• 제목/요약/키워드: (prime)radical

검색결과 96건 처리시간 0.022초

추출 방법이 개두릅 추출물의 항산화 활성에 미치는 영향 (Antioxidative Activity of Kalopanax pictus Shoot Extracted Using Different Extraction Methods)

  • 강경명;노홍균;박창수;윤광섭;홍주헌;이신호
    • 한국식품영양과학회지
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    • 제41권12호
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    • pp.1686-1692
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    • 2012
  • 개두릅 추출물의 항산화 활성을 증가시킬 수 있는 적정추출방법을 개발하기 위하여 상온교반, 초음파, 감압, 환류냉각, 환류냉각과 초음파 병행 추출법을 이용하여 추출한개두룹 추출물의 항산화 활성을 비교하였다. 추출수율은 환류냉각과 초음파 병행 추출물에서 높게 나타났다. 총 폴리페놀 및 총 플라보노이드의 함량 또한 환류냉각과 초음파 병행추출물이 각각 153.43 mg GAE/g, 62.53 mg RE/g으로 높았으며, 모든 추출물은 항산화 실험에서 농도 증가에 비례하여 활성이 증가하였다. 환류냉각과 초음파 병행 추출물은 추출농도 1,000 ${\mu}g/mL$에서 DPPH와 ABTS 라디칼 소거능(90.85%, 99.83%), 환원력(OD 0.95, 700 nm), 아질산염 소거능(55.46%), ferrous ion chelating 효과(45.12%)가 우수하거나 유사하였다.

배를 이용한 발효식초의 품질특성 (Quality characteristics of fermented vinegars using pear)

  • 박연옥
    • 한국식품저장유통학회지
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    • 제23권6호
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    • pp.778-787
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    • 2016
  • 본 연구는 배를 주소재로 이용하고 부재료로 흑미와 애플민트를 이용하여 발효식초를 제조하여 각 식초의 이화학적인 품질특성 및 항산화 활성을 비교하여 기능성 발효식초의 품질향상을 위한 기초자료를 제공하고자 실시하였다. 각 식초의 pH, 총산도, 당도, 색도, 아미노산 함량, 관능평가, 총 폴리페놀 및 플라보노이드 함량, DPPH 라디칼 소거능을 분석하였다. 대조군으로 곡류초인 현미식초를 이용하였다. 배를 이용한 발효식초는 pH 3.17~3.43 범위 내에서 유의적인 차이(p<0.05)를 보였고, 총산도는 4.01~5.05%으로 배흑미식초가 가장 높았다. 당 함량은 식초 제조 시 첨가된 설탕과 배의 당도가 합쳐져 유의적으로 배식초와 배민트식초가 높았다(p<0.05). 식초의 색도는 배식초의 명도가 유의적으로 높았으며 적색도는 배흑미식초, 황색도는 배민트식초가 높아 부수적으로 첨가한 재료에 따라 유의적인 차이를 보였다(p<0.05). 필수아미노산은 배식초가 유의적으로 lysine, isoleucine, valine, threonine 순으로 많았고 다음으로 현미식초가 많이 함유하였다 (p<0.05). 비필수아미노산은 전체적으로 glutamic acid와 aspartic acid의 함량이 많았으며 glutamic acid는 현미식초, 배흑미식초, 배식초, 배민트식초 순이었고 aspartic acid는 배식초, 배민트식초, 현미식초, 배흑미식초 순이었다(p<0.05). 총 폴리페놀 함량은 유의적으로 배식초가 가장 많았으며 배흑미식초나 현미식초는 유사하고 배민트식초가 가장 적었다(p<0.05). 총 플라보노이드 함량은 배흑미식초가 가장 많았고 배민트식초, 배식초 순을 보였으며 현미식초는 현저하게 낮았다. DPPH 라디칼소거능은 배식초의 소거활성이 유의적으로 가장 높았으며(p<0.05) 배흑미식초, 현미식초, 배민트식초 순으로 나타나 식초의 항산화활성에 주로 기여하는 물질은 폴리페놀 화합물임을 비례적으로 나타내었다. 식초의 관능평가 결과 강도 뿐 아니라 기호도에서도 배흑미식초가 유의적으로 가장 높게 나왔다(p<0.05). 결론적으로 배를 이용한 발효식초는 배식초의 품질특성이 가장 우수하였고 전체적으로 저산도식초로서 아미노산 함량이나 총 폴리페놀 및 플라보노이드 함량이 높고 DPPH 라디칼 소거능이 우수하여 조미용 뿐 아니라 음료용 식초로도 폭넓게 이용하면 국민건강 향상을 위한 기능성 발효식초 음료로 활용도가 높을 것으로 사료된다.

산지별 홍국발효 참당귀의 이화학적 특성 및 생리활성 (Physicochemical Characteristics and Biological Activities of Monascus-fermented Angelica gigas Nakai by Origin)

  • 박규림;김영완;김태훈;안희영;심소연;김정욱;조영수
    • 생명과학회지
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    • 제27권8호
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    • pp.919-929
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    • 2017
  • 본 연구는 발효 참당귀를 이용한 건강 기능성 식품개발을 위해 산청, 평창 및 제천산 참당귀를 Monascus purpureus로 발효시켜 제조한 발효 참당귀의 이화학적 특성 및 생리활성을 평가하였다. 발효 참당귀의 decursin 및 decursinol angelate 함량은 발효전보다 증가한 바, 평창 참당귀(PA)군의 615.504 및 326.742 ppm이 발효 후 각각 792.610 및 429.500 ppm으로 증가되었다. 총 페놀성 화합물과 플라보노이드 및 미네랄 함량이 발효로 인해 증가하였고, DPPH를 이용한 자유라디칼 소거능 및 Fe, Cu를 이용한 환원력 비교를 통해 발효 참당귀군에서 높게 나타났으며, 이중 FPA군에서 활성이 높은 것을 확인하였다. LPS처리 RAW 264.7세포의 NO생성능과 유방암 세포(MCF-7) 및 간암 세포(Hep3B) 성장 저해능 확인 결과 발효 참당귀군에서 활성이 높게 나타났다. 이와 같이 M. purpureus 발효 산청, 평창 및 제천 참당귀를 비교한 결과 평창 참당귀에서 건강 기능성 식품으로의 활용 가치가 높을 것으로 사료된다.

발효 동충하초의 이화학적 특성 (Chemical Characteristics in Fermented Cordycepin-enriched Cordyceps militaris)

  • 안희영;박규림;김유라;차재영;조영수
    • 생명과학회지
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    • 제23권8호
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    • pp.1032-1040
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    • 2013
  • 유용발효미생물에 의한 cordycepin 고함유 동충하초 분말의 생리활성 작용 및 물질을 개발하기 위한 기초 자료를 얻을 목적으로 2 종의 유산균 Lactobacillus hilgardii (Lh), Lactobacillus acidophilus (La)와 1 종의 효모 Saccharomyces cerevisiae (Sc), 1 종의 바실러스 Bacillus subtilis (Bs), 4 종의 곰팡이 Aspergillus oryzae (Ao), Aspergillus kawachii (Ak) 및 Monascus purpureus (Mp), Rhizopus oryzae (Ro)를 본 실험에 사용하였으며, 이들에 의한 발효 cordycepin 고함유 동충하초 분말의 미네랄 함량, 지방산 조성, 단백질 함량, 단백질 패턴, 항산화 활성, 혈전용해 활성을 비교 검토하였다. 발효 cordycepin 고함유 동충하초분말의 주요 미네랄은 미네랄 성분 조성은 K, Mg, Ca, Na, Fe, Zn로 나타났으며, 전체적으로 K, Mg, Ca 순으로 높은 비율을 차지하였고, 주요 지방산 성분으로 palmitic acid, stearic acid, oleic acid, linoleic acid, linolenic acid 이다. 동충하초 분말과 cordycepin 고함유 동충하초분말 및 발효시킨 cordycepin 고함유 동충하초 분말 단백질을 native-PAGE로 비교분석 한 결과 발효에 의해 균주에 상관없이 특정 부분의 단백질 분해를 확인하였고, SDS-PAGE 상의 단백질 패턴 분석에서는 66 kDa 크기의 단백질 밴드가 유산균 발효에 의해 분해 되어 거의 관찰되지 않았으며, Aspergillus oryzae에서 새로운 단백질 밴드를 확인하였다. Cordycepin 고함유 동충하초 분말을 유용미생물로 발효시킨 결과 Bacillus subtilis 균주 발효에서 혈전용해 활성이 17.1 unit으로 높게 나왔으며, Lactobacillus hilgardii 균주 발효에 의해서는 7.2 unit으로 낮은 혈전용해 활성이 나왔다. 항산화 활성은 발효시킨 cordycepin 고함유 동충하초 분말에서 균주 종류에 관계없이 거의 비슷하게 나왔지만 시판 항산화제 BHT 처리구의 90% 이상의 높은 항산화 활성보다는 낮았다. 이상의 실험결과에서 유용미생물 발효에 의한 cordycepin 고함유 동충하초 분말의 이화학적 특성이 발효 전 cordycepin 고함유 동충하초 분말보다 강화됨으로써 건강식품의 소재로서의 이용 가능성이 높다고 할 수 있다.

유용 효모균주를 이용한 발효참당귀분말 추출물의 이화학적 특성 및 생리활성 효과 (Physicochemical Properties and Biological Activities of Angelica gigas Fermented by Saccharomyces cerevisiae)

  • 심소연;박우상;신현승;옥민;조영수;안희영
    • 생명과학회지
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    • 제29권10호
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    • pp.1136-1143
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    • 2019
  • 본 연구는 Saccharomyces cerevisiae를 이용하여 발효한 참당귀 분말을 수용성 용매에 추출하여 이화학적 특성 및 생리활성을 비교 검토하였다. 당도 및 pH를 확인한 결과, NF군 $1^{\circ}Brix$에 비해 F/3군 $0.9^{\circ}Brix$로 발효가 됨에 따라 당도가 감소하는 경향을 보였다. 한편, F/3군의 pH는 NF군 및 F/0군에 비해서 높은 수치를 나타냈다. Thin-layer chromatography 실험결과, F/3군이 NF군에 비해 대부분 저분자의 당 형태로 분해된 것을 확인하였다. 총 폴리페놀 함량 및 플라보노이드 함량은 참당귀 분말 추출물에 비해 발효 참당귀 분말 추출물에서 전반적으로 함량이 증가하였다. 미네랄 중 K 및 Ca 함량은 F/3군, F/0군 및 NF군 순으로 증가하는 경향을 보였다. Decursin 및 decursinol angelate 함량은 NF군보다 F/3군에서 더 높은 함량을 나타냈다. 참당귀 및 발효 참당귀 수용성 추출물의 항산화 활성은 NF군 41.89%, F/0군 39.51%, F/3군에서 60.26%으로 활성이 증가하는 것을 확인하였다. 미백 및 항염증 효능 실험결과, 참당귀 분말 추출물에 비해 발효 참당귀 분말 추출물에서 효능이 증가하는 경향을 나타내었다. 따라서 한방 생약재 중 참당귀를 유용 미생물로 발효시켜 사용함으로써 항산화능, 생리활성물질, 미백효능 및 항염증 효과에서 긍정적인 결과를 나타내어 향후 화장품 개발을 위한 소재로써 기대할 수 있을 것으로 사료된다.

한국전쟁의 교훈과 대비 -병력수(兵力數) 및 부대수(部隊數)를 중심으로- (The lesson From Korean War)

  • 윤일영
    • 안보군사학연구
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    • 통권8호
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    • pp.49-168
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    • 2010
  • Just before the Korean War, the total number of the North Korean troops was 198,380, while that of the ROK(Republic of Korea) army troops 105,752. That is, the total number of the ROK army troops at that time was 53.3% of the total number of the North Korean army. As of December 2008, the total number of the North Korean troops is estimated to be 1,190,000, while that of the ROK troops is 655,000, so the ROK army maintains 55.04% of the total number of the North Korean troops. If the ROK army continues to reduce its troops according to [Military Reform Plan 2020], the total number of its troops will be 517,000 m 2020. If North Korea maintains the current status(l,190,000 troops), the number of the ROK troops will be 43.4% of the North Korean army. In terms of units, just before the Korean War, the number of the ROK army divisions and regiments was 80% and 44.8% of North Korean army. As of December 2008, North Korea maintains 86 divisions and 69 regiments. Compared to the North Korean army, the ROK army maintains 46 Divisions (53.4% of North Korean army) and 15 regiments (21.3% of North Korean army). If the ROK army continue to reduce the military units according to [Military Reform Plan 2020], the number of ROK army divisions will be 28(13 Active Division, 4 Mobilization Divisions and 11 Local Reserve Divisions), while that of the North Korean army will be 86 in 2020. In that case, the number of divisions of the ROK army will be 32.5% of North Korean army. During the Korean war, North Korea suddenly invaded the Republic of Korea and occupied its capital 3 days after the war began. At that time, the ROK army maintained 80% of army divisions, compared to the North Korean army. The lesson to be learned from this is that, if the ROK army is forced to disperse its divisions because of the simultaneous invasion of North Korea and attack of guerrillas in home front areas, the Republic of Korea can be in a serious military danger, even though it maintains 80% of military divisions of North Korea. If the ROK army promotes the plans in [Military Reform Plan 2020], the number of military units of the ROK army will be 32.5% of that of the North Korean army. This ratio is 2.4 times lower than that of the time when the Korean war began, and in this case, 90% of total military power should be placed in the DMZ area. If 90% of military power is placed in the DMZ area, few troops will be left for the defense of home front. In addition, if the ROK army continues to reduce the troops, it can allow North Korea to have asymmetrical superiority in military force and it will eventually exert negative influence on the stability and peace of the Korean peninsular. On the other hand, it should be reminded that, during the Korean War, the Republic of Korea was attacked by North Korea, though it kept 53.3% of troops, compared to North Korea. It should also be reminded that, as of 2008, the ROK army is defending its territory with the troops 55.04% of North Korea. Moreover, the national defense is assisted by 25,120 troops of the US Forces in Korea. In case the total number of the ROK troops falls below 43.4% of the North Korean army, it may cause social unrest about the national security and may lead North Korea's misjudgement. Besides, according to Lanchester strategy, the party with weaker military power (60% compared to the party with stronger military power) has the 4.1% of winning possibility. Therefore, if we consider the fact that the total number of the ROK army troops is 55.04% of that of the North Korean army, the winning possibility of the ROK army is not higher than 4.1%. If the total number of ROK troops is reduced to 43.4% of that of North Korea, the winning possibility will be lower and the military operations will be in critically difficult situation. [Military Reform Plan 2020] rums at the reduction of troops and units of the ground forces under the policy of 'select few'. However, the problem is that the financial support to achieve this goal is not secured. Therefore, the promotion of [Military Reform Plan 2020] may cause the weakening of military defence power in 2020. Some advanced countries such as Japan, UK, Germany, and France have promoted the policy of 'select few'. However, what is to be noted is that the national security situation of those countries is much different from that of Korea. With the collapse of the Soviet Unions and European communist countries, the military threat of those European advanced countries has almost disappeared. In addition, the threats those advanced countries are facing are not wars in national level, but terrorism in international level. To cope with the threats like terrorism, large scaled army trops would not be necessary. So those advanced European countries can promote the policy of 'select few'. In line with this, those European countries put their focuses on the development of military sections that deal with non-military operations and protection from unspecified enemies. That is, those countries are promoting the policy of 'select few', because they found that the policy is suitable for their national security environment. Moreover, since they are pursuing common interest under the European Union(EU) and they can form an allied force under NATO, it is natural that they are pursing the 'select few' policy. At present, NATO maintains the larger number of troops(2,446,000) than Russia(l,027,000) to prepare for the potential threat of Russia. The situation of japan is also much different from that of Korea. As a country composed of islands, its prime military focus is put on the maritime defense. Accordingly, the development of ground force is given secondary focus. The japanese government promotes the policy to develop technology-concentrated small size navy and air-forces, instead of maintaining large-scaled ground force. In addition, because of the 'Peace Constitution' that was enacted just after the end of World War II, japan cannot maintain troops more than 240,000. With the limited number of troops (240,000), japan has no choice but to promote the policy of 'select few'. However, the situation of Korea is much different from the situations of those countries. The Republic of Korea is facing the threat of the North Korean Army that aims at keeping a large-scale military force. In addition, the countries surrounding Korea are also super powers containing strong military forces. Therefore, to cope with the actual threat of present and unspecified threat of future, the importance of maintaining a carefully calculated large-scale military force cannot be denied. Furthermore, when considering the fact that Korea is in a peninsular, the Republic of Korea must take it into consideration the tradition of continental countries' to maintain large-scale military powers. Since the Korean War, the ROK army has developed the technology-force combined military system, maintaining proper number of troops and units and pursuing 'select few' policy at the same time. This has been promoted with the consideration of military situation in the Koran peninsular and the cooperation of ROK-US combined forces. This kind of unique military system that cannot be found in other countries can be said to be an insightful one for the preparation for the actual threat of North Korea and the conflicts between continental countries and maritime countries. In addition, this kind of technology-force combined military system has enabled us to keep peace in Korea. Therefore, it would be desirable to maintain this technology-force combined military system until the reunification of the Korean peninsular. Furthermore, it is to be pointed out that blindly following the 'select few' policy of advanced countries is not a good option, because it is ignoring the military strategic situation of the Korean peninsular. If the Republic of Korea pursues the reduction of troops and units radically without consideration of the threat of North Korea and surrounding countries, it could be a significant strategic mistake. In addition, the ROK army should keep an eye on the fact the European advanced countries and Japan that are not facing direct military threats are spending more defense expenditures than Korea. If the ROK army reduces military power without proper alternatives, it would exert a negative effect on the stable economic development of Korea and peaceful reunification of the Korean peninsular. Therefore, the desirable option would be to focus on the development of quality of forces, maintaining proper size and number of troops and units under the technology-force combined military system. The tableau above shows that the advanced countries like the UK, Germany, Italy, and Austria spend more defense expenditure per person than the Republic of Korea, although they do not face actual military threats, and that they keep achieving better economic progress than the countries that spend less defense expenditure. Therefore, it would be necessary to adopt the merits of the defense systems of those advanced countries. As we have examined, it would be desirable to maintain the current size and number of troops and units, to promote 'select few' policy with increased defense expenditure, and to strengthen the technology-force combined military system. On the basis of firm national security, the Republic of Korea can develop efficient policies for reunification and prosperity, and jump into the status of advanced countries. Therefore, the plans to reduce troops and units in [Military Reform Plan 2020] should be reexamined. If it is difficult for the ROK army to maintain its size of 655,000 troops because of low birth rate, the plans to establish the prompt mobilization force or to adopt drafting system should be considered for the maintenance of proper number of troops and units. From now on, the Republic of Korean government should develop plans to keep peace as well as to prepare unexpected changes in the Korean peninsular. For the achievement of these missions, some options can be considered. The first one is to maintain the same size of military troops and units as North Korea. The second one is to maintain the same level of military power as North Korea in terms of military force index. The third one is to maintain the same level of military power as North Korea, with the combination of the prompt mobilization force and the troops in active service under the system of technology-force combined military system. At present, it would be not possible for the ROK army to maintain such a large-size military force as North Korea (1,190,000 troops and 86 units). So it would be rational to maintain almost the same level of military force as North Korea with the combination of the troops on the active list and the prompt mobilization forces. In other words, with the combination of the troops in active service (60%) and the prompt mobilization force (40%), the ROK army should develop the strategies to harmonize technology and forces. The Korean government should also be prepared for the strategic flexibility of USFK, the possibility of American policy change about the location of foreign army, radical unexpected changes in North Korea, the emergence of potential threat, surrounding countries' demand for Korean force for the maintenance of regional stability, and demand for international cooperation against terrorism. For this, it is necessary to develop new approaches toward the proper number and size of troops and units. For instance, to prepare for radical unexpected political or military changes in North Korea, the Republic of Korea should have plans to protect a large number of refugees, to control arms and people, to maintain social security, and to keep orders in North Korea. From the experiences of other countries, it is estimated that 115,000 to 230,000 troops, plus ten thousands of police are required to stabilize the North Korean society, in the case radical unexpected military or political change happens in North Korea. In addition, if the Republic of Korea should perform the release of hostages, control of mass destruction weapons, and suppress the internal wars in North Korea, it should send 460,000 troops to North Korea. Moreover, if the Republic of Korea wants to stop the attack of North Korea and flow of refugees in DMZ area, at least 600,000 troops would be required. In sum, even if the ROK army maintains 600,000 troops, it may need additional 460,000 troops to prepare for unexpected radical changes in North Korea. For this, it is necessary to establish the prompt mobilization force whose size and number are almost the same as the troops in active service. In case the ROK army keeps 650,000 troops, the proper number of the prompt mobilization force would be 460,000 to 500,000.

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