• Korean Journal of Plant Resources
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• v.31 no.2
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• pp.124-135
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• 2018

The study was performed to explore the molecular changes in the vegetative stage (3-and 5-leaf) of sorghum under waterlogging stress. A total of 74 differentially expressed protein spots were analyzed using LTQ-FT-ICR MS. Among them, 12 proteins were up-regulated and 3 proteins were down-regulated. Mass spectrometry (MS) results showed that about 50% of the proteins involved in various metabolic processes. The level of protein expression of malate dehydrogenase and glyceraldehyde-3-phosphate dehydrogenase related to carbohydrate metabolic process increased in both 3 and 5-leaf stage under waterlogging stress. These proteins are known to function as antistress agents against waterlogging stress. The expression of oxygen-evolving enhancer protein 1 protein related to photosynthesis was slightly increased in the treated group than in the control group, however the expression level was increased in the 5-leaf stage compared to the 3-leaf stage. Probable phospholipid hydroperoxide glutathione peroxidase protein and superoxide dismutase protein related to response to oxidative stress showed the highest expression level in 5-leaf stage treatment. This suggests that the production of reactive oxygen species by the waterlogging stress was the most abundant in the 5-leaf treatment group, and the expression of the antioxidant defense protein was increased.

• Korean Journal of Agricultural Science
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• v.3 no.1
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• pp.67-75
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• 1976

This experiment was studies for grassland establishment on west coastal saline land in Korea from May 1975 to March 1976 and used seeds were introduced from Australia. The results of adaptability were summarized as follow. 1. The seeds germination percentage was not influence in 0.5% salt included, but delayed days in 1% salt included and decreased germination percentage. 2. Any experimental grasses species were not growth on the 0.5% salt included soil and also growth was much damaged on the 0.05% salt included soil. It was suggested that $Mg^+$, $K^+$ ions involved with $Na^+$ ion. 3. The yield of grasses were much decreased on 0.05% salt included soil which compared control group. The yield rate of decrease were 30% in kikuyu, 60% in strawberry clover, 63% in reed canarry and 89% in orchard grass. 4. For winter-hardness test, nearly all the species well overwintered but except kikyu. 5. One of salt tolerance native grass (Agrostis distans crieseb var coreensis, Hackel) was discovered in saline area, and it was growth from middle of May to Middle of September. Also it was known that the native grass was good feed stuff but it was not so long in growth life. 6. It was concluded that more experimental work must be carried out to select of salt tolerance grass for grassland establishment on saline by introduce some more species such like saltgrass, saltbush, shadsclae from foreign land.

• Korean Journal of Poultry Science
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• v.34 no.4
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• pp.259-269
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• 2007

This study was compared the effect of shelf-life and DHA accumulation in chicken meat from broilers fed experimental diets for two weeks($21{\sim}35$ days) of growers. Two hundred-ten male Ross broilers, 1 day of age, were randomly allocated to seven treatment groups. Experimental diets were assigned to each of the seven groups: control diet containing tallow, T1 with 1.00% fish oil, T2 with 2.00% fish oil, T3 with 2.00% fish oil, 200 ppm vitamin E and 200 ppm vitamin C, T4 with 2.00% fish oil and 200 ppm vitamin C, T5 with 2.00% fish oil and 200 ppm vitamin E, and T6 with 3.00% fish oil. The levels of DHA in chicken meat was the highest in T6, and T1 in breast muscle and thigh muscle with skin was higher than that of $T2{\sim}T5,\;T2{\sim}T5$ in raw chicken meat and wing with skin was about two-fold higher than that of T1. The contents of DHA in chicken meat according to storage days were significantly reduced to 42.30%, 49.38% and 48.51% in T1, T2 and T6, respectively, and this decrease was higher than that of T3, T4 and T5 (p<0.05). Particularly, the rate of reduction of DHA was the lowest in the T3 and T5, which were the lowest in TBARS(thiobarbituric acid reactive substances). TBARS increased in the order of T6, T2 and T1, but reduced in the order of T3, T5 and T4 according to storage days, and there was a significant difference among the treatment groups (p<0.05).

• Journal of Veterinary Clinics
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• v.23 no.1
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• pp.72-76
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• 2006

A 1-year-old, female English cocker spaniel (ECS) dog was presented with 3-month history of vomiting and retaking of the vomitus, and chronic weight loss. The client had noticed mild abdominal distension 10 days before. The dog was diagnosed as chronic hepatitis with hepatic cirrhosis based on complete blood count (CBC), serum chemistry profiles, radiography, ascites assessment, bile acid evaluation, and liver biopsy through exploratory laparotomy and necropsy. CBC and serum chemistry profiles revealed mild anemia, slightly elevated hepatic enzymes (ALT and AST), increased creatinine kinase (CK), hyperammonemia, and hypoproteinemia with hypoalbuminemia. Ascites was transudate according to analysis of components. Bile acid assessment (fasting; $174.4{\mu}mol/L$ and postprandial; $198.4{\mu}mol/L$) showed strongly suspected hepatic insufficiency. On radiological findings, ascites was evident. Atrophied liver (especially left side lobes) and distended mesenteric vasculatures were observed by exploratory laparotomy. Histopathological examination of marginal lesion of left lateral lobe of liver by biopsy revealed the necrosis of hepatic cells, dilation of sinusoids, infiltration of neutrophils in sinusoids, and vacuolation of hepatic cytoplasm. The patient had been managed with careful low protein diet and specific supportive therapy (ursodeoxycholic acid, prednisolone, vitamine E, and interferon). Vomiting and ascites disappeared with medical management. The dog was monitored periodically by CBC, serum chemistry and radiographic examination. The dog survived more 18 months with medical therapy. After spontaneous death, necropsy and histopathologic examination were performed.

• Journal of Veterinary Clinics
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• v.29 no.2
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• pp.160-164
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• 2012

The goat industry has been developing for many years in Korea to meet demands for food and medicine. However, many complicated patterns of disease have arisen in goat farms as this industry has developed. In this study, disease occurrence patterns in Korean black goats were surveyed in six professional farming households in Imsil and Soonchang in the Jeonbuk province and in Hamyang and Sachon in the Gyeongnam province to understand and extend the goat disease database. We observed morbidity rates between 2.0% and 9.8% for adult goats and between 2.9% and 68.3% for kids. Kids showed a markedly higher incidence of disease when compared to adults. The rate of disease occurrence was 40.0% for floppy kid syndrome (FKS), 37.7% for diarrhea, 16.0% for respiratory disease, and 1.9% for skin disease. The observed mortality rates were 0.7% ~ 10.0%, and 2.2% ~ 24.9% for adult goats and kids, respectively. In addition, FKS, diarrhea, and respiratory disease were observed in 38.3%, 28.9%, and 10.0%, respectively, of dead goats. In conclusion, the majority of diseases in goats occur during the neonatal period, and FKS is the highest single cause of mortality in Korean black goats. Thus careful attention must be paid to kids to reduce the goat mortality rate.

• Radiation Oncology Journal
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• v.15 no.1
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• pp.57-64
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• 1997

Purpose : Before we report the results of curative radiotherapy in cervix cancer patients, we review the significance and safety of our dose specification methods in the brachytherapy system to have the insight of the potential Predictive value of doses at specified points. Matersials and Methods : We analyze the 리5 cases of cervix cancer patients treated with intracavitary brachytherapy in the lateral simulation film we draw the isodose curve and observe the absorbed dose rate of point A, the reference point of bladder(SBD) and rectum(SRD). In the sagittal view of Pelvic MRI film we demarcate the tumor volume(TV) and determine whether the prescription dose curve of point A covers the tumor volume adequately by drawing the isodose curve as correctly as possible. Also we estimate the maximum Point dose of bladder(MBD) and rectum(MRD) and calculate the inclusion area where the absorbed dose rate is higher than that of point A in the bladder(HBV) and rectum(HRV), respectively. Results : Of forty-five cases, the isodose curve of point A seems to cover tumor volume optimally in only 24(53%). The optimal tumor coverage seems to be associated not with the stage of the disease but with the tumor volume. There is no statistically significant association between SBD/SRD and MBD/MRD, respectively. SRD has statistically marginally significant association with HRV, while TV has statistically significant association with HBV and HRV. Conclusion : Our current treatment calculation methods seem to have the defect in the aspects of the nonoptimal coverage of the bulky tumor and the inappropriate estimation of bladder dose. We therefore need to modify the applicator geometry to optimize the dose distribution at the position of lower tandem source. Also it appears that the position of the bladder in relation to the applicators needs to be defined individually to define 'hot spots'.

• Korean Journal of Plant Resources
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• v.22 no.5
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• pp.365-380
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• 2009

Flora of vascular plants in mountains located at Wonju-si and Hoengseong-gun areas such as Chiak-san(1,288m), Taegi-san(1,261.4m), Obong-san(1,126.2m), Eungbong-san(1,094.9m), Eodab-san(789.4m), Deokgo-san(521.2m), Deokga-san(700.5m) and Seongji-bong(791m) was investigated from April, 2008 to May, 2009, and results from the previous researches in which voucher specimens had been presented from Balgyo-san(998.4m), Oeum-san(930.4m), Baekun-san(1,037.1m) and Chiak-san(1,288m) were included in the list of vascular plants from these areas. This investigations resulted in 804 taxa consisted of 680 species, 1 subspecies, 111 varieties, and 12 forms of 383 genera under 97 families, and totally in 973 taxa (21.14% of all vascular plants in Korea) of 818 species, 1 subspecies, 138 varieties and 16 forms of 418 genera under 105 families on addition of voucher specimens in the previous researches. Forests of the investigated areas were generally mixed of Pinus densiflora and deciduous trees. The areas with comparatively excellent vegetation were valley from Guryong-sa (temple) to Biro-bong (summit) via Seryeom-pokpo (fall) in Mt. Chiak-san, and Keunseong-gol (valley) and eastern slope from Taegibungyo-teo to Naksu-dae (fall) in Mt. Taegi-san. 10 families with abundantly collected species were Compositae, Graminae, Rosaceae, Ranunculaceae, Leguminosae, Cyperaceae, Liliaceae, Saxifragaceae, Umbelliferae and Labiatae in order, and they occuied 49.12% of all collected taxa. Endemic plants found in these areas were 38 taxa including Hanabusaya asiatica, Megaleranthis saniculifolia, and Pyrus ussuriensis var. diamantica, and rare and endangered ones were 24 taxa including Hanabusaya asiatica, Viola websteri, Viola diamantica, and Patrina saniculaefolia. Specially designated plants by the Ministry of Environment were 88 taxa including 12 taxa of 5th degree such as Woodsia intermedia, Hanabusaya asiatica, Equisetum pratense, Iris koreana, Lilium cernum, Trillium tschonoskii, Magnolia kobus(cultivated), Gastrodia elata, Polypodium virginianum, Cimicifuga heracleifolia, Megaleranthis saniculifolia and Viola websteri. 47 taxa of alien plants were found. As to 609 taxa (13.23% of all vascular plants in Korea) of useful plants, 334 taxa for the edible, 269 taxa for the medicinal, 127 taxa for the ornamental, 332 taxa for the forage, 3 taxa for the industrial raw material, 31 taxa for the timber and 13 taxa for the fiber were classified, respectively.

• Korean Journal of Microbiology
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• v.39 no.3
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• pp.166-174
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• 2003

Microcystin produced by cyanobacteria in surface waters, such as eutrophic lake and river, is a kind of serious environmental problems due to its toxicity to human and wild animals. Microcystin is synthesized nonribosomally by the large modular multi-functional enzyme complex known as microcystin synthetase encoded by the mcy gene cluster. Amplification of mcy genes by PCR from cultures and environmental samples is a simple and efficient method to detect the toxigenic Microcystis. In order to evaluate primers designed to detect toxic microcystin-producing strains, 17 cyanobacterial strains and 20 environmental samples were examined by PCR with 7 pairs of primers. Some microcystin-producing cyanobacteria were not detected with FAA-RAA, TOX4F-TOX4R and FP-RP primers. The fragment of unexpected size was amplified with NSZW2-NSZW1 primers in Microcystis strains isolated from the lakes in Korea. TOX1P-TOX1F primers failed in amplification of toxin-producing strains. Only MSF-MSR and TOX2P- TOX2F primers amplified the fragments of mcy genes from 11 strains of microcystin-producing Microcystis. The water samples taken from 20 lakes in Korea were analyzed by PCR using each of the primers. In all the water samples, cyanobacteria capable of producing microcystin were detected by the PCR with TOX2P-TOX2F primers. These results indicate that TOX2P-TOX2F primers are better than the other primers for detection of microcystin-producing Microcystis strains in Korea. The nucleotide sequences of mcy gene in Microcystis aeruginosa NIER10010 suggest genetic diversity of Korean isolates.

• The Journal of Korean Society for Radiation Therapy
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• v.26 no.1
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• pp.69-76
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• 2014

Purpose : To evaluate the changes of the motion of abdominal cavity between interfraction and intrafraction by using abdominal compression for reducing abdominal motion. Materials and Methods : 60 MVCT images were obtained before and after tomotherapy from 10 prostate cancer patients over the whole radiotherapy period. Shift values ( X -lateral Y -longitudinal Z -vertical and Roll ) were measured and from it, the correlation of between interfraction set up change and intrafraction target motion was analyzed when applying abdominal compression. Results : The motion changes of interfraction were X-average $0.65{\pm}2.32mm$, Y-average $1.41{\pm}4.83mm$, Z-average $0.73{\pm}0.52mm$ and Roll-average $0.96{\pm}0.21mm$. The motion changes of intrafraction were X-average $0.15{\pm}0.44mm$, Y-average $0.13{\pm}0.44mm$, Z-average $0.24{\pm}0.64mm$ and Roll-average $0.1{\pm}0.9mm$. The average PTV maximum dose difference was minimum for 10% phase and maximum for 70% phase. The average Spain cord maximum dose difference was minimum for 0% phase and maximum for 50% phase. The average difference of $V_{20}$, $V_{10}$, $V_5$ of Lung show bo certain trend. Conclusion : Abdominal compression can minimize the motion of internal organs and patients. So it is considered to be able to get more ideal dose volume without damage of normal structures from generating margin in small in producing PTV.

• The Journal of Korean Society for Radiation Therapy
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• v.26 no.1
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• pp.59-67
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• 2014

Purpose : This study aims to evaluate 3D dosimetric impact for MIP image and each phase image in stereotactic body radiotherapy (SBRT) for lung cancer using volumetric modulated arc therapy (VMAT). Materials and Methods : For each of 5 patients with non-small-cell pulmonary tumors, a respiration-correlated four-dimensional computed tomography (4DCT) study was performed. We obtain ten 3D CT images corresponding to phases of a breathing cycle. Treatment plans were generated using MIP CT image and each phases 3D CT. We performed the dose verification of the TPS with use of the Ion chamber and COMPASS. The dose distribution that were 3D reconstructed using MIP CT image compared with dose distribution on the corresponding phase of the 4D CT data. Results : Gamma evaluation was performed to evaluate the accuracy of dose delivery for MIP CT data and 4D CT data of 5 patients. The average percentage of points passing the gamma criteria of 2 mm/2% about 99%. The average Homogeneity Index difference between MIP and each 3D data of patient dose was 0.03~0.04. The average difference between PTV maximum dose was 3.30 cGy, The average different Spinal Coad dose was 3.30 cGy, The average of difference with $V_{20}$, $V_{10}$, $V_5$ of Lung was -0.04%~2.32%. The average Homogeneity Index difference between MIP and each phase 3d data of all patient was -0.03~0.03. The average PTV maximum dose difference was minimum for 10% phase and maximum for 70% phase. The average Spain cord maximum dose difference was minimum for 0% phase and maximum for 50% phase. The average difference of $V_{20}$, $V_{10}$, $V_5$ of Lung show bo certain trend. Conclusion : There is no tendency of dose difference between MIP with 3D CT data of each phase. But there are appreciable difference for specific phase. It is need to study about patient group which has similar tumor location and breathing motion. Then we compare with dose distribution for each phase 3D image data or MIP image data. we will determine appropriate image data for treatment plan.