• Title/Summary/Keyword: 공초점현미경

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THE ANALYSIS OF REMINERALIZATION EFFECT IN FLUORIDE VARNISH USING CONFOCAL LASER SCANNING MICROSCOPE (공초점 레이저 주사 현미경을 이용한 불소 바니쉬 재광화 효과의 분석)

  • Kwon, Ji-Hoon;Park, Ho-Won;Lee, Ju-Hyun;Seo, Hyun-Woo
    • Journal of the korean academy of Pediatric Dentistry
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    • v.35 no.1
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    • pp.57-64
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    • 2008
  • It is well established that fluoride products play an important role in the prevention and remineralization of carious lesion. Fluoride varnish is a concentrated topical fluoride and varnishes adhere to tooth surface, permitting prolonged fluoride exposure and uptake. In this study, the artificial initial enamel caries was caused on the sound human enamel and divided 60 specimens into three groups. Group 1 and group 2 were treated with the topical application of fluoride varnish and stored in artificial saliva for 1 and 2 weeks. Group 3 was stored in artificial saliva for 2 weeks, which acted as control group. Changes in mineral contents were analysed with the confocal laser scanning microscope. The following results were obtained: 1. In group 1 and group 2, the total fluorescence of the lesion(TFL) was reduced in remineralized area compared to in demineralized area(p<0.05). 2. The total fluorescence of the lesion of remineralized area was more reduced in group 2 than in group 1(p<0.05). 3. The total fluorescence of the lesion was more reduced in group 2 than in control group(p<0.05). 4. Confocal laser scanning microscope can be used in quantitative analysis of remineralization by fluoride varnish.

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Investigation of Bordered Pit Ultrastructure in Tracheid of Korean Red Pine (Pinus densiflora) by Confocal Reflection Microscopy (공초점반사현미경을 이용한 소나무 유연벽공의 초미세구조 연구)

  • Kwon, Ohkyung
    • Journal of the Korean Wood Science and Technology
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    • v.42 no.3
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    • pp.346-355
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    • 2014
  • Confocal reflection microscopy (CRM) was utilized to create 3-dimensional images of bordered pits and cell wall in the tracheid of Korean red pine (Pinus densiflora). Ultrastructures of torus, margo, and pit border were clearly observable in the CRM micrograph. Micrograph of cross-field pit revealed the connecting and supporting structure between tracheid and ray parenchyma cell. The CRM micrographs enabled to investigate detailed structures of tracheid cell wall such as S1, S2, S3 layers, transition layers between these layers, and microfibril (MF) orientation in S3 and S2 layers as well as complicated distribution of MF orientation around bordered pits. Not only concentric MF orientation of border thickening in the pit border was observed, but also changes in MF orientation from the cell wall to the border. From the experimental results, the CRM was thought to be a versatile microtechnique to investigate detailed structures of cell wall and bordered pit in the tracheid and cross-field pit between tracheid and ray parenchyma cell.

Proposal and design of reflecting optical system to improve detection intensity in fluorescence confocal scanning microscopy (형광 공초점 주사 현미경의 측정 강도 향상을 위한 반사 광학계의 제안 및 설계)

  • 강동균;서정우;권대갑
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2002.05a
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    • pp.187-190
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    • 2002
  • Confocal microscopy is very popular technology in bio-medical inspection due to its ability to reject background signals and to measure very thin slide of thick specimens, which is called optical sectioning. But intensity of detected signal in fluorescence type confocal microscopy is so small that only 0.2% of emitted fluorescence light can be detected in the best case. In this paper, we proposed the reflecting optical system to improve the detection intensity and designed the optical system by optimal design method. At the end of the paper, we analyzed the characteristics of the proposed reflecting optical system.

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A Study on Confocal Microscope for A Precise 3-Dimensional Surface Measurement (물체표면의 3차원 정밀형상측정을 위한 공초점 현미경에 관한 연구)

  • 송대호;안중근;강영준;채희창
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 1997.10a
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    • pp.233-236
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    • 1997
  • In modem industry, the accuracy and the surface-finish requirements for machined parts have been becoming ever more stringent. Optical method in measurements is playing an important role in vibration measurement, crack and defect detection and surface topography with the advent of opto-mechatronics. In this study, the principle of the general confocal microscope is introduced for surface measurement, and the advanced confocal microscope that has better measuring speed than the traditional confocal microscope is developed. A study on improving the resolution of the advanced confocal microscope is followed. Finally, Software for data acquisition and analysis of various parameters in surface geometrical features has been developed.

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Error Analysis and Alignment Tolerancing for Confocal Scanning Microscope using Monte Carlo Method (Monte Carlo 방법을 이용한 공초점 주사 현미경의 오차 분석과 정렬 공차 할당에 관한 연구)

  • 유홍기;강동균;이승우;권대갑
    • Journal of the Korean Society for Precision Engineering
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    • v.21 no.2
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    • pp.92-99
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    • 2004
  • The errors can cause the serious loss of the performance of a precision machine system. In this paper, we proposed the method of allocating the alignment tolerances of the parts and applied this method to get the optimal tolerances of a Confocal Scanning Microscope. In general, tight tolerances are required to maintain the performance of a system, but a high cost of manufacturing and assembling is required to preserve the tight tolerances. The purpose of allocating the optimal tolerances is minimizing the cost while keeping the high performance of the system. In the optimal problem, we maximized the tolerances while maintaining the performance requirements. The Monte Carlo Method, a statistical simulation method, is used in tolerance analysis. Alignment tolerances of optical components of the confocal scanning microscope are optimized to minimize the cost and to maintain the observation performance of the microscope. We can also apply this method to the other precision machine system.

A Study on the Con-focal Microscope for the Surface Measurements (공초점 현미경을 이용한 물체표면 형상측정에 관한 연구)

  • 강영준;송대호;유원재;백성훈
    • Journal of the Korean Society for Precision Engineering
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    • v.20 no.5
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    • pp.73-81
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    • 2003
  • In case of hollow cylinder extrusion using porthole die, the effects of extrusion parameters-temperature, the speed of extrusion, the shape of the die and mandrel-on metal flow in porthole die extrusion of aluminum have been investigated. However, there have been few studies about condenser tube extruded by porthole die. Original metal flow of condenser tube by porthole die extrusion is similar to hollow cylinder extrusion but the estimation of metal flow for extrusion parameters is different. For example, variation of chamber length in hollow extrusion only affects the welding pressure, however, the welding chamber length in condenser tube extrusion influences to the welding pressure as well as the deflection of mandrel. This study was designed to evaluate metal flow, welding pressure, extrusion load, tendency of mandrel deflection according to angular variation in the bottom of chamber in porthole die. Estimation was carried out using finite element method in as non-steady state. Analytical results can provide useful information the optimal design of porthole die.