Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Development of Non-Invasive Fecal PCR Assay for Detecting the Helicobacter Species Infection in Dogs

개의 Helicobacter 균속 감염 진단을 위한 비 침습적 분변 PCR 분석법

  • Cheol-Yong Hwang (College of Veterinary Medicine, Seoul National University) ;
  • Hwa-Young Youn (College of Veterinary Medicine, Seoul National University) ;
  • Hong-Ryul Han (College of Veterinary Medicine, Seoul National University)
  • Published : 2002.09.01
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Abstract

This study was conducted to develope noninvasive fecal PCR assay for detecting the Helicobacter species in dogs. From the DNA isolated from fecal samples, and a region of the 16S rRNA gene conserved among Helicobacter spp. was amplified In comparison with gastric biopsy test, the fecal PCR assay showed high specificity(100%) and sensitivity(96%). The prevalence of Helicobacter spp. infection in privately owned pet dogs in Korea detemined by the fecal PCR assay was 72.1%. the fecal PCR assay determined in this study can a new noninvasive test detecting Helicobacter spp. infection in dogs.

본 연구에서는 개의 Helicobacter 균속 감염을 비침습적 방법으로 진단하기 위해 분변을 시료로한 PCR 검사법을 확립하고 그 효능을 검정하고자 하였다. 분변 PCR 분석은 분변에서 채취된 DNA에서 Helicobacter 균속의 16S RNA의 특이적인 영역에 반응하는 primer를 이용해 수행하였다. 분변 PCR분석법에 의한 결과와 위조직 검사 법 결과를 비교해 본 결과 분변 PCR분석법은 높은 특이도 (100%)와 민감도(96%)를 나타내었다. 도한 확립된 분볍 PCR분석법을 이용해 국내 애완결들의 위내 Helicobacter 균속 감염율을 조사한 결과 감염율이 72.1%로 나타났다. 이상의 결과 본 연구에서 확립한 분변 PCR 분석법은 개의 Helicobacter 균 감염을 진단할 수 있는 새로운 비침습적 진단방법으로 이용될 수 있으리라 사료된다.

Keywords

References

  1. J Clin Microbiol v.35 Fecal PCR assay for diagnosis of Helicobacter infection in laboratory rodents Beckwith CS;Franklin CL;Hook RR;Besch-Williford CL;Riley LK
  2. Am J Vet Res v.59 Use of a $[^{13}C]$urea breath test for detection of gastric infection with Helicobacter spp. in dogs Cornetta AM;Simpson KW;Strauss-Ayali D;McDonough PL;Gleed RD
  3. J Clin Microbiol v.36 Presence of multiple Helicobacter heilmannii strains in an individual suffering from ulcers and in his two cats Dieterich C;Wiesel P;Neiger R;Blum A;Corthesy-Theulaz I.
  4. Infect Immun v.63 Helicobacter pylori-induced gastritis in the domestic cat Fox JG;Batcheder M;Marini R;Yan L;Handt L;Li X;Shames B;Hayward A;Campbell J;Murphy JC
  5. Gastroenterology v.114 Hepatic Helicobacter species identified in bile and gallbladder tissue from Chileans with chronic cholecystitis Fox JG;Dewhirst FE;Shen Z;Feng Y;Taylor NS;Paster BJ;Ericson RL;Lau CN;Correa P;Araya JC;Roa I https://doi.org/10.1016/S0016-5085(98)70589-X
  6. Vet Rec v.133 Occurrence of spiral-shaped bacteria in gastric biopsies of dogs and cats Geyer C;Colbatzky F;Lechner J;Hermanns W https://doi.org/10.1136/vr.133.1.18
  7. Vet Microbiol v.62 Transmission of canine gastric Helicobacter salomonis infection from dam to offspring and between puppies H nninen ML;Happonen I;Jalava K https://doi.org/10.1016/S0378-1135(98)00198-9
  8. Gut v.32 Gastritis due to spiral shaped bacteria other than H. pylori: clinical, hostological, and ultrastructural findings Heilmann KL;Borchard F https://doi.org/10.1136/gut.32.2.137
  9. J Comp Pathol v.112 Helicobacter-like organisms: Histological examination of gastric biopsies from dogs and cats Hermanns W;Kregel K;Breuer W;Lechner J https://doi.org/10.1016/S0021-9975(05)80083-0
  10. Dig Dis Sci v.39 Acute gastritis associated with spiral organisms from cats Lavelle JP;Landas S;Mitros FA;Conklin JL https://doi.org/10.1007/BF02087417
  11. J Clin Microbiol v.36 Detection and prevalence of Helicobacter infection in pet cats Neiger R;Dieterich C;Burnens A;Waldvogel A;Corthesy-Theulaz I;Halter, F.;Lauerburg, B.;Schmassmann, A.
  12. J Clin Microbiol v.27 Evidence for the occurrence of the same strain of Campylobacter pylori in the stomach and dental plaque Shames B;Krajden S;Fuksa M;Babida C;Penner JL
  13. J Clin Microbiol v.33 Identification of widespread Helicobacter hepaticus infection in feces in commercial mouse colonies by culture and PCR assay Shames B;Fox JG;Dewhirst F;Yan L;Shen Z;Taylor NS
  14. Lancet v.343 Caninehuman transmission of Gastrospillium hominis Tomson MA;Storey P;Greer R;Cleghorn GJ https://doi.org/10.1016/S0140-6736(94)93060-0
  15. Am J Vet Res v.19 Some observations concerning the presence of spirilla in the fundic glands of dogs and cats Weber AF;Hasa O;Sautter JH
  16. J Am Vet Med Assoc v.212 Comparison of gastric lesions in dogs and cats with and without gastric spiral organisms Yamasaki K;Suematsu H;Takahashi T