• Proceedings of the KSAR Conference
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• 2001.03a
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• pp.34-34
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• 2001

본 연구의 목적은 한우 난포발달에 있어서 난포액 및 anti-inhibin serum의 생리적 역할을 검토하기 위해 수행하였다. Anti-inhibin serum(AI)은 항원으로서 porcine inhibin-$\alpha$-subunit 19~32의 peptide를 사용하여 adjuvant 용액을 1:3의 비율로 혼합하여 앙고라종 토끼 5두(체중 2.5kg)에게 주 2회 간격으로 접종 후 얻어진 항혈청을 사용하였다. 난포액(bFF; bovine follicular fluid)은 도축장에서 도축되는 한우 난소로부터 직경 1.0cm 이하의 난포로부터 회수하여 스테로이드를 제거하기 위해 10% chacoal so lution(50 mg/$m\ell$, Norrit-A, Fisher Sci., USA)을 처리하여 45분간 배양후 원심분리후 상층액을 회수하여 실험에 공시하였다. 공시동물은 1산후 정상적으로 발정주기가 반복되는 한우암소 9두를 난소감정후 황체를 확인하여 PGF2$\alpha$제제(lutylase. USA)를 주사하여 발정을 유기한 다음, 난소의 first wave가 시작되는 시기인 배란직후 12시간째부터 4일간 일일 2회 5 $m\ell$씩 총 8회 40 $m\ell$의 AI와 bFF를 각각 경정맥으로 주사하였으며 대조구로서 생리식염수를 주사하였으며 채혈 및 정맥주사를 용이하게 하기 위해 경정맥에 카테타를 설치하여 6시간간격으로 총 200시간까지 채혈하였으며 초음파진단기를 이용하여 난포의 발달을 검토하였다. 채혈후 혈중Inhibin, progesterone 및 Estrad iol-17$\beta$농도의 분석은 RIA 및 ELISA법으로 분석하여 얻어진 결과는 다음과 같다. 혈중 Progesterone농도는 대조구와 AI처리구에서는 배란후 68시간째부터 유의적으로 증가하기 시작하였으나, bFF처리구에서는 배란후 68시간부터 170시간까지 대조구에 비해 유의적으로 낮은 농도를 나타내었다. 이에 반해 혈중 Estradiol-17$\beta$농도는 대조구의 경우 bFF처리구와 비슷한 수준으로 배란후부터 낮은 농도를 유지하였으나, AI 처리구는 배란후 36시간이후부터 108시간까지 유의적으로 높은 수준을 유지하였다가 그 이후 감소되었다. 한편, 혈중 Inhibin농도는 전 구간에서 배란후 84시간까지 불규칙한 농도를 보이다가 bFF처리구에서는 배란후 84시간부터 유의적으로 증가하였다. 배란후 72시간째에 초음파진단기를 이용하여 난소의 난포발달을 조사한 결과 , 대조구와 bFF처리구에 비해 AI처리구에서 발달난포가 유의적으로 많은 것을 확인하였다. 이상과 같은 결과로, Anti-inhibin serum은 한우 자체에서 분비하는 Inhibin을 특이하게 억제하여 Inhibin에 의해 억제되는 FSH분비가 촉진됨으로써 난포발달과 estrogen의 농도가 촉진되는 것으로 사료되어 anti-inhibin serum이 한우의 과배란유기 효과가 있는 것으로 사료된다.

• Childhood Kidney Diseases
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• v.1 no.1
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• pp.60-66
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• 1997

Even though the urinary stones are rare in children, careful observation and monitoring are necessary because the incidence has been increasing. This study is aimed to document the characteristics of urinary stones in children including the symptoms, diagnosis, with or without urinary tract infection, other urinary tract anomaly and treatment. 45 patients under the age of 15 years with urinary stones hospitalized during Jan. 1986 to Jun. 1996 at Severance Hospital were reviewed retrospectively. Patients' mean age was 6.5 years and sex ratio (male : female) was 5.4:1. The most common symptom was gross hematuria. Stones accompanied with urinary tract infection was 46.7%, and stones associated with urinary tract anomaly was 35.6%. Extracorporeal shock wave lithotripsy, percutaneous nephrolithotorny, hydration and diuretics were the treatment modality used. Urinary stone were found to be a significant cause of urinary tract obstruction in children, requiring prompt diagnosis and treatment. Although KUB, IVP, and ultrasonography were commonly used to make the diagnosis, many cases were detected only by ultrasonographic study.

• Journal of Chest Surgery
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• v.40 no.10
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• pp.691-695
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• 2007

Background: The introduction of central venous catheters in 1979 has aided the administration of chemotherapy to oncologic patients. We analyzed the clinical reviews and complications of totally implantable venous catheters in an effort to achieve optimal management. Material and Method: We retrospectively studied 100 cases with totally implantable venous catheter at our hospital and we report the results. Result: 100 totally implantable venous catheters were placed in the right subclavian vein in 74 cases (74%), the left subclavian vein in 21 cases, the right jugular vein in 3 cases, the left jugular vein in 1 case and the right femoral vein in 1 case. The immediate complications were 5 cases in malposition of the catheter and 5 cases of arterial puncture. The late complications were 1 case of subclavian vein thrombosis, which was treated with anticoagulation, and 2 cases of pinch-of syndrome. There were no other early or late complications. Conclusion: The low rate of complications in this study confirms the safety and convenience of using totally implantable venous catheter in patients undergoing prolonged chemotherapy. Yet because Infection, thrombosis, and catheter fracture are the most common long term complications of totally implantable venous catheters, early diagnosis and management of these problems can prevent severe complications.

• Journal of the Korean Applied Science and Technology
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• v.34 no.4
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• pp.839-850
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• 2017

The objective of this study was to investigate the effect of diets with different omega-6 (n-6) and omega-3 (n-3) fatty acid ratios (0, 1.2: 1, 8: 1, 19: 1) on mechanisms of partitioning of in vivo hepatic glycerolipid metabolism in next generation rats. Biomonitoring techniques were used in rats (jugular vein cannula were attached). The range of $^{14}CO_2$ exhaled by glycerolipid metabolism was 7.05-20.17% in the group having diet with n-6/n-3 ratio less than 8:1. It was the lowest in the control group. Total glycerolipid was the highest in the control group. For groups with different n-6/n-3 ratio, it had the following order: 19:1>8:1>1.2:1. Secreted triglyceride was decreased by 36.35%, 20.93%, and 13.72% in 1.2:1,8:1, and 19:1 groups, respectively, compared to that in the control group. The ratio of phospholipid to total glycerolipid was 1.38, 1.29, and 1.17 times higher in 1.2:1, 8:1, and 19:1 groups, respectively, compared to that in the control group. The ratio of $^{14}CO_2$ to total glycerolipid was 1.61, 1.52, and 1.29 times higher in 1.2:1, 8:1, and 19:1 groups, respectively, compared to that in the control group.

• Proceedings of the KSLP Conference
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• 1993.12a
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• pp.20-20
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• 1993

저자들은 1983년 4월부터 1993년 9월까지 10년 6개월간 연세대학교 의과대학 영동세브란스병원 이비인후과에 내원한 종양에 의한 성대마비환자 29례에 대한 임상적 분석을 통하여 다음과 같은 결론을 얻었다. 1) 종양의 종류별로는 폐암이 13예(44.8％)로 가장 많았으며 갑상선암이 8예(27.6％), 신경성종양이 3예(10.4％), 종격동종양이 2예(6.9％), 그 외 경부식도암, 기관지암, 경정맥소체종양이 각각 1예로 나타났다. (중략)

• Journal of Veterinary Clinics
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• v.24 no.3
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• pp.414-418
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• 2007

A 2.8-year-old intact female Beagle dog (weighing 11kg) was referred with the primary complaint of exercise intolerance with occasional syncope. Physical examination revealed irregularly irregular heart rhythm with persistent pulse deficits. The 12-lead surface ECG showed a third degree heart block. Permanent transvenous cardiac pacing with a bipolar implantable pacemaker was performed in the right ventricle. After pacemaker implantation, the dog did not show syncopal episode and is currently able to take a walk with an owner. No side effects associated with permanent pacemaker implantation has been observed to date.

• Journal of Chest Surgery
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• v.29 no.4
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• pp.373-380
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• 1996

Prevention of thromboembolism is the most important task in the development of bioconpatible small caliber artificial vascular graft. In normal vessels, vascular endothelial cells maintain homeosatsis by secreting numerous factors. The aim of this study is to develope a method which Improves biocompatibility of small caliver polyurethane graft using endothelial cell culture technique, and ev luate the efTectiveness of extracelluar matrix for endothelization which was produced by cultured fibroblast. Methods ; Multiporous polyurethane tube of 3 mm diameter, 0.3 mm thickness was manufactured for vascular graft. Three mongrel dogs were intubated and internal jugular veins removed. Extracelluar matrix produced by cultured flbrobast which was obtained from dog's internal jugular vein were coated to the polyurethane graft. Then, endothelial cells extracted from Jugular vein were cultured and fixed on the extracelluar matrix layer of vascular graft. Endothelial cell coated vascular grafts were implanted to the carotid arteries of experimental dogs as interposed autograft. Implanted grafts were removed after 3 and 6 weeks. As a control, PTFE graft was interposed on carotid artery. These experiments demonstrated that extracelluar matrix produced by fibroblast can afford a base for endothelial cell linings of polyurethane graft. Although thrombosis were developed on autografted en othelial cell coated graft, 33% opening was noticed, and showed less adhesion to adjacent tissue layer. These findings suggest that fiboblast produced extracelluar matrix which can be used for edothelial cell lining vascular graft, and by improving the cultured endothelial cell function, there will be a new modality for reducing thrombosis on small vascular graft.

• Korean Journal of Veterinary Research
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• v.33 no.1
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• pp.145-149
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• 1993

실험대상(實驗對象)으로서 유우(乳牛) 5두(頭)에게 50% 포도당(葡萄糖) $500m{\ell}$를 생리적식염수(生理的食鹽水)에 혼합(混合) 13~15회(回) 반복(反復) 경정맥(jugular vein)을 통해 주입후(注入後) 혈중(血中) insulin수준(水準)과 Langerhans islets의 조직화학(組織化學)을 관찰(觀察)한바, 혈중(血中) insulin의 수준(水準)은 포도당(葡萄糖)의 투여전(投與前)에 비(比)하여 4~11배(倍)나 증가(增加)됨이 관찰(觀察)되었다. 한편, Langerhans islet의 B cell도 포도당(葡萄糖)의 투여회수(投與回數)에 따라 특이(特異)하게 주름이 관찰(觀察)되었는데 이는 B cell에서의 insulin 합성능(合成能)을 높이는데 그 원인(原因)이 있었다고 본다.

• Journal of Chest Surgery
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• v.35 no.8
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• pp.608-610
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• 2002

Lung cancer with pleural seeding has poor prognosis and is generally treated by intravenous anticancer chemotherapy only. We performed intrapleural perfusion hyperthermic-chemotherapy in two lung cancer patients with pleural seeding. Herein, we report our outcome with literature review.

• 대한핵의학회:학술대회논문집
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• 1980.05a
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• pp.6.2-7
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• 1980

간장에 공간점유성병변(암, 농양, 낭종성병변, 혈종 등)이 있을 때는 정적간이미징에서 모두 냉구로 나타난다. 따라서 진단적 특이성이 없다. 그러나 감마카메라를 적절히 잘 이용하면 간암과 간농양을 쉽게 그리고 단시간내에 진단 및 감별할 수 있다. 즉 간암이나 간농양이 의심되는 환자에게 핵제제를 이용한 간동맥기에 열구로 나타나서 정맥기에도 계속 더 강한 열구로 나타내게 되며 간섭취기에는 열구의 음영이 점점 약해져서 종말에는 선명한 냉구로 변해 버린다. 그러나 간농양은 간동맥기, 간정맥기 및 간섭취기 모두에게 계속 냉구로 나타나므로 쉽게 간암과 감별진단이 가능하다. 한편 이 검사는 정적간이미징을 위하여 핵제제를 경정맥투여시에 부담없이 시행하게 되므로 루틴화가 가능하며, 앞으로 간암의 조기진단에 크게 기여하리라 확신한다.