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http://dx.doi.org/10.5010/JPB.2005.32.4.307

Effect of Light on Anthocyanin Biosynthesis in Callus Culture of Purple Sweetpotato  

Park Hyae-Jeong (Department of Biology, Chosun University)
Kim Jung-Suk (Department of Biology, Chosun University)
Park Hyeon-Yong (Department of Biology, Chosun University)
Publication Information
Journal of Plant Biotechnology / v.32, no.4, 2005 , pp. 307-311 More about this Journal
Abstract
The anthocyanin biosynthesis in callus culture of purple sweet potato (Ipomoea batatas L. cv. Borami) was investigated under growth in different light intensity and light emitting diodes (LED) treatment. Pigmented calli were induced from leaf explants cultured on MS agar medium supplemented with $0.5\;{\mu}M$ 2,4-D under light condition. The color value of these calli extracted after $2{\sim}4$ weeks of cultures was $0.4{\sim}0.5\;mg/mL$. Irradiation intensity is an important factor for anthocyanin biosynthesis. The optimal anthocyanin accumulation occurred on light intensity of 3000 lux. Light irradiation of 3000 lux and blue light treatment for 2 h resulted in a significant enhancement of anthocyanin accumulation. This value was 1.4 fold that the control.
Keywords
Anthocyanin; in vitro culture; light intensity;
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Times Cited By KSCI : 1  (Citation Analysis)
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1 Stapletion AE, Walbot V (1994) Flavonoids can protect maize DNA from the induction of ultraviolet radiation damage. Plant Physiol 105: 881-889   DOI
2 Takeda J (1990) Light-induced synthesis of anthocyanin in carrot cells in suspension- II. Effects of light and 2,4-D on induction and reduction of enzyme activities related to anthocyanin synthesis. J Exp Bot 41: 749-755   DOI
3 Zhang W, Curtin C, Kikuchi M, Franko C (2002) Integration of jasmonic acid and light irradiation for enhancement of anthocyanin biosynthesis in Vitis vinifera suspension cultures. Plant Sci 162 : 459-468   DOI   ScienceOn
4 Zhong J, Yoshida M, Fujiyama K, Seki T, Yoshida T (1993) Enhancement of anthocyanin production by Perilla frutescens cells in a stirred bioreactor with internal light irradiation. J Frment Bioeng 75: 299-303   DOI   ScienceOn
5 Kubasek WL, Shirley BW, McKillop A, Goodman HM, Briggs W, Ausbel FM (1992) Regulation of flavonoid biosynthetic genes in germinating Arabidopsis seedlings. Plant Cell 4: 1229-1236   DOI   ScienceOn
6 Madhavi DL, Smith MAL, Berber-Jimenez MD (1995) Expression of anthocyanins in callus cultures of cranberry (Vaccinum macrocarpa Ait.). J Food Sci 60: 351-355   DOI   ScienceOn
7 Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497   DOI
8 Narayan MS, Thimmaraju R. Bhagyalakshmi (2005) Interplay of growth regulators during solid-state and liguid batch cultivation of anthocyanin producing cell line of Dacucus carota. Process Biochem 40: 351-358   DOI   ScienceOn
9 Odake K, Terahara N, Saito N, Toki K, Honda T (1994) Chemical structure of two anthocyanins from purple sweet potato, Ipomoea batatas. Phytochem 31: 2127-2130   DOI   ScienceOn
10 Noh B, Spalding E (1998) Anion channels and the stimulation of anthocyanin accumulation by blue light in arabidopsis seedlings. Plant Physiol. 116: 503-509   DOI
11 Park HJ, Ahn YS, Jeong BC, Park HY (2003) Plant regeneration derived from leaf disk cultures in purple sweetpotato. Kor J Plant Biotech 30: 245-249   과학기술학회마을   DOI   ScienceOn
12 Rhim JW, Lee JW, Jo JS, Yeo KM (2001) Pilot plant scale extraction and concentration of purple-freshed sweetpotato anthocyanin pigment. Korean J. Food Scil. Technol. 33: 808-811
13 Sato K, Nakayama M, Shigeta J (1996) Culturing conditions affecting the production of anthocyanin in suspended cell cultures of strawberry. Plant Sci 113: 91-98   DOI   ScienceOn
14 Callebaut A, Terahara N, de Haan M, Decleire M (1997) Stability of anthocyanin composition in Ajuga reptans callus and cell suspension cultures. Plant Cell Tiss Org Cult 50: 195-201   DOI
15 Francis L (1989) Food colourants : Anthocyanins. Crit Rev Food Sci 28: 273-314   DOI   ScienceOn
16 Ghiselli A, Naridini M, Baldi A, Scaccini C (1998) Antioxidant activity of different phenolic fractions separated from an Italian red wine. J Agr Food Chem 46: 361-367   DOI   ScienceOn
17 Glaebbgen WE, Rose A, Madlung J, Koch W, Gleitz J, Seitz HU (1998) Regulation of enzymes involved in anthocyanin biosynthesis in carrot cell cultures in response to treatment with ultraviolet light and fungal elicitors. Planta 204: 490-498   DOI
18 Jackman RI, Yada RY, Tung MA, Speers RA (1993) Anthocyanina as food colorants. J Food Biochem 11: 201
19 Kamei H, Hashimoto Y, Koide T, Kojima T, Hasegawa J (1998) Anti-tumor effect of methanol extracts from red and white wines. Cancer Biother and Radiopharm 136: 447-452
20 Jeong ST, Goto-yamamoto N, Kobayashi S, Esaka M (2004) Effects of plant hormones and shading on the accumulation of anthocyanins and the expression of anthocyanin biosynthetic genes in grape berry skins. Plant Sci 167: 247-252   DOI   ScienceOn
21 Kang SY, Seeram NP, Nair MG, Bourquin LD (2003) Tart cherry anthocyanins inhibit tumor development in ApcMin mice and reduce proliferation of human colon cancer cells, Cancer Lett 194: 13-19   DOI   ScienceOn
22 Kim SJ, Rhim JW, Lee LS, Lee JS (1996) Extraction and characteristics of purple sweetpotato pigment. Kor J Food Scil Technol 28: 345-351
23 Konczak I, Yoshinaga M, Nakatani M, Yamakawa O, Terahara N (2000) Establishment and characteristics of an anthocyanin-producing cell line from sweetpotato storage root. Plant Cell Rep 19: 472-477   DOI
24 Bridle P, Timberlake CF (1997) Anthocyanins as natural food colours-selected aspects Food Chem 58: 103-109   DOI   ScienceOn
25 Blando F, Scardino AP, De Bellis L, Nicoletti I, Giovinazzo G (2005) Chracterization of in vitro anthocyanin-producing sour cherry (Prunus cera년 L.) callus culture. Food Res Int 38: 937-942   DOI   ScienceOn
26 Cevallos-Casals BA, Cisneros-Zevallos L (2004) Stability of anthocyanin-based aqueous extracts of Andean purple corn and red-freshed sweet potato compared to synthetic and natural colorants. Food Chem 86: 69-77   DOI   ScienceOn