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Effect of Pyrroloquinoline Quinone on Osteoclast Generation and Activity  

Ko, Seon-Yle (Department of Oral Biochemistry, School of dentistry, Dankook University)
Han, Dong-Ho (Department of Oral Biochemistry, School of dentistry, Dankook University)
Kim, Jung-Keun (Department of Oral Biochemistry, School of dentistry, Dankook University)
Publication Information
Journal of Oral Medicine and Pain / v.30, no.3, 2005 , pp. 329-336 More about this Journal
Abstract
We examined the effect of PQQ, as a scavenger of superoxide, on osteoclast-like cell formation and on mature osteoclast function. To determine whether PQQ scavenges the superoxide, nitroblue tetrazolium (NBT) staining, which is a method to detect superoxide, was performed on HD-11 cells which are a chick myelomonocytic cell line having tartrate-resistant acid phosphatase (TRAP) activity in response to 1,25-dihydroxyvitamin $D_3\;[1,25(OH)_2D_3]$. Histochemical study of TRAP was also performed on HD-11 cells. PQQ inhibited the TRAP-positive multinucleated cell formation of chicken bone marrow cells was also examined. The addition of 20 ${\mu}M$ PQQ inhibited the formation of TRAP-positive multinucleated cell. When chicken osteoclasts were cultured on dentin slices, treatment of 20 ${\mu}M$ PQQ resulted in a significant decrease in dentin resorption by osteoclasts in terms of total resorption area and number of resorption pits. The present data suggest that PQQ, possibly as a scavenger of superoxide ion, inhibits the osteoclastic differentiation and bone resorption.
Keywords
Osteoclast; Superoxide; Pyrroloquinoline quinone; TRAP;
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