• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2010.05a
• /
• pp.1403-1404
• /
• 2010

• The Korean Journal of Physiology and Pharmacology
• /
• v.3 no.6
• /
• pp.579-586
• /
• 1999

Complement-mediated neutrophil activation has been hypothesized to be an important mechanism of reperfusion injury. It has been proposed that C1 esterase inhibitor (C1 INH) may prevent the complement- dependent activation of polymorphonuclear leukocytes (PMNs) that occurs within postischemic myocardium. Therefore, The effect of C1 INH was examined in neutrophil dependent isolated perfused rat heart model of ischemia (I) (20 min) and reperfusion (R) (45 min). Administration of C1 INH (5 mg/Kg) to I/R hearts in the presence of PMNs $(100{\times}10^6)$ and homologous plasma improved coronary flow and preserved cardiac contractile function (p＜0.001) in comparison to those I/R hearts receiving only vehicle. In addition, C1 INH significantly (p＜0.001) reduced PMN accumulation in the ischemic myocardium as evidenced by an attenuation in myeloperoxidase activity. These findings demonstrate the C1 INH is a potent and effective cardioprotective agent inhibits leukocyte-endothelial interaction and preserves cardiac contractile function and coronary perfusion following myocardial ischemia and reperfusion.

• YAKHAK HOEJI
• /
• v.59 no.3
• /
• pp.113-119
• /
• 2015

Protein phosphorylation is one of most important post-translational modifications (PTMs) and plays an important role in regulation of protein function. Here we develop a method for a global identification of phosphopeptides and phosphorylation sites using nano-LC MS/MS. We compared two separation methods, C4 and strong cation ion exchange (SCX). Before phosphopeptides enrichment with $TiO_2$, total proteins from Rat 1 cells have been separated using C4 column or tryptic peptides of proteins from the cells have been separated using SCX column. Finally, we have detected 52 phosphorylation sites on 41 proteins from SCX method and 375 phosphorylation sites on 252 proteins from C4 method, and determined the function and localization of identified phosphoproteins using DAVID software. In particular, we showed new phosphorylation sites from membrane proteins related to various cell signaling mechanisms. This method may contribute to study global signal networks induced by various signals including ligands and drugs.

• Journal of Microbiology and Biotechnology
• /
• v.29 no.3
• /
• pp.401-409
• /
• 2019

Heat-resistant microbial hosts are required for bioprocess development using high cell density cultivations at the industrial scale. We report that the thermotolerance of Escherichia coli can be enhanced by overexpressing ybeD, which was known to encode a hypothetical protein of unknown function. In the wild-type E. coli BL21(DE3), ybeD transcription level increased over five-fold when temperature was increased from $37^{\circ}C$ to either $42^{\circ}C$ or $46^{\circ}C$. To study the function of ybeD, a deletion strain and an overexpression strain were constructed. At $46^{\circ}C$, in comparison to the wild type, the ybeD-deletion reduced cell growth half-fold, and the ybeD-overexpression promoted cell growth over two-fold. The growth enhancement by ybeD-overexpression was much more pronounced at $46^{\circ}C$ than $37^{\circ}C$. The ybeD-overexpression was also effective in other E. coli strains of MG1655, W3110, DH10B, and BW25113. These findings reveal that ybeD gene plays an important role in enduring high-temperature stress, and that ybeD-overexpression can be a prospective strategy to develop thermotolerant microbial hosts.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
• /
• v.5 no.1
• /
• pp.65-72
• /
• 1993

An experiment has been carried out to identify flow patterns in a horizontal condensing flow with R-113. Characteristics of flow patterns were determined based upon a statistical analysis of differential pressure fluctuations at an orifice. The probability density function and power spectral density function of instantaneous pressure drop curves for various flow conditions were obtained. In comparison to the results of air-water flows, the flow patterns in a condensing flow such as annular, wavy, slug and plug could be identified. The experimental data determined by this technique were compared with the flow pattern maps suggested by other investigators. The result indicates that the statistical characteristics of differential pressure fluctuations at an orifice may be a useful tool for identifying flow patterns both in condensing flows and in adiabatic two-phase flows.

• Nuclear Engineering and Technology
• /
• v.54 no.4
• /
• pp.1471-1481
• /
• 2022

This paper aims to present a new method for obtaining an analytical solution for the Kaniadakis Doppler broadening (KDB) function. Also, in this work, we report the computational efficiencies of this solution compared with the numerical one. The solution of the differential equation achieved in this paper is free of approximations and is, consequently, a more robust methodology for obtaining an analytical representation of ψ_k. Moreover, the results show an improvement in efficiency using the analytical approximation, indicating that it may be helpful in different applications that require the calculation of the deformed Doppler broadening function.

• The KIPS Transactions:PartC
• /
• v.9C no.3
• /
• pp.407-412
• /
• 2002

A FR-ATM interworking function that has FR and ATM interfaces, should guarantee the QoS of both frame relay and ATM networks. The IWF requires the policing mechanisms for FR and ATM respectively, and needs to map the traffic parameters each other. In this case, traffic parameter mapping and policing function are performed independently. In the aspect of FR traffic policing, this function degrades processor performance if it is worked by software, or includes additional hardware function. The best way to solve this is to integrate this function into ATM function because ATM is performed by well defined hardware already. In this paper we propose the integrated traffic control mechanism. That is, frames are segmented to cells first, and then policing mechanism is applied to the cells to guarantee the FR QoS. Therefore proper policing algorithm is essential to the integrated FR traffic mechanism. The evaluations are done with simulations in comparison of existing FR algorithms, ATM VBR and GFR policing algorithms, and the proposed algorithm.

• Journal of Bio-Environment Control
• /
• v.23 no.1
• /
• pp.39-42
• /
• 2014

The objective of this study was to estimate cardinal temperatures for germination of lettuce (Lactuca sativar L.) using bilinear, parabolic, and beta distribution functions. Seeds of lettuce were germinated in a growth chamber at 7 constant temperatures: 10, 14, 16, 20, 24, 28, and $32^{\circ}C$. Four replicates of 100 seeds were placed on two layers of filter paper in a 9 cm petri-dish. Radicle emergence of 1 mm was scored as germination. The time course of germination was modeled using a logistic function. These minimum, optimum, and maximum temperatures were estimated by regression of the inverse of time to 50% germination rate against the temperature gradient. In bilinear function, minimum, optimum, and maximum temperatures were $7.9^{\circ}C$, $23.3^{\circ}C$, and $28.0^{\circ}C$, respectively. In parabolic function, minimum, optimum, and maximum temperatures were $9.7^{\circ}C$, $19.5^{\circ}C$, and $29.4^{\circ}C$, respectively. In beta distribution function, minimum, optimum, and maximum temperatures were $3.7^{\circ}C$, $20.7^{\circ}C$ and $32.0^{\circ}C$, respectively. Minimum, optimum, and maximum ranges of temperatures were $3.7{\sim}9.7^{\circ}C$, $19.5{\sim}23.3^{\circ}C$, and $28.0{\sim}32.0^{\circ}C$, respectively.

• International Journal of Automotive Technology
• /
• v.6 no.6
• /
• pp.599-604
• /
• 2005

A functional suspension model is proposed as a kinematic describing function of the suspension, that represents the relative wheel displacement in polynomial form in terms of the vertical displacement of the wheel center and steering rack displacement. The relative velocity and acceleration of the wheel is represented in terms of first and second derivatives of the kinematic describing function. The system equations of motion for the full vehicle dynamic model are systematically derived by using velocity transformation method of multi-body dynamics. The comparison of test and simulation results demonstrates the validity of the proposed functional suspension modeling method. The model is computationally very efficient to achieve real-time simulation on TMS 320C6711 150 MHz DSP board of HILS (hardware-in-the-loop simulation) system for ECU (electronic control unit) evaluation of semi-active suspension.

• Journal of the Korean Society of Radiology
• /
• v.9 no.6
• /
• pp.357-362
• /
• 2015

This study was to evaluate the usefulness through the comparison of patients group and healthy control group by acquiring a variety of functional parameters index from time-activity curves of salivary gland scan using 99mTc-pertechnetate. From December 2014 to February 2015, had the targets of 30 patients with dry mouth and 10 people in healthy control group underwent the salivary gland scan. After intravenous injection of 370 MBq of 99mTc-pertechnetate, Vitamin C powder stimulation was administerd orally at 20 min and then 10 minutes were taken scan. The method of quantitative analysis was as follows, the time-activity curve was drawn after the parotid gland and submandible gland were prescribed as a region of interest, a variety of function parameters index was obtained in each position of the curve, and the patients group and the control group were compared. As for the methods applied in comparison and measurement, uptake ratio (UR), time at maximum counts (Tmax), time at minimum counts (Tmin), maximum accumulation (MA), accumulation velocity, maximum secretion (MS), maximum stimulation secretion (MSS), and secretion velocity (SV) were used. In the comparison of functional parameters index of patient group and normal group, the healthy normal group showed significant difference compared to the patient group in all indices except for the minimum radioactivity time (Tmin), and also in terms of variation over time the normal group showed significant difference compared to the patient group (p<0.05). Consequently, it was considered that the quantitative analysis that used a variety of function parameters index would be useful for evaluating the function of the salivary glands of the patients with dry mouth as an objective and standardized information.