• Journal of Applied Biological Chemistry
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• 제58권2호
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• pp.139-143
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• 2015

본 연구는 다양한 효능을 지닌 초과(Amomum tsao-ko Crevost et Lemaire)의 안전한 이용을 위한 독성평가로 식품의약품안전처 고시 제2014-6호 '의약품등의 독성시험기준'에 맞는 독성시험법에 따라 Balb/c mouse를 이용하여 3주간 반복경구투여를 통해 초과의 안전성을 확인하고자 하였다. 3주간 반복 경구투여 후 체중, 장기중량 측정, 혈액분석 및 혈액생화학 검사를 실시하여 안전성을 확인 한 결과, 초과에 의한 특별한 증상이나 체중, 장기중량의 변화는 관찰되지 않았으며, 복대동맥으로부터 채혈한 혈액을 통한 혈구분석결과에서도 대조군과 초과 추출물 투여군 간의 통계적인 유의성을 관찰 할 수 없었다. 또한 혈청을 이용하여 간기능(GOT, GPT, LDH, ALB, TP-S, T-BIL, D-BIL), 신장기능(BUN, CRE), 지질영양 관련(TG), 전해질 관련(I.P) 지표들의 생화학분석을 수행한 결과, 대조군과 유사하게 모두 정상 범위 내의 결과를 나타내었다. 이러한 결과를 통하여 초과 추출물의 최대무독성용량은 최고 투여량인 2000 mg/kg 이상으로 판단되며, 본 연구결과는 초과의 기능성 식품, 화장품, 의약품 등 다양한 소재로서의 활용에 안전성 관련 기초자료로 이용될 수 있을 것으로 사료된다.

• 한국임상수의학회지
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• 제24권3호
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• pp.308-311
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• 2007

본 연구는 육계에 톨투라주릴을 체중당 10 mg 및 40 mg 용량으로 경구 투여한 후 톨투라주릴의 약물동태학적 분석을 실시하였다. 혈장내의 톨투라주릴의 정량은 액체크로마토그래프/질량분석기를 사용하였으며, 경구 투여후 혈장 농도-시간 자료는 non-compartmental analysis를 이용하여 분석하였다. 톨투라주릴을 10 mg/kg 및 40 mg/kg 용량으로 각각 경구투여 후 혈중최고농도($18.04{\pm}5.50{\mu}g/mL$ 및 $47.15{\pm}9.40{\mu}g/mL$)는 $4.33{\pm}1.51h$ 및 $3.67{\pm}1.51h$에 나타났다. 소실반감기는 $11.40{\pm}4.68h$ 및 $11.64{\pm}4.08h$으로 각각 나타났다. 톨투라주릴의 경구투여 후 혈중최고농도 및 곡선하면적은 용량이 증가함에 따라 증가하였다.

• Environmental Analysis Health and Toxicology
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• 제1권1호
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• pp.81-86
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• 1986

$^{14}$ C로 표지된 $N^{G}$ -mono［$^{14}$ C-methyl］-L-arginine을 흰쥐에 경구투여 하여 7일동안에 66.3%의 방사능이 회수되었다. 회수된 총방사능의 86.2%는 처음 24시간내에 배설되었으며, 그 분포는 산성 물질, 염기성물질, 중성물질 및 회수된 $N^{G}$ -monomethyl-L-arginine에 서 각각 33.3%, 40.2%, 12.5% 및 0.3%이었다. 중성물질 방사능의 약 50%는 N-monomethylurea에 해당하며 이는 투여한 전체방사능의 6%이었다.

• 약학회지
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• 제38권1호
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• pp.46-56
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• 1994

Daily oral administration to Sprague Dawly rats for 4 weeks of DWP-301, at doses of 0, 500, 1000, 2000 mg/kg presented following results; 1) No animals died and there were no significant differences in general signs, body weight, food consumption, urinalysis haematological, biochemical, gross pathological and histopathological examination between control and treated rats. 2) Water consumption, pH-, protein- urobilinogen-, ketone-values in urine were significantly increased in the treated male and female rats. It is supposed that these differences in animals are a consistent feature of repeated overdosage with test suspensions. The results indicate that the non toxic dose of test compounds in rats is over 2000 mg/kg in this test system.

• Biomolecules & Therapeutics
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• 제9권1호
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• pp.46-50
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• 2001

The current study was performed to determine the acute oral toxicity of a crude extract of sanghwang mushroom (Phellinus linteus), in SD rats. 5 rats of each sex were orally treated with a single dose of extract of sanghwang mushroom at doses of 0, 500, 1,000, 2,000 mg/kg, respectively. After the treatment, clinical signs and body weight change, the food and water consumption were observed for 14 days. All animals survived during the study and did not show any clinical signs. Body weight gain showed no significant difference between the control and treated rats. However, body weight gain delayed in high dose group (2,000 mg/kg) on day 1~3 after administration. Another 5 rats of each sex were orally treated with a single dose of extract of sanghwang mushroom at dosages 4,000, 5,000 mg/kg respectively, but all animals survived during the study and did not show any clinical signs. It is suggested that LD$_{50}$ of extract of sanghwang mushroom by oral administration was estimated to be over 5,000 mg/kg in both sexes of rats.s.

• 한국환경보건학회지
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• 제40권4호
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• pp.294-303
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• 2014

Objectives: Excretion and tissue distribution of titanium oxide nanoparticles were evaluated in rats after oral administration. The relation between toxicity and systemic concentration of nanoparaticles was investigated. Methods: Rats were orally treated with titanium oxide nanoparticles (10, 100 mg/kg) for five consecutive days. General toxicity, blood chemistry, and serum biochemical analysis were analyzed. Titanium concentration in liver, kidney, lung, urine and feces were measured and histopathology was performed in these organs. Results: Induction of toxicological parameters was not observed and titanium nanoparticles were excreted via feces. Conclusion: Absorption of titanium oxide nanoparticles via the gastrointestinal tract after oral administration was very poor and systemic concentration of titanium oxide nanoparticles was not elevated. Titanium oxide nanoparticles did not cause toxicities in rats after oral administration.

• Biomolecules & Therapeutics
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• 제6권4호
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• pp.412-416
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• 1998

Acute oral toxicity of Bifidobacterium breve K-110, Bifidobacterium breve K-111, Bifidobacterium infantis K-525 were studied in Sprague-Dawley rats of both sexes. In this study, we examined number of deaths, clinical signs, bod weight and gross findings for 14 day after single oral administration of B. breve K-110,B. breve K-111 or B. infantis K-525 with different levels. They did not show any toxic effect in rats and oral LD$_{50}$ value was over 5 g/kg in rats.s.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2000년도 추계수산관련학회 공동학술대회발표요지집
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• pp.307-308
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• 2000

어류에 축적된 유기화합물은 생식소와 생식세포에 직접적인 작용으로 수정을 저해(Spies et al., 1988)하고 부화율(Hose et al., 1982)을 감소시킬 수 있다. Bishenol A(BPA)는 음식용기와 음료 포장재료로 사용되고 있으며, 캔 용기, 병 뚜껑 그리고 물 공급용 파이프 등에 금속을 코팅하는 물질이다. 이 연구는, 내분비장애물질이 생물의 번식력과 종 연속성에 미치는 영향을 규명하는 연구의 일환으로, 송사리, Oryzias latipes의 수정란을 BPA에 침적처리 하였을 때 수정난의 초기발생에 미치는 영향과 송사리 어미에 BPA를 경구투여 하였을 때 산란량과 부화율에 미치는 영향을 번식생물학적 측면에서 탐색하였다 (중략)

• 대한수의학회지
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• 제36권3호
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• pp.571-575
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• 1996

Sulfamethazine sodium was orally administrated to Sprague Dawley female rats(body weight: 200~250g) with the sonde caude at the dose of 20mg of sulfamethazine sodium per 100g of body weight for 3 days to investigate the depletion rate of the drug from liver, kidney and muscle of rat. The results obtained were summerized as follows; 1. The mean concentrations of sulfamethazine in liver according to the time lapsed after oral administration of the sulfamethazine sodium were decreased from 1.27ppm at day 1 to 0.28ppm at day 4. 2. Sulfamethazine concentrations in kidney according to the time lapsed after oral administration of the sulfamethazine sodium were decreased from 0.77ppm at day 1 to 0. 12ppm at day 4. 3. The mean concentration of sulfamethazine in skeletal muscle according to the time lapsed after oral administration of the sulfamethazine sodium was at or below 0.09ppm within 4 days after withdrawl of medicated solution.

• 한국임상수의학회지
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• 제4권1호
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• pp.423-432
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• 1987

The present studies were undertaken in attempt to investigate the therapeutic effect of ethanol in dogs intoxicated with ethylene glycol Three dogs treated with ethylene glycol and other three dogs with ethylene glycol plus 20% ethanol orally were examined on clinical signs, endoscopic views, histopathological findings, and autopsy findings respectively. The results obtained were summarized as fellows : 1. The clinical sings and their severity of dogs intoxicated with ethylene glycol were time related and progressed from vomiting, depression, thirsty, tachycardia, tachypnea, convulsiot ataxia, melena, uremia and coma, but clinical signs of dogs treated with ethylene glycol and ethanol simultaneously only stowed vomiting and thirsty. 2. In the gastroscopic view, the dogs intoxicated with ethyelne glycol showed edematous, hyperemia, errosive and ulcerative lesions in the fundus and body area but the dogs treated with ethylene glycol and ethanol simultanously showed edematous and hyperemic lesions. 3. Oral treatment of ethanol with ethylene glycol simultaneously have reduced the signs of EG intoxications in dogs.