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Identification of new Breeding Lines by Prunus Persica Cultivar-Specific SCAR Primers  

Han, Sang Eun (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Cho, Kang-Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Nam, Eun Young (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Shin, Il-Sheob (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Kim, Chung Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Kim, Hyun Ran (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Kim, Dae-Hyun (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Publication Information
Korean Journal of Breeding Science / v.42, no.5, 2010 , pp. 495-501 More about this Journal
Abstract
Peaches (Prunus persica) are less popular than the fresh fruits, because their flesh gets soft faster. So many breeders focused on their aim to firmness. Other breeders focused on juiciness, flavor and aroma. Breeding requires much labor, time and money. To reduce these requirements, many scientists develop many SSR, CAPS and SCAR makers. New peach varieties bred in our National Institute of Horticultural & Herbal Science (NIHHS) such as, Cheonhong, Suhong and Harhong are yellow flesh cultivars and Yumyeong, Baekmijosaeng, Baekhyang, Jinmi, Soomee, Mihong, Misshong and Yumee are white flesh cultivars. These peach cultivars are planted in orchard of Korea. To assert breeding cultivar patents and prevent patent disputes, we detected cultivar-specific DNA fragment using 235 sets of Operon RAPD primers, analyzed 134 DNA sequences and constructed SCAR primers. To confirm the cultivar-specific SCAR markers, we applied candidate SCAR primers to 30 peach cultivars widely cultivated in Korea. These selected lines are included father and mother lines that were used to develop new varieties in NIHHS. Using fourteen SCAR primer sets, we characterized thirty cultivars selected. The SCAR marker is expected to serve as molecular evidence distinguishing different peach varieties.
Keywords
SCAR marker; Prunus persica; Peach;
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