• Title/Summary/Keyword: 감염율

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Development of Non-Invasive Fecal PCR Assay for Detecting the Helicobacter Species Infection in Dogs (개의 Helicobacter 균속 감염 진단을 위한 비 침습적 분변 PCR 분석법)

  • Cheol-Yong Hwang;Hwa-Young Youn;Hong-Ryul Han
    • Journal of Veterinary Clinics
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    • v.19 no.3
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    • pp.295-298
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    • 2002
  • This study was conducted to develope noninvasive fecal PCR assay for detecting the Helicobacter species in dogs. From the DNA isolated from fecal samples, and a region of the 16S rRNA gene conserved among Helicobacter spp. was amplified In comparison with gastric biopsy test, the fecal PCR assay showed high specificity(100%) and sensitivity(96%). The prevalence of Helicobacter spp. infection in privately owned pet dogs in Korea detemined by the fecal PCR assay was 72.1%. the fecal PCR assay determined in this study can a new noninvasive test detecting Helicobacter spp. infection in dogs.

The Clinical Usefulness of a Repeat Urine Culture 48 Hours after Antimicrobial treatment in Anatomically Normal and Abnormal Urinary Tract Infection (소아 요로 감염증 환아의 항생제 치료 48시간 후 반복적 요 배양 검사의 임상적 유용성)

  • Park, Kyung-Hee;Yeom, Jung-Suk;Park, Ji-Suk;Park, Eun-Sil;Seo, Ji-Hyun;Lim, Jae-Young;Park, Chan-Hoo;Youn, Hee-Shang
    • Childhood Kidney Diseases
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    • v.13 no.1
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    • pp.49-55
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    • 2009
  • Purpose : We aimed to compare the frequency of positive repeat urine cultures 48 hours after antimicrobial treatment between anatomically normal and abnormal urinary tract Infection (UTI) groups to determine the potential clinical usefulness of the tests. Methods : We reviewed medical records of 930 patients under age 14, who had been admitted for UTI at Gyeongsang National University Hospital from January 1, 1998 to August 1, 2008. The eligible patients were divided into two groups the anatomically normal UTI group and the anatomically abnormal UTI group. Statistical analyses were performed with variables consisting of the sex ratio, age distribution and the frequency of positive repeat urine cultures of each group. Results : The sex ratio of the anatomically normal UTI group was M:F=1.9:1, whereas that of the anatomically abnormal UTI group was M:F=3.5:1 (P=0.019). For age distribution, it was found that the mean age of the anatomically normal UTI group was $0.82{\pm}1.83$ years, whereas that of the anatomically abnormal UTI group was $1.18{\pm}2.57$ years (P=0.113). The frequency of positive repeat urine cultures in the anatomically normal UTI group was 3/279 (1.1%), whereas that of the anatomically abnormal UTI group was 1/90 (1.1%) (P=0.675). Conclusion : We conclude that performing a repeat urine culture is not justified in terms of clinical usefulness, and it is unreasonable to use the results as an index of therapeutic success. A follow-up urine culture is unnecessary in patients with both the anatomically normal and abnormal UTI group.

An Evaluation on the Prevalence and Reinfection after Medication of Patients with Clonorchis sinensis in an Endemic Locality (간흡충 만연 일 지역에서 투약 후 유병률 및 재감염율 조사)

  • Kim, Suk-Il;Park, Jong;Kim, Ki-Soon;Yang, Ae-Hyang;Kim, Young-Lak
    • Journal of agricultural medicine and community health
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    • v.24 no.2
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    • pp.225-232
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    • 1999
  • A survey of an endemic locality at Songjung Ri, Ogok Myun, Goksung County, South Cholla Province for the prevalence of Clonorchis sinensis and Metagonimus yokogawai was primarily done on 53 residence before medication using formalin-ether concentration method of stool examination. After praziquantel medication of all the clonorchiasis patients, 64 humans consisting 82.1% of total inhibitants and including 84.9% of primarily surveyed residents were secondly surveyed after 2-year post-treatment to evaluate the prevalence and reinfection. The prevalence of clonorchiasis was decreased from 35.8% before medication to 10.9% after medication(P<0.01), indicating this disease was not satisfactorily controlled although the rate was significantly lowered. The prevalence of metagonimiasis was dropped from 17.0% to 6.3% on post-treatment. In clonorchiasis cases, sex ratio showed no difference in both pre- and post-treatment, and mean age was the sixties after medication from the forties and fifties before medication, suggesting more aged people of both sexes were infected with this fluke. The reinfection of clonorchiasis and metagonimiasis following 2-year post-treatment was 15.8% and 25.0%, respectively. The proportion of reinfection among the egg positive cases was 50.0% in clonorchiasis and 100% in metagonimiasis. These findings mean that a high fraction of clonorchiasis and metagonimiasis was reinfected with these trematodes. In conclusion, to eradicate the C. sinensis and M. yokogawai in an endemic area, it should be preceded to control the reinfection of these parasites.

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Postoperative Deep Infection after Arthroscopic Knee Surgery (슬관절 관절경 수술 후 발생한 심부 감염)

  • Kim, Key-Yong;Ha, Dong-Jun;Shim, Hyung-Nam;Seo, Seung-Suk
    • Journal of the Korean Arthroscopy Society
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    • v.11 no.1
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    • pp.20-23
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    • 2007
  • Purpose: The purpose of this study is to evaluate characteristics of a deep infection after arthroscopic knee surgery. Materials and Methods: We selected 894 patients who underwent arthroscopic knee surgery between February 1994 and August 2006. We analyzed the results of the patients with definite infection. Results: Seven cases out of the 894 knee arthroscopic surgery which was performed by one surgeon during 12 years were diagnosed as postoperative deep infection (0.9%). Infection developed in one repair case among the meniscal surgeries (1419=0.2%). There were six infection cases in intraarticular ligament reconstruction (6/343=2%); 3 in ACL surgeries (3/152), 2 in PCL surgeries (2/70) and 1 in combined cruciate ligament surgery and extra-articular reconstruction (l/26). Conclusion: Postoperative infection rate of arthroscopic knee surgery was relatively low. However an attention for the prevention of postoperative deep infection should be paid in intraarticular ligament reconstruction because of its relatively high risk of infection.

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Clinical Availability of Rapid Strep Test in Children with Group A Streptococcal Pharyngotonsilitis (A군 연쇄구균 상기도 감염에 있어 신속검사의 유용성)

  • Kim, Yeon Ho;Bae, Young Min;Cha, Sung Ho;Ma, Sang Hyuk
    • Pediatric Infection and Vaccine
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    • v.8 no.1
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    • pp.90-93
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    • 2001
  • Purpose : The accurate diagnosis and proper treatment of group A streptococal infection should be emphasized concerning about possible development of late sequelae, such as acute rheumatic fever and acute glomerulonephritis. Inadequate & improperance of antobiotics have resulted in increased number of antibiotic-resistant bacteria. We would like to know the clinical usefulness of rapid strep test compared with conventional throat culture in out-patients with acute pharyngotonsilitis. Methods : From Sep. 2000. to Jan. 2001, rapid strep test(LINK 2 Strep A, USA) & throat culture were taken from 87 patients with clinically suspect pharyngotonsilitis from Masan Fatima hospital & kyunghee university hospital. Results : Of 87 cases with pharyngitis, 39 cases proved to have group A streptococci by throat culture. The positive predictive value of rapid test was 92.3%(36 of 39 cases) and sensitivity test was 81.8%(36 of 44 cases). The specificity of rapid test was 93.0%(40 of 43 cases) and negative predictive value was 83.3%(40 of 48 cases). Conclusion : The positive predictive value & specificity of rapid strep test is high. And so, this test will give the pediatricians practical guidance of antibiotic use in patients with pharyngitis. But more efforts should be made to prevent antibiotics abuse and correct diagnosis of pharyngitis.

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Studies on the Seed Transmission of Colletotrichum spp. in Red Pepper (Capsicum annuum) (고추 탄저병균(炭疽病菌)의 종자전염(種子傳染)에 관(關)한 연구(硏究))

  • Yu, Seung Hun;Park, Jong Seong;Lee, Hyang Burm;Kim, Hong Gi
    • Korean Journal of Agricultural Science
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    • v.14 no.1
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    • pp.16-25
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    • 1987
  • Colletotrichum acutatum, C. coccodes, C. dematium and C. gloeosporioides were detected in seed samples of red pepper (Capsicum annuum). C. dematium and C. gloeosporioides were the predominant species, maximum seed infection of the species in some samples were 84% and 28%, respectively. C. acutatum and C. coccodes were recorded only in low percentages of 1-2. The blotter method proved more suitable for detecting Colletotricum spp. than the deep freezing blotter or agar plate methods. Plating of seed components showed that C. dematium and C. gloeosporioides were recovered more frequently from seed coat, and decreasing amounts of infection were observed in the endosperm and cotyledon. Seed-borne C. dematium and C. gloeosporioides caused seed rot, damping-off, seedling blight and brown discoloration of cotyledon and hypocotyl when infected seeds were sown in agar of test tube or in soil. Inoculation experiments showed that C. acutatum was pathogenic to red fruit of red pepper and C. coccodes was highly pathogenic to red fruit and weakly pathogenic to leaf of the plant. C. dematium was highly pathogenic to leaf and green fruit and C. gloeosporioides was pathogenic to not only leaf but also green and red fruits. Host range of the four seed-borne species of Colletotrichum was also investigated.

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Effect of P Levels in Nutrient solution on the Propagation of Arvuscular Mycorrhizal Funfi in Aeroponics (분무경재배에 의한 arbuscular 균근균 증식에서 양액내 인산농도의 영향)

  • Kim, Young-Ju;Jin, Seo-Yeong;Cho, Ja-Yong;Kim, Kil-Yong;Cha, Gyu-Seok;Soh, Bo-Kyoon
    • Korean Journal of Soil Science and Fertilizer
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    • v.37 no.5
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    • pp.350-355
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    • 2004
  • This experiment was carried out to screen the aeroponically grown host plants suitable for the mass propagation of arbuscular mycorrhizal fungi (AMF) inoculum and clarify the effect of P levels in nutrient solution on the growth of aeroponically grown sweet potato (Ipomoea batatas L.), AMF infection, and mass propagation of mycorrhizal spores, etc. Amount of biomass of host plant became higher, as the P levels in nutrient solutions increased from 5 to 20 M. AMF infection rates in mycorrhizal roots increased in higher P levels in nutrient solution, and decreased in lower parts of mycorrhizal roots by about 18.6-26.0%. About 586 mycorrhizal spores per 1 g fresh root were formed at 16 weeks after inoculation of mycorrhizal inoculum. Total of 830,479 mycorrhizal spores were propagated in each plot.

Evaluation of Native Soybean Collection for Resistance to Purple Blotch (수집재래종대두의 자주빛무늬병(Cercospora kikuchii)에 대한 저항성검정)

  • Oh Jeung Haing;Kwon Shin Han
    • Korean journal of applied entomology
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    • v.20 no.3 s.48
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    • pp.131-134
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    • 1981
  • Native soybean collections were evaluated to search a resistant gene source to purple blotch caused by Cercospora kikuchii. Among 467 native lines, about $28.9\%$ of the lines was less than $0.1\%$ and $13.4\%$ was over $2\%$ in natural infection of purple blotch. Natural infection seemed to be significantly associated with weather conditions at the early podding stage. A significant correlation between natural infection and purple discoloration by seed inoculation was observed and this method seemed to be effective as a preliminary screening technique for resistance to purple blotch. Most of the late maturing native soybeans showed susceptible reaction by the seed inoculation contrary to low infection under natural conditions, indicating that the low natural infection might be due to disease escaping by the late maturing instead of their genetic resistance.

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Analysis of Postnatal Acquisition Factors of the Normal Flora in Infants with Urinary Tract Infection (영아 요로감염에서 정상 세균총의 생후 획득 요인에 관한 분석)

  • Shim, Yoon-Hee;Kim, Hee-Jung;Lee, Seung-Joo
    • Childhood Kidney Diseases
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    • v.11 no.2
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    • pp.195-202
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    • 2007
  • Purpose : The normal flora, which was suggested to prevent infection, is acquired first from the birth canal and develops by dietary factors. Here presents a case-control study, aimed to evaluate the postnatal acquisition factors relating to the achievement of the normal flora in infants with urinary tract infection (UTI). Methods : 115 UTI infants, admitted at Ewha Womans University Mokdong Hospital from 2004 to 2005 and 116 age-matched control infants, who had visited well baby clinic, were evaluated. The suggested postnatal acquisition factors of the normal flora such as vaginal delivery, breast feeding, probiotics and yogurt intake and their relationship with UTI were evaluated. Results : The rate of vaginal delivery was 50%(58/l15) in UTI infants, which was not significantly different from 60%(69/116) in control infants(P>0.05). In the infants with UTI, the feeding pattern(breast milk 19%, mixed 26%, formula 55%) was significantly different from that(44%, 19%, 37%) in control infants(P<0.05). This significant difference was shown only in infants less than 6 months of age, but was not in infants over 6 months of age. The rate of probiotics intake in UTI infants was 4%(4/115), which was significantly lower than 27% (32/116) in control infants(P<0.05). The rate of regular intake of yogurt in UTI infants over 6 months of age was 27%(6/23), which was not significantly different from 35%(8/23) in control infants(P>0.05). The odds ratios of breast feeding and probiotics intake against UTI were significantly low as 0.30 (95% Cl 0.17-0.55)(P<0.01) and 0.03 (95% Cl 0.01-0.07)(P<0.01). Conclusion : The significantly lower rates of breast feeding and probiotics intake in UTI infants suggest that these dietary factors might have preventive effects in infants with UTI.

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