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http://dx.doi.org/10.5352/JLS.2009.19.12.1718

Characterization of Plasmid-Mediated SHV-11 β-lactamase Gene of Klebsiella pneumoniae Isolated from the Clinical Specimens  

Kim, Yun-Tae (Department of Microbiology Genetics, Seoul Medical Science Institute/Seoul Clinical Laboratories)
Lee, Sang-Hoo (Department of Microbiology Genetics, Seoul Medical Science Institute/Seoul Clinical Laboratories)
Jang, Ji-Hyun (Department of Microbiology Genetics, Seoul Medical Science Institute/Seoul Clinical Laboratories)
Kim, Tae-Un (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan)
Choi, Seok-Cheol (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan)
Baik, Hyung-Suk (Division of Biological Science, Pusan National University)
Lee, Kyoung-Ryul (Department of Microbiology Genetics, Seoul Medical Science Institute/Seoul Clinical Laboratories)
Yoon, Hye-Ryoung (Department of Microbiology Genetics, Seoul Medical Science Institute/Seoul Clinical Laboratories)
Kim, Young-Jin (Department of Microbiology Genetics, Seoul Medical Science Institute/Seoul Clinical Laboratories)
Publication Information
Journal of Life Science / v.19, no.12, 2009 , pp. 1718-1723 More about this Journal
Abstract
In this study, we characterized extended-spectrum $\beta$-lactamase (ESBL)-producing Enterobacteriaceae isolated from clinical specimens in Korea and found two strains harboring plasmid-mediated $bla_{SHV-11}$, Klebsiella pneumoniae. First, the isolates were detected using the Vitek system and confirmed by the double-disk synergy test. The classification of gene coding for ESBL was also performed by polymerase chain reactions and followed by DNA sequencing. The transmission of genes was confirmed by transconjugation and transformation. Resistant expression of transformants was determined by broth microdilution minimal inhibitory concentration test. Genotypic analysis revealed that one strain harbored the $bla_{TEM-1}$, $bla_{SHV-11}$ and $bla_{CTX-M-15}$ and the other strain harbored the $bla_{SHV-11}$ and $bla_{CTX-M-15}$. They showed high resistance to oxyiminocephalosphorins (3rd-generation cephalosporins), while the transformant containing only $bla_{SHV-11}$ did not show any resistance to the antibiotics.
Keywords
Extended-spectrum ${\beta}$-lactamase; $bla_{SHV-11}$; $bla_{CTX-M-15}$; oxyiminocephalosphorins; Klebsiella pneumoniae;
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