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Influence of Elevated $CO_2$ on Denitrifying Bacterial Community in a Wetland Soil  

Lee Seung-Hoon (이화여자대학교 환경학과)
Kim Seonyoung (이화여자대학교 환경학과)
Kang Hojeong (이화여자대학교 환경학과)
Publication Information
Korean Journal of Microbiology / v.40, no.3, 2004 , pp. 244-247 More about this Journal
Abstract
To investigate the effects of elevated $CO_2$ on the denitrifying bacterial community structure in a wetland soil, dynamics of bacterial community structure was explored in an artificial wetland ecosystem with one of three plant species (T. latifolia, S. lacustris, and 1. effusus) under two levels of $CO_2$(370 ppm or 740 ppm) after 110day incubation. For the analysis of bacterial community structure, functional genes such as nitrite reductase genes (nirS) were PCR-amplified followed by cloning of PCR products and screening by restriction fragment length polymorphism (RFLP). nirS gene fragments were amplified in all analyzed soil samples. Species richness estimated by the number of distinct phylotypes were 83 and 95 in the ambient $CO_2$ treatment and the elevated treatment, respectively. Two phylotypes (type 1 and type 2) were dominant in both of the treatments. Elevated $CO_2$ treatment increased species richness of denitrifying as well as changed a large proportion of denitrifier phylotypes compared to those of the ambient treatment. Overall, the data in this study suggested that the denitrifying communities in the wetland soil are diverse and that the richness of denitrifying bacterial community might be affected by elevated $CO_2$ treatment.
Keywords
$CO_2$; denitrifying bacterial community; PCR-RFLP; wetland;
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1 Megonigal, J.P., and W.H. Schlesinger. 1997. Enhanced CH$_4$ emissions from a wetland soil exposed to elevated CO$_4$. Biogeochemistry 37: 77-88
2 Smit, E., P. Leeflang, S. Gommans, J. van den Broek, van M.S., and K. Wernars. 2001. Diversity and seasonal fluctuations of the dominant members of the bacterial soil community in a wheat field as determined by cultivation and molecular methods. Appl. Environ. Microbiol. 67, 2284-2291
3 Klamer, M., M.S. Roberts, L.H. Levine, B.G. Drake, and J.L. Garland. 2002. Influnece of elevated CO$_2$ on the fungal community in a coastal scrub oak forest soil investigated with terminal-restriction fragment length polymorphism analyis. Appl. Environ. Microbiol. 68, 4370-4376
4 Bedzyk, L., and R.W. Ye. 1999. The periplasmic nitrate reductase in Pseudomonas sp. strain G-179 catalyzes the first step of denitrification. J. Bacteriol. 175, 1165-1172
5 Berks, B.C., S.J. Ferguson, J.W.B. Moir, and D.J. Ricahrdson. 1995. Enzymes and associated electron transport systems that catalyse the respiratory reduction of nitrogen oxides and oxyanions. Biochim. Biophys. Acta. 1232, 97-173
6 Kang, H., C. Freeman, and T. Ashenden. 2001. Effects of elevated CO$_2$ on fen peat biogeochemistry. Sci. Total Environ. 279, 45-50
7 Liu, X., S.M. Tiquia, G. Holguin, L. Wu, S.C. Nold, A.H. Devol, K. Luo, A.V. Palumbo, J.M. Tiedje, and J. Zhou. 2003. Molecular diversity of denitrifying genes in continental margin sediments within the oxygen-deficient zone off the Pacific coast of Mexico. Appl. Environ. Microbiol. 69, 3549-3560
8 Mitsch, W.J., and Gosselink, J.G. 1993. Wetlands. John Wiley and Sons, New York
9 Odum, E.P. 1983. Basic ecology. Saunders College Publishing, Philadelphia
10 Coyne, M.S., A. Arunakumari, B.A. Averill, and J.M. Tiedje. 1989. Immunological identification and distribution of dissimilatory heme cd1 and non-heme copper nitrite reductases in denitrifying bacteria. Appl. Environ. Microbiol. 55, 2924-2931
11 Gregory, L.G, A. Karakas-Sen, D.J. Richardson, and S. Spiro. 2000. Detection of genes for membrane-bound nitrate reductase in nitrate-respiring bacteria and in community DNA. FEMS Microbiol. Lett. 183, 275-279
12 Chao, A. 1987. Estimating the population size for capture-recapture data with unequal catchability. Biometrics 30, 101-110