• Journal of Oral Medicine and Pain
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• 제44권1호
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• pp.25-30
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• 2019

Purpose: The exact causes of burning mouth syndrome (BMS) is unclear so far. There are many studies to elucidate the relation between oral disease and genetic predisposition. In this study, we first tried to investigate salivary exosomal genetic components that could play an important role for diagnosing and elucidating the progression of BMS. Methods: We compared salivary exosomal micro RNAs (miRNAs) of BMS Patients to those of control using next generation sequencing (NGS). Unstimulated whole saliva from 15 patients with BMS and 10 control subjects were divided into two sets. Isolated exosomes and their total RNAs were subject to NGS for the screening of miRNAs. Results: There were up-regulated 10 exosomal miRNAs (hsa-miR-1273h-5p, hsa-miR-1273a, hsa-miR-1304-3p, hsa-miR-4449, hsa-miR-1285-3p, hsa-miR-6802-5p, hsa-miR-1268a, hsa-miR-1273d, hsa-miR-1273f, and hsa-miR-423-5p) and down-regulated 18 exosomal miRNAs (hsa-miR-27b-3p, hsa-miR-16-5p, hsa-miR-186-5p, hsa-miR-142-3p, hsa-miR-141-3p, hsa-miR-150-5p, hsa-miR-374a-5p, hsa-miR-93-5p, hsa-miR-29c-3p, hsa-miR-29a-3p, hsa-miR-148a-3p, hsa-miR-22-3p, hsa-miR-27a-3p, hsa-miR-424-5p, hsa-miR-19b-3p, hsa-miR-99a-5p, hsa-miR-548d-3p, and hsa-miR-19a-3p) in BMS patients comparing with those of control subjects. Conclusions: We show that there are 28 differential expression of miRNAs between the patients with BMS and those of control subjects. The specific function of indicated miRNAs should be further elucidated.

• Journal of Gastric Cancer
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• 제19권3호
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• pp.315-328
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• 2019

Purpose: To investigate the clinical value of serum miR-221-3p and miR-122-5p expression levels in the diagnosis and prognosis of gastric cancer. Materials and Methods: Serum samples from 141 gastric cancer cases (gastric cancer group), 110 gastric polyps (gastric polyp group), and 75 healthy people (healthy control) were used to detect miR-221-3p and miR-122-5p expression using real-time reverse transcription polymerase chain reaction. Results: Serum miR-221-3p expression was significantly higher in the gastric cancer group than in the gastric polyp group, and it was significantly lower than that before operation. The miR-221-3p expression was significantly higher in the death group than in the survival group. The proliferation and migration ability significantly increased and the apoptosis rate significantly decreased by miR-221-3p transfection in gastric cancer cells. In contrast, the function of miR-122-5p in gastric cancer cells was opposite of miR-221-3p. Serum miR-221-3p expression was negatively correlated with that of miR-122-5p in gastric cancer. Serum miR-221-3p and miR-122-5p expressions were significantly correlated with the degree of differentiation, tumor, node, metastasis stage, lymph node metastasis, and invasion depth. miR-221-3p and miR-122-5p expression levels were independent prognostic factors for postoperative gastric cancer. In the diagnosis and predicting prognosis of gastric cancer, receiver operating characteristic analysis revealed that the area under curve of combined detection of serum miR-221-3p and miR-122-5p expression had a greater diagnostic effect than either single maker. Conclusions: The miR-221-3p and miR-122-5p are involved in the development of gastric cancer, and they have important clinical values in gastric cancer diagnosis and prognosis.

• 지역사회간호학회지
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• 제1권1호
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• pp.503-517
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• 1989

This study is to analyze factors affecting the school nurse's activities. The survey was undertaken during Sept. 1-Nov. 30, 1986. The subjects were 137 school nurses from elementary, junior-high, and senior-high schools in Daegu City and Kyungppk Province. The results are as follows: 1. Correlational findings between school nurse's self-confidence and their general characteristics 1) Program Planning & Evaluation: Health Experinece(r=-0.1803, p<0.05) Salary Step(r=-0.1741, p<0.05) 2) Clinic Management: Salary STep(r=-0.2580, p<0.01) 3) Health Education: Salary Step(r=-0.1929, p<0.05) 4) Management of School Environment: Salary Step(r=-0.2501, p<.05) 5) Health Care Services: Health Experience(r=0.1901, p<0.05) Salary Step(r=-0.2424, p<0.05) 2. The degrees of school nurse's self-confidence(high: 4 point, low: 1 point) 1) Clinic Management: 2.92 2) Health Education: 2.86. 3) Program Planning & Evaluation: 2.74 4) Health Care Services: 2.73 5) Management of School Environment: 2.67 6) Operating of School Health Organization: 2.42 3. Significances to self-confidence on school nurse's activities 1) Program Planning as Evaluation: Expending Times for Health Care Services (r=-0.2262, p<0.05) Expending Times for Health Education (r=0.2943, p<0.05) Size of Clinic(r=0.2163, p<0.05) Location of Clinic(t=2.43, gH0.047) Use of Clinic(t=2.06, p<0.007) 2) Clinic Management: Location of Clinic (t=3.36, p<0.010) 3) Health Education: Purchase of Medicine(r=-0.1736, p<0.05) No, of Classes (r=-0.1794, p<0.05) (4) Management of School Environment: School Health Budget(r=0.1731, p<0.05) Home Message(r=0.1805, p<0.05) Location of Clinic(t=4.46, p<0.0001) 5) Operating of School Health Organization: School Health Budget(r=0.1878, p<0.05) Use of Clinic(t:1.90, p<0.018) 6) Health Care Services: School Health Budget(r=1.90, p<0.018) Expending Times for Health Education(r=0.2577, p<0.05) Size of Clinic(r=0.4336, p<0.001) Location of Clinic(t:5.10, p<0.001)

• 한국미생물·생명공학회지
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• 제31권2호
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• pp.103-110
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• 2003

그람 양성세균에서 PyrR단백질에 의하여 피리미딘의 생합성이 조절된다는 발견을 바탕으로 하여, Synechocystis sp.PCC6803과 Haemophilus influenzae의 PyrR orthologue 유전자를 Bacillus subtilis에서 형질전환 시켜 피리미딘 생합성의 조절 유무를 조사하였다. Synechocystis sp.PCC6803과 H. influenzae의 PyrR orthologue유전자를 pUC19과 T-vector에 클로닝 한후 pKH1, pKH2, pHPSK1, pHPSK2으로 각각 명명하였다. 이것을 다시 Escherichia. coli와 B. subtiius용 shuttle vector인 pHPS9에 클로닝 하여 pKH3, pKH4, pHPSK3, pHPSK4로 각각 명명하였다. B. subtilis DB104Δ PyrR에 pKH3, pKH4, pHPSK3, pHPSK4을 형질전환후 ATCase 활성을 측정결과 pHPSK3을 가진 균주만 피리미딘에 의한 조절작용이 일어난다는 사실을 통하여, H. influenzae의 PyrR orthologue 유전자의 선도 부분에 조절에 관여하는 미지의 부분이 있음을 예측할 수 있었다. 서로 다른 유래의 PyrR orthologue단백질을 정제하기 위하여 pET14b에 클로닝후 pKH5, pHPSK5으로 각각 명명하였다. SDS-PAGE로 분석한 결과 각각 약 18 kDa과 21 kDa의 분자량을 나타내었다. 정제된 PyrR orthologue 단백질의 UPRTase 활성을 측정한 결과 H. infuenzae의 PyrR orthologue 단백질은 UPRTase 활성을 나타내었으며 다양한 pH에서 측정한 결과 pH 5에서 가장 높은 활성을 나타내었다. 반면에, Synechocystis sp. PCC6803의 PyrR orhologue 단백질은 UPRTase 활성을 나타내지 않았다. 여러 가지 균주의 PyrR 아미노산 서열을 비교한 계통수 분석은 PyrR 단백질의 조절기작과 어느 정도 연관됨을 시사해 주었다.

• East Asian mathematical journal
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• 제17권2호
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• pp.275-286
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• 2001

In this paper, a skew polynomial ring $R[x;\alpha]$ of a ring R with a monomorphism $\alpha$ are investigated as follows: For a reduced ring R, assume that $\alpha(P){\subseteq}P$ for any minimal prime ideal P in R. Then (i) $R[x;\alpha]$ is a reduced ring, (ii) a ring R is Baer(resp. quasi-Baer, p.q.-Baer, a p.p.-ring) if and only if the skew polynomial ring $R[x;\alpha]$ is Baer(resp. quasi-Baer, p.q.-Baer, a p.p.-ring).

• 대한수학회보
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• 제46권6호
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• pp.1041-1050
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• 2009

Let R be a ring and ${\alpha}$ a monomorphism of R. We study the skew Laurent polynomial rings R[x, x$^{-1}$; ${\alpha}$] over an ${\alpha}$-skew Armendariz ring R. We show that, if R is an ${\alpha}$-skew Armendariz ring, then R is a Baer (resp. p.p.-)ring if and only if R[x, x$^{-1}$; ${\alpha}$] is a Baer (resp. p.p.-) ring. Consequently, if R is an Armendariz ring, then R is a Baer (resp. p.p.-)ring if and only if R[x, x$^{-1}$] is a Baer (resp. p.p.-)ring.

• 대한핵의학회:학술대회논문집
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• 대한핵의학회 2000년도 2nd Korea-China Congress of Nuclear Medicine and 39th Annual Spring Meeting if the Korea SOciety Nuclear Medicine
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• pp.58.2-58.2
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• 2000

• 한국산학기술학회논문지
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• 제13권3호
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• pp.1220-1228
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• 2012

본 연구는 치과위생사가 경험하는 직무 스트레스 정도와 직무만족도 및 상관관계를 파악 하여 치과위생사 직무만족도를 높여 양질의 의료서비스를 제공하고, 치과위생사 개인의 발전과 건강 증진에 필요한 기초자료를 마련하고자 본 연구를 시행하였다. 2011년 8월 1일부터 12일까지 전국(서울, 경기, 대구, 포항)의 종합병원, 치과병원, 치과의원의 치과위생사 210명을 대상으로 자기기입식 설문지 조사를 시행한 후 205명에 대해서 분석 하였다. 수집된 자료는 SPSS WIN 15.0 프로그램을 이용하여 분석하였다. 그 결과 직무스트레스 중에는 평균이 '직무요구'가 2.71로 가장 높게 나타났다. 치과위생사의 빌딩관련 건강자각도와 직무스트레스의 영역별 관계에 대해 살펴본 결과는 중추신경계 증상은 물리환경(r=.281, p<.001)과 직무요구(r=.281, p<.001), 관계갈등(r=.163, p<.05), 조직체계(r=.166, p<.05), 보상 부적절(r=.267, p<.001), 그리고 직무스트레스(r=.311, p<.001)와 통계적으로 유의미한 정적 상관관계를 보였다. 전체적으로 빌딩관련 건강자각도는 물리환경(r=.259, p<.001)과 직무요구(r=.311, p<.001), 관계갈등(r=.172, p<.05), 조직체계(r=.158, p<.05), 보상 부적절(r=.197, p<.01), 그리고 직무스트레스(r=.275, p<.001)와 통계적으로 유의한 상관관계를 보였다. 치과위생사에 대한 근무환경 중 실내공기오염저감 및 스트레스를 예방할 수 있는 근무환경 개선의 기초자료로 활용함으로써 치과위생사의 정신건강 및 삶의 질 향상으로 양질의 의료서비스 제공에 기여할 수 있을 것으로 판단된다.

• East Asian mathematical journal
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• 제18권2호
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• pp.271-282
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• 2002

Let R be a ring with an endomorphism $\sigma$ and a derivation $\delta$. An ideal I of R is ($\sigma,\;\delta$)-ideal of R if $\sigma(I){\subseteq}I$ and $\delta(I){\subseteq}I$. An ideal P of R is a ($\sigma,\;\delta$)-prime ideal of R if P(${\neq}R$) is a ($\sigma,\;\delta$)-ideal and for ($\sigma,\;\delta$)-ideals I and J of R, $IJ{\subseteq}P$ implies that $I{\subseteq}P$ or $J{\subseteq}P$. An ideal Q of R is ($\sigma,\;\delta$)-semiprime ideal of R if Q is a ($\sigma,\;\delta$)-ideal and for ($\sigma,\;\delta$)-ideal I of R, $I^2{\subseteq}Q$ implies that $I{\subseteq}Q$. The ($\sigma,\;\delta$)-prime radical (resp. prime radical) is defined by the intersection of all ($\sigma,\;\delta$)-prime ideals (resp. prime ideals) of R and is denoted by $P_{(\sigma,\delta)}(R)$(resp. P(R)). In this paper, the following results are obtained: (1) $P_{(\sigma,\delta)}(R)$ is the smallest ($\sigma,\;\delta$)-semiprime ideal of R; (2) For every extended endomorphism $\bar{\sigma}$ of $\sigma$, the $\bar{\sigma}$-prime radical of an Ore extension $P(R[x;\sigma,\delta])$ is equal to $P_{\sigma,\delta}(R)[x;\sigma,\delta]$.

• BMB Reports
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• 제55권9호
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• pp.447-452
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• 2022

Neurogenic differentiation 1 (NeuroD1) is an essential transcription factor for neuronal differentiation, maturation, and survival, and is associated with inflammation in lipopolysaccharide (LPS)-induced glial cells; however, the concrete mechanisms are still ambiguous. Therefore, we investigated whether NeuroD1-targeting miRNAs affect inflammation and neuronal apoptosis, as well as the underlying mechanism. First, we confirmed that miR-30a-5p and miR-153-3p, which target NeuroD1, reduced NeuroD1 expression in microglia and astrocytes. In LPS-induced microglia, miR-30a-5p and miR-153-3p suppressed pro-inflammatory cytokines, reactive oxygen species, the phosphorylation of c-Jun N-terminal kinase, extracellular-signal-regulated kinase (ERK), and p38, and the expression of cyclooxygenase and inducible nitric oxide synthase (iNOS) via the NF-κB pathway. Moreover, miR-30a-5p and miR-153-3p inhibited the expression of NOD-like receptor pyrin domain containing 3 (NLRP3) inflammasomes, NLRP3, cleaved caspase-1, and IL-1β, which are involved in the innate immune response. In LPS-induced astrocytes, miR-30a-5p and miR-153-3p reduced ERK phosphorylation and iNOS expression via the STAT-3 pathway. Notably, miR-30a-5p exerted greater anti-inflammatory effects than miR-153-3p. Together, these results indicate that miR-30a-5p and miR-153-3p inhibit MAPK/NF-κB pathway in microglia as well as ERK/STAT-3 pathway in astrocytes to reduce LPS-induced neuronal apoptosis. This study highlights the importance of NeuroD1 in microglia and astrocytes neuroinflammation and suggests that it can be regulated by miR-30a-5p and miR-153-3p.