The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Determination Method of Puerarin and Daidzin from Puerariae Radix by Reversed-Phase HPLC with Pulsed Amperometric Detection

RP-HPLC-PAD를 이용한 갈근(葛根)과 갈근(葛根) 함유 처방의 Puerarin과 Daidzin 분석

  • Chiao, Chen-Ying (Dept. of Oriental Pharmaceutical Sciences, Kyung Hee University) ;
  • Kwon, Ha-Jeong (Dept. of Oriental Pharmaceutical Sciences, Kyung Hee University) ;
  • Jeong, Ji-Seon (Dept. of Oriental Pharmaceutical Sciences, Kyung Hee University) ;
  • Lee, Je-Hyun (Korean Medicine Research Center, Dongguk University) ;
  • Hong, Seon-Pyo (Dept. of Oriental Pharmaceutical Sciences, Kyung Hee University)
  • 초진영 (경희대학교 한약학과 천연물 실험실) ;
  • 권하정 (경희대학교 한약학과 천연물 실험실) ;
  • 정지선 (경희대학교 한약학과 천연물 실험실) ;
  • 이제현 (동국대학교 한의과대학 한의학과 본초학교실) ;
  • 홍선표 (경희대학교 한약학과 천연물 실험실)
  • Published : 2008.12.30
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Abstract

Objectives: This study presents a reversed-phase high-performance liquid chromatography- pulsed amperometric detection(RP-HPLC-PAD) method for the determination of puerarin and daidzin in Puerariae Radix extract and Chinese medicinal preparations. Methods: Chromatographic separation was performed using a 10% acetonitrile with a reversed-phase column(Unison UK-C18, $100mm{\times}2.0mm$ I.D.; $3{\mu}m$). The analyses were detected by pulsed amperometric detector(PAD) in alkaline conditions by combining with post-column NaOH solution. Geniposide was used as an internal standard. Results: The limit of detection(S/N=3) and the limit of quantification(S/N=10) were 0.025 ng, 0.075 ng for puerarin, and 0.05 ng, 0.15 ng for daidzin, respectively. The intra- and inter-day precisions(RSDs) were less than 6.5% and average recoveries of puerarin were 99.7-101.3% and those of daidzin were 101.0-102.8%. Conclusions: According to above results, we developed a determination method for puerarin and daidzin in Puerariae Radix with high sensitivity and selectivitely.

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