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The Investigation of Gene Flows in Artificial Pollination between GM Rice and its Wild Relatives by RAPD Analysis  

Kim, Yoon-Sik (Plant Cell Biotechnology Lab., Korea Research Institute of Bioscience and Biotechnology)
Kim, Hyun-Soon (Plant Cell Biotechnology Lab., Korea Research Institute of Bioscience and Biotechnology)
Joung, Hyouk (Plant Cell Biotechnology Lab., Korea Research Institute of Bioscience and Biotechnology)
Jeon, Jae-Heung (Plant Cell Biotechnology Lab., Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Korean Journal of Plant Resources / v.19, no.5, 2006 , pp. 612-616 More about this Journal
Abstract
In recent years, there has been increasing concerns in gene flow from GM crops to wild or weedy relatives as a potential risk in the commercialization of GM crops. To access the possibility of the environmental impacts by GM rice, small-scale experiments of gene transfer were carried out. Herbicide and drought stress resistant GM rice and non-GM rice Nakdongbyeo, wild rice Oryza nivara, and weedy rice Sharebyeo were used for artificial pollination experiments and bar gene was used as a tractable marker after pollination. The harvested putative hybrid seeds after artificial pollination were germinated and true hybrid plants were selected by basta treatment. The hybrid plants were verified again by PCR amplification of bar and trehalose-6-phosphate phosphatase (TPP) genes and RAPD PCR analysis.
Keywords
GM rice; Gene flow; RAPD;
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  • Reference
1 Daniell H. 2002. Molecular strategies for gene containment in transgenic crops. Nature Biotech. 20: 581-586   DOI
2 Ferber, D. 1999. Risks and benefit: GMcrops in the cross hairs. Science. 286: 1622-1666
3 Nielsen, K. M., A. M. Bones, K. Smalla and van J. D. Elass. 1998. Horizontal gene transfer from transgenic plants to terrestrial bacteria- a rare events? FEMS Microbiol. Rew 22: 79-103   DOI
4 Seo, H. S., Y. J. Koo, J. Y. Lim, J. T. Song, S. H. Kim, J. K. Kim, J. S. Lee and Y. D. Choi. 2000. Characterization of a bifunctional enzyme fusion of trehalose-6-phosphate synthetase and trehalose-6-phosphate phosphatase of Eschericha coli. Appl. Environ. Microbiol. 66: 2484-2490
5 Spok, A., H. Gaugitsch, S. Laffer, G. Pauli, H. Saito, H. Sampson, E. Sibanda, W. Thomas, M. van Hage and R. Valenta. 2005. Suggestions for the assessment of the allergenic potential of genetically modified organisms. Int. Arch. Allergy Immunol. 137(2): 167-80   DOI   ScienceOn
6 Doyle, J. J. and J. L. Doyle. 1990. Isolation of plant DNA from fresh tissue. Focus 12: 13-15
7 Dale, P. J., B. Clarke and E. M.G. Fontes. 2002. Potential for the environmental impact of transgenic crops. Nature Biotech. 20: 567-574   DOI
8 Jang, I. C., S. J. Oh, J. S. Seo, W. B. Choi, S. I. Song, C. H. Kim, Y. S. Kim, H. S. Seo, Y. D. Choi, B. H. Nahm and J. K. Kim. 2003. Expression of a bifunctional fusion of the Escherichia coli genes for trehalose-6 phosphate synthase and trehalose- 6 phosphate phsphatase in transgenic rice plants increases trehalose accumulation and abiotic stress tolerance without stunting growth. Plant Physiol. 131: 516-524   DOI   ScienceOn
9 Nosey, J. E., L. S. Rayor and M. E. Carter. 1999. Transgenic pollen harms monarch larvae. Nature 399: 214-214
10 Monastra, G. and L. Rossi. 2003. Transgenic foods as a tool for malnutrition elimination and their impact on agricultural systems. Rivista di Biologia. Biology Forum pp. 363-384
11 Kling, J. 1996. Agricultural Ecology: Could Transgenic Supercrops One Day Breed Superweeds? Science 274: 180–181   DOI
12 D'Agnolo, G. 2005. GMO: Human health risk assessment. Vet. Res. Commun. 2: 7-11
13 Lorenz, M. G. and J. Sikorski. 2000. The potential for intraspecific horizontal gene exchange by natural genetic transformation: sexual isolation among genomovars of Pseudomonas stutzeri. Microbiol. 146: 3081-3090   DOI
14 Conner, A. J., T. R. Glare and J. P. Nap. 2003. The release of genetically modified crops into the environment. The Plant Jour. 33: 19-46   DOI   ScienceOn
15 Snow, A. A. 2002. Transgenic crops – Why gene flow matters. Nature Biotech 20: 542-542
16 Roger, M. A., L. Lamond, C. Preston, S. B. Powles and R. T. Roush. 2002. Pollen-mediated movement of herbicide resistance between commercial calnola fields. Science 296:2386-2388   DOI   ScienceOn