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Effects of Ipriflavone on bone remodeling in the rat calvarial cell  

Lee, Yong-Seung (Department of Orthodontics, School of Dentistry, Chonnam National University)
Kim, Young-Jun (Department of Periodontology, School of Dentistry, Dental Science Research Institue, Chonnam National University)
Lee, Ki-Heon (Department of Orthodontics, School of Dentistry, Dental Science Research Institute, Chonnam National University)
Hwang, Hyeon-Shik (Department of Orthodontics, School of Dentistry, Dental Science Research Institute, Chonnam National University)
Publication Information
The korean journal of orthodontics / v.35, no.4, 2005 , pp. 275-285 More about this Journal
Abstract
Ipriflavone (isoprofoxyisoflavone), a synthetic derivative from soy isoflavone diazein, has been shown to inhibit bone resorption and perhaps stimulate bone formation This study was performed to examine the effects of ipriflavone on the proliferation and bone remodeling in rat calvarial cells in vitro The rat calvarial cells were isolated from fetus aged 20 to 21 days and cultured In BGJb media The graded concentration of ipriflavone $(10^{-9}\;10^{-5}M)$ was administered into cultured cells. When the cell proliferation was estimated through the measurement of MTT assay, there was no increase in cellular proliferation of the rat calvarial cell at any ipriflavone concentration. The cellular activity was evaluated through the formation of mineralized nodules stained by alizarin red. The formation of mineralized nodules significantly increased at concentrations of $10^{-8}M,\;10^{-7}M\;and\;10^{-6}M$ ipriflavone. Reverse transcription-polymerase chain reaction analyses (RT-PCR) were done at 7 and 14 days after culture to detect the expression of Bone Sialoprotein (BSP), Type I Collagen (COL I) and Osteocalcin(OCN) As a result, the expressions of BSP and COL I increased on the 7th day of culture and the expression of OCN increased on the 14th day of culture. These results indicate that ipriflavone facilitates the bone remodeling process bvy promoting rat calvarial cell differentiation aid stimulating mineralization through increased expression of extracellular matrix genes. such as BSP. COL I and OCN.
Keywords
Ipriflavone; Calvarial cell; Bone remodeling;
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