• Title/Summary/Keyword: 강성추정

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Analysis and Uncertainty Estimation of Zearalenone in Cereal-Based Products by LC-MS/MS (LC-MS/MS를 이용한 곡류가공품의 제랄레논 분석과 측정불확도 추정)

  • Choi, Eun Jung;Kang, Sung Tae;Jung, So Young;Shin, Jae Min;Jang, Min Su;Lee, Sang Me;Kim, Jung Hun;Chae, Young Zoo
    • Korean Journal of Food Science and Technology
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    • v.44 no.6
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    • pp.658-665
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    • 2012
  • A survey of zearalenone contamination was conducted on cereal-based products by using an immunoaffinity column with LC-MS/MS. The calibration curve showed good lineality, with correlation coefficients ($R^2$) of 0.999 in the concentration range from 1 to 250 ng/mL. The limits of detection and quantification were approximately $0.3{\mu}g/kg$ and $1.0{\mu}g/kg$, respectively. The recoveries in the barley tea, Misutgaru and snack ranged from 73.6-107.8%. Zearalenone was detected in 10 samples (11.2% incidence). The highest zearalenone contamination level was $29.7{\mu}g/kg$ in the Misutgaru. This survey was conducted with uncertainty of measurement. The expanded uncertainty for zearalenone was estimated to be $44.9{\pm}5.0{\mu}g/kg$ (k=2, 95% confidence level) and $128.7{\pm}7.9{\mu}g/kg$ (k=2, 95% confidence level) for barley tea, $30.7{\pm}5.8{\mu}g/kg$ (k=2, 95% confidence level) and $173.7{\pm}14.9{\mu}g/kg$ (k=2.26, 95% confidence level) for Misutgaru, and $37.2{\pm}7.4{\mu}g/kg$ (k=2.31, 95% confidence level) and $151.0{\pm}10.4{\mu}g/kg$ (k=2, 95% confidence level) snack at the level of $41.7{\mu}g/kg$ and $166.7{\mu}g/kg$, respectively.

Antibacterial Activity of Platycarya strobilacea Extract and Stability of the Extract-containing Cream (굴피나무 추출물의 항균 활성 및 추출물을 함유한 크림의 안정성 분석)

  • Yang, Hee-Jung;Kim, Eun-Hee;Kang, Sung-Tae;Park, Soo-Nam
    • Microbiology and Biotechnology Letters
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    • v.37 no.2
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    • pp.170-175
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    • 2009
  • The extract of Platycarya strobilacea is known to possess a wide range of pharmacological activities including anti-inflammatory, anti-fungal, and anti-cancer properties. We have reported that the ethyl acetate fraction of Platycarya strobilacea (PS-ET fraction) has high potential as an antioxidant agent (J. Soc. Cosmet. Scientists Korea 34(4) 275, 2008). In this study, antibacterial activity of the fraction and stability of the cream containing 0.2% PS-ET fraction were investigated for the application to cosmetics. Antibacterial activity of PS-ET fraction against various skin pathogenic bacteria (Propionibacterium acnes, Staphylococcus aureus, and Pityrosporum ovale) was measured by minimum inhibitory concentration (MIC). MIC values of PS-ET fraction on P. acnes, S. aureus, and P. ovale were 0.13%, 0.06% and 0.25%, respectively. The results showed that the antibacterial activity of the fraction was the highest in the S. aureus. For the stability evaluation, pH and viscosity of the cream containing 0.2% PS-ET fraction were measured. The results showed that pH changes of the cream containing PS-ET fraction was lower than the control cream without PS-ET fraction. And the PS-ET fraction could repress the decrease of viscosity of the cream against sunlight treatment. These results suggest that the fraction of Platycarya strobilacea has high potential as bactericide against the skin pathogenic bacteria and could be added to improve the stability of cosmetic products.

Conceptual Design of the Three Unit Fixed Partial Denture with Glass Fiber Reinforced Hybrid Composites (Glass fiber 강화 복합레진을 사용한 3본 고정성 국소의치의 개념 설계 연구)

  • Na, Kyoung-Hee;Lee, Kyu-Bok;Jo, Kwang-Hun
    • Journal of Dental Rehabilitation and Applied Science
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    • v.18 no.3
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    • pp.145-155
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    • 2002
  • The results of the present feasibility study are summarized as follows, 1. The three unit bridge of knitted material and UD fibre reinforcement has both the rigidity and the strength against a vertical occlusal load of 75N. 2. Stress concentration at the junctional area between the bridge and the abutments, i.e. between the pontic and the knitted caps was observed. In the case of the bridge with reinforcement straps, it was partly shown that the concentration problem could be improved by simply increasing the fillet size at the area. Further refining in the surface of the junctional area will be needed to ensure a further improvement in the stress distribution. This will require some trade off in the level of the stress and the available space. A parametric study will help to decide the appropriate size of the fillet. 3. Design refinement is a must to improve the stress distribution and realize the most favourable shape in terms of fabrication. The current straight bar with a constant cross section area can be redesigned to a tapered shape. The curve from the dental arch should also be placed on the pontic design. In accordance with design refinement, the resistance of the bridge frame to other load cases should be evaluated. 4. Although not included in the present feasibility study, it is estimated that bridges of the anterior teeth can be made strong enough with the knitted material without further reinforcement using unidirectional materials. In this regard, a feasibility study on design concepts and stress analysis for 3, 4, 5 unit bridge is suggested. 5. Two types of bridge were analysed in terms of fatigue. The safe life design concept, i.e. fatigue design concept, looks reasonable for the bridge where if cracks should form and propagate there is virtually nothing a dentist to do. The bridge must be designed so that no crack will be initiated during the life span. In the case of crowns, however, if constructed with composite resin with knitted materials, it might be possible to repair them, which in general is impossible for crowns of PFM or of metal. Therefore for composite resin crowns, a damage tolerance design concept can be applied and reasonably higher operational stresses can be allowed. In this case, of course, a periodic inspection program should be established in parallel. 6. Parts of future works in terms of structural viewpoint which need to be addressed are summarized as the following: 1) To develop processing technology to accommodate design concepts; 2) More realistic modelling of the bridge and analysis-geometry and loading condition. Thickness variation in the knitted material, taper in the pontic, design for anterior tooth bridge, the effect of combined loads, etc, will need to be included; 3) To develop appropriate design concepts and design goals for the fibre composite FPD aiming at taking the best advantage of knitted materials, including the damage tolerance design concept; 4) To develop testing method and perform test such as static ultimate load test, fatigue test, repair test, etc, as necessary.

Micrographic Comparison of Proglottids and Ova in Some Tapeworms(Family: Diphyllobothridae) from Man (인체기생 열두조충류의 형태비교 및 진단적 소견)

  • 류장근;양용상;강성구;백승한;임신영
    • Biomedical Science Letters
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    • v.4 no.1
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    • pp.65-72
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    • 1998
  • Recently there have been frequent reports on human infection caused by the Diphyllobothridae in Korea. The adequate opportunities for Koreans to eat raw fish, the primary infection medium of cestodes and the human infection through drinking water by cyclops, the first intermediate host are believed to be main reasons for the infection. The first task of this study was to classify and diagnose the species by differentiating morphological characteristic between scolex and proglottids of cestodes. However, the initially available diagnosis was done with the patient's symptoms and the eggs obtained from his stool. It is important to differentiate the species by the eggs of Diphyllobothrium latum especially in that it can help get advance information for a more reliable analysis in the near future. The morphological and diagnostic results from proglottids and eggs of Diphyllobothrium latum, Diphyllobothrium latum parvum and Spirometra erinacei are as follows; In each kind of cestodes from the patient's stool, the shape and size of 50 eggs were measured. Eggs of Diphyllobothrium latum had an operculum and were ovoidal or ellipsoid to elliptical in shape. Eggs of Diphyllobothrium latum parvum were more ovoidal in shape and smaller in size than Diphyllobothrium latum. And eggs of Spirometra erinacei were asymmetrical in width and long and slender in shape. The average lengths and widths of Diphyllobothrium latum, Diphyllobothrium latum parvum and Spirometra erinacei were 61.4$\times$41.7 $\mu\textrm{m}$, 55.9$\times$41.4 $\mu\textrm{m}$ and 66.7$\times$36.4 $\mu\textrm{m}$, respectively. After the segments of each cestode were fixed, embedding and hematoxylin-eosin dyeing on a microtome-made specimen were done. The micrographs of the semicon's aceto-carmine dyed specimen showed that Diphyllobothrium latum and Diphyllobothrium latum parvum had a centrally-located genital gland and an opened uterine pore. The yolks were observed on both sides of proglottids and had a typical rosette pattern. Yet, Diphyllobothrium latum parvum was shown smaller than Diphyllobothrium latum in the micrograph. Proglottids of Spirometra erinacei displayed that the uterus was rolled spirally more than five to seven times, and connected successively to the seminal vesicle in the cirrus sac. Shown above, this study was performed to measure the size of eggs and analyze the morphological characteristics of proglottids and provided the measurements of three types of cestodes obtained by a light microscope.

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Comparison of Determination Methods for Available-P in Soil of Plastic Film House (시설재배 토양의 유효인산 측정방법 비교)

  • Yang, Won-Seok;Kang, Seong-Soo;Kim, Ki-In;Hong, Soon-Dal
    • Korean Journal of Soil Science and Fertilizer
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    • v.39 no.3
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    • pp.163-172
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    • 2006
  • Pot experiments were conducted from 1999 to 2001 to compare the different methods of available phosphorus (P) for estimation of biomass and P uptake by tomato and cucumber grown on different soils (25 soils for tomato and 8 soils for cucumber cultivation) collected from plastic film house of Chungbuk area. Supplementary experiment was conducted to estimate the relationship among several extraction methods of available P such as P adsorption, water extractable-P, Lancaster-P, Olsen-P, Bray No 1 and No 2-P, and Mehlich 1 and 3-P for a total of 71 soils that included 33 soils collected for tomato and cucumber cultivation and 38 soils taken from other sites of plastic film house. All the extraction methods of available phosphorus except P adsorption were mutually positive correlated with r ranging from 0.81 to 0.96 while the correlation coefficient between P adsorption and other methods ranged from -0.57 to -0.80. Phosphorus uptake by tomato plant applied with no fertilizer was significantly correlated with the available P extracted by different methods except P adsorption in all the experiments showing positive correlation coefficients from 0.49 to 0.76 in April, 1999, 0.53 to 0.71 in April, 2000, and 0.59 to 0.68 in October, 2000. Consequently relative amount of P uptake by tomato plant for all the experiments also significantly correlated with available P in soils showing correlation coefficients of r=0.64~0.73 (P<0.0000001) in the order of Mehlich 1-P > Mehlich 3-P > Lancaster-P. For tomato, critical concentrations of available P in soils estimated by Cate and Nelson split method were $1700mg\;kg^{-1}$ for Mehlich 3-P, $1,050mg\;kg^{-1}$ for Mehlich 1-P, and $95mg\;kg^{-1}$ for water extractable P. Also P uptake by cucumber plant was significantly correlated with Olsen-P, water extractable P, and Bray No 2-P with r value of 0.62, 0.59, and 0.51, respectively, in soils of no fertilization.

Estimation for Red Pepper(Capsicum annum L.) Biomass by Reflectance Indices with Ground-Based Remote Sensor (지상부 원격탐사 센서의 반사율지수에 의한 고추 생체량 추정)

  • Kim, Hyun-Gu;Kang, Seong-Soo;Hong, Soon-Dal
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.2
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    • pp.79-87
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    • 2009
  • Pot experiments using sand culture were conducted in 2004 under greenhouse conditions to evaluate the effect of nitrogen deficiency on red pepper biomass. Nitrogen stress was imposed by implementing 6 levels (40% to 140%) of N in Hoagland's nutrient solution for red pepper. Canopy reflectance measurements were made with hand held spectral sensors including $GreenSeeker^{TM}$, $Crop\;Circle^{TM}$, and $Field\;Scout^{TM}$ Chlorophyll meter, and a spectroradiometer as well as Minolta SPAD-502 chlorophyll meter. Canopy reflectance and dry weight of red pepper were measured at five growth stages, the 30th, 40th, 50th, 80th and 120th day after planting(DAT). Dry weight of red pepper affected by nitrogen stress showed large differences between maximum and minimum values at the 120th DAT ranged from 48.2 to $196.6g\;plant^{-1}$, respectively. Several reflectance indices obtained from $GreenSeeker^{TM}$, $Crop\;Circle^{TM}$ and Spectroradiometer including chlorophyll readings were compared for evaluation of red pepper biomass. The reflectance indices such as rNDVI, aNDVI and gNDVI by the $Crop\;Circle^{TM}$ sensor showed the highest correlation coefficient with dry weight of red pepper at the 40th, 50th, and 80th DAT, respectively. Also these reflectance indices at the same growth station was closely correlated with dry weight, yield, and nitrogen uptake of red pepper at the 120th DAT, especially showing the best correlation coefficient at the 80th DAT. From these result, the aNDVI at the 80th DAT can significantly explain for dry weight of red pepper at the 120th DAT as well as for application level of nitrogen fertilizer. Consequently ground remote sensing as a non-destructive real-time assessment of plant nitrogen status was thought to be a useful tool for in season nitrogen management for red pepper providing both spatial and temporal information.

Dry Weight and Nitrogen Contents in Different Parts of 'Fuyu' Persimmon as Affected by Application Timing and Methods of Supplemental Nitrogen (질소 추비 시기와 방법이 '부유' 단감나무의 건물중과 질소함량 및 수체 부위별 분포에 미치는 영향)

  • Park, Doo-Sang;Choi, Seong-Tae;Kang, Seong-Mo
    • Horticultural Science & Technology
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    • v.28 no.5
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    • pp.728-734
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    • 2010
  • We studied the accumulation and partitioning of dry weight (DW) and nitrogen (N) in different parts of field-grown 'Fuyu' persimmon to elucidate that the foliar applications of supplemental N in June or September compared favorably with the traditional soil application in securing leaf area and fruit production. We also estimated the proportion of N permanently removed from the tree at the end of a growing season. Urea was applied either to leaves in June and/or September or to the soil in June and September for three consecutive years, and the trees were excavated in November for analyses. Total DW ranged from 4.2-4.8, 8.7-9.2, and 17.1-21.5 kg in a 4-, 5-, and 6-year-old tree, respectively, without statistical difference among the four treatments. Of the total DW, 3.3-10.2% was in shoots, 5.7-10.5% in leaves, 8.3-31.4% in aerial woods, 13.0-27.0% in root, and 28.0-59.3% in fruits. As the trees became more productive, DW proportion of fruits significantly affected that of the root: in 6-year-old trees, root DW accounted for only 10.6-15.8% of the tree total when fruit DW accounted for 50-60%. N contents ranged from 24.6-28.3, 48.3-53.5, and 98.3-122.6 g in a 4-, 5-, and 6-year-old trees, respectively, without statistical difference among the treatments. Of the total N, 6.2-11.5% was in shoots, 16.7-24.3% in leaves, 17.6-23.5% in aerial woods, 17.2-37.5% in roots, and 16.9-34.4% in fruits. As in DW, the increase in the proportion of N in fruits decreased in the root most significantly. Application methods for supplemental N did not affect the proportion of DW and N removed from the tree through abscising leaves and harvested fruits. Percentage of DW removal was 41 in 4- and 5-year-old trees, but it was 61 in more productive 6-year-old trees; that of N was 39, 43, and 49%, respectively. No significant changes in the contents of DW and N in field-grown trees, as well as their percentages removed from the tree at the end of the season, demonstrated that foliar application of supplemental N was as good as soil applications with much less N.

Estimation of Nitrogen Uptake and Biomass of Rice (Oryza sativa L.) Using Ground-based Remote Sensing Techniques (지상 원격측정 센서를 활용한 벼의 생체량과 질소 흡수량 추정)

  • Gong, Hyo-Young;Kang, Seong-Soo;Hong, Soon-Dal
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.5
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    • pp.779-787
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    • 2011
  • This study was conducted to evaluate the usefulness of ground-based remote sensing for the estimation of rice yield and application rate of N-fertilizer during growing season. Dongjin-1, Korean cultivar of rice was planted on May 30, 2006 and harvested on October 9, 2006. Chlorophyll content and LAI (leaf area index) were measured using Minolta SPAD-502 and AccuPAR model LP-80, respectively. Reflectance indices were determined with passive sensors using sunlight and four types of active sensors using modulated light, respectively. Reflectance indices and growth rate were measured three times from 29 days to 87 days after rice plating and at harvesting day. The result showed that values of growing characteristics and reflectance indices were highly correlated. Growing characteristics to show significant correlation with reflectance indices were in order of followings: fresh weight > N uptake > dry weight > height > No. of tiller > N content. Chlorophyll contents measured by chlorophyll meter (SPAD 502) showed high correlation with nitrogen concentration (r=$0.743^{**}$), although the correlation coefficients between remote sensing data and nitrogen concentration were higher. LAI was highly correlated with dry weight (r=$0.931^{**}$), but relationship between LAI and nitrogen concentration (r=$0.505^*$) was relatively low. The data of CC-passive sensor were negatively correlated with those of the near-infrared. NDVI correlation coefficients found more useful to identify the growth characteristics rather than data from single wavelength. Both passive sensor and active sensor were highly significantly correlated with growth characteristics. Consequently, quantifying the growth characteristics using reflectance indices of ground-based remote sensing could be a useful tool to determine the application rate of N fertilizer non-destructively and in real-time.

The Health Behavior Patterns of Some Rural Residents in Korea and Their Association with Health Status and Health Management Practice (일부 농촌주민의 건강행위유형과 건강상태 및 건강관련실태와의 관련성)

  • Kim, Young-Gab;Kang, Myung-Guen;Ryu, So-Yeon;Kim, Ki-Soon;Kang, Sung-Deuk
    • Journal of agricultural medicine and community health
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    • v.29 no.1
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    • pp.43-63
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    • 2004
  • Objectives: The purpose of this study was to classify the patterns of health behaviors of some rural residents in Korea by sub-grouping them into populations with similar patterns of diet quality, physical activity, alcohol consumption and cigarette smoking, and then to investigate the relationship between these health behavior patterns and health status or health management of them. Methods: The study subjects were 722 rural residents above 20 years old on a typical rural district in Korea, and the data used in this study was from the survey data for health planning of a health center. Study questionnaire for this survey was developed from modifying the questionnaire for 'National Nutrition and Health Study' conducted in 1998. To classify health behavior patterns, cluster analysis was conducted. And to test the association of health behavior patterns with health status or health management, multiple logistic regression analysis were conducted. Results: The results and their implications of this study were as follows: 1. We identified six health behavior typologies : 67.8% of the sample had a good diet quality but showed sedentary activity level(good diet lifestyle) and 10.9% had heavy smoking behavior(smoking lifestyle). Individuals included in fitness lifestyle cluster(6.2%) had high physical activity level and those in drinking life style(2.6%) had had mainly large amount of alcohol. Zero point six percent of sample were included in hedonic lifestyle cluster, who showed poor health behaviors in all. Those included in passive lifestyle(11.9%) had no active health promoting activities but tended to avoid risk taking health behavior such as cigarette smoking and alcohol drinking. 2. As a result of logistic regression analysis, to compare with the individuals in good diet lifestyle, the prevalence of chronic diseases of those in fitness lifestyle showed higher and that of those in smoking lifestyle, drinking lifestyle, hedonic lifestyle, passive lifestyle showed lower than them, retrospectively. 3. Adjusting with general characteristics and health status, to compare with the individuals in good diet lifestyle, the proportion of those who had good health management practices in fitness lifestyle was higher, and the proportion of those who had health check in past 2 years was lower than them, retrospectively. Conclusions: There were some differences in health behavior patterns between rural population and national population, which influenced significantly on health status and health management practice of them. We suggested that the health promotion program for them be developed with considering these points.

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Determination of the Optimum Sampling Area for the Benthic Community Study of the Songdo Tidal Flat and Youngil Bay Subtidal Sediment (송도 갯벌과 영일만 조하대 저서동물의 군집조사를 위한 적정 채집면적의 결정)

  • Koh, Chul-Hwan;Kang, Seong-Gil;Lee, Chang-Bok
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.4 no.1
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    • pp.63-70
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    • 1999
  • The optimum sampling area which can be applied to the benthic community study is estimated from large survey data in the Songdo tidal flat and subtidal zone of Youngil Bay, Korea. A total of 250 samples by 0.02 $m^2$ box corer for the benthic fauna in Songdo tidal flat and 50 samples by 0.1 $m^2$ van Veen grab in Youngil Bay were taken from the total sampling area of 5 $m^2$. It was assumed that the sampling area could contain sufficient information on sediment fauna, if cumulative number of species, ecological indices, and similarity index by cluster analysis reflect the similarity level of 75% to those found at total sampling area (5 $m^2$). A total of 56 and 60 species occurred from Songdo tidal flat and Youngil Bay, respectively. The cumulative curve of the species number ($N_{sp}$) as a function of the sampling area (A in $m^2$ ) was fitted as $N_{sp}=37.379A^{0.257}$ ($r^2=0.99$) for intertidal fauna and $N_{sp}=40.895A^{0.257}$ ($r^2=0.98$) for subtidal fauna. Based on these curves and 75% of similarity to the total sampling area (5 $m^2$), the optimum sampling area was proposed as 1.6 $m^2$ for the intertidal and 1.5 $m^2$ for the subtidal fauna. Ecological indices (species diversity, richness, evenness and dominance indices) were again calculated on the basis of species composition in differently simulated sample sizes. Changes in ecological indices with these sample sizes indicated that samplings could be done by collecting fauna from < 0.5 $m^2$-1.5 $m^2$ on the Songdo tidal flat and from < 0.5 $m^2$-1.2 $m^2$ in Youngil Bay. Changes in similarity level of all units of each simulated sample size showed that sampling area of 0.3 $m^2$ (Songdo tidal flat) and 0.6 $m^2$ (Youngil Bay) should be taken to obtain a similarity level of 75%. In conclusion, sampling area which was determined by cumulative number of species, ecological indices and similarity index by cluster analysis could be determined as 1.5 $m^2$ (0.02 $m^2$ box corer, n=75) for Songdo tidal flat and 1.2 $m^2$ (0.1 $m^2$ van Veen grab, n=12) for Youngil Bay. If these sampling areas could be covered in the field survey, population densities of seven dominant species comprising 68% of the total faunal abundance occurring on Songdo tidal flat and six species comprising 90% in Youngil Bay can be estimated at the precision level of P=0.2.

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