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Hemopoietic Effect of Extracts from Four Parts of Deer Antler on Phenylhydrazine-Induced Hemolytic Anemia in Female Rats (Phenylhydrazine으로 유도한 용혈성 빈혈 흰쥐에 대한 녹용 추출물의 조혈 효과)

  • Lee, Mi-Ra;Kim, Hyun-Ho;Jo, Hyun-Ho;Kang, Hyo-Jin;Gu, LiJuan;Ly, Sun-Young;Lee, Chung-Ha;Kim, Seung-Mi;Yang, Sun-Ah;Mo, Eun-Kyung;Sung, Chang-Keun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.12
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    • pp.1718-1723
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    • 2009
  • This study was to investigate the protection of the extracts from four parts of deer antler in an anemia model induced by intravenous injection of phenylhydrazine·HCl (PHZ) at 10 mg/kg for 4 days. After PHZ injection, female Sprague-Dawley rats were administrated partial deer antler extract (200 mg/kg/day, p.o.) daily for 1 week. Results showed that sever hemolysis was induced by PHZ. For antler extract-treated groups, the concentration of hemoglobin, hematocrit and red blood cells number increased much more significantly than PHZ-treated group. Upper antler extract-treated group was more remarkable than other parts in suppressing the increase of reticulocyte in whole blood. Moreover, antler extract administration significantly improved serum erythropoietin concentration. The activity of $\delta$-aminolevulinic acid dehydrates (ALAD) in liver homogenate was increased in antler extract-treated groups, especially middle and base extract-treated groups showed statistical significance. These results could be concluded that the deer antler extract improved anemia induced by PHZ injection through improving hematological values, serum EPO value, ALAD enzyme activity.

Association of Thymidylate Synthase 5'-UTR 28bp Tandem Repeat and Serine Hydroxymethyltransfarase C1420T Polymorphisms with Susceptibility to Acute Leukemia

  • Dunna, Nageswara Rao;Naushad, Shaik Mohammad;Vuree, Sugunakar;Anuradha, Cingeetham;Sailaja, Kagita;Surekha, Damineni;Rao, Digumarti Raghunadha;Vishnupriya, Satti
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.4
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    • pp.1719-1723
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    • 2014
  • Background: The current study was aimed to elucidate the association of thymidylate synthase (TYMS) 5'-UTR 28bp tandem repeat and cytosolic serine hydroxymethyltransferase (cSHMT) C1420T polymorphisms with acute leukemia in South Indian subjects. A total of 812 subjects [523 healthy controls, 148 acute lymphoblastic leukemia (ALL) cases and 141 acute myeloid leukemia (AML) cases] were screened for TYMS 5'-UTR 28bp tandem repeat and cSHMT C1420T using PCR-AFLP and PCR-with confronting two-pair primers (CTPP) approaches. TYMS 5'-UTR 2R allele frequencies of controls, ALL and AML cases were 35.3%, 28.0% and 30.1% respectively. This polymorphism conferred protection against ALL (OR: 0.71, 95%CI: 0.53-0.96) while showing no statistically significant association with AML (OR: 0.79, 95%CI: 0.58, 1.07). The cSHMT variant allele (T-) frequencies of ALL and AML cases (6.42% and 5.68% respectively) were significantly lower compared to controls (58.3%). This polymorphism conferred protection against ALL (OR: 0.049, 95%CI: 0.029-0.081) and AML (OR: 0.043, 95%CI: 0.025-0.074). The TYMS 5'-UTR 2R2R genotype was associated with a lower total leukocyte count, smaller percentage of blasts, and more adequate platelet count compared to 2R3R and 3R3R genotypes in ALL cases. No such genotype-dependent differences were observed in AML cases. ALL cases carrying the cSHMT C1420T polymorphism showed higher disease free survival compared to those with the wild genotype. To conclude, the TYMS 5'-UTR 28bp tandem repeat reduces risk for ALL while cSHMT C1420T reduces risk for both ALL and AML. Both also influence disease progression in ALL.

Characterization of Plasmid-Mediated SHV-11 β-lactamase Gene of Klebsiella pneumoniae Isolated from the Clinical Specimens (임상검체로부터 분리한 플라스미드 매개성 SHV-11 β-lactamase 유전자의 특성)

  • Kim, Yun-Tae;Lee, Sang-Hoo;Jang, Ji-Hyun;Kim, Tae-Un;Choi, Seok-Cheol;Baik, Hyung-Suk;Lee, Kyoung-Ryul;Yoon, Hye-Ryoung;Kim, Young-Jin
    • Journal of Life Science
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    • v.19 no.12
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    • pp.1718-1723
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    • 2009
  • In this study, we characterized extended-spectrum $\beta$-lactamase (ESBL)-producing Enterobacteriaceae isolated from clinical specimens in Korea and found two strains harboring plasmid-mediated $bla_{SHV-11}$, Klebsiella pneumoniae. First, the isolates were detected using the Vitek system and confirmed by the double-disk synergy test. The classification of gene coding for ESBL was also performed by polymerase chain reactions and followed by DNA sequencing. The transmission of genes was confirmed by transconjugation and transformation. Resistant expression of transformants was determined by broth microdilution minimal inhibitory concentration test. Genotypic analysis revealed that one strain harbored the $bla_{TEM-1}$, $bla_{SHV-11}$ and $bla_{CTX-M-15}$ and the other strain harbored the $bla_{SHV-11}$ and $bla_{CTX-M-15}$. They showed high resistance to oxyiminocephalosphorins (3rd-generation cephalosporins), while the transformant containing only $bla_{SHV-11}$ did not show any resistance to the antibiotics.

Preparation of LiFe PO4 Using Chitosan and its Cathodic Properties for Rechargeable Li-ion Batteries

  • Hong, Kyong-Soo;Yu, Seong-Mi;Ha, Myoung-Gyu;Ahn, Chang-Won;Hong, Tae-Eun;Jin, Jong-Sung;Kim, Hyun-Gyu;Jeong, Euh-Duck;Kim, Yang-Soo;Kim, Hae-Jin;Doh, Chil-Hoon;Yang, Ho-Soon;Jung, Hee
    • Bulletin of the Korean Chemical Society
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    • v.30 no.8
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    • pp.1719-1723
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    • 2009
  • The LiFeP$O_4$ powder was synthesized by using the solid state reaction method with Fe($C_2O_4){\cdot}2H_2O,\;(NH_4)_2HPO_4,\;Li_2CO_3$, and chitosan as a carbon precursor material for a cathode of a lithium-ion battery. The chitosan added LiFePO4 powder was calcined at 350 ${^{\circ}C}$ for 5 hours and then 800 ${^{\circ}C}$ for 12 hours for the calcination. Then we calcined again at 800 ${^{\circ}C}$ for 12 hours. We characterized the synthesized compounds via the crystallinity, the valence states of iron ions, and their shapes using TGA, XRD, SEM, TEM, and XPS. We found that the synthesized powders were carbon-coated using TEM images and the iron ion is substituted from 3+ to 2+ through XPS measurements. We observed voltage characteristics and initial charge-discharge characteristics according to the C rate in LiFeP$O_4$ batteries. The obtained initial specific capacity of the chitosan added LiFeP$O_4$ powder is 110 mAh/g, which is much larger than that of LiFeP$O_4$ only powder.

Disease Resistance against Bacterial Infection on Treatment of Hot-water Extract with 6 Herbal Mixtures in Olive Flounder Paralichthys olivaceus (복합 생약재 열수추출물 투여에 따른 넙치 세균성 질병에 대한 질병저항성)

  • SEO, Jung Soo;JEON, Eun Ji;KWON, Mun Gyeong;HWANG, Jee Youn;JUNG, Sung Hee;KIM, Na Young;JEE, Bo Young;PARK, Myoung Ae
    • Journal of Fisheries and Marine Sciences Education
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    • v.28 no.6
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    • pp.1715-1723
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    • 2016
  • The heat extracts of six kinds of medicinal herbs (Scutellaria baicalensis, Sophora flavescens, Citrus unshiu pericarpium, Lonicera japonica, Perilla frutescens, Benincasa hispida) were tested for non-specific immune response and disease resistance effects related with fish diseases on olive flounder, Paralichthys olivaceus. The preventive effects of 6 herbal mixtures against bacterial disease on cultured flounder were examined follow as feeding EP absorbed with the heat extract of six kinds of medicinal herbs. For feeding trial for 12th week, weight gain and serum analysis of fish fed various groups were not significant differences. Lysozyme activity of the 0.01 % treated group on 4th week showed significant increase. Histopathology of the administrated group for feeding period showed no particular signs of tissue degeneration. At 0.01% oral experimental group, relative percent survival (RPS) were only 50% to 75% for four weeks and eight weeks group by intraperitoneal injection with E. tarda. The results suggest that heat extracts of six kinds of medicinal herbs (0.01%) would be effective to enhance the nonspecific immunity and protective ability of olive flounder against fish pathogen.

Gibberellin A4 Producted by Fusarium solani Isolated from the Roots of Suaeda japonica Makino (칠면초의 뿌리로부터 분리된 Fusarium solani에 의해 생산된 지베렐린 A4)

  • Seo, Yeonggyo;You, Young-Hyun;Yoon, Hyeokjun;Kang, Sang-Mo;Kim, Hyun;Kim, Miae;Kim, Changmu;Lee, In-Jung;Kim, Jong-Guk
    • Journal of Life Science
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    • v.22 no.12
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    • pp.1718-1723
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    • 2012
  • Ten endophytic fungi with different colony morphologies were isolated from the roots of Suaeda japonica Makino growing naturally in Suncheon Bay. Plant growth promotion was verified by treating waito-c rice seedlings with culture filtrates from the endophytic fungi. The bioassays showed that the Sj/7/4 fungal strain induced effective growth promotion in the seedlings. The gibberellins (GA) produced by fungal strain Sj/7/4 were analyzed by gas chromatography /mass spectroscopy with selected ion monitoring (GC/MS SIM). The culture filtrate of Sj/7/4 fungal strain was confirmed to contain $GA_4$ through quantitative analysis. The Sj/7/4 fungal strain was identified to determine the internal transcribed spacer (ITS) regions with universal primers ITS-1 and ITS-4 by using polymerase chain reactions (PCR). Molecular analysis of the Sj/7/4 fungal strain showed high similarity to Fusarium solani. The Sj/7/4 fungal strain isolated from the root of S. japonica was therefore designated as F. solani Sj/7/4.

The type of women in Gongsageunmunrok and A Gongsageunmunrok's meaning of women's history in Joseon (『공사견문록』의 여성유형과 여성생활사 측면에서 본 의의)

  • Kim, Girim
    • (The)Study of the Eastern Classic
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    • no.48
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    • pp.117-145
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    • 2012
  • Gongsageunmunrok is a book that was edited by Jung Jae-ryun. He edited and chose some stories good enough to be an role model to descendants. There were all about 310 stories in that book. A forth of them is story about women. There were 4 types of women in the book. cautious women and women to see through a covered fact.Women who have a lot of authority, women who ask a man something, The women cautious women are generally a royal family. They tried to stabilize their royal family and nation. The way to stabilize was a good behavior. Women who have authority harms equitably and authenticity of official business. Requesting women, also, harm fairness of official business. Gongsageunmunrok has many different meanings like the following. First of all, There are women life of royal family's women in the book. Second, It is the book which can be based on later stories about women. Lee Geng-ik(李肯翊) rewrote on Yeunreusilgisul(燃藜室記述). Especially, Kim Reu rewrote the story titled 'Hansukwonjeon(韓淑媛傳)'. Sung Hae-eung,also, rewote Han Bo-hyang's story. Third, There are many stories with the life of court maid. It is useful to study all the life of court maid, political events and current events. Forth, It describes with women's aspects. Therefore many women's clique can be understood. Fifth, It can show women's literary work and a phase of women's literary activity.

Improvisation and Fantasy: Beauvais Grotesque Tapestry (즉흥과 환상: 보베 제작소의 《그로테스크》 태피스트리)

  • Chung, Eunjin
    • Art History and Visual Culture
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    • no.21
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    • pp.126-147
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    • 2018
  • This paper is to find the theme and meaning of the Beauvais Grotesque tapestry considered a conundrum. The three works are chosen from Beauvais Grotesque consisting of six tapestries, which are The Offering to Pan, The Musician and Dancers, The Camel in the J. Paul Getty Museum. I analyzed these works by dividing them into grotesque ornaments, chinoiserie motifs, and scenography. The Offering to Pan shows the influence of Raphael's Grotesque tapestry, but Beauvais work followed the design of Jean Berain's Grotesque with arabesque. In The Musician and Dancers, chinese ceramics, textiles, and Chinese people in edge were noted. Especially, the Chinese with yellow skin in the border reveals the European gaze on China at that time. In the 18th century, Chinoiserie was prevalent through stage designs rather than books. The Camel, playing Brighella, makes it clear that this tapestry is a stage of Comedia dell'arte. The characteristic of Comedia is a mixture of genres such as music and dance, with no scripting 'improvisation.' Features of Comedia are 'improvisation' without a script and a mixture of genres such as music and dance. Thus, the Grotesque tapestry transfers the stage of the Comedia into threads woven. In addition, the horizontal stage decoration with the disappearance of the perspective vanishing point is related to the era of regent of Philippe d'Orl?ans (1674-1723). Above all, the grotesque, Chinoiserie, and scenography are all fantasies separated from reality. Therefore, the Beauvais Grotesque tapestry represents of 'improvisation' and 'fantasy' in which there is no narrative theme or meaning, as if the script of Comedia did not exist.

Landslide Susceptibility Mapping Using Deep Neural Network and Convolutional Neural Network (Deep Neural Network와 Convolutional Neural Network 모델을 이용한 산사태 취약성 매핑)

  • Gong, Sung-Hyun;Baek, Won-Kyung;Jung, Hyung-Sup
    • Korean Journal of Remote Sensing
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    • v.38 no.6_2
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    • pp.1723-1735
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    • 2022
  • Landslides are one of the most prevalent natural disasters, threating both humans and property. Also landslides can cause damage at the national level, so effective prediction and prevention are essential. Research to produce a landslide susceptibility map with high accuracy is steadily being conducted, and various models have been applied to landslide susceptibility analysis. Pixel-based machine learning models such as frequency ratio models, logistic regression models, ensembles models, and Artificial Neural Networks have been mainly applied. Recent studies have shown that the kernel-based convolutional neural network (CNN) technique is effective and that the spatial characteristics of input data have a significant effect on the accuracy of landslide susceptibility mapping. For this reason, the purpose of this study is to analyze landslide vulnerability using a pixel-based deep neural network model and a patch-based convolutional neural network model. The research area was set up in Gangwon-do, including Inje, Gangneung, and Pyeongchang, where landslides occurred frequently and damaged. Landslide-related factors include slope, curvature, stream power index (SPI), topographic wetness index (TWI), topographic position index (TPI), timber diameter, timber age, lithology, land use, soil depth, soil parent material, lineament density, fault density, normalized difference vegetation index (NDVI) and normalized difference water index (NDWI) were used. Landslide-related factors were built into a spatial database through data preprocessing, and landslide susceptibility map was predicted using deep neural network (DNN) and CNN models. The model and landslide susceptibility map were verified through average precision (AP) and root mean square errors (RMSE), and as a result of the verification, the patch-based CNN model showed 3.4% improved performance compared to the pixel-based DNN model. The results of this study can be used to predict landslides and are expected to serve as a scientific basis for establishing land use policies and landslide management policies.