This study was carried out to test the effects of temperatures between 2$0^{\circ}C$ and 3$0^{\circ}C$ on the reproduction and development of the rice water weevil, Lissorhoptrus oryzophilus. Preoviposition periods were much longer (17.2 to 51.0 days) in the overwintering adult females collected in March than those collected in May, regardless of temperature. Oviposition periods, however, were longer (16.9 to 22.0 days) in the adult females collected in May than those collected in March at the same temperatures. The longer oviposition period observed in the females collected in May were directly associated with higher fecundity. Egg periods were shortened from as temperature increased, but the hatching rate was highest (100%) at 27$^{\circ}C$. The developmental periods from egg to adult were shortened as temperature increased : from 77.9 days at 2$0^{\circ}C$ to 38.3 days at 3$0^{\circ}C$. The developmental zero point temperature (T) and the total effect temperature (K) for egg were 16.3$^{\circ}C$ and 62.1 dgree days, respectively. The T and K from egg to adult emergence were 13.9$^{\circ}C$ and 577.6 dgree days, respectively. The adult females of the first generation did not oviposit at 2$0^{\circ}C$, but did at $25^{\circ}C$ and 3$0^{\circ}C$. The intrinsic rate of natural increase (r$_{m}$) increased as temperature augmented. Net reproductive rate (Ro) per generation was highest (75.3) at $25^{\circ}C$.>.
In present study, the effect of various factors including the solvent concentration, extraction time, extraction temperature and the ratio of sample vs extraction solvent(w/v) upon the extractability of the NaOH soluble proteins of marine algae were investigated. Seven species of sun-dried algae, the major ones in consumption as food, namely Porphyra suborbiculata, Undaria pinnatifida(natural and cultivated), Sargassum fulvellum, Sargassum kjellmanianum, Ulva pertusa, Enteromorpha linza and Codium coarctatum were used for the extraction of the NaOH soluble protein. The frozen and masceratd samples were prepared by the same mettled described in previous paper(Lee, 1977). In case of the TCA insoluble protein, all samples reached maxima at 0.025M NaOH solution while the 0.05M for extractable total nitrogen. Variation of the ratio of sample vs solvent gave slight effect upon the extractability, 100 ml solvent added to 1 g dried sample was effective. The effect of extraction time on the extractability differed from species. The extractabilty of Enteromorpha linza, Ulva pertusa and Codium coarctatum reached maxima within 1 hour extraction and 2 hours for the cultivated Undaria pinnatifida while 3 flours for the natural Undaria pinnatifida, Sargassum fulvellum, Sargassum kjellmanianum and Porphyra suborbiculata. The most effective extraction temperature was $60^{\circ}C$ for all samples.
This study was conducted to understand the dynamics of naturally regenerated seedlings and saplings in 28years after selective cutting at natural Korean pine-broadleaved forests in Mt. Changbai. The number of naturally regenerated tree species was 16 species in 28 years after selective cutting. The appearance rate of naturally regenerated tree species was 56 percentage and the early-successional tree species of Acer ukurunduense, Acer mono, Acer pseudosieboldianum, Prunus padus var. pubescens, and Maackia amurensis were found in the first 10 years after selective cutting. The seedlings and saplings of shade-tolerant tree species of Pinus koraiensis, Acer mandshuricum, Ulmus laciniata were appeared and the appearance rate was higher than 75% after 15 years while it showed little changes from 19 years after selective cutting. The distribution density of seedlings and saplings of regenerated tree species in selective cutting area was 5,500~6,100 trees per hectare in the first 10 years, and it increased gradually along with the restoration of disturbed area and it could reach 7,500 trees per hectare after 15 years.
To investigate the effects of elevated $CO_2$ on the denitrifying bacterial community structure in a wetland soil, dynamics of bacterial community structure was explored in an artificial wetland ecosystem with one of three plant species (T. latifolia, S. lacustris, and 1. effusus) under two levels of $CO_2$(370 ppm or 740 ppm) after 110day incubation. For the analysis of bacterial community structure, functional genes such as nitrite reductase genes (nirS) were PCR-amplified followed by cloning of PCR products and screening by restriction fragment length polymorphism (RFLP). nirS gene fragments were amplified in all analyzed soil samples. Species richness estimated by the number of distinct phylotypes were 83 and 95 in the ambient $CO_2$ treatment and the elevated treatment, respectively. Two phylotypes (type 1 and type 2) were dominant in both of the treatments. Elevated $CO_2$ treatment increased species richness of denitrifying as well as changed a large proportion of denitrifier phylotypes compared to those of the ambient treatment. Overall, the data in this study suggested that the denitrifying communities in the wetland soil are diverse and that the richness of denitrifying bacterial community might be affected by elevated $CO_2$ treatment.
The effects of precooling treatments on the storability of chicon were investigated during modified atmosphere (MA) storage. The forced air cooling showed faster precooling rate that reduced the internal temperature of chicon to $2{\pm}1^{\circ}C$, and the precooling time of forced air cooling was 1/6 of room cooling. The half cooling time was 3 hr 21 min in room cooling and 1 hr 17 min in forced air cooling. Weight loss was less than 0.5% in all treatments both $5^{\circ}C$ and $10^{\circ}C$ MA storage and maintained higher in forced air cooling treatment. The concentration of carbon dioxide, oxygen, and ethylene of $50{\mu}m$ ceramic film packages were observed higher at $10^{\circ}C$ than $5^{\circ}C$. The precooling effect on respiration reduction was not shown at $5^{\circ}C$, but appeared that the gas concentration of precooling treatments showed less carbon dioxide and higher oxygen than non precooling treatment by 9 days after $10^{\circ}C$ storage. Ethylene concentration of precooling treatments showed lower than non precooling treatment until 3 days both $5^{\circ}C$ and $10^{\circ}C$ MA storage. Precooling showed the effect on maintaining visual quality of chicon both $5^{\circ}C$ and $10^{\circ}C$ MA storage. However, the forced air cooling that showed faster precooling rate did not appeared more precooling effect on the visual quality than room cooling because the fast air flow (6.0 m/sec) of forced air cooling hit directly on chicon outer leaves and might cause physical damage to chicon. Although the forced air cooling showed the effect on maintaining quality of chicon, but additional studies should be needed that investigated proper air flow rate and cooling box structure can prevent physical damage by air flow.
Global climatic changes are expected to influence various biogeochemical processes in wetland ecosystems. In particular, coastal mud flat is anticipated to be affected directly by temperature increase as well as indirectly by a sea level rise and changes in precipitation. This study aimed to determine changes in methane production under different temperature and salinity by employing a laboratory-scale manipulation experiment. Soil samples were collected from a mud flat in Dong-Gum Kang-Hwa island in winter and two types of experiments were conducted. In the first experiment soil samples at 0-5 cm, 5-10 cm depth were incubated under same salinity with pore water and diluted salinity to 50 % of natural condition for 20 days and methane production was measured every other days. In the second experiment, soil samples at 5-10 cm depth were incubated in different temperature, $5^{\circ}C$ and $15^{\circ}C$, under same salinity conditions with first experiment for 31 days and methane production was measured. Results of the first experiment revealed that higher amount of methane was produced at 5-10 cm depth, and salinity effect was predominant at the end of the experiment. The second experiment showed that methane production was higher in $15^{\circ}C$ than $5^{\circ}C$. In addition, methane production was higher when sea water diluted to 50 % compared to control. Global climatic changes are expected to influence various biogeochemical processes in wetland ecosystems. In particular, coastal mud flat is anticipated to be affected directly by temperature increase as well as indirectly by a sea level rise and changes in precipitation. This study aimed to determine changes in methane production under different temperature and salinity by employing a laboratory-scale manipulation experiment. Soil samples were collected from a mud flat in Dong-Gum Kang-Hwa island in winter and two types of experiments were conducted. In the first experiment soil samples at 0-5 cm, 5-10 cm depth were incubated under same salinity with pore water and diluted salinity to 50 % of natural condition for 20 days and methane production was measured every other days. In the second experiment, soil samples at 5-10 cm depth were incubated in different temperature, $5^{\circ}C$ and $15^{\circ}C$, under same salinity conditions with first experiment for 31 days and methane production was measured. Results of the first experiment revealed that higher amount of methane was produced at 5-10 cm depth, and salinity effect was predominant at the end of the experiment. The second experiment showed that methane production was higher in $15^{\circ}C$ than $5^{\circ}C$. In addition, methane production was higher when sea water diluted to 50 % compared to control. These results suggest that methane production is highly influenced by changes in temperature and salinity in coastal mud flat. And that global climatic change may induce biological feedback by affecting production of another greenhouse gas, namely methane from coastal mud flat.
Journal of the Korea Organic Resources Recycling Association
/
v.6
no.1
/
pp.53-66
/
1998
This study was carried out to investigate the typical composition of food waste from municipal solid wastes (MSW) and unit food waste generation rates from different sources in the model cities and to study the food waste management system from unit household to composting facilities. The annual average food waste composition of MSW was determined as 49% in Taejon and 52% in Chungnam Province, respectively. No big difference in food waste composition was found among the different sources. Since the paper waste generally occupied the half of food waste, over 75% of MSW was found to be compostable or biodegradable. Per capita food waste generation rate, 200~250g/capita day was determined by the direct measurement from 32 households, while 380g/capita day for Chungnam Province and 400 g/capita day for Taejon were estimated by the load count analysis In the sanitary landfills. This difference means people contribute generating food waste at outside house approximately twice as much as that at inside house. Per capita food waste generation rates from several sources were determined as follows; 166~215g/capita day at municipalities, 400g/visitor day at a first class hotel, 170g/student day at a university restaurant. Food waste generation from restaurant was strongly dependant upon it's level or quality; 670g/capita day at the high level restaurant, 190g/capita day at the middle class and 60 g/capita day at the lower class restaurant. The food waste reduction rate in a In situ fermentor showed 30~40g/kg day.
Journal of the Korean Institute of Traditional Landscape Architecture
/
v.32
no.2
/
pp.167-179
/
2014
This study fundamentally is to develop standards and foundations for the establishment of traditional Korean gardens and aims to identify the mechanism and subsequent effect of fair gardens in American and Europe on the propagation and diffusion of the traditional Japanese garden. Fair gardens which were constructed between 1867 and 1939 were investigated to understand them and the ripple effect that they had on cultural dissemination. The results were as follows: Culturally, the Meiji government adopted Wagener's advice on the theme of display- including culture and handicraft-and the gardens with traditional buildings were perceived as one unit and then used as promotional tools as part of a national strategy. As a result, the stroll style garden in the Edo period and tea garden were recognized as the representative Japanese garden in America and Europe. Politically, the Japanese garden in the American context was adopted as examples of 'exotic beauty' and 'cultural heritage' which therefore allowed the Japanese government to achieve it's goal of encouraging friendly relations and the lessening of hostility towards them. Throughout the traditional Japanese garden, Japan with it's rich history presented an ideal - uniquely distinctive from the West. Using 'tradition' and 'nature' as keywords, the Japanese government set it's global image as 'perpetual tranquility'. Socioeconomically, the Japanese garden which was maintained after the fair, played a consistent role as a model of the Japanese culture. Many professionals from Japan who prepared the Japanese villages and gardens for the world fairs in America and Europe, remained in these countries following construction and it were these opportunities that allowed the Japanese garden to be integrated into local Western society.
A line of study reported that electroacupuncture(EA) modulate natural killer cell(NK Cell) activities. One report suggested that EA enhanced splenic interferon-gamma($IFN-{\gamma}$), interleukin-2(IL-2), and NK cell activity in Sprague-Dawley rats. Another study suggested that $IFN-{\gamma}$ mediates the up-regulation of NK cell activity, and endogenous ${\beta}$-endorphin secretion also play a role in the up-regulation of NK cell activity induced by EA stimulation. In order to better understand the molecular regulation underlying the activation of NK cell induced by EA, we have utilized cDNA microarray to elucidate how EA alters program of gene expression of spleen in rats. First, we divided three groups, group I was EA group treated with EA in restriction holder, group II was sham group with only holder stress, and last group III was control group with no treatment. We measured NK cell activity after EA stimulation three times for 2 days using $^{51}Cr$ release assay. Second, Biotin-labeled cDNA probes synthesized from EA group and sham group, were competitively hybridized to the microarray that contained variable genes. Such high-throughput screening has identified a number of EA-responsive gene candidates. Of these, we found that EA induced a subset of genes of genes that functionally could modulatory effects on NK cell activity. Genes(vascular cell adhesion molecule-1, protein-tyrosine kinase, CD94 mRNA) related to boost NK cell activity, were increased by EA And, genes(protein-tyrosine-phospatase mRNA, protein-tyrosine phosphatase(SHP-1) mRNA) related to inhibit NK cell activity, were decreased by EA. These EA-responsive genes may provide key insights from which to understand mechanisms of activation of NK cell induced by EA.
Effects of elevated carbon dioxide ($CO_2$) on soil microbial processes were studied in a northern peatland. Intact peat cores with surface vegetation were collected from a northern Welsh fen, and incubated either under elevated carbon dioxide (700 ppm) or ambient carbon dioxide (350 ppm) conditions for 4 months. Higher algal biomass was found under the elevated $CO_2$ condition, suggesting $CO_2$ fertilization effect on primary production, At the end of the incubation, trace gas production and consumption were analyzed using chemical inhibitors. For methane ($CH_4$ ), methyl fluoride ($CH_3F$) was applied to determine methane oxidation rates, while acetylene ($C_2H_2$) blocking method were applied to determine nitrification and denitrification rates. First, we have adopted those methods to optimize the reaction conditions for the wetland samples. Secondly, the methods were applied to the samples incubated under two levels of $CO_2$. The results exhibited that elevated carbon dioxide increased both methane production (210 vs. $100\;ng\;CH_4 g^{-1}\;hr^{-1}$) and oxidation (128 vs. $15\;ng\;CH_4 g^{-1}\;hr^{-1}$), resulting in no net increase in methane flux. For nitrous oxide ($N_2O$) , elevated carbon dioxide enhanced nitrous oxide emission probably from activation of nitrification process rather than denitrification rates. All of these changes seemed to be substantially influenced by higher oxygen diffusion from enhanced algal productivity under elevated $CO_2$.
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