• Korean Journal of Plant Tissue Culture
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• v.24 no.6
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• pp.369-374
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• 1997

Immature flower buds of 'Desio' carnation were cultured on MS agar medium supplemented with 1 ㎎/L 2,L-D. Embryogenic calli were formed from 5-10% of the buds less than 20 ㎜ in length, but only non-embryogenic calli were produced from explants of shoot apex leaf, internode, and flowere buds larger than 20 ㎜. The same method was applied to 16 cultivars of cut Sower carnation and embryogenic calli were obtained in 7 cultivars. Several embryogenic callus lines were selected and maintained through subcultures over 120 weeks without loss of embryogenic competence. The embryogenic cultures were also proliferated rapidly in liquid agitation cultures using MS medium supplemented with 1mg/L 2,4-D. Numerous embryos were formed on the periphery of the cell aggregates upon transfer to auxin-free MS agar medium. Plantlets were transplanted in potting soil and grown to bloom in six months.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.3
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• pp.334-339
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• 1995

Removal of apical ear after silking is a method to increase seed production in corn inbreds. Due to the phenomenon of apical dominance, the subapical ear would develope and produce seeds following the removal of the apical ear. This experiment was conducted to investigate the varietal difference in subapical ear development and seed production. Days to silking of subapical ears were delayed by about 4 days compared to normal apical ears. Cob length and filled ear length of subapical ears across seven inbreds tested were reduced by 21% and 36%, respectively. Those of inbred INV302 decreased less and subapical ear of inbred NC246 were barren, which incicated the varietal defferences. Number of kernel rows, kernels per row, and total kernels per ear also decreased. The mean reduction rates for those characters were 28%, 37%, and 47%, respectively. Weights of ears and kernels were reduced and showed different response among inbred lines. Coefficients of variations for cob and filled ear lengths, number of kernel rows and kernels were greater in subapical ears than in apical ears. Seed production of subapical ears was 40 ~ 80% of apical ears, depending on inbred lines when the apical ear is removed immediately after silking.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.32 no.2
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• pp.181-187
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• 1987

The objectives of the study were to reduce lodging during the stormy rain period of soybean growing season in Korea, and to find out the most efficient foliar spraying of pp 333 solution in order to increase tolerance to lodging of soybean plants. The concentration of the pp 333 solution were three, five and eight percent and the time of spraying were 35th day after seeding and at flowering time. The most efficient foliar spraying practice was to spray five percent solution of pp 333 on the 35th day after seeding, resulting in reduced stem length damage of soybeans alone with shortened plant height and stabilized yield of soybean grains.

• Journal of Bio-Environment Control
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• v.15 no.2
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• pp.149-155
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• 2006

This study was carried out to investigate effects of different night temperature treatments during nursery period on flower bud differentiation and growth of pepper cv. Cheongyang. Number of leaves, top fresh weight and top dry weight of pepper seedlings were increased with increasing the night temperature during nursery periods. And also flower bud differentiation and days to flowering were accelerated as increasing the night temperature. Plant height, stem diameter, branch length and intermode length of pepper after transplanting were height at the low night temperature ($28/11^{\circ}C$), but they were retarded at the high night temperature ($28/21^{\circ}C$) treatment. Number of lateral branches was significantly reduced at the high night temperature, but there was no a regular tendency in branching habit of the main stem by temperature treatments. Seedling growth before transplanting was retarded at the low night temperature but gradually recovered after transplanting into the plastic house. However, seedling growth at the high night temperature was shown in contrast to above response of the low night temperature.

• Korean Journal of Plant Resources
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• v.19 no.1
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• pp.83-87
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• 2006

Study on the effective cultivation method for earlier harvest of peony(Paeonia lactiflora Pall.), was carried out in Uiseong Medicinal Plant Experiment Station from 2001 to 2003. Sprouting time and flowering time in house cultivation were earlier than in field cultivation. Planting large crowns of $100{\sim}130g$ increased yield by 34%. The method that is 'Large crown($100{\sim}130g$) + dense planting + vinyl mulching for 2 years' resulted good growth and 54% yield increase. This method made it possible to harvest peony on the second year and reduce productive cost. The yield was decreased more or less in this method but yearly income was increased by 28%.

• Korean Journal of Plant Resources
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• v.19 no.5
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• pp.612-616
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• 2006

In recent years, there has been increasing concerns in gene flow from GM crops to wild or weedy relatives as a potential risk in the commercialization of GM crops. To access the possibility of the environmental impacts by GM rice, small-scale experiments of gene transfer were carried out. Herbicide and drought stress resistant GM rice and non-GM rice Nakdongbyeo, wild rice Oryza nivara, and weedy rice Sharebyeo were used for artificial pollination experiments and bar gene was used as a tractable marker after pollination. The harvested putative hybrid seeds after artificial pollination were germinated and true hybrid plants were selected by basta treatment. The hybrid plants were verified again by PCR amplification of bar and trehalose-6-phosphate phosphatase (TPP) genes and RAPD PCR analysis.

• Journal of Life Science
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• v.21 no.1
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• pp.15-21
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• 2011

Cultivated tomato, Lycopersicum esculentum, is a very important crop. We selected 36 cultivars and studied them for identification and polymorphism by employing random amplified DNA (RAPD) analysis with 80 oligonucleotide primers. Of the 80 primers, 36 primers (45.0%) were polymorphic. Detection of polymorphism in cultivated tomato opens up the possibility of development of its molecular map by judicious selection of genotypes. Molecular markers can also be used for cultivar identification and protection of the plant breeder's intellectual property rights (plant breeders' rights, PBRs). As an example, DNA polymorphism using OPC-13 primer that did not produce the OPC-13-01 band was only found in Junk Pink and Ailsa Craighp cultivars. OPA-12-03 and OPB-15-07 were fragments specific to the TK-70 cultivar and were absent in other cultivars. DNA polymorphism in cultivated tomato in this study was correlated with a type of inflorescence, although some cultivars had exceptions. These approaches will be useful for developing marker-assisted selection tools for genetic enhancement of the tomato plant for desirable traits.

• Proceedings of the Korea Contents Association Conference
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• 2008.05a
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• pp.639-643
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• 2008

Since seasonal changes, blooming of a flower, etc., which take place in accordance with passage of time, transpire over an extended duration of time, one performs the Time-Lapse so that one can watch them quickly by compressing time. Because such a Time-Lapse records a wide range of conditions including the place, angle of view, and time found in a natural state in accordance with a precise interval of time and in the samestate, it is also referred to as Interval recording. The Time-Lapse technique is used widely in various fields such as education, science, documentary, and the media. In terms of acquiring a Time-Lapse image, by making it possible for one to treat an image by deleting and adjusting unnecessary backgrounds excluding the main subject for photography such as the flower unlike existing methods and by making it possible for one to apply extensively the real-life recorded image as a library or image data in 2D or 3D, the present study seeks to propose a technique for the background treatment of Time-Lapse image that allows for everyone to bestow creativity to image production easily and conveniently.

• Korean Journal of Plant Tissue Culture
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• v.25 no.4
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• pp.267-271
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• 1998

Cotyledon explants of lettuce were cocultured with Agrobacterium tumefaciens LBA4404::pBKS-GR1 harboring glutathione reductase(GR) gene in MS medium supplemented with 0.1 mg/L NAA and 1.0 mg/L 2ip for 48 hr. These explants were transferred to MS medium supplemented with 0.1 mg/L NAA and 1.0 mg/L 2ip, 50 mg/L kanamycin, and 500 mg/L carbenicillin. After 4 weeks of subculture, kanamycin-resistant shoots were obtained on selection medium. Leaves of putative transformants survived on selection medium containing 100 mg/L kanamycin. Incoporation of the GR gene into lettuce was confirmed by PCR analysis of genomic DNA. Southern blot analysis showed that ECL-labeled GR gene was hybridized to the expected amplified genomic DNA fragment of about 1.8 kb from transgenic lettuce. The presence of mRNA in transgenic lettuce was confirmed by RT-PCR with total RNA of transgenic lettuce. In progeny test of transformants, R$_1$ seeds were resistant to kanamycin (200mg/L) on MS medium.

• Korean Journal of Plant Resources
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• v.34 no.5
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• pp.451-461
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• 2021

In Jeju Island, there are two different types of Opuntia cactus in cladodae, fruit, flower, flowering period and spine. There is no comparative study of these two taxa yet, so it is often seen as the same species. The purpose of this study was to investigate the phylogenetic relationship between the Taxa of the Opuntia monacantha Haw. and Opuntia stricta Haw. in Jeju Island. The results indicate that the two taxa were completely different from each other on the analysis of nrITS, matK and atpB-rbcL gene as well as the morphological comparison. Also, O. monacantha among the two taxa is included in the Elatae series, so South America is the origin, and O. stricta is included in the Scheerianae series, which confirms that North America is the origin. The name of the O. monacantha in Jeju Island was named 'Jeju-Baick-nyun-cho' considering geographical location conditions and symbolic characteristics.