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Development of Non-Invasive Fecal PCR Assay for Detecting the Helicobacter Species Infection in Dogs  

Cheol-Yong Hwang (College of Veterinary Medicine, Seoul National University)
Hwa-Young Youn (College of Veterinary Medicine, Seoul National University)
Hong-Ryul Han (College of Veterinary Medicine, Seoul National University)
Publication Information
Journal of Veterinary Clinics / v.19, no.3, 2002 , pp. 295-298 More about this Journal
Abstract
This study was conducted to develope noninvasive fecal PCR assay for detecting the Helicobacter species in dogs. From the DNA isolated from fecal samples, and a region of the 16S rRNA gene conserved among Helicobacter spp. was amplified In comparison with gastric biopsy test, the fecal PCR assay showed high specificity(100%) and sensitivity(96%). The prevalence of Helicobacter spp. infection in privately owned pet dogs in Korea detemined by the fecal PCR assay was 72.1%. the fecal PCR assay determined in this study can a new noninvasive test detecting Helicobacter spp. infection in dogs.
Keywords
Helicobacter species; Fecal PCR; Prevalence; Dog;
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