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A Practical Method to Quantify Very Low Fluxes of Nitrous Oxide from a Rice Paddy (벼논에서 미량 아산화질소 플럭스의 정량을 위한 실용적 방법)

  • Okjung, Ju;Namgoo, Kang;Hoseup, Soh;Jung-Soo, Park
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.24 no.4
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    • pp.285-294
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    • 2022
  • In order to accurately calculate greenhouse gas emissions in the agricultural field, Korea has been developing national-specific emission factors through direct measurement of gas fluxes using the closed-chamber method. In the rice paddy, only national-specific emission factors for methane (CH4) have been developed. It is thus necessary to develop those for nitrous oxide (N2O) affected by the application of nitrogen fertilizer. However, since the concentration of N2O emission from rice cultivation is very low, the QA/QC methods such as method detection and practical quantification limits are important. In this study, N2O emission from a rice paddy was evaluated affected by the amount of nitrogen fertilizer, by taking into account both method detection and practical quantification limits for N2O concentration. The N2O emission from a rice paddy soils affected by the nitrogen fertilizer application was estimated in the following order. The method detection limit (MDL) of N2O concentration was calculated at 95% confidence level based on the pooled standard deviation of concentration data sets using a standard gas with 98 nmol mol-1 N2O 10 times for 3 days. The practical quantification limit (PQL) of the N2O concentration is estimated by multiplying 10 to the pooled standard deviation. For the N2O flux data measured during the rice cultivation period in 2021, the MDL and PQL of N2O concentration were 18 nmol mol-1 and 87 nmol mol-1, respectively. The measured values above the PQL were merely about 12% of the total data. The cumulative N2O emission estimated based on the MDL and PQL was higher than the cumulative emission without nitrogen fertilizer application. This research would contribute to improving the reliability in quantification of the N2O flux data for accurate estimates of greenhouse gas emissions and uncertainties.

Assessment of Region Specific Angstrom-Prescott Coefficients on Uncertainties of Crop Yield Estimates using CERES-Rice Model (작물모형 입력자료용 일사량 추정을 위한 지역 특이적 AP 계수 평가)

  • Young Sang, Joh;Jaemin, Jung;Shinwoo, Hyun;Kwang Soo, Kim
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.24 no.4
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    • pp.256-266
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    • 2022
  • Empirical models including the Angstrom-Prescott (AP) model have been used to estimate solar radiation at sites, which would support a wide use of crop models. The objective of this study was to estimate two sets of solar radiation estimates using the AP coefficients derived for climate zone (APFrere) and specific site (APChoi), respectively. The daily solar radiation was estimated at 18 sites in Korea where long-term measurements of solar radiation were available. In the present study, daily solar radiation and sunshine duration were collected for the period from 2012 to 2021. Daily weather data including maximum and minimum temperatures and rainfall were also obtained to prepare input data to a process-based crop model, CERES-Rice model included in Decision Support System for Agrotechnology Transfer (DSSAT). It was found that the daily estimates of solar radiation using the climate zone specific coefficient, SFrere, had significantly less error than those using site-specific coefficients SChoi (p<0.05). The cumulative values of SFrere for the period from march to September also had less error at 55% of study sites than those of SChoi. Still, the use of SFrere and SChoi as inputs to the CERES-Rice model resulted in slight differences between the outcomes of crop growth simulations, which had no significant difference between these outputs. These results suggested that the AP coefficients for the temperate climate zone would be preferable for the estimation of solar radiation. This merits further evaluation studies to compare the AP model with other sophisticated approaches such as models based on satellite data.

Risk Assessment of Heavy Metals Migrated from Plastic Food Utensils, Containers, and Packaging Distributed in Korea (국내 유통 식품용 플라스틱 기구 및 용기, 포장의 중금속 위해도 평가)

  • Kyung Youn, Lee;Hyung Soo, Kim;Dae Yong, Jang;Ye Ji, Koo;Seung Ha, Lee;Hye Bin, Yeo;Ji Su, Yoon;Kyung-Min, Lim;Jaeyun, Choi
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.28 no.3
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    • pp.175-182
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    • 2022
  • Heavy metals can be intentionally or unintentionally introduced into plastic food utensils, containers, and packaging (PFUCP) as additives or contaminants, which can be ingested with food by humans. Here, seven-heavy metals (lead, cadmium, nickel, chromium, antimony, copper, and manganese) with toxicity concerns were selected, and risk assessment was done by establishing their migration from 137 PFUCP products made of 16 materials distributed in Korea. Migration of heavy metals was examined by applying 4% acetic acid as a food simulant (70℃, 30 minutes) to the PFUCP products. Inductively coupled plasma mass spectrometry (ICP-MS) was employed for the analysis of migrated heavy metals, and the reliability of quantitative results was confirmed by checking linearity, LOD, LOQ, recovery, precision, and expanded uncertainty. As a result of monitoring, heavy metals were detected at a level of non-detection to 8.76 ± 11.87 ㎍/L and most of the heavy metals investigated were only detected at trace amounts of less than 1 ㎍/L on average. However, antimony migrated from PET products was significantly higher than other groups. Risk assessment revealed that all the heavy metals investigated were safe with a margin of exposure above 311. Collectively, we demonstrated that heavy metals migrated from PFUCP products distributed in Korea appear to be within the safe range.

Antioxidative Effects of Tenebrio molitor Larvae Extract Against Oxidative Stress in ARPE-19 Cells (ARPE-19 세포에서 산화적 스트레스에 대한 갈색거저리 추출물의 항산화 효과)

  • Bong Sun, Kim;Ra-Yeong, Choi;Eu-Jin, Ban;Joon Ha, Lee;In-Woo, Kim;Minchul, Seo
    • Journal of Life Science
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    • v.32 no.11
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    • pp.865-871
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    • 2022
  • Tenebrio molitor larvae is well known as edible insect. Then, although it has been widely studied that Tenebrio molitor larvae has various bioactive functions such as antioxidant, anti-wrinkle, and anticancer. Nevertheless, antioxidant effects of Tenebrio molitor larvae water extract (TMH) has not been well described in Adult Retina Pigment Epithelial cell line (ARPE-19). In this study, we demonstrated that antioxidant effects of TMH against H2O2-induced oxidative stress in ARPE-19. Thus, we selected for our studies and performed a series of dose-response assay to determine the working concentration that lead to a consistent and high degree of cytotoxicity, which we defined as the level of H2O2 that killed 40% of the ARPE-19 cells. ARPE-19 cells were pre-treated with various concentrations of TMH (0.1 up to 2 mg/ml) before exposure to 300 µM H2O2. As we expected, TMH effectively prevented ARPE-19 cells from 300 µM H2O2-induced cell death in a dose-dependent manner. Furthermore, TMH inhibited the phosphorylation of mitogen-activated protein kinases (MAPKs) such as extracellular signal regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38. Overall, the inhibitory effects of TMH on H2O2-induced apoptosis and oxidative stress were associated with the protection cleaved caspase-3, Bax, Bcl-2, and HO-1. The TMH suppressed H2O2-induced cell membrane leakage and oxidative stress in ARPE-19 cells. Thus, these results suggest that the TMH plays an important role in antioxidant effect in ARPE-19.

The Demands and Awareness of Pediatric Dentists on the Korean National Health Insurance System (국민건강보험에 대한 소아치과 의사들의 요구와 인식 조사 )

  • Yunhyeong, Kim;Yongkwon, Chae;Koeun, Lee;Misun, Kim;Ok Hyung, Nam;Sungchul, Choi;Hyoseol, Lee
    • Journal of the korean academy of Pediatric Dentistry
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    • v.49 no.3
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    • pp.285-299
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    • 2022
  • The aim of this study is to investigate the awareness and perception of pediatric dentists regarding dental health insurance (DHI) system in Korea. Online surveys were sent to the members of the Korean Academy of Pediatric Dentistry (KAPD), and the responses were collected. The survey, consisting of 41 questions, was subdivided into 'general background of the respondents', 'the awareness on national health insurance (NHI) and DHI', 'the awareness on DHI pertaining to pediatric dentistry', 'issues that need improvement on DHI pertaining to pediatric dentistry', 'DHI claims', and 'the roles of KAPD in DHI'. In total, 302 responded, with a response rate of 28.9%. Excluding 2 questionnaires with insufficient answers, a total of 300 survey results were analyzed. According to the analysis, pediatric dentists thought that the coverage of DHI was not as sufficient compared to that of NHI, but were expecting its scope to broaden in the future. The satisfaction rate was higher in pit and fissure sealant and composite resin filling than in caries-detecting quantitative light-induced fluorescence. Pediatric dentists considered permanent tooth composite resin filling (65.7%) needed an increase in insurance copayment, while topical fluoride application (74.7%) to require insurance coverage. DHI claims were generally handled by dental hygienists and/or nursing assistants. Approximately half of the respondents answered that they have had experiences on appealing for insurance denials. Lastly, pediatric dentists generally had a positive attitude towards providing information for the DHI. This study is expected to be used as a sound dataset for the DHI policy development concerning pediatric dentistry.

A study on the rock mass classification in boreholes for a tunnel design using machine learning algorithms (머신러닝 기법을 활용한 터널 설계 시 시추공 내 암반분류에 관한 연구)

  • Lee, Je-Kyum;Choi, Won-Hyuk;Kim, Yangkyun;Lee, Sean Seungwon
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.23 no.6
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    • pp.469-484
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    • 2021
  • Rock mass classification results have a great influence on construction schedule and budget as well as tunnel stability in tunnel design. A total of 3,526 tunnels have been constructed in Korea and the associated techniques in tunnel design and construction have been continuously developed, however, not many studies have been performed on how to assess rock mass quality and grade more accurately. Thus, numerous cases show big differences in the results according to inspectors' experience and judgement. Hence, this study aims to suggest a more reliable rock mass classification (RMR) model using machine learning algorithms, which is surging in availability, through the analyses based on various rock and rock mass information collected from boring investigations. For this, 11 learning parameters (depth, rock type, RQD, electrical resistivity, UCS, Vp, Vs, Young's modulus, unit weight, Poisson's ratio, RMR) from 13 local tunnel cases were selected, 337 learning data sets as well as 60 test data sets were prepared, and 6 machine learning algorithms (DT, SVM, ANN, PCA & ANN, RF, XGBoost) were tested for various hyperparameters for each algorithm. The results show that the mean absolute errors in RMR value from five algorithms except Decision Tree were less than 8 and a Support Vector Machine model is the best model. The applicability of the model, established through this study, was confirmed and this prediction model can be applied for more reliable rock mass classification when additional various data is continuously cumulated.

The Induction of ROS-dependent Autophagy by Particulate Matter 2.5 and Hydrogen Peroxide in Human Lung Epithelial A549 Cells (미세먼지와 산화적 스트레스에 의한 인간 폐 상피 A549 세포에의 ROS 의존적 자가포식 유도)

  • Park, Beom Su;Kim, Da Hye;Hwangbo, Hyun;Lee, Hyesook;Hong, Su Hyun;Cheong, Jaehun;Choi, Yung Hyun
    • Journal of Life Science
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    • v.32 no.4
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    • pp.310-317
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    • 2022
  • Recently, interest in the harmful factors of particulate matter (PM), a major component of air pollution, has been increasing. In particular, PM2.5 with a diameter of less than 2.5 ㎛ is well known to induce oxidative stress accompanied by autophagy in human lung epithelial cells. However, studies on whether PM2.5 increases autophagy under oxidative stress and whether this process is reactive oxygen species (ROS)-dependent are insufficient. Therefore, in this study, we investigated whether PM2.5 promotes autophagy through the generation of ROS in human alveolar epithelial A594 cells. According to our results, cells co-treated with PM2.5 and hydrogen peroxide (H2O2) showed a lower cell viability than cells treated with each alone, which was associated with increased total and mitochondrial ROS production. The co-treatment of PM2.5 and H2O2 also increased autophagy induction, which was confirmed through Cyto-ID staining, and the expression of autophagy biomarker proteins increased. However, when ROS generation was artificially blocked by N-acetyl-L-cysteine pretreatment, the reduction in cell viability and induction of autophagy by PM2.5 and H2O2 co-treatment were markedly attenuated. Therefore, the present results suggest that PM2.5-induced ROS generation may play a critical role in autophagy induction in A549 cells.

A Study on the Experience of Photo graphic Activity of the Middle-Class Men in Their 50s: Based on the Perspective of Cultural Capital Theory (50대 중산층 남성들의 사진 활동 이야기 - 문화자본론의 관점에서 -)

  • Lee, Ye Ji
    • Korean Association of Arts Management
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    • no.58
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    • pp.5-47
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    • 2021
  • This paper is a story about five middle-aged men in their 50s who suddenly began their photographic activities as they reached middle age. In the perspective of Borudieu's cultural capital theory, this study observes five men in their 50s by implementing in-depth interviews about the motivation behind taking photographs, the experience of photography activities, and the rewards of these activities. The theory has undergone a theoretical revision with the criticism that factors other than the class can be influential. Based on these ideas, I have proceeded my study by preferentially grasping the notion of the 'field' in accordance with the specific history of Korean society. Therefore, this study sought to more specifically understand the various photographic activities of middle-class men in their 50s by referring Coskuner-Balli and Thompson's argument(2013), which revised 2018's cultural captial theory and proposed the concept of 'subordinate cultural capital' and 'leisure capital' who proposed by Backlund, E. A. & Kuentzel, W. F.(2013). As a middle-class men in their 50s, research participants have grown up and worked in a social atmosphere where economic capital is recognized as an individual's ability. However, they are faced with the value that the knowledge and taste towards culture and arts is one's identity. In addition to the subjective deprivation that arises from this situation, the lifespan characteristic of their age that it is on the brink of the old age appeared to have influenced them to put their psychological motivation immediately into practice. Economic capital was the main conversion terms to move form interest to practice, which includes 'time' as a resource as well as money. With the cultural practices being expanded since their creation of photographs, the reason that these expansions can be maintained more actively lies in their identity as 'cultural artist' that is consolidated in new relationships in the sharing of photographic activities. In this way, photographic activities grant a symbolic status of 'a middle-aged man who actively builds and expresses his identity' through the conversion of accumulating cultural capital and the conversion into social capital. Furthermore, the recognized scope of the symbolic capital acquired by the research participants is in the domain of the private life that is family and acquaintance. Especially, they were gaining a great psychological reward from their children's recognition that they are not just a 'breadwinner' but 'dad who cultivates himself with a culture and arts'. Accordingly, by considering that 'generation' other than class can be a meaningful discussion point when understanding Korea society from the perspective of cultural theory, this study is meaningful that a more flexible understanding of cultural theory can give a glimpse into the possibility of a more specific and diverse approach that will arise in the discussion of culture and arts education.

Feeding Habits of the Sandfish, Arctoscopus japonicus in the Coastal Waters of East Sea, Korea (한국 동해 연안에 출현하는 도루묵(Arctoscopus japonicus)의 식성)

  • Jin, Suyeon;Kim, Do-Gyun;Seong, Gi Chang;Kang, Da Yeon;Lee, Ju Eun;Park, Hyun-sol;Yang, Hye-jin;Soh, Ho Young;Baeck, Gun Wook
    • Korean Journal of Ichthyology
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    • v.34 no.2
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    • pp.113-118
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    • 2022
  • The feeding habits of sandfish, Arctoscopus japonicus were studied using 781 specimens collected by Eastern Sea Danish and gill net from January 2020 to December 2021 in the coastal waters of East Sea, Korea. The size of the specimens ranged from 14.8 to 25.4 cm in total length. A. japonicus were fed mainly on amphipods that constituted 54.7% in IRI. Cephalopods were the second largest prey component. The diets also include small quantities of euphausiids, crabs, shrimps, fishes, and worms. The diet composition of A. japonicus showed changes in season. The cephalopods feeding rate was highest in summer, whereas the proportion of euphausiids was higher in spring than in other seasons. The proportion of cephalopods has increased as the body size of A. japonicus increased, whereas the proportion of amphipods and euphausiids decreased gradually. As the body size of A. japonicus increased the mean weight of prey per the stomach (mW/ST) tended to increase significantly (One-way ANOVA, P<0.05).

Germination Responses to Mixtures Seeding Rate and Sowing Method of Kentucky Bluegrass and White Clover (캔터키블루그래스와 토끼풀의 파종방법 및 혼파비율에 따른 종자발아 반응)

  • Park, Sun-Yeong;Lee, Sun-Yeong;Yoon, Yong-Han;Ju, Jin-Hee
    • Korean Journal of Organic Agriculture
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    • v.29 no.4
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    • pp.601-612
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    • 2021
  • This study was conducted to evaluate the appropriate sowing method and mixture seeding rate on germination of Kentucky bluegrass (Poa pratensis L.) and white clover (Trifolium repens L.). The experimental design includes two sowing methods and six mixed seeding per each method: BT1:BP0 (broadcast seeding; B, Trifolium repens; T, Poa pratensis; P), BT1:BP2, BT1:BP3, BT3:BT1, BT2:BP1, BT0:BP1 and ST1:SP0 (spot seeding; S), ST1:SP2, ST1:SP3, ST3:ST1, ST2:SP1, ST0:SP1. The germination was the highest for both species when the seeding rate was higher than other species. In overall, the germination of white clover was higher and faster than Kentucky bluegrass. Two plots, BT2:BP1, ST1:ST2, were retained the balanced proportion of the germination rate. Therefore, It was suggested, for maintaining the balanced field, it is better to seed white clover twice Kentucky bluegrass on broadcast seeding and Kentucky bluegrass twice white clover on spot seeding. In regard of sowing method, broadcast seeding is better than spot seeding in terms of increasing the germination. It is necessary to supplement the result for real application by long-term monitoring.