• Title/Summary/Keyword: 감마-선

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Safety Evaluation on Mutagenicity of White Layer Cake Containing Gamma-Irradiated Egg White (감마선 조사된 난백 함유 White Layer Cake의 돌연변이원성 평가)

  • 김미정;이주운;서지현;송현파;육홍선;최정미;변명우
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.7
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    • pp.1172-1175
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    • 2003
  • Mutagenicity of white layer cake including 20 kGy-gamma irradiated egg white manufactured as a research on the practical approaches of gamma irradiation for the reduction of egg allergy was evaluated by Salmonella typhimurium reversion assay (Ames test). The water-soluble and organic solvents mixture of methanol: chloroform (2 : 1)-soluble fractions of the white layer cake including 20 kGy-gamma irradiated egg white were examined in S. typhimurium TA98 and TA100. Both with and without metabolic activation, the number of revertant colonies were not increased in each extract compared with negative controls. No significant difference in the formation of the colonies was observed at the non-irradiated and 20 kGy-irradiated samples. The results indicate that there is no evidence of mutagenicity in white layer cake including 20 kGy-gamma irradiated egg white.

Responses of Transgenic Tobacco Plants Expressing Sweet Potato Peroxidases to Gamma Radiation (감마선에 대한 고구마 Peroxidase 형질전환 담배식물체의 반응)

  • 윤병욱;이행순;권석윤;김재성;곽상수
    • Korean Journal of Plant Tissue Culture
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    • v.26 no.4
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    • pp.265-269
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    • 1999
  • Transgenic tobacco plants expressing either a sweet potato anionic peroxidase (POD) (swpal) or neutral POD (swpnl) were irradiated by gamma radiation, and the gamma radiation-induced biochemical changes in antioxidant enzymes and plant growth inhibition were investigated at 30 days after treatment. Gamma radiation significantly inhibited the growth of all plants regardless of transgenic or nontransformed plants, showing a dose-dependent inhibition. In high dosage of 50 and 70 Gy, plant heights were severely retarded and new leaves does not emerged. No significant changes in antioxidant enzymes such as POD, superoxide dismutase and catalase were observed in all plants regardless of irradiation dosages ranging from 10 to 50 Gy. These results suggest that sweet potato PODs may be not involved in the protection against the oxidative stress induced by gamma radiation.

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Effect of Gamma Irradiation on the Microbiological and Organoleptic Qualities of Salted Sea Mustard (Undaria pinnatifida) (염장미역의 미생물적, 관능적 품질에 대한 감마선 조사의 영향)

  • 변명우;권중호;이수정;남상명;조한옥
    • Journal of Food Hygiene and Safety
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    • v.6 no.3
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    • pp.157-163
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    • 1991
  • Salted sea mustard (Undaria pinnatifida) was irradiated (0, 2,4 kGy) and stored for 6 months at $10{\pm}1^{\circ}C\;and\;30{\pm}2^{\circ}C$, respectively. Quality deterioration of stored salted sea mustard was observed to closely relate with the growth of halophilic bacteria and yeast. Gamma irradiation with 2 to 4 kGy doses reduced initial microbial loads of salted sea mustard by 1 to 2 log orders, but had little influence on the propagation during storage. Organoleptic characteristics of the sample showed no signifiant difference between nonirradiated control and irradiated samples during storage. Thus, gamma irradiation was little effective for improving the microbiological and organoleptic qualities of salted sea mustard associated with its storage stability.

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Enrichment Measurement of Nuclear Materials by Passive Gamma-ray Analysis (수동적 감마선분석에 의한 핵물질 농축도 측정)

  • Hong, Jong-Sook;Cha, Hong-Ryul;Park, Hyoung-Nae;Lee, Byung-Doo;Park, Ho-Joon
    • Nuclear Engineering and Technology
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    • v.23 no.2
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    • pp.233-240
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    • 1991
  • U-235 enrichment has been measured non-destructively by passive gamma-ray pulse height analysis. Measurement source is 185.7 keV gamma-ray which is emitted from uranium sample during alpha decay of U-235 in it. Factors influencing the measurement such as sample composition, attenuation effect of container wall, collimation effect and counting efficiency were evaluated. Under the optimized counting system, the measured relative errors were~8%, ~8% and~1% from Tag values at 95% confidence level for depleted UF$_{6}$ cylinders, depleted UO$_2$powder, and natural UO$_2$powder respectively.

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Complete genome sequence of Spirosoma montaniterrae DY10T isolated from gamma-ray irradiated soil (감마선 조사된 토양에서 분리된 박테리아 Spirosoma montaniterrae DY10T 의 완전한 게놈 서열)

  • Srinivasan, Sathiyaraj;Kang, Myung-Suk;Kim, Myung Kyum
    • Korean Journal of Microbiology
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    • v.53 no.1
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    • pp.61-63
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    • 2017
  • A Gram-negative, yellow-pigmented, long-rod shaped bacterium Spirosoma montaniterrae $DY10^T$ was isolated from a soil sample collected at Mt. Deogyusan, Jeonbuk Province, Republic of Korea. Cells showed extreme gamma radiation resistance with the $D_{10}$ value of 12 KGy. The complete genome sequence of strain $DY10^T$ is consist of a circular chromosome (5,797,678 bp) encoding 5,116 genes, 9 rRNA genes and 39 tRNA genes. The genomic features contain the key enzymes for gamma and UVC radiation.

Expression of Inhibin in the Whole-body γ-irradiated Mouse Ovary (감마선이 조사된 미성숙 생쥐 난소 내 인히빈의 발현 변화)

  • Kim, Sang Soo;Lee, Chang Joo;Yoon, Hyun-Tae;Yoon, Yong-Dal
    • Clinical and Experimental Reproductive Medicine
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    • v.33 no.1
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    • pp.35-35
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    • 2006
  • Objective: The purposes of the present study were to investigate the effect of ${\gamma}$-radiation on the expression of inhibin-${\alpha}$ proteins and genes for inhibin ${\alpha}$, ${\beta}A$, and ${\beta}B$ in the ovary. Methods: Immature mice were whole-body ${\gamma}$-irradiated with 25% of a lethal dose. At time 0, 3, 6, 12, and 24 hours after the irradiation, the ovaries were collected and used for immunohistochemistry for inhibin-${\alpha}$, and RT_PCR for inhibin-${\alpha}$, ${\beta}A$, and ${\beta}B$. Results: The expression of the immunoreactive inhibins-${\alpha}$ was maintained at 12 hours post-irradiation and reduced thereafter. The expression of inhibin-${\alpha}$ mRNA was significantly increased with the time after the irradiation. However there were no significant changes in the expression of ${\beta}A$ and ${\beta}B$ mRNAs. Conclusion: It might be thought that inhibin acts as one of the regulatory factors in the ${\gamma}$-radiation-induced follicular atresia in mice

Genotoxicological Safety of Gamma-Irradiated Salted and Fermented Anchovy Sauce (감마선 조사된 멸치액젓의 유전독성학적 안전성 평가)

  • 육홍선;차보숙;김동호;이주운;변명우
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.7
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    • pp.1192-1200
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    • 2004
  • Gamma irradiations at 5 or 10 kGy were applied to salted and fermented anchovy sauce, for improving the hygiene Quality and evaluating the genotoxicological safety. In vitro genotoxicological safety of irradiated sauces was evaluated by Salmonella Typhimurium (TA98, TA100, TAI535 and TAI537) and E. coli WP2 uvrA, reversion assay, SOS chromotest (Escherichia coli PQ37), and chromosome aberration test (Chinese hamster lung fibroblast cells) in the absence or presence of an exogenous metabolizing system (S9 mix). The gamma-irradiated samples were not significantly different from nonirradiated-control for three in vitro tests (p<0.05). :In vivo micronucleus test using ICR mice (male) was not significantly different from the control at p<0.05. The salted and fermented anchovy sauce exposed to 5 or 10 kGy-gamma ray revealed negative results in these three in vitro mutagenetic tests and in vivo micronucleus test upto 50,000 $\mu$g/plate, respectively. The results indicated that 5 or 10 kGy gamma-irradiated salted and fermented anchovy sauces did not show any mutagenicity.

Induction of Resistance against Phytophthora Blight of Pepper by Low Dose Gamma Ray Radiation (저선량 감마선 조사에 의한 고추 역병 저항성 유기)

  • 김재성;이은경;송정영;김홍기;이영복
    • Korean Journal of Environmental Biology
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    • v.18 no.1
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    • pp.47-51
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    • 2000
  • Phytophthora blight of pepper, which is caused by Phytophthora capsici Leonian, is not only the most destructive disease worldwide, but also difficult to control effectively. It has been needed to have new trials for effective control to the disease. We employed radiation hormesis of gamma ray as the new trial in the control strategy. Two cultivars, Kwangbok and Dabok, were used to analyse whether gamma ray radiation can induce disease resistance. The germination rate of pepper seeds was significantly enhanced by the radiation at all dose levels. Stimulatory effect for resistance induction was found to differ between cultivars. It was confirmed that the remarkable effect was induced in Dabok and depended on radiation dosage. Disease resistance at 4 Gy was much higher than that of control. On the other hand, no detectable induction effect for resistance was observed in Kwangbok which was moderate resistant cultivar to gamma ray radiation. [Hormesis, Gamma ray, Pepper, Phytophthora blight, Resistance induction].

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The Effect of Red Ginseng Extract on Superoxide Dismutase Activity in the Kidney of Gamma-ray Irradiated Mice (홍삼 투여가 방사선에 조사된 생쥐 신장의 Superoxide Dismutase에 미치는 영향)

  • Park, Yong-Soon
    • Journal of radiological science and technology
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    • v.15 no.1
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    • pp.123-130
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    • 1992
  • This study was prepared to observe the change of enzyme activities in kidney treated with red ginseng extract in the gamma ray irradiated mice. Determine the activity of SOD, peroxidase, catalase in the kidney a period of 1 day, 2 day, 3 day, 4 day, 5 day after a saline injection or injection of red ginseng extract or gamma ray irradiatied group into four classify. The activity SOD and catalase showed a tendency to increase and recovery at the early state but pay no regard. Wherease, the activity of peroxide restored and increased pay regard. A physiological saline injection group after gamma ray irradiation showed a tendency to diminish after remakable increase of activity of SOD, peroxidase and catalase than control group. Injection group of red ginseng extract after gamma ray irradiation observed rapid recovery on activity of SOD, peroxidase, catalase than a saline injection group. Experimental result suggested that injection of red ginseng extract after irradiation have the recovery effect on the changed of activity of SOD, peroxidase and catalase against radiation injury.

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Measurement of Gamma ray Spectrum for the 27Al(p,3p+n)24Na Nuclear Reaction by using 100 MeV Proton Acceleration System (100 MeV 양성자가속기를 이용한 27Al(p,3p+n)24Na 핵반응에 대한 감마선 스펙트럼 측정)

  • Lee, Samyol
    • Journal of the Korean Society of Radiology
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    • v.9 no.1
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    • pp.55-59
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    • 2015
  • Research about the proton nuclear reaction is actively achieving on the proton therapy including material development of fusion reactor. The proton induced gamma ray energy(2754, 1386 keV) spectrum of 27Al(p,3p+n)24Na reaction was measured with 100 MeV high energy proton beam. The proton beam in the experiment was derived from 100 MeV proton linear accelerator in the KOMAC. We measured the gamma ray intensity ratio of the decay level from the energy spectrum. The previous results have been compared with the current result. Strength of measured gamma rays will provide very important information though decide high energy gamma radiation detection efficiency.