeding in Korea. Fruit Varieties J. 52(4) :184-189.
  3. Doyle JJ, Doyle JL. 1990. Isolation of plant DNA from fresh tissue. Focus. 12(1):13-15.
  4. FAOSTAT(2007) http://www.faostat.fao.org
  5. Fideghelli CG, Della Strada F, Grassi F, Morico G, Monet R. 1998. The peach industry in the world: Present situation and trend. Acta Hort. 465:29-40.
  6. Kang SJ, Kim SB, Shin YU, Cho MD, Kim WC, Kim JH, Kim YK. 1986. Breading of a new peach cultivar 'Baekmijosaeng' having high quality and early ripening characteristics. Res. Rept. Rural Dev. Adimn., Hort. And Agric. Eng. (Hort.) pp.53-56.
  7. Kim YH, Cho MD, Lee DK, Lee CS, Yu YS. 1978.The new peach cultivar 'Yumyeong'. Res. Rept. Rural Dev. Adimn., Hort. And Agric. Eng. (Hort.) pp.1-5.
  8. Ryugo K. 1988. Fruit culture. John Wiley & Sons, Inc. Univ. of California Davis, California. pp. 261-263.
  9. Scorza R, Hammerschlag FA. 1992. Stone fruits. Biotechnology in agriculture 8:277-301.