• Title/Summary/Keyword: 골형성

Search Result 835, Processing Time 0.026 seconds

The Early Life History of the Rockfish, Sebastes inermis -2. Morphological and Skeletal Development of Larvae and Juveniles- (볼락, Sebastes inermis의 초기생활사에 관한 연구 -2. 산출 자치어의 외부형태 및 골격발달-)

  • KIM Yong-Uk;HAN Kyeong-Ho;BYUN Sun-kyu
    • Korean Journal of Fisheries and Aquatic Sciences
    • /
    • v.26 no.5
    • /
    • pp.465-476
    • /
    • 1993
  • Rockfish, Sebastes inermis is a ovoviviparous fish distributed around the coastal waters of Korea and Japan. Naturally beared larvae obtained from female fish kept in the aquarium were reared for three months in the laboratory. The naturally healed larvae were 6.05mm in average total length(TL) with $8+18{\sim}19=26{\sim}27$ myomeres. Melanophores were distributed on the top of head, around the intestine tube and on the dorsal and ventral margins of the tail, but none on the pectoral fins. The larva(7.1mm in TL) consumed all the yolk and oil globule, in 7 days after bearing, and jaw bones were more rapidly ossified than vertebrae and cranium. In 21 days after bearing, the larvae averaged 8.50mm in TL and the caudal notochord flex $45^{\circ}$. Fin development in S. inermis larvae proceeds in the following sequence: caudal, pectoral, dorsal, anal and ventral fin. Individuals $17.6{\sim}20.8mm$ in TL($65{\sim}69$ days after bearing) are regarded as the juvenile stage. All fins bones was nearly completed in this stage.

  • PDF

Success and failure of endodontic microsurgery (미세 치근단 수술의 성공과 실패)

  • Song, Min-Ju;Kim, Eui-Seong
    • Restorative Dentistry and Endodontics
    • /
    • v.36 no.6
    • /
    • pp.465-476
    • /
    • 2011
  • In current endodontic practice, introduction of operating microscope, ultrasonic instruments, and microinstruments has induced a big change in the field of surgical retreatment. In this study, we aimed to offer key steps of endodontic microsurgery procedure compared with traditional root-end surgery, and to evaluate factors influencing success and failure based on published articles. Endodontic microsurgery is a surgical procedure performed with the aid of a microscope, ultrasonic instruments and modern microsurgical instruments. The microscope provides magnification and illumination - essential for identifying minute details of the apical anatomy. Ultrasonic instruments facilitate the precise root-end preparation that is within the anatomical space of the canal. Modern endodontics can therefore be performed with precision and predictability, thus eliminating the disadvantages inherent in traditional periapical surgery such as large osteotomy, beveled apicoectomy, inaccurate root-end preparation and the inability to observe isthmus. Factors influencing the outcomes of endodontic microsurgery may be diverse, but standardization of procedures can minimize its range. Among patient and tooth-related factors, periodontal status and tooth position are known to be prognostic, but there are only few articles concerning this matter. High-evidence randomized clinical trials or prospective cohort studies are needed to confirm these findings.

Effects of Ipriflavone on bone remodeling in the rat calvarial cell (백서 두개관세포에서 Ipriflavone이 골조직 개조에 미치는 영향)

  • Lee, Yong-Seung;Kim, Young-Jun;Lee, Ki-Heon;Hwang, Hyeon-Shik
    • The korean journal of orthodontics
    • /
    • v.35 no.4 s.111
    • /
    • pp.275-285
    • /
    • 2005
  • Ipriflavone (isoprofoxyisoflavone), a synthetic derivative from soy isoflavone diazein, has been shown to inhibit bone resorption and perhaps stimulate bone formation This study was performed to examine the effects of ipriflavone on the proliferation and bone remodeling in rat calvarial cells in vitro The rat calvarial cells were isolated from fetus aged 20 to 21 days and cultured In BGJb media The graded concentration of ipriflavone $(10^{-9}\;10^{-5}M)$ was administered into cultured cells. When the cell proliferation was estimated through the measurement of MTT assay, there was no increase in cellular proliferation of the rat calvarial cell at any ipriflavone concentration. The cellular activity was evaluated through the formation of mineralized nodules stained by alizarin red. The formation of mineralized nodules significantly increased at concentrations of $10^{-8}M,\;10^{-7}M\;and\;10^{-6}M$ ipriflavone. Reverse transcription-polymerase chain reaction analyses (RT-PCR) were done at 7 and 14 days after culture to detect the expression of Bone Sialoprotein (BSP), Type I Collagen (COL I) and Osteocalcin(OCN) As a result, the expressions of BSP and COL I increased on the 7th day of culture and the expression of OCN increased on the 14th day of culture. These results indicate that ipriflavone facilitates the bone remodeling process bvy promoting rat calvarial cell differentiation aid stimulating mineralization through increased expression of extracellular matrix genes. such as BSP. COL I and OCN.

Effect of Pyrroloquinoline Quinone on Osteoclast Generation and Activity (Pyrroloquinoline quinone이 파골세포의 생성 및 활성에 미치는 영향)

  • Ko, Seon-Yle;Han, Dong-Ho;Kim, Jung-Keun
    • Journal of Oral Medicine and Pain
    • /
    • v.30 no.3
    • /
    • pp.329-336
    • /
    • 2005
  • We examined the effect of PQQ, as a scavenger of superoxide, on osteoclast-like cell formation and on mature osteoclast function. To determine whether PQQ scavenges the superoxide, nitroblue tetrazolium (NBT) staining, which is a method to detect superoxide, was performed on HD-11 cells which are a chick myelomonocytic cell line having tartrate-resistant acid phosphatase (TRAP) activity in response to 1,25-dihydroxyvitamin $D_3\;[1,25(OH)_2D_3]$. Histochemical study of TRAP was also performed on HD-11 cells. PQQ inhibited the TRAP-positive multinucleated cell formation of chicken bone marrow cells was also examined. The addition of 20 ${\mu}M$ PQQ inhibited the formation of TRAP-positive multinucleated cell. When chicken osteoclasts were cultured on dentin slices, treatment of 20 ${\mu}M$ PQQ resulted in a significant decrease in dentin resorption by osteoclasts in terms of total resorption area and number of resorption pits. The present data suggest that PQQ, possibly as a scavenger of superoxide ion, inhibits the osteoclastic differentiation and bone resorption.

Antiinflammatory Effects of Tetracycline-loaded Biodegradable Membranes in Experimental Periodontitis of Beagle Dogs (실험적으로 치주염을 유발한 비글견에서 테트라싸이클린 함유 생분해성 차폐막의 항염효과)

  • Jun, Hyung-Sik;Seol, Yang-Jo;Park, Yoon-Jeong;Lee, Yong-Moo;Ku, Young;Rhyu, In-Cheul;Lee, Seung-Jin;Han, Soo-Boo;Choi, Sang-Mook;Kwon, Soo-Kyoung;Chung, Chong-Pyoung
    • Journal of Periodontal and Implant Science
    • /
    • v.30 no.3
    • /
    • pp.583-599
    • /
    • 2000
  • 조직유도재생술 과정에 사용된 차폐막의 술 후 오염 혹은 시술부위의 감염으로 조직재생유도가 제대로 이루어지지 않는 경우가 많이 있다. 테트라싸이클린은 넓은 범위의 치주 원인균에 효과적이고, 중성구 교원분해효소를 억제 함으로써 결합조직파괴를 억제하기 때문에 전신적 혹은 국소적으로 치주질환 치료에 널리 이용되어져왔다. 이번 연구의 목적은 비글견에 실험적으로 치주염을 유발시킨 후 테트라싸이클린 함유 차폐막을 이용하여 조직유도재생술을 하고서 테트라싸이클린의 유리반응을 관찰하는 것과 테트라싸이클린에 의한 항염, 항균효과를 알아보는 것이다. 실험 2-3개월 전에 비글견의 구강에서 치조골 결손부를 형성하여 치주염을 유발시켰다. 결손부 형성 2-3개월 후 실험군으로 테트라싸이클린 함유 차폐막으로 조직유도재생술을 하였고, 대조군으로 테트라 싸이클린이 함유되지 않은 차폐막으로 조직유도재생술을 하였고, 음성대조군으로 치은판막술만을 하였다. 시술 전과 시술 후 1, 2, 4주 간격으로 치은지수, 치태지수, 치은열구액의 양 및 혐기성 세균과 호기성 세균의 군락수를 측정하였다. 술 후, 1, 3, 5, 7, 및 14일 간격으로 유리된 테트라싸이클린의 농도를 측정하였다. 테트라싸이클린 함유 차폐막은 임상적 치은지수는 술 후 2, 4주째, 치태지수는 1, 2, 4주째 유의하게 감소하였다.(p<0.05) 1주에서 혐기성 및 호기성 세균집락수는 테트라싸이클린 함유 차폐막을 사용한 군이 약물이 함유되지 않은 차폐막보다 유의성있게 적었다.(p<0.001) 테트라싸이클린 함유 차폐막은 처음 1일은 높은 농도로 유리되었고 그 후 1주일 동안 일정하게 MIC 이상으로 유리되었다. 하지만 14일째는 유리가 관찰되지 않았다. 본 연구 결과, 테트라싸이클린 함유 생분해성 차폐막은 1주이상 약제가 MIC 이상 유리되었고, 유리된 테트라싸이클린에 의한 항염, 항균작용이 있어 치주조직 재생유도술시에 초기 치유가 잘 진행되도록 하는 효과가 있을 것이다.

  • PDF

A study of $TGF-\beta$ Expression Patterns In Cleft Palate Formed Rats Induced by BAPN (BAPN으로 유도한 구개열 백서에서 $TGF-\beta$ 발현 양상에 대한 연구)

  • Tae, Ki-Chul;Kim, En-Chel
    • The korean journal of orthodontics
    • /
    • v.31 no.6 s.89
    • /
    • pp.579-587
    • /
    • 2001
  • Cleft palate has been studied with epidemiologic and molecular methods, and many etiologic factors have been examined closely Among the research methods, biologic molecule research has been the most important method for cleft palate formation study The $TGF-\beta$ played an important role in cell migration, epithelial-mesenchymal transdifferentiation, extracellular matrix synthesis and deposition. But there was not much research on the correlation cleft palate induced by beta-aminonitroproprionitrile(BAPN) and $TGF-\beta$ expression. The purpose of the present study was to examine how $TGF-\beta$ is expressed in cleft palate rats. 4 Timed-pregnant Sprague-Dawley rats were obtained on the 10th gestation day. On the 13th day of gestation, BAPN-monofumarate salts (${(C_3H_6N_2)}_2{\cdot}C_4H_4O_4$) were individually, ovally administered to 3 pregnant rats at a ratio of 1g/kg body weight. And 4 pregnant rats were sacrificed on day 20 post coitus (p.c.). The $TGF-\beta$ expression in the cleft formed rats fetuses showed the following patterns : 1. Osteoblast and mesenchymal cells of the cleft pa)ate rats were of low expression compared with those of the control rats. 2. The cleft palate rats didn't show uy difference in the $TGF-\beta$ expression of osteocyte item the control rats. 3. In western blot analysis, the thickness of band of $TGF-\beta$ in the cleft palate rats was thinner and more diluted than that of the control rats.

  • PDF

A Study on the Preference on Protein Rich Foods in Kindergarten Children in Gyeong Nam Area (단백질(蛋白質) 급원식품(給源食品)을 기피(忌避)하는 일부(一部) 유치원(幼稚園) 아동(兒童)의 식이형성(食餌形成)과 그 요인(要因))

  • Yoon, Hyun-Sook
    • Journal of Nutrition and Health
    • /
    • v.17 no.1
    • /
    • pp.3-9
    • /
    • 1984
  • Preference on protein rich foods of 103 children, aged 4 to 6, of kindergarten located in Masan Jin-hea, Chang-won cities, was conducted April 25 to May 4, 1983. The results are summarized as follows ; 1) Seventy six percent of the subjects were from families having two three children, without grandmothers in the home About 43% of mother had completed high school, 37% had completed middle school. Mothers' ages ranged from 26 to 50 years, with 57.3% in the 31-40 age bracket. About 63% of the fathers were office and government employees, while 77.6% of mothers were unemployed. 2) Over 50% of the children disliked or refused pork, thick beef soup, liver, soybean, oyster, clam, anchovy, croaker, mackerel, loach, hairtail, porgy, flatfish, a walleye, pollack, a dried walleye pollack, a sciaenoid fish. Taste bad, the characteristic fragrance and lack of experience were the main reasons why the children refused these foods. Over 33% of the children were unexperienced liver, ribs of beef. the small intestine of cattle, a loach, an eel. Over 50% liked milk, egg, dried filefish, sausage, a cattlefish, beef, chicken, a crab, shrimp, bean curd. 3) Most of children hoped that their daily meals should be made in prettier, more sabory, and various ways. Mothers also hoped to have practical knowledge of nutrition and its influence on our body.

  • PDF

Antimicrobial Effect of Mouthwash against Streptococcus mutans by Visual Staining Method (시각적 염색 방법을 이용한 마우스워시의 구강균에 대한 항균효과 확인)

  • Park, Taehun;Cho, Jeong Hun;Sung, Youngeun;Cho, Jun-Cheol;Shin, Kyeho
    • Journal of the Society of Cosmetic Scientists of Korea
    • /
    • v.40 no.2
    • /
    • pp.187-193
    • /
    • 2014
  • Dental caries are one of the most common oral diseases and Streptococcus mutans (S. mutans) plays an important role in the initiation and progression of dental caries. Oral malodor is primarily the result of microbial metabolism such as Porphyromonas gingivalis (P. gingivalis) that produce volatile sulfur compounds (VSCs), causing oral malodor. Prevotella intermedia (P. intermedia) is known as typical periodontopathic bacteria, and periodontal disease is chronic inflammatory disease that leads to damage of gingival connective tissue and alveolar bone, eventually loss of teeth. In this study, we investigated antimicrobial effect of mouthwash containing cetylpyridinium chloride (CPC), sodium fluoride (NaF), green tea water extract and pine needles water extract against cariogenic and periodontopathic bacteria sucn as S. mutans, P. gingivalis and P. intermedia. As a result, the reduction ratios of S. mutans and P. gingivalis were 4.00 Log and 4.68 Log reduction for 30 s, and P. intermedia were 2.40 Log reduction for 30 s and 2.70 Log reduction for 60 s. Dentocult SM Strip mutans (SM Strip) provides easy detection of visual data showing a significant inhibition on S. mutans. In conclusion, we expected that mouthwash containing CPC, NaF, green tea water extract and pine needles water extract could help preventing the dental disease like dental caries and oral malodor.

TREATMENT OF HORIZONTAL AND VERTICAL ROOT FRACTURE IN IMMATURE PERMANENT TEETH - A CASE REPORT (미성숙 치근의 수직 파절과 수평 파절의 치험례)

  • Song, Seung-Ho;Park, Ho-Won
    • Journal of the korean academy of Pediatric Dentistry
    • /
    • v.31 no.1
    • /
    • pp.92-97
    • /
    • 2004
  • Traumatic injuries in the young peranent dentition are common, but root fractures, defined as fractures in volving dentin, cementum and pulp, are relatively uncommon. Case 1 is a 9-year-old boy who had a horizontal root fracture of his maxillary right central incisor in the apical third. Root canal therapy was performed in coronal segment and calcium hydroxide therapy was initiated. Six months after treatment, a periapical radiograph showed calcific tissue formation and normal root development. 1 year and 3 months later, the canal was permanently obturated with gutta-percha. Case 2 is a 7-year-old girl who had a vertical root fracture of her maxillary right central incisor. Fractured tooth was intentionally extracted atraumatically, and then the separated fragments are bonded with resin cement. the restored tooth was replanted into the original socket. Recalls up to 8 months showed normal mobility and no periapical pathosis. In these cases, we performed conservative treatment. Clinical and radiographic examination showed no pathosis or abnormality of the teeth and periodontal tissue.

  • PDF

Comparative Gene-Expression Analysis of Periodontal Ligament and Dental Pulp in the Human Permanent Teeth (사람 영구치에서 치주인대 및 치수 조직의 유전자 발현에 대한 비교 연구)

  • Lee, Suk Woo;Jeon, Mijeong;Lee, Hyo-Seol;Song, Je Seon;Son, Heung-Kyu;Choi, Hyung-Jun;Jung, Han-Sung;Moon, Seok-Jun;Park, Wonse;Kim, Seong-Oh
    • Journal of the korean academy of Pediatric Dentistry
    • /
    • v.43 no.2
    • /
    • pp.166-175
    • /
    • 2016
  • There is no genetic activity information with the functions of dental pulp and periodontal ligament in human. The purpose of this study was to identify the gene-expression profiles of, and the molecular biological differences between periodontal ligament and dental pulp obtained from human permanent teeth. cDNA microarray analysis identified 347 genes with a fourfold or greater difference in expression level between the two tissue types 83 and 264, of which were more plentiful in periodontal ligament and dental pulp, respectively. Periodontal ligament exhibited strong expression of genes related to collagen synthesis (FAP), collagen degradation (MMP3, MMP9, and MMP13), and bone development and remodeling (SSP1, BMP3, ACP5, CTSK, and PTHLH). Pulp exhibited strong expression of genes associated with calcium ions (CALB1, SCIN, and CDH12) and the mineralization and formation of enamel and dentin (SPARC/SPOCK3, PHEX, AMBN, and DSPP). Among these genes, SPP1, SPARC/SPOCK3, AMBN, and DSPP were well known in dental research. However, the other genes are the newly found and it may help to find a good source of regenerative therapy if further study is performed.