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Primer Evaluation for the Detection of Toxigenic Microcystis by PCR  

이현경 (충북대학교 미생물학과 및 바이오연구소)
김준호 (충북대학교 미생물학과 및 바이오연구소)
유순애 (배재대학교 생물학과)
안태석 (강원대학교 환경학과)
김치경 (충북대학교 미생물학과 및 바이오연구소)
이동훈 (충북대학교 미생물학과 및 바이오연구소)
Publication Information
Korean Journal of Microbiology / v.39, no.3, 2003 , pp. 166-174 More about this Journal
Abstract
Microcystin produced by cyanobacteria in surface waters, such as eutrophic lake and river, is a kind of serious environmental problems due to its toxicity to human and wild animals. Microcystin is synthesized nonribosomally by the large modular multi-functional enzyme complex known as microcystin synthetase encoded by the mcy gene cluster. Amplification of mcy genes by PCR from cultures and environmental samples is a simple and efficient method to detect the toxigenic Microcystis. In order to evaluate primers designed to detect toxic microcystin-producing strains, 17 cyanobacterial strains and 20 environmental samples were examined by PCR with 7 pairs of primers. Some microcystin-producing cyanobacteria were not detected with FAA-RAA, TOX4F-TOX4R and FP-RP primers. The fragment of unexpected size was amplified with NSZW2-NSZW1 primers in Microcystis strains isolated from the lakes in Korea. TOX1P-TOX1F primers failed in amplification of toxin-producing strains. Only MSF-MSR and TOX2P- TOX2F primers amplified the fragments of mcy genes from 11 strains of microcystin-producing Microcystis. The water samples taken from 20 lakes in Korea were analyzed by PCR using each of the primers. In all the water samples, cyanobacteria capable of producing microcystin were detected by the PCR with TOX2P-TOX2F primers. These results indicate that TOX2P-TOX2F primers are better than the other primers for detection of microcystin-producing Microcystis strains in Korea. The nucleotide sequences of mcy gene in Microcystis aeruginosa NIER10010 suggest genetic diversity of Korean isolates.
Keywords
cyanobacteria; mcy gene; microcystin; Microcystis; PCR;
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