• Title/Summary/Keyword: (L, N)-structure

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Isolation and Properties of Amino Acid Antimetabolite from Streptomyces sp. YS-943 (Streptomyces sp. YS-943균주가 생산하는 아미노산 대사길항물질의 정제와 성상)

  • 유성재;박부길
    • Microbiology and Biotechnology Letters
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    • v.23 no.1
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    • pp.81-86
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    • 1995
  • A Streptomyces strain YS-943, which produced amino acid antimetabolite, was isolated from soil. During the course of screening for new amino acid antimetabolites from the culture broth of Actinomycetes, we found that the strain produced a substance active against Gram-positive bacteria and its activity was reversed by L-methionine and L-histidine on the synthetic minimal agar medium in the culture broth.The morphological and cultural characteristics serve to identify the producing organism strain YS-943 as the genus Streptomyces. Fermentation was carried out in the synthetic medium at 28$\CIRC$C for 48 hours. The fermentation yield reached about 12 mg per liter of the broth. The YS-943 substance was obtained as white powder, mp 194$\CIRC$C and has the molecular formular of C$_{4}$H$_{8}$N$_{2}$O$_{4}$. Its structure was determined to be o-carbamyl-D-serine by spectroscopic data. It is active against some Gram-positive bacteria and reversed by L-methionine and L-histidine.

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Fundamental Structure in Simultaneous Removal for Phytoplankton and Nutrient Salt in Lakes

  • SEKI, Tatsuhiro;ISHII, Yuuichi;ISHII, Toshio;TAKI, Kazuo
    • Environmental Engineering Research
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    • v.14 no.4
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    • pp.220-225
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    • 2009
  • The water quality in eutrophic lakes is affected by serious problems, such as abnormal increasing of Cyanobacteria. The purpose of this study was to investigate the possibility of a modified flotation system using a hybrid technique formed by chemical compounds and an electrostatic bridge. Therefore, experiments using the hybrid technique were performed to measure the zeta potential value on the phytoplankton surface and the removal efficiencies of phytoplankton, ammonia nitrogen, nitrate nitrogen and phosphoric acid. The results were as follows: Firstly, the zeta potential of M.aeruginosa was observed to approach charge neutralization due to adhesion of magnesium hydroxide precipitate on the phytoplankton surface in the pH range 10.5 to 11. Secondly, the concentration of chlorophyll-a decreased from about 150 to 20${\mu}g$g/L, with a maximum removal efficiency of 84% due to coagulation with pH values higher than 10. Thirdly, the N$H_4$-N concentration was observed to decrease from 0.62 to 0.54mg-N/L (13%), and the P$O_4$-P concentration, which is a limiting factor to the formation of algae blooms, decreased from 0.27 to 0.02mg-P/L (92%). These findings suggest that the modified flotation system can be applied for the purification of the raw water of numerous lakes containing high phytoplankton populations and elevated pH.

Aspects of Korean rhythm realization by second language learners: Focusing on Chinese learners of Korean (제 2언어 학습자의 한국어 리듬 실현양상 -중국인 한국어 학습자를 중심으로-)

  • Youngsook Yune
    • Phonetics and Speech Sciences
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    • v.15 no.3
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    • pp.27-35
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    • 2023
  • This study aimed to investigate the effect of Chinese on the production of Korean rhythm. Korean and Chinese are typologically classified into different rhythmic categories; because of this, the phonological properties of Korean and Chinese are similar and different at the same time. As a result, Chinese can exert both positive and negative influences on the realization of Korean rhythm. To investigate the influence of the rhythm of the native language of L2 learners on their target language, we conducted an acoustic analysis using acoustic metrics like of the speech of 5 Korean native speakers and 10 advanced Chinese Korean learners. The analyzed material is a short paragraph of five sentences containing a variety of syllable structures. The results showed that KS and CS rhythms are similar in %V, VarcoV, and nPVI_S. However, CS, unlike KS, showed characteristics closer to those of a stress-timed language in the values of %V and VarcoV. There was also a significant difference in nPVI_V values. These results demonstrate a negative influence of the native language in the realization of Korean rhythm. This can be attributed to the fact that all vowels in Chinese sentence are not pronounced with the same emphasis due to neutral tone. In this sense, this study allowed us to observe influences of L1 on L2 production of rhythm.

CO-CLUSTER HOMOTOPY QUEUING MODEL IN NONLINEAR ALGEBRAIC TOPOLOGICAL STRUCTURE FOR IMPROVING POISON DISTRIBUTION NETWORK COMMUNICATION

  • V. RAJESWARI;T. NITHIYA
    • Journal of applied mathematics & informatics
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    • v.41 no.4
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    • pp.861-868
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    • 2023
  • Nonlinear network creates complex homotopy structural communication in wireless network medium because of complex distribution approach. Due to this multicast topological connection structure, the queuing probability was non regular principles to create routing structures. To resolve this problem, we propose a Co-cluster homotopy queuing model (Co-CHQT) for Nonlinear Algebraic Topological Structure (NLTS-) for improving poison distribution network communication. Initially this collects the routing propagation based on Nonlinear Distance Theory (NLDT) to estimate the nearest neighbor network nodes undernon linear at x(a,b)→ax2+bx2 = c. Then Quillen Network Decomposition Theorem (QNDT) was applied to sustain the non-regular routing propagation to create cluster path. Each cluster be form with co variance structure based on Two unicast 2(n+1)-Z2(n+1)-Z network. Based on the poison distribution theory X(a,b) ≠ µ(C), at number of distribution routing strategies weights are estimated based on node response rate. Deriving shorte;'l/st path from behavioral of the node response, Hilbert -Krylov subspace clustering estimates the Cluster Head (CH) to the routing head. This solves the approximation routing strategy from the nonlinear communication depending on Max- equivalence theory (Max-T). This proposed system improves communication to construction topological cluster based on optimized level to produce better performance in distance theory, throughput latency in non-variation delay tolerant.

NMR Studies on the Structure of Human Annexin I

  • Lee, Yeon-Hee;Han, Hee-yong;Oh, Jee-Young;Na, Doe-Sun;Lee, Bong-Jin
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1997.04a
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    • pp.86-86
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    • 1997
  • Human annexin I is a member of annexin family of calcium dependent phospholipid binding proteins, which have been implicated in various physiological roles including phospholipase A$_2$ (PLA$_2$) inhibition, membrane fusion and calcium channel activity. In this work, the structure of N-terminally truncated human annexin I (Δ-annexin I) and its interactions with Ca$\^$2+/, ATP and cAMP were studied at atomic level by using $^1$H, $\^$15/N, $\^$l3/C NMR (nuclear magnetic resonance) spectroscopy. The effect of Ca$\^$2+/ binding on the structure of Δ-annexin I was investigated, and compared with that of Mg$\^$2+/ binding. The addition of Ca$\^$2+/ to Δ-annexin I caused some changes in the high field and low field regions of $^1$H NMR spectra. Whereas, upon addition of Mg$\^$2+/ to Δ-annexin I, almost no change could be observed. Also we found that the binding ratio of ATP to Δ-annexin I is 1. Because Δ-annexin I is a large protein with 35 kDa molecular weight, site-specific (carbonyl-$\^$l3/C, amide-$\^$15/N) labeling technique was used to determine the interaction sites of Δ-annexin I with Ca$\^$2+/ and ATP. Assignments of all the histidinyl carbonyl carbon resonances have been completed by using Δ-annexin I along with its specific 1,2-subdomain. The carbonyl carbon resonances originating from His52 and His246 of Δ-annexin I were significantly affected by Ca$\^$2+/ binding, and some Tyr and Phe resonances were also affected. The carbonyl carbon resonances originating from His52 is significantly affected by ATP binding, therefore His52 seems to be involved in the ATP binding site of Δ-annexin I.

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Solid Phase Synthesis of N-(3-hydroxysulfonyl)-L-homoserine Lactone Derivatives and their Inhibitory Effects on Quorum Sensing Regulation in Vibrio harveyi (고체상 합성법에 의해 합성된 N-(3-hydroxysulfonyl)-L-homoserine Lactone 유사체들의 Vibrio harveyi 쿼럼 센싱에 대한 저해 효과)

  • Kim, Cheol-Jin;Park, Hyung-Yeon;Kim, Jae-Eun;Park, Hee-Jin;Lee, Bon-Su;Choi, Yu-Sang;Lee, Joon-Hee;Yoon, Je-Yong
    • Microbiology and Biotechnology Letters
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    • v.37 no.3
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    • pp.248-257
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    • 2009
  • The inhibitors against Vibrio harveyi quorum sensing (QS) signaling were developed by modifying the molecular structure of the major signal, N-3-hydroxybutanoyl-L-homoserine lactone (3-OH-$C_4$-HSL). A series of structural derivatives, N-(3-hydroxysulfonyl)-L-homoserine lactones (HSHLs) were synthesized by the solid-phase organic synthesis method. The in vivo QS inhibition by these compounds was measured by a bioassay system using the V. harveyi bioluminescence, and all showed significant inhibitory effects. To analyze the interaction between these compounds and LuxN, a 3-OH-$C_4$-HSL receptor protein of V. harveyi, we tentatively determined the putative signal binding domain of LuxN based on the sequence homology with other acyl-HSL binding proteins, and predicted the partial 3-D structure of the putative signal binding domain of LuxN by using ORCHESTRA program, and further estimated the binding poses and energies (docking scores) of 3-OH-$C_4$-HSL and HSHLs within the domain. In comparison of the result from this modeling study with that of in vivo bioassay, we suggest that the in silica interpretation of the interaction between ligands and their receptor proteins can be a valuable way to develop better competitive inhibitors, especially in the case that the structural information of the protein is limited.

The Object Extraction by the Inverse-Mother-Son-Varoance Ratio and the Top-down Method (역모자분산화와 톱 - 다운 방법을 이용한 물체추출)

  • 한수용;최성진;김춘길
    • Journal of the Korean Institute of Telematics and Electronics B
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    • v.28B no.7
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    • pp.566-577
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    • 1991
  • In this paper, the method of image segmentation based on a pyramid of reduced resolution versions of the input input image is persented. In a pyramid structure, two regions (a given pixel and its mother pixels) are compared by the proposed inverse-mother-son variance ratio (IMSVR) method for the detection of an optinal object pixel and are determined whether they are similar enough to be viewed as one region or disparate to be viewed as ditinct regions By the proposed method, an l`timal object pixel has been setectedat some level, it is necessary to retrieve its boundary precisely. Moving down the pyramid to levels of higher resolution is requires. In this paper, the top-sown pyramid traversing algorithm for an image segmentation using a pyrmid structure is presented. Using the computer simulation, the results by the proposed statistical method and object traversing method are investigated for the binary image and the real image at the results of computer simulation, the proposed method of image segmentation based on a pyramid structure seem to have useful properties and deserve consideration as a possible alternative to existing methods of omage segmentation. The computation for the proposed method is required 0 (log n), for an TEX>$n{\times}n$ input image.

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A New type $1.0\;mm\;{\times}\;0.5mm$ Light Emitting Diode using AlInGaN cell structure and Its Display Module

  • Park, Book-Sung;Kim, Sung-Woon;Lee, Seon-Gu;Son, Sung-Il;Kim, Eun-Tae;Kim, Chul-Ju
    • 한국정보디스플레이학회:학술대회논문집
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    • 2008.10a
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    • pp.557-560
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    • 2008
  • The main goal of this work is to fabricate light emitting diode (LED) module and apply it to mobile handset. We first fabricated the blue-color LED based on the AlInGaN cell structure with size of $200\;{\mu}m\;{\times}\;200\;{\mu}m$. Also we proposed a new $1.0\;mm\;{\times}\;0.5\;mm$ (1005size) packaging procedure for the LED cell. Thus the overall dimension of our LED cell was as small as $1.0\;mm\;{\times}\;0.5\;mm\;{\times}\;0.4\;mm$ ($W\;{\times}\;L\;{\times}\;T$). As far as we knew it was the first time that this small LED cell dimension had been fabricated and operated.

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