Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effect of Light on Anthocyanin Biosynthesis in Callus Culture of Purple Sweetpotato

자색고구마 캘러스배양에서 안토시아닌 생합성에 미치는 광의 영향

  • Published : 2005.12.01
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Abstract

The anthocyanin biosynthesis in callus culture of purple sweet potato (Ipomoea batatas L. cv. Borami) was investigated under growth in different light intensity and light emitting diodes (LED) treatment. Pigmented calli were induced from leaf explants cultured on MS agar medium supplemented with $0.5\;{\mu}M$ 2,4-D under light condition. The color value of these calli extracted after $2{\sim}4$ weeks of cultures was $0.4{\sim}0.5\;mg/mL$. Irradiation intensity is an important factor for anthocyanin biosynthesis. The optimal anthocyanin accumulation occurred on light intensity of 3000 lux. Light irradiation of 3000 lux and blue light treatment for 2 h resulted in a significant enhancement of anthocyanin accumulation. This value was 1.4 fold that the control.

자색고구마의 캘러스 배양에서 광의 조사가 안토시아닌 생합성에 미치는 효과를 실험하였다. 잎조직으로부터 자색캘러스는 $0.5\;{\mu}M$ 2,4-D가 첨가된 MS배지, 광조건에서 유도되었다. 광의 세기에 따른 효과는 암 조건에 비교하여 광조건에서 안토시아닌 생합성이 $2{\sim}3$배에 이르는 것으로 나타났으며, 약광 (3000 lux)에서 안토시아닌 생합성량이 높게 나타났다. 이러한 광 조사에 의한 안토시아닌 생합성의 촉진효과는 LED를 이용하며 실험한 결과에서 청색광을 2시간 처리한 경우 3000 lux의 백색광을 처리한 대구조에 비교하여 1.4배의 안토시아닌 생합성 향상효과를 나타냈다.

Keywords

References

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