• YAKHAK HOEJI
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• v.27 no.4
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• pp.263-270
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• 1983

Effective BuOH fraction was extracted from Puerariae radix (Pueraria thunbergiana), and several pharmacological activities were examined. From several pharmacological examinations, it was found that BuOH fraction has an antipyretic activity against typhoid vaccine pyrexia in rabbit, a papaverine-like antispasmodic activity on isolated ileum and jejunum of rat, an analgesic effects on mice treated by 0.7% HAc, and an anti-inflammatory activity of carrageenin edema in rat. It was found that BuOH fraction has detoxication effects on acute and subacute toxic rat induced by alcohol in consequence of biochemical tests of serum. Therefore it was considered that BuOH fraction has preventive and therapeutic effects in alcohol intoxication.

• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.1
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• pp.25-31
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• 1997

Antioxidative activity of daidzin and puerarin isolated from Puerariae radix against oxidation of low density lipoprotein(LDL) was investigated. The concentration of daidzin at 100$\mu\textrm{g}$/$m\ell$ and puerarin at 60$\mu\textrm{g}$/$m\ell$ inhibited Cu$^{2+}$-mediated oxidation of LDL almost completely. The electrophoretic mobility of oxidized LDL by addition of daidzin(100$\mu\textrm{g}$/$m\ell$) and puerarin(60$\mu\textrm{g}$/$m\ell$) was faster than that of native LDL, but slower than that of oxidized LDL. The oxidized LDL induced by J774 or macrophage was inhibited strongly in the presence of 100$\mu\textrm{g}$/$m\ell$ daidzin and 60$\mu\textrm{g}$/$m\ell$ Puerarin. The formation of conjugated dienes in the oxidized LDL was strongly inhibited by 100$\mu\textrm{g}$/$m\ell$ daidzin and 60$\mu\textrm{g}$/$m\ell$ puerarin.n.

• The Korea Journal of Herbology
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• v.23 no.4
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• pp.171-177
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• 2008

Objectives: This study presents a reversed-phase high-performance liquid chromatography- pulsed amperometric detection(RP-HPLC-PAD) method for the determination of puerarin and daidzin in Puerariae Radix extract and Chinese medicinal preparations. Methods: Chromatographic separation was performed using a 10% acetonitrile with a reversed-phase column(Unison UK-C18, $100mm{\times}2.0mm$ I.D.; $3{\mu}m$). The analyses were detected by pulsed amperometric detector(PAD) in alkaline conditions by combining with post-column NaOH solution. Geniposide was used as an internal standard. Results: The limit of detection(S/N=3) and the limit of quantification(S/N=10) were 0.025 ng, 0.075 ng for puerarin, and 0.05 ng, 0.15 ng for daidzin, respectively. The intra- and inter-day precisions(RSDs) were less than 6.5% and average recoveries of puerarin were 99.7-101.3% and those of daidzin were 101.0-102.8%. Conclusions: According to above results, we developed a determination method for puerarin and daidzin in Puerariae Radix with high sensitivity and selectivitely.

• Food Science and Preservation
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• v.12 no.3
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• pp.282-286
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• 2005

This study was to investigate the content of total phenolic compounds, electron donating ability, nitrate-scavenging effect, and angiotensin converting enzyme(ACE) inhibition effect of medicinal plant liquors prepared from Cnidium officinale, Angelica gigas, Pueraria thunbergiana(root), Pueraria thunbergiana (flower), and Glycyrrhiza uralensis. Physicochemical characteristics of the medicinal plant liquors are as follows: pH $5.65{\sim}6.36$; reducing sugar, $0.13{\sim}0.45\%$. The highest value of total phenolic compounds was found in liquor prepared with Pueraria thunbergiana(root) as $23.9{\sim}54.3\;mg\%$. The electron donating ability of liquor prepared with Pueraria thunbergiana showed the highest value($67.4{\sim}85.3\%$) among the liquors, and its nitrite scavenging ability($24.56{\sim}78.3\%$) showed the highest value than those of other medicianal plant liquors. ACE inhibitory activity showed the highest value in liquor prepared with Cnidium officinale.

• Korean Journal of Oriental Medicine
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• v.11 no.2
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• pp.167-178
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• 2005

As a part of the quality control of herbal prescriptions which has been used for diabetes and related diseases, a reversed-phase liquid chromatographic method was developed for the simultaneous quantification of the three marker compounds, puerarin (Puerariae Radix), glycyrrhizin (Glycyrrhizae Radix), schizandrin (Schizandrae Fructus) in Oc Chun San. The HPLC analysis method was validated for parameters such as linearity, Limits of Detection(LOD), quantification(LOQ), repeatability, stability and recovery.

• Korean Journal of Oriental Medicine
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• v.18 no.2
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• pp.91-100
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• 2012

Indications of Galgeunhaegi-tang(葛根解肌湯) for Taeeum-type on "Donguisusebowon" are small. Therefore, indications of Galgeunhaegi-tang for Taeeum-type need to be extended for broader clinical use. There would be some methods to do that, and we could review "Dongeuibogam" that Four-Constitution Medicine formulas are based on. We reviewed Galgeunhaegi-tang, which is the origin of Galgeunhaegi-tang, and their modified formula(加減方) on "Dongeuibogam". As a result, we supposed 10 indications of non-classical formula(後世方) Galgeunhaegi-tang as possible indications of Galgeunhaegi-tang for Taeeum-type.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2007.05a
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• pp.577-579
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• 2007

탕액으로 제조된 생약제제 4개 제품의 중금속의 농도는 허용치보다 낮게 검출이 되었으며, 맥문동탕의 경우 As의 농도는 0.048ppm, Cd의 농도는 0.0001ppm, Pb의 농도는 0.002ppm, Hg의 농도는 0.003ppm으로 측정되었으며, 갈근탕의 경우 As의 농도는 0.05ppm, Cd의 농도는 0.0002ppm, Pb의 농도는 0.003ppm, Hg의 농도는 0.003ppm으로 측정되었고, 쌍화탕의 경우 As의 농도는 0.063ppm, Cd의 농도는 0.001ppm, Pb의 농도는 0.004ppm, Hg의 농도는 0.002ppm으로 측정되었으며 20전 대보탕의 경우 As의 농도는 0.049ppm, Cd의 농도는 0.001ppm, Pb의 농도는 0.0185ppm, Hg의 농도는 0.003ppm으로 측정되었다.

• Journal of Sasang Constitutional Medicine
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• v.2 no.1
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• pp.123-134
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• 1990

In order to investigate experimentally the clinical effects of Taeuminkalkunhaeki-tang (太陰人葛根解肌湯) that was prescribed to cure the Kansuyolriyolbyong (肝受熱裏熱病) of Taeumin, the author experimented various activities of mixed extract from Taeuminkalkunhaeki-tang by the methods prescribed in the experimental part. The results of the studies were summarized as follows: 1. Sedative effects such as malfunction of the spontaneous motor activity in the wheel cage method experiment and muscle relaxation in the rotor rod method experiment were noted. 2. In mice, the prolongation of the duration of hypnosis Induced by thiopental-Na was noted. 3. In the acetic acid method experiment and the pressing hind paw method experiment, analgesic effects were noted. 4. Inhibitory effects were noted on the convulsions incuced by strychine and picrotoxin. 5. The expansion of blood vessels by relaxation of smooth muscle was noted. According to the above results, effects based on oriental medical references approximate to the actual experimental results.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.20 no.3
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• pp.243-250
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• 2007

Trigeminal neuralgia is nerve systematic disease appearing in the distribution scope of trigeminal nerve. It's characterized by extreme pain accompanying with a repeated and simultaneous fit from several seconds to 1-2 minutes. The oriental medical name of trigeminal neuralgia is generally Dootong, Doopoong, Myuntong, Pyundootong, Pyundoopoong, and Myuntong is the nearest in Occidental medicine. So I study for general prescription of Trigeminal neuralgia and point out Galgunhaegui-tang(葛根解肌湯). Trigeminal neuralgia patients were given Galgunhaegui-tang. After taking Galgunhaegui-tang, the patients's Trigeminal neuralgia was improved.

• Korean Journal of Oriental Medicine
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• v.9 no.1
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• pp.123-126
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• 2003

Advanced glycation end products(AGEs) are largly involved in the pathogenesis of diabetic complications. It is obvious that inhibition of AGEs formation is important in preventing the occurrence and progression of diabetic complications. Therefore, to seek possible AGEs inhibitors in herbal medicines, unprocessed - und processed Puerariae Radix were tested. The inhibitory effect on AGEs formation was slightly increased through processing. Unprocessed-, processed Puerariae Radix and puerarin showed potential inhibitory action than that of positive control, amino guanidine HCI.