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http://dx.doi.org/10.5333/KGFS.2022.42.4.229

The Effect of Single and Mixed Microbial Inoculation on the in situ Fiber Digestibility and Silage of Rice Straw Contaminated Mycotoxins  

Ha Guyn Sung (Department of Animal Science, Sangji University)
Publication Information
Journal of The Korean Society of Grassland and Forage Science / v.42, no.4, 2022 , pp. 229-236 More about this Journal
Abstract
This study was conducted to evaluate the efficacy of adding the microbial inoculants to silage for reducing mycotoxins in rice straw silage. When a single agent of L. plantarum and a mixed agent of L. plantarum and S. cerevisiae were added in rice straw silage contaminated mycotoxins, it had an effect on silage fermentation and fiber degradation as well as mycotoxin reduction. Among the mycotoxins, only ochratoxin A and zearalenone were found in the test sample. Ochratoxin A and zearalenone showed a decreasing trend with the addition of silage inoculants compared to the control groups (38.11±2.22 and 633.67±50.30 ㎍/kg), and there was a significant difference at the mixed agents; 27.78±2.28 and 392.72±25.04 ㎍/kg, respectively (p<0.05). The pH was lower in the single agent and the mixed agent compared to the control (p<0.05). The concentration of lactic acid was higher in the single agent (11.73±0.31 mM) than in the control group (8.18±0.93 mM), and the highest concentration was 16.01±0.88 mM in the mixed agent (p<0.05). Acetic acid and propionic acid were found to be significantly lowered with the addition of silage inoculants (p<0.05). Total VFA was also lower at the addition of silage inoculants than the control group (p<0.05). The rumen in situ dry matter degradation of NDF and ADF was maintained at the highest levels of the mixed agent during the culture period, followed by the single agent and the control group at the lowest level. NDF and ADF degradation showed a significant difference at all time points after 12 and 24 hours of culture, respectively (p<0.05). The study results showed that the silage inoculants had the positive effects on quality increasing of rice straw silage; fermentative charateristics, fiber degradation and mycotoxins reduction. Ochratoxin A and zearalenone were greater reduction by adding bacterial inoculants of silage. Therefore it is considered that L. plantarum and S. cerevisiae will improve the quality and stability with remediation of mycotoxin in silage.
Keywords
L. plantarum; S. cerevisiae Mycotoxin; Rice straw; Silage;
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1 Ahlberg, S.H., Joutsjoki, V. and Korhonena, H.J. 2015. Potential of lactic acid bacteria in aflatoxin risk mitigation. International Journal of Food Microbiology. 207:87-102. doi:10.1016/j.ijfoodmicro.2015.04.042    DOI
2 Akiyama, H., Goda, Y., Tanaka, T. and Toyoda, M. 2001. Determination of aflatoxins B1, B2, G1 and G2 in spices using a multifunctional column clean-up. Journal of Chromatography. 932:153-157. doi:10.1016/s0021-9673(01)01211-0    DOI
3 Caloni, F., Spotti, M.. Auerbach, H., Op den Camp, H., Fonk, G.J. and Pompa, G. 2000. In vitro metabolism of fumonisin B1 by ruminal microflora. Veterinary Research Communications. 24:379-387. doi:10.1023/a:1006422200226    DOI
4 Cho, S., Kwon, C.H. and Kim, E.J. 2014. Effects of bacterial inoculants and organic acids on silage quality: Meta-analysis. Journal of the Korean Society of Grassland and Forage Science. 34:94-102. doi:10.5333/KGFS.2014.34.2.94    DOI
5 Choi, K.C., Ilavenil, S., Arasu, M.V., Park, H.S. and Kim, W.H. 2015. Effect of addition of chlorella and lactic acid bacteria on nutritive value and fermentation quality of fresh rice straw silage. Journal of the Korean Society of Grassland and Forage Science. 35:159-165. doi:10.5333/KGFS.2015.35.2.159    DOI
6 Fink-Gremmels, J. 2008. The role of mycotoxins in the health and perforemance of dairy cows. Veterinary Journal. 176:84-92. doi:10.1016/j.tvjl.2007.12.034    DOI
7 Fussel, R.J. and McCalley, D.V. 1987. Determination of volatile fatty acids(C2-C5) and lactic acid in silage by gas chromatography. Analyst. 112:1213-1216. doi:10.1039/an9871201213    DOI
8 Goering, H. and Van Soest, P.J. 1970. Forage fiber analysis. Agric. Handb. 379. US Department of Agriculture, Washington, DC.
9 Gourama, H. and Bullerman, L.B. 1995. Inhibition of growth and aflatoxin production of Aspergillus flavus by Lactobacillus species. Journal of Food Protection. 58:1249-1256. doi:10.4315/0362-028X-58.11.1249    DOI
10 Hammond, B.G., Campbell, K.W., Pilcher, C.D., Degooyer, T.A., Robinson, A.E., McMillen, B.I., Spangler, S.M., Riordan, S.G., Rice, L.G. and Richard, J.L. 2004. Lower fumonisin mycotoxin levels in the grain of Bt corn grown in the Unite State in 2000-2002. Journal of Agricultural and Food Chemistry. 52:1390-1397. doi:10.1021/jf030441c    DOI
11 Keady, T.W.J. and Steen, R.W.J. 1994. Effects of treating loe dry-matter grass with a bacterial inoculant on the intake and performance of beef cattle and studies on its mode of action. Grass Forage Science. 49:438-446. doi:10.1111/j.1365-2494.1995.tb02317.x    DOI
12 Kim, J.G., Ham, J.S., Chung, E.S., Yoon, S.H., Kim, M.J., Park, H.S,, Lim, Y.C. and Seo, S. 2008. Evaluation of fermentation ability of microbes for whole crop rice silage inoculant. Journal of The Korean Society of Grassland Science. 28:229-236. doi:o10.5333/KGFS.2008.28.3.229    DOI
13 Kim, J.G., Ham, J.S., Li, Y.W., Park, H.S., Huh, C.S. and Park, B.C. 2017. Development of a new lactic acid bacterial inoculant for fresh rice straw silage. Asian-Australasian Journal of Animal Sciences. 30:950-956. doi:10.5713/ajas.17.0287    DOI
14 Lee, S.M., Guan, L.L., Eun, J.S., Kim, C.H., Lee, S.J., Kim, E.T. and Lee, S.S. 2014. The effect of anaerobic fungal inoculation on the fermentation characteristics of rice straw silages. Journal of Applied Microbiology. 118:565-573. doi:10.1111/jam.12724    DOI
15 Li, J., Shen, Y. and Cai1, Y. 2010. Improvement of fermentation quality of rice straw silage by application of a bacterial inoculant and glucose. Asian-Australasian Journal of Animal Sciences. 23:901-906. doi:10.5713/ajas.2010.90403    DOI
16 Li, S., Marquardt, R.R., Frohlich, A.A., Vitti, T.G. and Crow, G. 1997. Pharmacokinetics of ochratoxin A and its metabolites in rats. Toxicology and Applied Pharmacology. 145:82-90. doi:10.1006/taap.1997.8155    DOI
17 Mari, L.J., Schmidt, R.J., Nussio, L.G., Hallada, C.M. and Kung, L. 2009. Short communication: An evaluation of the effectiveness of Lactobacillus buchneri 40788 to alter fermentation and improve the aerobic stability of corn silage in farm silos. Journal of Dairy Science. 92:1174-1176. doi:10.3168/jds.2008-1700    DOI
18 Liu, M., Zhao, L., Gong, G., Zhang, L., Shi, L., Dai, J., Han, Y., Wu, Y., Khalil, M.M. and Sun, L. 2022. Invited review: Remediation strategies for mycotoxin control in feed. Journal of Animal Science and Biotechnology. 13:1-16. doi:10.1186/s40104-021-00661-4    DOI
19 Madrid, J., Martínez-Teruel, A., Hernández, F. and Megías, M.D. 1999. A comparative study on the determination of lactic acid in silage juice by colorimetric, high-performance liquid chromatography and enzymatic methods. Journal of the Science of Food and Agriculture. 79:1722-1726. doi:10.1002/(SICI)1097-0010 (199909)79:12<1722::AID-JSFA427>3.0.CO;2-S    DOI
20 Manni, K., Ramo, S., Franco, M., Rinne, M. and Huuskonen, A. 2022. Occurrence of mycotoxins in grass and whole-crop cereal cilages-A farm survey. Agriculture. 398:1-15. doi:10.3390/agriculture12030398    DOI
21 Mateo, J.J., Liorens, A., Mateo, R. and Jimenez, M. 2001. Critical study of and improvements in chromatographic methods for the analysis of type B trichothecenes. Journal of Chromatography. 918:99-112.    DOI
22 Mayne, C.S. 1990. An evaluation of an inoculant of Lactobacillus plantrum as an additive for grass silage for dairy cattle. Animal Production. 51:1-3. doi:10.1016/S0021-9673(01)00704-X    DOI
23 Megias, M.D, Martinez-Teruel, A., Gallego, J.A. and Nunfez, J.M. 1993. Chemical changes during ensiling of orange peel. Animal Feed Science and Technology. 43:269-274. doi:10.1016/0377-8401(93)90082-U    DOI
24 Niderkorn, V., Morgavi, D.P., Pujos, E., Tissandier, A. and Boudra, H. 2007. Screening of fermentative bacteria for their ability to bind and biotransform deoxynivalenol, zearalenone and fumonisins in an in vitro simulated corn silage model. Food Additives and Contaminants. 24:406-415. doi:10.1080/02652030601101110    DOI
25 Mehrez, A.A. and Orskov, E.I. 1977. A study of the artificial bac technique for determining the digestibility of feeds in the rumen. The Journal of Agricultural Science. 88:645-652. doi:10.1071/AR98169    DOI
26 Mohd-Setapar, S.H., Abd-Talib, N. and Aziz, R. 2012. Review on crucial parameters of silage quality. APCBEE Procedia. 3:99-103. doi:10.1016/j.apcbee.2012.06.053    DOI
27 Niderkorn, V., Boudra, H. and Morgavi, D. 2006. Binding of Fusarium mycotoxꠓins by fermentative bacteria in vitro. Journal of Applied Microbiology. 101:849-856. doi:10.1111/j.1365-2672.2006.02958.x    DOI
28 Ogunade, I.M., Martinez-Tuppia, C., Queiroz, O.C.M., Jiang, Y., Drouin, P., Wu, F., Vyas, D. and Adesogan, A.T. 2018. Silage review: Mycotoxins in silage: Occurrence, effects, prevention, and mitigation. Journal of Dairy Science. 101:4034-4059. doi:10.3168/jds.2017-13788    DOI
29 Oladosu, Y., Mohd, Y., Rafii, L., Abdullah, N., Magaji, U., Hussin, G., Ramli, A. and Miah, G. 2016. Fermentation quality and additives: A case of rice straw silage. BioMed Research International. 7985167. doi:10.1155/2016/7985167    DOI
30 Oliveira, A.S., Weinberg, Z.G., Ogunade, I.M., Cervantes, A.A.P., Arriola, K.G., Jiang, Y., Kim, D.H., Li, X., Goncalves, M.C.M., Vyas, D. and Adesogan, A.T. 2017. Meta-analysis of effects of homofermentative and facultative heterofermentative lactic acid bacteria inoculation on silage fermentation and aerobic stability and the performance of dairy cows. Journal of Dairy Science. 100:4587-4603.1176. doi:10.3168/jds.2016-11815    DOI
31 Rodrigues, I.A. 2014. A review on the effects of mycotoxins in dairy ruminants. Animal Production Science. 54:1155-1165. doi:10.1071/AN13492    DOI
32 Pahlow, G., Muck, R.E., Driehuis, F., Elferink, S.J.W.H.O. and Spoelstra, S.F. 2003. Microbiology of ensiling. In: D.R. Buxton, R.E. Muck and J.H. Harrison (Eds.), Silage science and technology. Madison, WI: Agron. Monogr. ASA. CSSA and SSSA. pp. 31-94. 
33 Piotrowska, M. and Zakowska, Z. 2000. The biodegradation of ochratoxin A in food products by lactic acid bacteria and baker's yeast. Food Biotechnology. 17:307-310. doi:10.1016/S0921-0423(00)80085-4    DOI
34 Ranjit, N.K. and Kung Jr, L. 2000. The effect of Lactobacillus plantarum and L. buchneri on the fermentation and aerobic stability of corn silage. Journal of Dairy Science. 83:526-535. doi:10.3168/jds.S0022-0302(00)74912-5    DOI
35 Rooke, J.A., Maya, F.M., Arnold, J.A. and Amstrong, D.G. 1988. The chemical composition and nutritive value of grass silage prepared with no additive or with the application of additives containing either Lactobacillus plantarum of formic acid. Grass and Forage Science. 43:87-95. First Published: March 1988. doi:10.1111/j.1365-2494.1988.tb02144.x    DOI
36 SPSS. 2017. Statistical package for the social sciences. IBM SPSS Statistics 25.0. IBM Now York USA. 
37 Sung, H.G. 2013. The studies on real condition of mycotoxin contamination in the fields before harvest and by the storage of rice straw using as roughage in Korea. Journal of the Korean Society of Grassland. 33(1):21-29. doi:10.5333/KGFS.2013.33.1.21    DOI
38 Sung, H.G., Lee, J.K. and Seo, S. 2011. Studies on fungi contamination and mycotoxins of rice straw round bale silage. Journal of The Korean Society of Grassland Science. 31:451-462. doi:10.5333/KGFS.2011.31.4.451    DOI
39 Uyeno, Y., Chao, W., Jayanegara, A., Kondo, M., Ban-Tokuda, T. and Matsui, H. 2016. Increase in rumen fibrolytic bacteria and the improvement of fiber degradability of ensiled total mixed ration ssessed by in vitro rumen culture. Advances in Animal and Veterinary Sciences. 4:183-186. doi:10.14737/journal.aavs/2016/4.4.183.186    DOI
40 Upadhaya, S.D., Park, M. and Ha, J.K. 2010, Mycotoxins and their Biotrans-formation in the rumenn: A review. Asian-Australasian Journal of Animal Sciences. 23:1250-1260. doi:10.5713/ajas. 2010.r.06    DOI
41 Van Soest, P.J., Robertson, J.B. and Lewis, B.A. 1991. Carbohydrate methodology, metabolism, and nutritional implications in dairy cattle: Methods for dietary fibre, neutral detergent fibre, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science. 74:3583-3597.    DOI
42 Visconti, A. and Pascales, M. 1998. Determination of zearalenone in corn by means of immunoaffinity clean-up and high-performance liquid chromatography with fluorescence detection. Journal of Chromatography A. 31:133-140. doi:10.1016/s0021-9673(98)00296-9    DOI
43 Wambacq, E., Vanhoutte, I., Audenaert, K., Gelder, L.D. and Haesaert, G. 2016. Occurrence, prevention and remediation of toxigenic fungi and mycotoxins in silage: A review. Journal of the Science of Food and Agriculture. 96:2284-2302. doi:10.1002/jsfa.7565    DOI
44 Weinberg, Z., Muck, R. and Weimer, P. 2003. The survival of silage inoculant lactic acid bacteria in rumen fluid. Journal of Applied Microbiology. 94:1066-1071. doi:10.1046/j.1365-2672.2003.01942.x    DOI
45 Weinberg, Z.G. and Muck, R.E. 1996. New trends and opportunities in the development and use of inoculants for silage. FEMS Microbiology Reviews. 19:53-68. doi:10.1111/J.1574-6976.1996.TB00253.X    DOI
46 Yanga, C., Songa, G. and Limb, W. 2020. Effects of mycotoxin-contaminated feed on farm animals. Journal of Hazardous Materials. 389:1-10. doi:10.1016/j.jhazmat.2020.122087    DOI