• 제목/요약/키워드: (Light)Intensity

검색결과 2,544건 처리시간 0.028초

Effects of Light Pulse Intensity and Quencher Concentration on the Time-Dependent Fluorescence Quenching Kinetics

  • Yang Mino;Lee Sangyoub;Shin, Kook Joe;Choo Kwang Yul;Lee Duckhwan
    • Bulletin of the Korean Chemical Society
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    • 제13권3호
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    • pp.325-331
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    • 1992
  • By using the general theoretical framework proposed recently for treating the fluorescence quenching kinetics, we investigate the effect of light pulse intensity on the decay of fluorescence which follows excitation of fluorophors by the light pulse of very short but finite duration. It is seen that conventional theory breaks down when the exciting light pulse has a pulse width comparable to the fluorescent lifetime and its intensity is very high. We also find that even when the light intensity is not too high, conventional theory may fail in either of the following cases: (i) when the quencher concentration is high, (ii) when there is an attractive potential of mean force between the fluorophor and quencher, or (iii) when the energy transfer from the fluorophor to the quencher may also occur at a distance, e.g., via dipole-dipole interaction. The validity of the predictions of the present theory may thus be tested by fluorescence quenching experiments performed under such situations.

Influence of light Regime on Nitrate Reductase Activity and Organic and norganic Solute Composition of Four Sedges (Carex spp.)

  • Choo, Yeon-Sik;Roland-Albert;Song, Seung-Dal
    • Animal cells and systems
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    • 제2권4호
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    • pp.455-462
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    • 1998
  • A survey was conducted on the inorganic and organic solute patterns of plants in connection with nitrate metabolism according to different light regimes (1.9, 16.0, 91.5 $Wm^{-2}$). Besides measuring in vivo NRA, we also quantitatively analyzed ater-soluble inorganic ions, organic acids, low molecular weight carbohydrates, amino aciss and total N (% DW). Among 4 Carex species, C. pilosa is known as shade-adapted species and the others as half (C. gracilis) to full (C. rostrata & C. distans) light-adapted species. Compared to species adapted to high light intensity, shade-adapted C. pilosa showed reduced productivity under the highest light intensity. In general, nitrate and amino acid levels decreased at higher light intensity, while sugar and organic acid concentrations increased. In C. pilosa osmolality tended to rise with increasing light intensity, while in the other species it tended to fall. Under low light intensity, the drop in soluble carbohydrate contents is osmotically compensated for by an enhanced nitrate concentration. It is concluded that competition between nitrate and $CO_2$reduction for reductants and ATP from photosynthesis may have important ecological consequences for the adaptation of plants to low or high light conditions. Additionally, the patterns of ionic changes due to increased light intensities were essentially the same in all selected species, indicating similar characteristics of heir mineral ion and organic acid metabolism as well as in field-grown Carex species.

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복합레진 중합용 가시광선 광중합기의 적정 광강도 유지를 위한 임상적 고찰 (A CLINICAL STUDY ON THE MAINTENANCE OF LIGHT INTENSITY OF VISIBLE-LIGHT CURING MACHINES FOR THE POLYMERIZATION OF COMPOSITE RESINS)

  • 이동수;정태성;김신
    • 대한소아치과학회지
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    • 제28권3호
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    • pp.363-368
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    • 2001
  • 광중합형 복합레진은 일부 성분들이 강한 광학-전자기선에 노출됨으로써 경화된다. 최초의 제품들은 자외선을 이용하여 중합되었다. 초기의 이러한 제품들은 작업시간의 조절가능 기포 형성 감소, 색 안정성 향상으로 각광받았지만, 자외선의 위해 성과 얕은 중합깊이를 극복하지 못해 결국 가시광선 중합형으로 대체되었다. 가시광선 중합형 복합레진의 중합완성도는 단량체(monomer)에서 복합체(polymer)로의 변환율에 의해 결정된다. 결국 수복물의 성공여부는 조사된 가시광선의 중합능력과 밀접한 연관성을 갖는다. 이번 조사의 목적은 현재 임상(치과 병 의원)에서 사용되고 있는 광중합기의 광도를 여러 연구를 통하여 그 신뢰성이 입증된 digital radiometer를 이용해 평가하고, 3가지 기본부품의 결함을 검증해서 임상가들에게 유익한 정보를 주기 위함이다. 조사 결과, 조사 대상 광중합기 중 17.8%가 적절한 중합에 부적절한 상태에 있으며, 46.6%가 광 조사시간을 연장할 필요가 있는 것으로 드러나, 광조사기의 절반 이상이 수복물의 성공적인 중합을 위해서는, 중합시간의 연장을 필요로 하거나, 기계적 결함으로 점검을 필요로 하는 상태에 있었다. 이번 조사로, 현재 치과 병, 의원에서 사용중인 광조사기의 부품성능과 그 관리에 문제가 있음을 알 수 있다. 이러한 결과는, 최근에 이루어진 외국의 조사에서와 유사하였다. 광조사기를 이용한 수복치료의 성공과, 구입당시 수준의 적절한 품질(quality)을 유지하기 위해서는, 광조사기 관리와 부품 교체에 대한 임상가들의 이해가 필요할 것으로 보인다.

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자색고구마 캘러스배양에서 안토시아닌 생합성에 미치는 광의 영향 (Effect of Light on Anthocyanin Biosynthesis in Callus Culture of Purple Sweetpotato)

  • 박혜정;김정숙;박현용
    • Journal of Plant Biotechnology
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    • 제32권4호
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    • pp.307-311
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    • 2005
  • 자색고구마의 캘러스 배양에서 광의 조사가 안토시아닌 생합성에 미치는 효과를 실험하였다. 잎조직으로부터 자색캘러스는 $0.5\;{\mu}M$ 2,4-D가 첨가된 MS배지, 광조건에서 유도되었다. 광의 세기에 따른 효과는 암 조건에 비교하여 광조건에서 안토시아닌 생합성이 $2{\sim}3$배에 이르는 것으로 나타났으며, 약광 (3000 lux)에서 안토시아닌 생합성량이 높게 나타났다. 이러한 광 조사에 의한 안토시아닌 생합성의 촉진효과는 LED를 이용하며 실험한 결과에서 청색광을 2시간 처리한 경우 3000 lux의 백색광을 처리한 대구조에 비교하여 1.4배의 안토시아닌 생합성 향상효과를 나타냈다.

Astaxanthin Production by Haematococcus pluvialis under Various Light Intensities and Wavelengths

  • Park, Eun-Kyung;Lee, Choul-Gyun
    • Journal of Microbiology and Biotechnology
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    • 제11권6호
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    • pp.1024-1030
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    • 2001
  • The key factors for high-density Haematococcus pluvialis cultures and conditions for astaxanthin induction were examined to maximize astaxanthin production. Light intensity was found to be the most important factor, and thus experiments were found to be the most important factor, and thus experiments were carried out using different light sources and intensities. A high cell density of over 2.7 g/l was obtained at $75{\mu}E/m^2/s$, whereas a much lower cell concentration (<1.0 g/ 1) was obtained with lower light intensities $(15-30{\mu}E/m^2/s$. A high light intensity and the supplement of 470 nm photons had a more dramatic effect on the final astaxanthin concentration and per cell astaxanthin content. A maximum astaxanthin concentration of 6.5 mg/l was obtained at a light intensity of $160{\mu}E/m^2/s$, whereas only 1.3 and 0.7 mg/l were obtained at 30 and $15{\mu}E/m^2/s$, respectively. A supplement of 470 nm photons enhanced the carotenoid and chlorophyll formation.

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Photoacclimation strategies of the temperate coralline alga Corallina officinalis: a perspective on photosynthesis, calcification, photosynthetic pigment contents and growth

  • Kim, Ju-Hyoung;Lam, Sao Mai N.;Kim, Kwang Young
    • ALGAE
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    • 제28권4호
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    • pp.355-363
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    • 2013
  • The coralline alga, Corallina officinalis, is a widely distributed intertidal species in temperate coastal regions. It is usually exposed to high fluctuations of light intensity, light quality, temperature, and desiccation, all of which affect the temporal and spatial distribution as well as the morphology and the metabolism of this alga. In laboratory experiments we examined the effects of different light intensities (50, 100, and 200 ${\mu}mol$ photons $m^{-2}s^{-1}$) on photosynthesis, calcification, photosynthetic pigment contents (chlorophyll a and carotenoids), and growth rate of C. officinalis to clarify its photoacclimation strategies. Net photosynthesis, calcification and dissolution rates based on weight were not sensitive to irradiance. Although, photosynthesis and calcification did not clearly respond to light intensity, photosynthetic pigment contents were significantly lower at higher light intensities. In addition, higher irradiances induced significant enhancement of gross photosynthesis based on chlorophyll a. As a result, the specific growth rate was significantly stimulated by high light intensity. Our results suggest that photoacclimation of C. officinalis to different light conditions may be regulated to optimize growth.

Changes in nocturnal insect communities in forest-dominated landscape relevant to artificial light intensity

  • Lee, Hakbong;Cho, Yong-Chan;Jung, Sang-Woo;Kim, Yoon-Ho;Lee, Seung-Gyu
    • Journal of Ecology and Environment
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    • 제45권4호
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    • pp.218-227
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    • 2021
  • Background: Artificial light at night has recently been identified as a major factor adversely affecting global insect diversity. Here, we compared the insect diversity in Gwangneung Forest Biosphere Reserve, specifically in the Korea National Arboretum (with no artificial light at night), with that of three nearby urban sites with a gradient of artificial light at night (five locations at each site). We analyzed the effects of the artificial night lighting index, mean annual temperature, and field light intensity (lux) at night on the insect community structure. Results: The urban sites generally exhibited higher species richness and abundance as well as clear indicator species compared with the control site. The size distribution of the collected insects markedly differed between the control and the three urban sites. The abundance of herbivorous and omnivorous insects increased and decreased, respectively, with the increase in light intensity. Species richness of herbivorous and omnivorous insects was likely correlated with the field light intensity at night and artificial night lighting index, respectively. Conclusions: This study demonstrates the association between nighttime environment and marked changes in insect community structure and revealed consequent transition of ecosystem services by changes in trophic group composition.

Growth of Runner Plants Grown in a Plant Factory as Affected by Light Intensity and Container Volume

  • Park, Seon Woo;Kwack, Yurina;Chun, Changhoo
    • 원예과학기술지
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    • 제35권4호
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    • pp.439-445
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    • 2017
  • Transplant production in a plant factory with artificial lighting provides several benefits; (1) rapid and uniform transplant production, (2) high production rate per unit area, and (3) production of disease free transplants production. To improve the growth of runner plants when strawberry transplants are produced in a plant factory, we conducted two experiments to investigate (1) the effect of different light intensity for stock and runner plants on the growth of runner plants, and (2) the effect of different container volume for runner plants on their growth. When the stock and runner plants were grown under nine different light conditions composed of three different light intensities (100, 200, and $400{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPF) for each stock and runner plants, increasing the light intensity for stock plants promoted the growth of runner plants, however, the growth of runner plants was not enhanced by increasing the light intensity for runner plants under same light intensity condition for stock plants. We also cultivated runner plants using plug trays with four different container volumes (21, 34, 73, and 150 mL) for 20 days after placing the stock plants, and found that using plug trays with lager container volume did not enhance the growth of runner plants. These results indicate that providing optimal condition for stock plants, rather than the runner plants, is more important for increasing the growth of the runner plants and that the efficiency of strawberry transplant production in a plant factory can be improved by decreasing light intensity or container volume for runner plants.

현대물리학 교재의 광전효과 단원에서 빛의 세기에 관한 설명방식과 교육적 함의 (How Modern Physics Textbooks Explain Intensity of Light in Photoelectric Effect)

  • 김은선;김홍빈;이종봉;이경호
    • 과학교육연구지
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    • 제44권1호
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    • pp.112-121
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    • 2020
  • 광전효과는 빛의 입자성을 설명하는 대표적인 현상으로 고전 물리학에서 현대물리학으로의 전환을 이끈 중요한 주제이다. 중등교육 과정에서도 광전 효과를 중요하게 다루고는 있지만, '빛의 세기'에 대한 설명이 전자기파의 세기에 대한 설명과 달라서 교사나 학생들이 종종 혼란을 겪고 있다. 이에 본 연구에서는 현대물리 교재의 광전효과 단원에서 '빛의 세기'를 어떻게 설명하고 있는지 분석하였다. 널리 사용되는 10종의 현대물리 교재를 분석한 결과 '빛의 세기'가 크게 두 가지 유형으로 설명되고 있었으며, 특히 한 교재 내에서 두 가지 다른 유형의 표현이 모두 나타나기도 하였다. 연구자들은 본 연구의 결과를 바탕으로 향후 광전효과를 기술하는 데 있어서 몇 가지 유의점을 제안하였다.

분할화상분석에 의한 흐름 형태 시료의 탁도 측정 (The Turbidity Measured by Division Image Analysis in Flow Type Sample)

  • 박종호;박수행;유민수
    • 공업화학
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    • 제20권6호
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    • pp.681-684
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    • 2009
  • 흐름 형태 시료의 탁도와 레이저 산란광 밝기의 관계는 비선형적이나 탁도 시료에 따라 레이저 산란광 화상은 형태가 서로 상이하다. 레이저 산란광 화상의 패턴을 이용하면 탁도 측정이 용이할 것이다. 그러나 레이저 산란광 화상의 모든 화소의 red, green, blue intensity (intensity)로 탁도를 측정하는 것은 분석에 과도한 부하가 발생한다. 따라서 과도한 부하를 줄이기 위하여 레이저 산란광 화상 분할하였다. 분할된 레이저 산란광 화상은 탁도에 따라 형태가 서로 상이함을 알 수 있었다. 분할된 레이저 산란광 화상의 intensity와 탁도로 학습된 인공신경망으로 측정된 탁도가 실제 탁도와 선형 관계임을 알 수 있었다.