• Research in Plant Disease
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• v.27 no.4
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• pp.172-179
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• 2021

This study aimed to assess the disease incidence and distribution of toxigenic in Korean triticale. The pathogen of triticale that cause Fusarium head blight were isolated from five different triticale cultivars that cultivated in Suwon Korea at 2021 year. The 72 candidate were classified as a Fusarium asiaticum by morphology analysis and by ITS1, TEF-1α gene sequence analysis. And the results of pathogenicity with 72 isolates on seedling triticale, 71 isolates were showed disease symptom. Also, seven out of 71 Fusarium isolates were inoculated on the wheat, to test the pathogenicity on the different host. The results showed more low pathogenicity on the wheat than triticale. The results of analysis of toxin type with 72 isolates, 64.6% isolates were produced nivalenol type toxin and other 4.6% and 30.8% isolates were produce 3-acetyldeoxynivalenol and 15-acetyldeoxynivalenol, respectively. To select fungicide for control, the 72 Fusarium isolates were cultivated on the media that containing four kinds fungicide. The captan, hexaconazole, and difenoconazole·propiconazole treated Fusarium isolates were not showed resistance response against each fungicide. However, six isolates out of 72 isolates, showed resistance response to fludioxonil. This study is first report that F. asiaticum causes Fusarium head blight disease of triticale in Korea.

• Journal of The Korean Society of Grassland and Forage Science
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• v.42 no.4
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• pp.229-236
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• 2022

This study was conducted to evaluate the efficacy of adding the microbial inoculants to silage for reducing mycotoxins in rice straw silage. When a single agent of L. plantarum and a mixed agent of L. plantarum and S. cerevisiae were added in rice straw silage contaminated mycotoxins, it had an effect on silage fermentation and fiber degradation as well as mycotoxin reduction. Among the mycotoxins, only ochratoxin A and zearalenone were found in the test sample. Ochratoxin A and zearalenone showed a decreasing trend with the addition of silage inoculants compared to the control groups (38.11±2.22 and 633.67±50.30 ㎍/kg), and there was a significant difference at the mixed agents; 27.78±2.28 and 392.72±25.04 ㎍/kg, respectively (p<0.05). The pH was lower in the single agent and the mixed agent compared to the control (p<0.05). The concentration of lactic acid was higher in the single agent (11.73±0.31 mM) than in the control group (8.18±0.93 mM), and the highest concentration was 16.01±0.88 mM in the mixed agent (p<0.05). Acetic acid and propionic acid were found to be significantly lowered with the addition of silage inoculants (p<0.05). Total VFA was also lower at the addition of silage inoculants than the control group (p<0.05). The rumen in situ dry matter degradation of NDF and ADF was maintained at the highest levels of the mixed agent during the culture period, followed by the single agent and the control group at the lowest level. NDF and ADF degradation showed a significant difference at all time points after 12 and 24 hours of culture, respectively (p<0.05). The study results showed that the silage inoculants had the positive effects on quality increasing of rice straw silage; fermentative charateristics, fiber degradation and mycotoxins reduction. Ochratoxin A and zearalenone were greater reduction by adding bacterial inoculants of silage. Therefore it is considered that L. plantarum and S. cerevisiae will improve the quality and stability with remediation of mycotoxin in silage.

• Monthly Korean Chicken
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• s.138
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• pp.122-127
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• 2006

도계장에서는 항상 비품이 발생한다. 골절, 피부병변, 착색이상 등 여러 가지 상태의 계육이 비품으로 분류되어 큰피해를 입고 있다. 가까운 예로 미국의 경우 이에 대한 피해액이 연간 5천만 달러로 추산되고 있다. 국내에서는 이러한 문제 때문에 육계 담당 부서에서는 항상 비품에 대해서 스트레스를 받고 있다. 하지만 이러한 문제들이 정말 스트레스가 되는 원인은 사육 중에 발견되기보다는 도계 후에 발견되며, 이에 따라상품가치가 현저히 감소하고 추가적인 작업이 요구되기 때문일 것이다. 대부분의 회사들이 이 문제를 접하게 되면, 주로 곰팡이 독소 흡착제를 사용하는 것이 유일한 대책인 것으로 생각한다. 이런 경우엔 약품에 대한 효과는 잘 모르겠지만, 필자의 느낌으로는 일정한 시간이 흘러 자연스럽게(?) 해결되는 과정을 걷는 듯 하며, 관련 부서에서는 이 약제의 효과에 대해 판단하기보다는 일단 직면한 문제가 해결되었 다고 결과론적으로 만족하는 듯 하다. 필자는 이 지면을 통해 그 동안의 관점과는 다른 측면에서 이런 문제의 원인과 그 대책에 대해 간략히 논하고자한다. 도계장에서는 항상 여러 가지 비품들이 발생한다. 이 모든 것들을 논하기에는 너무 범위가 넓기 때문에 여기서는 독자들의 이해를 위해 비품들 중에서 등 부위의 상처와 복부의 상처(배꼽 닭)에 대해 국한하도록 하겠다. 아마도 이 부분외의 다른 비품들은 대부분 그 원인을 쉽게 추정할 수 있기 때문이다. 먼저, 독자들에게 질문하고 싶은 것이 있다. 이러한 비품들의 직접적인 원인은 무엇인가? 앞서 얘기한 것과 같이 각 회사들의 대책으로 이용되는 곰팡이 독소 흡착제를 보면, 곰팡이 독소가 가장 직접적인 원인으로 생각할 수도 있다. 하지만 결론적으로 직접적인 원인은 외상이다. 즉, 외부의 물리적인 작용에 의한 상처인 것이다. 이런 측면에서 볼 때 가장 직접적인 원인은 외상이며, 이 외에 깃털발육 이상(혹은 저하), 바닥상태 불량 등과 같은 사양관리 이상과 면역 억제성 질환 및 지혈능력의 저하 등이 직접적인 원인으로 인한 문제들을 더욱 악화시키는 간접적인 원인으로 작용한다.

• Research in Plant Disease
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• v.24 no.4
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• pp.308-312
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• 2018

We analyzed rice sample with scab (collected in 2017) for fungal occurrence and found that frequency of Fusarium spp. was the highest among fungal genera. Within Fusarium, Fusarium asiaticum-nivalenol type dominated as 79%. Among mycotoxins, zearalenone was the highest toxin detected (1117 ng/g), followed by deoxynivalenol (163 ng/g), 15-acetyl deoxynivalenol (155 ng/g), nivalenol (110 ng/g). Analysis of mycotoxin levels in scabby and healthy grains (collected in 2015) revealed that both grain samples were contaminated with zearalenone. However, level difference of zearalenone between the samples was over 5 times as the scabby samples were 1400-2370 ng/g, while healthy ones were 240-430 ng/g. This result indicates that scabby grains should be removed when harvest to reduce zearalenone contamination in rice.

• Safe Food
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• v.7 no.3
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• pp.60-70
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• 2012

• Safe Food
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• v.1 no.3
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• pp.29-37
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• 2006

• Safe Food
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• v.1 no.3
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• pp.19-28
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• 2006

• Journal of Food Hygiene and Safety
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• v.33 no.5
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• pp.399-403
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• 2018

In this study, safety investigations on harmful microorganisms and mycotoxins were conducted on honeybee drone pupae as a new food material, which is rich in nutrients and capable of being mass produced in apiaries. The honeybee drone pupae produced in apiaries were collected from three different regions in Korea and frozen immediately. Subsequently, the samples were subjected to freeze-drying. According to the Korean Food Code test method, coliforms, Salmonella species, Staphylococcus aureus, and enterohemorrhagic Escherichia coli were not detected in 280 honeybee drone pupas. In addition, mycotoxins, aflatoxin $B_1$, ochratoxin A, deoxynivalenol, and zearalenone were not detected. Therefore, it is proposed that the honeybee drone pupae collected from the beehives and immediately frozen as safe from harmful microorganisms and mycotoxins and can be used as a food material.

• Research in Plant Disease
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• v.17 no.3
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• pp.272-279
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• 2011

Fusarium species are worldwide causal agents of Fusarium head blight (FHB) in cereals such as barley and wheat. Their toxigenic potential is a health risk for both humans and animals. To survey the natural occurrence of FHB and mycotoxins produced by Fusarium, total 126 barley or wheat grains grown in 2009-2010 season in Korea were collected. The incidence of FHB was 30.7% in silage barley, 26.9% in wheat, 20.7% in naked barley, 19.4% in malting barley, 16.4% in unhulled barley. Overall FHB incidence of barley and wheat in 2010 was 23.0% and 10% higher than that of 2009. The incidences and level of deoxynivalenol (DON), nivalenol (NIV), and zearalenone (ZEA) were 34%, 0.89 mg/kg, 84.9%, 1.86 mg/kg, and 10.3%, 0.06 mg/kg respectively. The both levels and incidences of NIV were found to be highest in barley, whereas the level of DON was found to be highest in wheat. Incidences of DON and NIV and the level of NIV in the samples from southern regions of Korea were higher than those from central region, whereas the level of DON from central region was higher than that from southern regions. This is the first paper demonstrating regional difference in natural occurrence of DON and NIV in wheat and barley.

• Safe Food
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• v.2 no.4
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• pp.13-16
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• 2007