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The Investigation of Gene Flows in Artificial Pollination between GM Rice and its Wild Relatives by RAPD Analysis  

Kim, Yoon-Sik (Plant Cell Biotechnology Lab., Korea Research Institute of Bioscience and Biotechnology)
Kim, Hyun-Soon (Plant Cell Biotechnology Lab., Korea Research Institute of Bioscience and Biotechnology)
Joung, Hyouk (Plant Cell Biotechnology Lab., Korea Research Institute of Bioscience and Biotechnology)
Jeon, Jae-Heung (Plant Cell Biotechnology Lab., Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Korean Journal of Plant Resources / v.19, no.5, 2006 , pp. 612-616 More about this Journal
Abstract
In recent years, there has been increasing concerns in gene flow from GM crops to wild or weedy relatives as a potential risk in the commercialization of GM crops. To access the possibility of the environmental impacts by GM rice, small-scale experiments of gene transfer were carried out. Herbicide and drought stress resistant GM rice and non-GM rice Nakdongbyeo, wild rice Oryza nivara, and weedy rice Sharebyeo were used for artificial pollination experiments and bar gene was used as a tractable marker after pollination. The harvested putative hybrid seeds after artificial pollination were germinated and true hybrid plants were selected by basta treatment. The hybrid plants were verified again by PCR amplification of bar and trehalose-6-phosphate phosphatase (TPP) genes and RAPD PCR analysis.
Keywords
GM rice; Gene flow; RAPD;
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