• Journal of Life Science
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• v.17 no.10
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• pp.1426-1433
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• 2007

To investigate the antibiotic resistant patterns of the bacteria producing ESBL, we isolated one organism of Klebsiella pneumoniae from a clinical laboratory in Busan. The organism that produces ESBL gene was detected by double disk synergy test and the presence of three ESBL genes (TEM-1, SHV-12, CTX-M-15) was confirmed by polymerase chain reaction and DNA sequencing analysis. To analyse the characteristics of three ESBL genes, we performed transconjugation, transformation and cloning experiment with the organism. The MIC of Klebsiella pneumoniae was revealed that ceftazidime, cefotaxime and ceftriaxone were $256\;{\mu}g/ml,\;128\;{\mu}g/ml\;and\;128\;{\mu}g/ml$ respectively. The MIC of conjugant (E. coli $RG176^{Na(r)}$) af was revealed that ceftazidime, cefotaxime and ceftriaxone were $256\;{\mu}g/ml,\;64\;{\mu}g/ml\;and\;128\;{\mu}g/ml$ respectively. The MIC of transformant (E. cofi $DH5{\alpha}$) was revealed that ceftazidime, cefotaxime and ceftriaxone were $128\;{\mu}g/ml,\;32\;{\mu}g/ml,\;and\;32\;{\mu}g/ml$ respectively, The MIC of cloned organism of SHV-12 gene (E. coli $DH5{\alpha}$) was revealed that ceftazidime, cefotaxime and ceftriaxone were $128\;{\mu}g/ml,\;8\;{\mu}g/ml,\;and\;32\;{\mu}g/ml$ respectively. The results indicated that MIC of conjugant was higher than MIC of transformant and also SHV-12 gene were not resistant against cefotaxime antibiotic.

• Applied Microscopy
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• v.39 no.3
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• pp.237-243
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• 2009

Chinese minnow, Rhynchocypris oxycephalus is a teleost belonging to Leuciscinae, Cyprinidae. The oogenesis and ultrastructure of egg envelope in Chinese minnow were investigated by light and electron microscopes. The ovary was of white yellowish and ellipsoidal shape with the major axis 30 mm and the minor axis 7mm. Cytoplasm of oogonia was basophilic and many nucleoli were located at inside of nuclear membrane. In primary oocytes, yolk vesicles were distributed only in the marginal area and egg envelope was not formed on the outside of an egg. In secondary oocytes, the egg envelope was formed and yolk vesicles in the cytoplasm were increased than the earlier stage. The basophilic substance of cytoplasm was changed to acidic. In case of matured egg, thickness of egg envelope and size of egg were increased. The yolk vesicles were changed to yolk mass in accordance with development. The outer surface of egg envelope was covered by microvilli-structures, and had a micropyle on the area of animal pole. Egg envelope consisted with 2 layers, an adhesive outer layer with microvilli-structures and fibrillar inner layer. In conclusion, the oogenesis of Chinese minnow was characterized by the increase in cell size, the formation and accumulation of yolk, and the decrease of basophilic substance in the cytoplasm. The oogenesis of Chinese minnow seems to share common patterns in Cyprinidae, but these ultrastructural unique characters of egg envelope can be utilized in taxonomy of teleost.

• Applied Biological Chemistry
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• v.47 no.2
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• pp.182-186
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• 2004

Morphological observation of roots and molecular technique were used to investigate the symbiotic relationships between arbuscular mycorrhizal (AM) fungi and ginseng roots. Korean ginseng, Panax ginseng, was collected from 8 sites in Korea. Colonization pattern of AM fungi in ginseng roots was determined as an Arum type under light microscopes. Nested PCR using AM fungal specific primers was employed to amplify a partial region on 18s rDNA of AM fungi from the root extracted mixed DNA. The amplified DNA was cloned and analyzed by random fragment length polymorphism (RFLP) with restriction enzymes, AluI, HinfI and AsuC21. One from each RFLP pattern was selected for sequencing. A total 16 clones were sequenced and identified as 2 species of AM fungi; Paraglomus brasilianum and Glomus spurcum. Paramglomus brasilianum was found from most of the ginseng roots, in this syudy suggesting that this species of AM fungi could have specific relationship with the ginseng root. Possible roles of AM fungal species in ginseng roots are discussed.

• Korea journal of population studies
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• v.32 no.1
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• pp.25-50
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• 2009

Using the Korean labor and Income Panel Study, this study examines the impact of educational attainment on first marriage formation in Korea. In particular, this study examines whether higher education is associated primarily with delayed marriage or with a higher likelihood of never marrying. The results show that the trend toward later and less marriage is occurring at all levels of educational attainment in Korea. The data also indicated that educational attainment showed differential effects on the risk of first marriage formation for Korean men and women. For those born before 1970, both highly educated men and women delayed marriage but caught up by marrying at higher rates at later ages. However, for those born after 1970, highly educated women were increasingly more likely to show the trend toward later and less marriage, while highly educated men were more likely to delay marriage but caught up by marrying at higher rates at later ages. Overall the evidence in this study is consistent with the argument that gender divisions make it difficult for women to balance work and family in Korea.

• Korean Journal of Food Science and Technology
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• v.34 no.4
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• pp.625-630
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• 2002

Raw pine-mushrooms (Tricholoma matsutake Sing.) of four grades and those frozen were analyzed for proximate composition, smell pattern, volatile flavor compounds, and sensory evaluation. Proximate compositions of raw pine-mushrooms (A-C, regular grade) were $89.48{\sim}90.77%$ moisture, 6.81% ash excluding D (below regular grade) sample, $2.24{\sim}2.52%$ crude lipid, and $16.19{\sim}20.01%$ crude protein. Proximate compositions of frozen pine-mushrooms preserved for 6 months at -20 and $-70^{\circ}C$ showed no difference compared with raw pine-mushrooms. Results of smell pattern and multidimensional analysis revealed raw pine-mushrooms showed no differences among samples, but frozen pine-mushrooms differed significantly depending on the grade. Volatile flavor compounds of pine-mushrooms were analyzed using a purge and trap method with GC/MSD. Twenty-nine volatile compounds were identified, among which alcohols such as 1-octen-3-ol, 2-octen-1-ol, 3-methyl-butanol, and n-octanol were commonly found in all pine-mushroom samples. In sensory attributes, raw pine-mushrooms were not significantly different at 5% level, and sample D of frozen pine-mushrooms scored lower than samples $A{\sim}C$.

• Proceedings of the Korea Water Resources Association Conference
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• 2016.05a
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• pp.209-210
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• 2016

우리나라의 연평균강수량은 약 1362 mm이며, 총강수량의 약 30% 이상이 증발산을 통해 손실되고 있다고 추정되어지고 있다. 증발산은 물 수지 분석에 있어 매우 중요한 성분이며, 많은 부분을 차지하지만 다른 요인들에 비해 직접적인 관측이 어려워 과거에는 경험식을 사용하거나 단순하게 가정에 의해 결정해 왔다. 또한 기상자료로부터 증발산량을 추정하거나 증발접시나 추정식으로 잠재증발산을 추정하고 있다. 또한 최근 기후변화의 가속화에 따른 홍수의 가뭄의 강도와 빈도가 높아지고 있으며, 이에 따라 수자원 관리에 있어서 기초수문조사 항목에 많은 변화를 요구하고 있다. 그 결과 2007년 4월 하천법 개정으로 증발산량 및 토양수분량이 기초수문조사 항목으로 추가되었으며, K-water 연구원에서는 용담시험유역에 플럭스타워를 설치하였고 현재 운영 중에 있다. 덕유산 플럭스타워는 용담시험유역 내에 위치한 금강 수계 구량천 상류부의 덕곡제 유역 내에 설치하였으며, 2011년 4월부터 실제 증발산량을 관측하고 있다. 동경 $127^{\circ}$42'23" ~ $127^{\circ}$44'53", 북위 $35^{\circ}$50'47" ~ $35^{\circ}$52'50"사이로 중부지방에 위치한 유일한 증발산관측 타워이다. 유역 면적은 9.27 km2으로 유로연장 3.48 km, 유역 평균폭 2.66 km, 형상계수는 0.77이며, 덕곡제플럭스 타워 주변의 토지이용은 대부분 산림으로 구성되어 있으며, 침활 혼효림과 낙엽송림으로 임상 분포가 이루어져 있다. 주요 관측기기로는 3차원 풍향 풍속계, $CO_2/H_2O$ 기체분석기, 순복사 측정 센서, 지중열플럭스 측정 센서 등이 있다. 2011년부터 측정된 자료를 바탕으로 에디공분산 방법을 이용하여 증발산량을 측정하였으며, 30분간의 데이터 18,000개 중 취득률 90 % 이상의 데이터를 대상을 분석을 실시하였다. 2011 ~ 2015년도 증발산량 분석 결과는 아래의 표와 같다. 증발산의 패턴은 1월부터 서서히 증가하지만 활발하지는 않고, 4월부터 매우 활발해져 8월에 최대치에 이른다. 10월부터 증발산량은 급격히 감소하기 시작하며 11, 12월에는 증발산이 거의 발생하지 않는 공통적인 경향을 보였다. 2013년 8, 9월은 다른 해와 다른 경향을 보이고 있는데, 이는 2013년 8, 9월에 강우가 많이 발생하여 증발산량이 감소하였기 때문으로 판단된다. 2015년 8월은 다른 년도와 비교했을 때, 매우 높은 증발산량을 보이는데 이는 2015년 8월에 많은 강우에도 식생이 활발하게 작용하였기 때문으로 판단된다.

• Education of Primary School Mathematics
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• v.24 no.4
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• pp.189-202
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• 2021

The teacher's questions presented in the problem-solving situation stimulate students' mathematical thinking and lead them to find a solution to the given problem situation. In this research, the types and functions of questions presented in chapters of Pattern area of the 2015 revised elementary school mathematics textbooks were compared and analyzed by grade cluster. Through this, it was attempted to obtain implications for teaching and learning in identifying the characteristics of questions and effectively using the questions when teaching Pattern area. As a result of this research, as grade clsuter increased, the number of questions per lesson presented in Pattern area increased. Frequency of the types of questions in textbooks was found to be high in the order of reasoning questions, factual questions, and open questions in common by grade cluster. In chapters of Pattern area, relatively many questions were presented that serve as functions to help guess, invent, and solve problems or to help mathematical reasoning in the process of finding rules. It can be inferred that these types of questions and their functions are related to the learning content by grade cluster and characteristics of grade cluster. Therefore, the results of this research can contribute to providing a reference material for devising questions when teaching Pattern area and further to the development of teaching and learning in Pattern area.

• 재활복지
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• v.20 no.4
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• pp.79-106
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• 2016

This study examined the meanings of the disability education of the mothers who reared their children with brain lesions. For this purpose, Rennie's hermeneutic grounded theory was applied and the consented 7 mothers participated in this study. With the in-depth interviews, 53 meaning units, 16 subordinate categories and 7 hermeneutic categories were classified. These 7 hermeneutic categories were 'wailing miserably everyday', 'social mobilization of the surroundings', 'straight forward', 'smash rock with the eggs', 'looking at a faraway', 'learning together' and 'subjectivation of disability education.' The experience of disabled children education process was concurrent experience of frustration and hoping that moving toward a big hope through the resignation stage, the chasing stage, the vision stage, the challenge stage, and the small achievement stage. Repetitive common patterns of behavior revealed three types: wishy-washy type, realistic-strategy type, and indomitable-challenge type. Moreover, the core category of educational experience was concluded to be 'a pedagogical process of turning despair from severe disabilities into hope through education.' Based on the analysis results, concrete intervention plans for social welfare practice were suggested to support the disabled children's lives with high quality of education.

• Korean Journal of Food Science and Technology
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• v.51 no.1
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• pp.42-47
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• 2019

The purpose of this study was to compare the amygdalin content, alcohol content, pH, titratable acidity, and color of maesil wine prepared with two different manufacturing processes. Maesil wine was made from maesil chung, and maesil was preserved (MW1) or removed (MW2) before fermentation. During aging, amygdalin content in all the wines was gradually reduced, and the content of MW2 was less than that of the other. The alcohol content, pH, and titratable acidity ranged from around 9.87-10.94, 3.57-3.80, and 8.89-10.68%, respectively. The difference between the samples was not significant. For color, MW1 had lower L and higher a and b values than MW2. In this study, the difference in physicochemical properties according to the presence of maesil was not significant, indicating no degradation of the quality according to the manufacturing processes. However, the MW2 showed a reduction in amygdalin contents.

• Journal of Navigation and Port Research
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• v.44 no.6
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• pp.447-452
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• 2020

Extratropical cyclone in winter season is very important in safe operation of ships because it is a major cause of marine accidents due to its strong power. In this study, we used meteorological data, to analyze extratropical cyclones occurring near the 1st Pacific polar front from December 2019 to February 2020. The analysis results are as follows. During those three months, we recorded 41 extratropical cyclones, 8 of which were remarkably developed. The central pressure of the strongest cyclone was 947hPa. The highest number of cyclones were generated in the East P acific Ocean around J apan (16), followed by the areas around Korea, the East China Sea, and the southern Sea of J apan. The cyclones followed five major tracks with a common northeast pattern. We thus concluded that the optimal route for a ship encountering an extratropical cyclone in the North P acific in winter would be south of the cyclone's center traveling eastbound and north of the center traveling westbound.