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http://dx.doi.org/10.5352/JLS.2013.23.8.1032

Chemical Characteristics in Fermented Cordycepin-enriched Cordyceps militaris  

Ahn, Hee-Young (Department of Medical Biosciences, Graduate School, Dong-A University)
Park, Kyu-Rim (Department of Medical Biosciences, Graduate School, Dong-A University)
Kim, Yu-Ra (Department of Biotechnology, Dong-A University)
Cha, Jae-Young (Technical Research Institute, Daesun Distilling Co., Ltd.)
Cho, Young-Su (Department of Biotechnology, Dong-A University)
Publication Information
Journal of Life Science / v.23, no.8, 2013 , pp. 1032-1040 More about this Journal
Abstract
The comparative effects of fibrinolytic, antioxidative activity and electrophoretical protein patterns with Cordyceps militaris powder (CM) and cordycepin-enriched Cordyceps militaris JLM0636 powder (CCM) and fermented cordycepin-enriched Cordyceps militaris JLM0636 powder by several microscopic organisms were investigated. In addition, nutritional materials such as mineral, protein, and fatty acids were also measured. The protein concentration was higher in CCM than that in CM. The protein concentration in fermented CCM was the highest in CCM treated by Aspergillus kawachii among the various samples. When total protein patterns of CM, CCM, and both fermented CCMs were analyzed by native- and SDS-PAGE, there were slightly varietal differences in electrophoretical protein patterns. Major minerals were K, Ca, Mg, and Zn. Major fatty acids were palmitic acid, stearic acid, oleic acid, linoleic acid, and linolenic acid. Fibrinolytic activity was the highest in the fermented CCM by Bs treatment among the various samples. The ${\alpha},{\alpha}^{\prime}$-diphenyl-${\beta}$-picrylhydrazyl (DPPH) radical scavenging activity was slightly stronger in the CCM treated with Aspergillus kawachii among the various samples; however, these samples all exhibited relatively low levels of activity compared with the butylated hydroxytoluene (BHT). These results may provide the basic data necessary to understand the biological activities and chemical characteristics of Cordyceps militaris JLM0636 powder fermented by several microscopic organisms to develop functional foods.
Keywords
Cordyceps militaris JLM0636; fermentation; fibrinolytic activity; antioxidative activity; cordycepin;
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1 Cha, J. Y., Kim, H. J., Chung, C. H. and Cho, Y. S. 1999. Antioxidative activities and contents of polyphenolic compound of Cudrania tricuspidata. J Korean Soc Food Sci Nutr 28, 1310-1315.   과학기술학회마을
2 Cha, J. Y., Yang, H. J., Jeong, J. J., Seo, W. S., Park, J. S., Ok, M. and Cho, Y. S. 2010. Tyrosinase inhibition activity and antioxidant capacity by fermented products of some medicinal plants. J Life Sci 20, 940-947.   과학기술학회마을   DOI   ScienceOn
3 Cha, J. Y., Park, J. C., Ahn, H. Y., Eom, K. E., Park, B. K., Jun, B. S. and Cho, Y. S. 2009. Effect of Monascus purpureus fermented Korean red ginseng powder on the serum lipid levels and antioxidative activity in rats. J Korean Soc Food Sci Nutr 38, 1153-1160.   과학기술학회마을   DOI   ScienceOn
4 Cha, J. Y., Cho, Y. S., Kim, I., Anno, T., Rahman, S. M. and Yanagita, T. 2001. Effect of hesperetin, a citrus flavonoid, on the liver triacylglycerol content and phosphatidate phosphohydrolase activity in orotic acid-fed rats. Plant Foods Human Nutr 56, 349-358.   DOI   ScienceOn
5 Cha, J. Y., Kim, Y. S., Ahn, H. Y., Eom, K. E., Park, B. K. and Cho, Y. S. 2009. Biological activity of fermented silkworm powder. J Life Sci 19, 1468-1477.   과학기술학회마을   DOI   ScienceOn
6 Cho, H. J., Cho, J. Y., Rhee, M. H., Kim, H. S., Lee, H. S. and Park, H. J. 2007. Inhibitory effects of cordycepin (3'-deoxyadenosine), a component of Cordyceps militaris, on human platelet aggregation induced by thapsigargin. J Microbiol Biotechnol 17, 1134-1138.   과학기술학회마을
7 Choi, H. J., Lee, W. S., Hwang, S. J., Lee, I. J., Shin, D. H., Kim, H. Y. and Kim, K. U. 2000. Changes in chemical compositions of green tea under the different extraction conditions. Korean J Life Sci 10, 202-209.   과학기술학회마을
8 Choi, Y. M., Gu, J. B., Kim, M. H. and Lee, J. S. 2008. Antioxidant and antiproliferative activities of methanolic extracts from thirty Korean medicinal plants. Food Sci Biotechnol 17, 1235-1239.   과학기술학회마을
9 Davis, B. J. 1964. Disc electrophoresis-II. Method and application to human serum proteins. Ann N Y Acad Sci 28, 404-427.
10 Duncan, D. B. 1959. Multiple range and multiple F test. Biometrics 1, 1-42.
11 Fuller, R. 1991. Probiotics in human medicine. Gut 32, 439-442.   DOI   ScienceOn
12 Garces, R. and Mancha, M. 1993. One-step lipid extraction and fatty acid methyl esters preparation from fresh plant tissues. Anal Biochem 211, 139-143.   DOI   ScienceOn
13 Jia, Z., Tang, M. and Wu, J. 1999. The determination of flavonoid contents in mulberry and thier scavenging effects on superoxide radicals. Food Chem 64, 555-559.   DOI   ScienceOn
14 Kang, P. D., Yoon, H. J., Ryu, K. S., Sohn, B. H. and Sohn, H. D. 1999. Electrophoretic patterns of hemolymph proteins of varieties with long and short life span in the silkworm Bombyx mori L. Korean J Seric Sci 41, 1-8.   과학기술학회마을
15 Kim, D. H., Han, S. B., Park, J. S., Han, M. J. 1994. Fermentation of antler and its biological activity. Korean J Pharmacogn 25, 233-237.   과학기술학회마을
16 Kim, I. J., Kim, H. K., Jong, J. H., Jeong, Y. K. and Ryu, C. H. 2002. Study of functional Chungkukang contain fibrinolytic enzyme. Korean J Life Sci 12, 357-362.   DOI   ScienceOn
17 Kwon, H. J., Lee, K. H., Kim, J. H., Chun, S. S., Cho, Y. J. and Cha, W. S. 2006. Effect of protease on the extraction and properties of the protein from silkworm pupa. J Korean Soc Appl Biol Chem 49, 304-308.   과학기술학회마을
18 Luthringer, C., Rayssiguier, Y., Gueux, E. and Berthelot, A. 1988. Effect of moderate magnesium deficiency on serum lipids, blood pressure and cardiovascular reactivity in normotensive rats. Br J Nutr 59, 243-250.   DOI   ScienceOn
19 Li, S. P., Zhang, G. H., Zeng, Q., Huang, Z. G., Wang, Y. T., Dong, T. T. and Tsim, K. W. 2006. Hypoglycemic activity of polysaccharide, with antioxidation, isolated from cultured Cordyceps mycelia. Phytomedicine 13, 428-433.   DOI   ScienceOn
20 Lowry, O. H., Rosebrogh, N. J., Farr, A. L. and Randall, R. J. 1951. Protein measurement with the Folin phenol reagent. J Biol Chem 193, 265-271.
21 Muller, W. E., Weiler, B. E., Charubala, R., Pfleiderer, W., Leserman, L., Sobol, R. W., Suhadolnik, R. J. and Schroder, H. C. 1991. Cordycepin analogues of 2', 5'-oligo-adenylate inhibition of reverse transcriptase. Biochemistry 30, 2027-2033.   DOI   ScienceOn
22 Owens, C. W. I. and Belcher, R. V. 1965. A colorimetric micro- method for the determination of glutathione. Biochem J 94, 705-711.
23 Sung, J. M., Choi, Y. S., Lee, H. K., Kim, S. H., Kim, Y. O. and Sung, G. H. 1999. Production of fruitingbody using cultures of Entomopathogenic fungal species. Korean J Mycol 27, 15-19.
24 Swain, T., Hillis, W. E. and Oritega, M. 1959. Phenolic constituents of Ptunus domestica. I. Quantitative analysis of phenolic constituents. J Sci Food Agric 10, 83-88.
25 Tsukahara, M., Shinzato, N., Tamaki, Y., Namihira, T. and Matsui, T. 2009. Red yeast rice fermentation by selected Monascus sp. with deep-red color, lovastatin production but no citrinin, and effect of temperature-shift cultivation on lovastatin production. Appl Biochem Biotechnol 158, 476-482.   DOI   ScienceOn
26 Weber, K. and Osborn, M. 1969. The reliability of molecular weight determination by sodium dodesyl sulphate-polyacrylamide gel electrophoresis. J Biol Chem 244, 4406-4412.
27 A.O.A.C. 1975. Official methods of analysis. 12th ed., Association of official analytical chemists. Washington, DC, USA.
28 Sugimura, Y. and Yamamoto, K. 1998. The protective effect of glutathione-enriched yeast extract on acetaminophen-induced liver damage in rats. J Jpn Soc Nutr Food Sci 51, 189-193.   DOI
29 Yun, J. S. 2009. Pathomorphological peculiarity of dometic silkworm, Bombyx mori. infected with Cordyceps, Paecilomyces tenuipes. Korean J Pesticide Sci 13, 177-184.   과학기술학회마을
30 Zhu, J. S., Halpern, G. M. and Jones, K. 1998. The scientific rediscovery of an ancient Chinese herbal medicine, Cordyceps sinensis Part I. J Altern Complement Med 4, 289-303.   DOI   ScienceOn
31 Astrup, T. and Mullertz, S. 1991. The fibrin plate method for estimating fibrinolytic activity. Arch Biochem Biophys 40, 346-351.
32 Bate-Smith, E. C. 1954. Flavonoid compounds in foods. Adv food Res 5, 216.
33 Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 26, 1199-1204.
34 Bok, J. W., Ller, J. C., Klin, H. G. and Neil, G. H. 1999. Antitumor sterols from the mycelia of Cordyceps sinensis. Phytochemistry 51, 891-898.   DOI   ScienceOn
35 Cabras, P. and Angioni, A. 2000. Pesticide residues in grapes, wine, and their processing products. J Agric Food Chem 48, 967-973.   DOI   ScienceOn