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Identification of Arbuscular Mycorrhizal Fungi Colonizing Panax ginseng Using 18S rDNA Sequence  

Eo, Ju-Kyeong (Department of Biology Education, Korea National University of Education)
Kim, Dong-Hun (Department of Biology Education, Korea National University of Education)
Jeong, Hyeon-Suk (Department of Biology Education, Korea National University of Education)
Eom, Ahn-Heum (Institute of Natural Science, Korea National University of Education)
Publication Information
Applied Biological Chemistry / v.47, no.2, 2004 , pp. 182-186 More about this Journal
Abstract
Morphological observation of roots and molecular technique were used to investigate the symbiotic relationships between arbuscular mycorrhizal (AM) fungi and ginseng roots. Korean ginseng, Panax ginseng, was collected from 8 sites in Korea. Colonization pattern of AM fungi in ginseng roots was determined as an Arum type under light microscopes. Nested PCR using AM fungal specific primers was employed to amplify a partial region on 18s rDNA of AM fungi from the root extracted mixed DNA. The amplified DNA was cloned and analyzed by random fragment length polymorphism (RFLP) with restriction enzymes, AluI, HinfI and AsuC21. One from each RFLP pattern was selected for sequencing. A total 16 clones were sequenced and identified as 2 species of AM fungi; Paraglomus brasilianum and Glomus spurcum. Paramglomus brasilianum was found from most of the ginseng roots, in this syudy suggesting that this species of AM fungi could have specific relationship with the ginseng root. Possible roles of AM fungal species in ginseng roots are discussed.
Keywords
arbuscular mycorrhizal fungi; Glomus spurcum; Panax ginseng; Paraglomus brazilianum; RFLP;
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1 Elmes, R. P. and Mosse, B. (1984) Vesicular-arbuscular endomycorrhizal inoculum production 2. Experiments with maize zea-mays and other hosts in nutrient flow culture. Can. J Bot. 62, 1531-1536   DOI
2 Helgason, T., Daniell, T. J., Husband, R, Fitter, A. H. and Young, J. P. W. (1998) Ploughing up the wood-wide web? Nature 394, 431   DOI   ScienceOn
3 Mosse, B. (1973) Plant Growth Responses to Vesicular Arbuscular Mycorrhiza Part 4 in Soil Given Additional Phosphate. New Phytologist 72, 127-136   DOI   ScienceOn
4 Redecker, D., ThierfeIder, H., Walker, C. and Werner, D. (1997) Restriction analysis of PCR-amplified internal transcribed spacers of ribosomal DNA as a tool for species identification in different genera of the order glomales. Appl. Environ. MicrobioI. 63, 1756-1761
5 Schenck, N. C. and Perez, Y. (1990) In Manual for the Identification of VA Mycorrhizal Fungi. Synergistic Publications, Gainesville, Florida. pp. 1-286
6 Smith, F. A. and Smith, S. E. (1997) Tansley review No. 96 structural diversity in (vesicular)-arbuscular mycorrhizal symbioses. New Phytologist 137, 373-388   DOI   ScienceOn
7 Merryweather, J. and Fitter, A (1998) The arbuscular mycorrhizal fungi of Hyacinthoides non-scripta: I. Diversity of fungal taxa. New Phytologist 138, 117-129   DOI   ScienceOn
8 Yeoman, M. M. and Yeoman, C. L. (1996) Tans1ey Review No.90. Manipulating scondary metabolism in cultured plant cells. New Phytologist 134, 553-569   DOI   ScienceOn
9 vanTuinen, D., Jacquot, E., Zhao, B., Gollotte, A and Gianinazzi, P. V. (1998) Characterization of root colonization profiles by a microcosm community of arbuscu1ar mycorrhizal fungi using 25S rDNA-targeted nested PCR. Molecular Ecology 7, 879-887.   DOI   ScienceOn
10 Menge, J. A, Raski, D. J., Lider, L. A, Johnson, E. L. v., Jones, N. 0., Kissler, J. J. and Hemstreet, C. L. (1983) Interactions between mycorrhizal fungi soil fumigation and growth of grapes in California USA Am. J. Enology Viticulture 34, 117-121
11 Redecker, D. (2000) Specific PCR primers to identify arbuscu1ar mycorrhizal fungi within colonized roots. Mycorrhiza 10, 73-80   DOI   ScienceOn
12 Safir, G. R., Boyer, J. S. and Gerdemann, J. W. (1971) Mycorrhizal enhancement of water transport in soy bean-D. Science 172, 581-582   DOI   PUBMED   ScienceOn
13 Ausubel, F. M., Brent, R, Kingston, R E., Moore, D. D., Seidman, J. G., Smith, J. A. and Struhl, K. (1999) In Short protocols in molecular biology. John Wiley and Sons, Inc, New York
14 Abbas, J. D., Hetrick, B. A. D. and Jurgenson, J. E. (1996) Isolate specific detection of mycorrhizal fungi using genome specific primer pairs. Mycologia 88, 939-946   DOI   ScienceOn
15 Thompson, J. D., Higgins, D. G. and Gibson, T. J. (1994) CLUSTAL X: Improving the sensitivity of multiple sequence alignment though sequence weighting, position-specific gap penalties and wieght matrix choice. Nucleic Acid Res. 26, 179-182
16 Dehne, H. W. and Backhaus, G. F. (1986) The use of vesiculararbuscular mycorrhizal fungi in plant production I. Inoculum production. Zeitschrift fiter Pflanzenkrankheiten Pflanzenschutz 93, 415-424
17 Rosendahl, S. and Taylor, J. W. (1997) Development of multiple genetic markers for studies of genetic variation in arbuscular mycorrhizal fungi using AFLP. Mol. Ecol. 6, 821-829
18 Tae, M. S. (2002) In Molecular and morphological diversity of arbuscular mycorrhizal fungi in Mt. Work. Korea National University of Education, Chungbuk
19 Bonfante, P. and Perotto, S. (1995) Tansley Review No. 82: Strategies of arbuscular mycorrhizal fungi when infecting host plants. New Phytologist 130, 3-21   DOI   ScienceOn
20 Schuessler, A, Gehrig, H., Schwarzott, D. and Walker, C. (2001) Analysis of partial Glomales SSU rRNA gene sequences: implications ofr primer design and phylogeny. Mycol. Res. 105, 5-15   DOI   ScienceOn
21 Lee, J. K., Tae, M. S., Eom, A H. and Lee, S. S. (2003) Restriction analyses of PCR amplified partial SSU ribosomal DNA to distinguish arbuscular mycorrhizal fungi from other fungi colonizing plant roots. Mycobiology 31, 68-73   DOI
22 Gildon, A. and Tinker, P. B. (1983) Interactions of vesicular arbuscular mycorrhizal infections and heavy metals in plants 2. The effects of infection on uptake of copper. New Phytologist 95, 263-268   DOI   ScienceOn
23 Trappe, J. M. (1981) In Advance in food producing systems for arid simiarid lands.: Mycorrhizae and productivity of arid and semiarid rangelands. pp. 581-599
24 Koske, R. and Gemma, J. N. (1989) A modified procedure for staining roots to detect VA mycorrhizas. Mycol. Res. 92, 486-505   DOI
25 Lee, J. K. and Eom, A. H. (2003) Molecular identification of arbuscular mycorrhizal fungi colonizing plant roots. in press.
26 Pond, E. C., Menge, J. A. and Jarrell, W. M. (1984) Improved growth of tomato in salinized soil by vesicular arbuscular mycorrhizal fungi collected from saline soils. Mycologia 76, 74-84   DOI
27 Busse, M. D. and Ellis, J. R (1985) Vesicular-Arbuscular Mycorrhizal Glomus-Fasciculatum Influence on Soybean Glycine-Max Drought Tolerance in High Phosphorus Soil. Can. J. Bot. 63, 2290-2294   DOI