• Journal of Life Science
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• v.28 no.7
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• pp.857-863
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• 2018

This study examined whether the supplementation of Salicornia herbacea L. (SH), a member of the Chenopodiaceae subfamily, affects tissue specific triglyceride (TG) accumulation and the peroxisome proliferator-activated $receptor-{\gamma}$ $coactivator-1{\alpha}$ ($PGC-1{\alpha}$) and peroxisome proliferator-activated $receptor-{\gamma}$ ($PPAR-{\gamma}$) protein expressions of skeletal muscle in rats with a high-fat diet. Sprague-Dawley male rats were randomly divided into three groups: control normal diet group (CD), high-fat diet group (HD), and 5.0% SH supplemented high-fat diet group (SD). The weights of fat tissue of the SD group were reduced by approximately 25%(p<0.01), while the skeletal muscle weight of the SD group increased approximately 5% compared to those in the HD group (p<0.01). The serum and hepatic TG of the SD group decreased approximately 20% compared to those of the HD group (p<0.05). In the protein expression levels in the skeletal muscle, the $PGC-1{\alpha}$ and $PPAR-{\gamma}$ expressions of the SD group were 1.5-folds higher than those of the HD group (p<0.01). From these results, SH supplementation contributes to the improvement of the serum and hepatic TG concentrations, and the $PGC-1{\alpha}$ and $PPAR-{\gamma}$ protein expression levels in the skeletal muscle of fed a high-fat diet. Thus, SH supplementation was effective in reducing fat mass and increasing muscle mass.

• Journal of Life Science
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• v.26 no.7
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• pp.758-763
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• 2016

Fatty acid transporters are key mediators of skeletal muscle lipid metabolism. Several protein groups have been implicated in cellular long-chain fatty acid uptake or oxidation, including fatty acid transporter proteins (FATPs), the plasma membrane fatty acid-binding protein (FABPpm), and the fatty acid translocase (FAT/CD36). FAT/CD36 is highly expressed in skeletal muscle and known to be regulated by various factors such as exercise and hormones. Insulin-like growth factor-I (IGF-I) is a well-known regulator of skeletal muscle cells. However, it has not been studied whether there is any interaction between IGF-I and FAT/CD36 in skeletal muscle cells. In this study, the effects of IGF-I treatment on FAT/CD36 induction were examined. Differentiated C2C12 cells were treated with 20 ng/ml of IGF-I at different time points. Treatment of C2C12 cells with IGF-I resulted in increased FAT/CD36 mRNA and protein expression. After 24 and 48 hr of IGF-I treatment, FAT/CD36 mRNA increased 89% and 24% respectively. The increase of both proteins returned to the control level after 72 hr of IGF-I treatment, suggesting that the FAT/CD36 gene is regulated pretranslationally by IGF-I in skeletal muscle cells. These results suggest that IGF-I can regulate the expression of FAT/CD36 in skeletal muscle cells. In conclusion, IGF-I induces a rapid transcriptional modification of the FAT/CD36 gene in C2C12 skeletal muscle cells and has modulating effects on fatty acid uptake proteins as well as oxidative proteins.

• Journal of Life Science
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• v.29 no.8
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• pp.837-845
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• 2019

The purpose of this study was to analyze the effects of aerobic exercise and resveratrol supplementation on mitochondrial biogenesis in skeletal muscle of high-fat diet-induced obese mice. In this study, 4-wk-old C57BL/6 male mice were divided into four groups, with 10 animals in each group: a normal diet group (NC), high-fat diet group (HC), high-fat diet group with resveratrol supplementation (HRe), and high-fat diet GROUP with exercise (HE). Aerobic exercise was performed on a treadmill for 40~60 min/d at 10~14 m/min, 0% grade, 4 d/wk for 16 wk. Resveratrol (25 mg/kg bodyweight) was administrated once a day, 4 d/wk for 16 wk. There was a significance difference in COX-IV mRNA expression in the NC group versus that in the HC group (p<0.05). The expression of the SIRT-3, PGC-1a, and COX-IV mRNA genes in the HE group increased significantly as compared with the expression of these genes in the HC and HRe groups (p<0.05). These results indicated that high- fat diet-induced obesity did not affect mitochondria biogenesis gene expression in skeletal muscle. In contrast, aerobic exercise training increased the expression of mitochondria biogenesis gene expression in skeletal muscle in high-fat diet-induced obese mice. These findings suggested that aerobic exercise but not resveratrol supplementation had a positive effect on mitochondrial biogenesis in skeletal muscle in high-fat diet-induced obese mice.

• Journal of Digital Convergence
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• v.12 no.3
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• pp.317-323
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• 2014

This research has a purpose of providing base data to prevent musculoskeletal disorders, by analyzing the changes in muscle body due to sling exercises, of the industrial laborers with work-related musculoskeletal disorders. The subjects of this research are 20 volunteers who applied for musculoskeletal treatment exercises in hospitals, from region D industry H. 10 of the 20 subjects were involved in sling exercise program for 12 weeks, and the other 10 subjects were ordered to retain their common practices. We have used the statistics software SPSS ver.18.0 to analyze the data, and have offered a descriptive analysis of the measured variables. To discover the sling exercise effects, we have executed a t-test (paired sample t-test) comparing the before-after relationship of 12-week sling exercise program, and the level of significance were set to a=.05. With these set of research methods and procedures, we have concluded the following. As a result, we have concluded that 12-week sling exercise program does exert influence in changing muscle body of work-related musculoskeletal patients.

• Ceramist
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• v.7 no.1
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• pp.41-46
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• 2004

우리나라에서 노령화가 급속히 진행됨에 따라 근골격계(뼈 및 관절부분)의 기능 장애는 개인의 삶의 질을 심각하게 저하시킬 뿐만 아니라 개인 및 국가 경제에도 큰 손실을 초래하고 있다. 현재 국내에서 사용되고 있는 근 골격계의 재생/재건/재활 관련 의료기기 대부분은 수입에 의존하고 있다. 고가의 수입제품의 사용은 심각한 경제적 손실 뿐 만 아니라 환자의 의료비 부담에 따른 양질의 의료혜택에도 지장을 주고 있다.(중략)

• Journal of the KSME
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• v.53 no.8
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• pp.28-33
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• 2013

이 글에서는 생체 내 근력과 관절 모멘트, 관절 접촉역학 분석에 이용되는 근골격계 생체역학 모델을 소개하고 그 연구 동향에 대해 소개하고자 한다.

• Journal of Life Science
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• v.22 no.1
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• pp.98-109
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• 2012

The properties of lactate dehydrogenase (EC 1.1.1.27, LDH) and expression of monocarboxylate transporters (MCTs) 1, 2, and 4 were studied in tissues from Micropterus salmoides. Native-PAGE revealed that the LDH $A_4$ isozyme was predominantly located in skeletal muscle. The LDH $A_4$, $A_2B_2$, and $B_4$ isozymes were detected in heart, liver, eye, and brain tissues, while eye-specific $C_4$ isozyme was detected in eye tissue. In September, strong LDH $B_4$ isozyme activity was detected in heart tissue. High $A_4$ isozyme activity was noted in all other tissues except heart tissue. However, in November, strong $A_4$ isozyme activity was detected in heart tissue. The LDH/CS (Citrate synthase, EC 4.1.3.7) ratio in skeletal muscle and heart tissues indicated that anaerobic metabolism was high in those tissues. Native-PAGE after immunoprecipitation showed that eye-specific $C_4$ isozyme was more similar to the $A_4$ than the $B_4$ isozyme. The LDH $A_4$ isozyme was purified by affinity chromatography. The molecular weight of subunit A was 37,200. The LDH activity in tissues was consistently 11.05~28.32% due to inhibition by 10 mM pyruvate. The $K_m^{PYR}$ of LDH in eye tissue was very low. The optimum pH for LDH in tissues was pH 7.5~8.0. The LDH $A_4$ isozyme was detected in mitochondria of skeletal muscle, whereas the $B_4$ and $A_2B_2$ isozymes were detected in heart tissue mitochondria. Western blot analysis indicated that MCTs 1, 2, and 4 were located in the plasma membrane and mitochondria of skeletal muscle and heart tissues. The sizes of MCTs 1, 2, and 4 in skeletal muscle were 60, 54~38, and 63 kDa, while those in heart tissue were 57, 54~38, and 55.5 kDa, respectively. In conclusion, M. salmoides appears to use anaerobic metabolism predominantly when adapted to a hypoxic environment. In highly activated skeletal muscle and heart tissue, energy production is controlled by inward and outward flows of pyruvate and lactate through MCTs 1, 2, and 4 in the plasma membrane and mitochondria, with effective adjustment by LDH isozymes.

• 월간산업보건
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• s.337
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• pp.40-50
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• 2016

• Journal of the Korea Convergence Society
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• v.8 no.2
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• pp.43-51
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• 2017

The purpose of this study was to identify correlations between the musculoskeletal symptoms and job stress of occupational therapists at rehabilitation hospitals. To this end, the study conducted a survey on 210 occupational therapists at rehabilitation hospitals located in Seoul and Gyeonggi-do between June 1 and July 30, 2014. The average age of the subjects who participated in this study was $25.4{\pm}3.4$ years old, and their average work period was $2.3{\pm}2.1$ years. The subjects included 151 women (71.9%) and 171 permanent workers (81.4%). Among the subjects, 114 individuals (54.3%) had experienced a musculoskeletal symptom, and their major affected areas included hands/fingers (21%) and legs (18.6%). The percentage of high risk group of job stress was higher in musculoskeletal symptom group. And the proportion of high risk for job stress was higher in females. In particular, males showed significant differences in 'job demands', 'inappropriate reward', 'occupational climate' and females showed significant differences in 'Interpersonal conflict', 'inappropriate reward'(p<0.05).

• The Journal of the Korea Contents Association
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• v.12 no.4
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• pp.273-281
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• 2012

The purpose of this study was to examine about correlation among dental hygienists' work environment, recognition on preventive education of musculoskeletal disease, the work-related musculoskeletal disease, and the work characteristics. The aim is to suggest a management plan for reducing symptom of musculoskeletal disease. The self-administered questionnaire was surveyed and collected from August 1-12 of 2011 targeting 210 dental hygienists Seoul, Gyeonggi, Daegu, Pohang. Except 5 people whose responses are not clear and who suffered accident, the analysis was made on 205 people. The collected data was analyzed by using SPSS WIN 15.0 program. As a result, dental hygienists, the average was the highest with 3.07 in 'between shoulder and neck(right side).' Seeing the pain frequency by body region, the average was the highest with 1.47 in 'ankle/calf(left side).' Seeing the appearance of having trouble in work ability related to pain experience of musculoskeletal disease by body region, the average was indicated to be the highest with 1.84 in 'knee(right side).Therefore, the agencies have to pay more attentions and efforts to ensure safety of musculoskeletal disorders in dental hygienist.