• Journal of Oral Medicine and Pain
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• v.44 no.1
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• pp.25-30
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• 2019

Purpose: The exact causes of burning mouth syndrome (BMS) is unclear so far. There are many studies to elucidate the relation between oral disease and genetic predisposition. In this study, we first tried to investigate salivary exosomal genetic components that could play an important role for diagnosing and elucidating the progression of BMS. Methods: We compared salivary exosomal micro RNAs (miRNAs) of BMS Patients to those of control using next generation sequencing (NGS). Unstimulated whole saliva from 15 patients with BMS and 10 control subjects were divided into two sets. Isolated exosomes and their total RNAs were subject to NGS for the screening of miRNAs. Results: There were up-regulated 10 exosomal miRNAs (hsa-miR-1273h-5p, hsa-miR-1273a, hsa-miR-1304-3p, hsa-miR-4449, hsa-miR-1285-3p, hsa-miR-6802-5p, hsa-miR-1268a, hsa-miR-1273d, hsa-miR-1273f, and hsa-miR-423-5p) and down-regulated 18 exosomal miRNAs (hsa-miR-27b-3p, hsa-miR-16-5p, hsa-miR-186-5p, hsa-miR-142-3p, hsa-miR-141-3p, hsa-miR-150-5p, hsa-miR-374a-5p, hsa-miR-93-5p, hsa-miR-29c-3p, hsa-miR-29a-3p, hsa-miR-148a-3p, hsa-miR-22-3p, hsa-miR-27a-3p, hsa-miR-424-5p, hsa-miR-19b-3p, hsa-miR-99a-5p, hsa-miR-548d-3p, and hsa-miR-19a-3p) in BMS patients comparing with those of control subjects. Conclusions: We show that there are 28 differential expression of miRNAs between the patients with BMS and those of control subjects. The specific function of indicated miRNAs should be further elucidated.

• Journal of Gastric Cancer
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• v.19 no.3
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• pp.315-328
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• 2019

Purpose: To investigate the clinical value of serum miR-221-3p and miR-122-5p expression levels in the diagnosis and prognosis of gastric cancer. Materials and Methods: Serum samples from 141 gastric cancer cases (gastric cancer group), 110 gastric polyps (gastric polyp group), and 75 healthy people (healthy control) were used to detect miR-221-3p and miR-122-5p expression using real-time reverse transcription polymerase chain reaction. Results: Serum miR-221-3p expression was significantly higher in the gastric cancer group than in the gastric polyp group, and it was significantly lower than that before operation. The miR-221-3p expression was significantly higher in the death group than in the survival group. The proliferation and migration ability significantly increased and the apoptosis rate significantly decreased by miR-221-3p transfection in gastric cancer cells. In contrast, the function of miR-122-5p in gastric cancer cells was opposite of miR-221-3p. Serum miR-221-3p expression was negatively correlated with that of miR-122-5p in gastric cancer. Serum miR-221-3p and miR-122-5p expressions were significantly correlated with the degree of differentiation, tumor, node, metastasis stage, lymph node metastasis, and invasion depth. miR-221-3p and miR-122-5p expression levels were independent prognostic factors for postoperative gastric cancer. In the diagnosis and predicting prognosis of gastric cancer, receiver operating characteristic analysis revealed that the area under curve of combined detection of serum miR-221-3p and miR-122-5p expression had a greater diagnostic effect than either single maker. Conclusions: The miR-221-3p and miR-122-5p are involved in the development of gastric cancer, and they have important clinical values in gastric cancer diagnosis and prognosis.

• Research in Community and Public Health Nursing
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• v.1 no.1
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• pp.503-517
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• 1989

This study is to analyze factors affecting the school nurse's activities. The survey was undertaken during Sept. 1-Nov. 30, 1986. The subjects were 137 school nurses from elementary, junior-high, and senior-high schools in Daegu City and Kyungppk Province. The results are as follows: 1. Correlational findings between school nurse's self-confidence and their general characteristics 1) Program Planning & Evaluation: Health Experinece(r=-0.1803, p<0.05) Salary Step(r=-0.1741, p<0.05) 2) Clinic Management: Salary STep(r=-0.2580, p<0.01) 3) Health Education: Salary Step(r=-0.1929, p<0.05) 4) Management of School Environment: Salary Step(r=-0.2501, p<.05) 5) Health Care Services: Health Experience(r=0.1901, p<0.05) Salary Step(r=-0.2424, p<0.05) 2. The degrees of school nurse's self-confidence(high: 4 point, low: 1 point) 1) Clinic Management: 2.92 2) Health Education: 2.86. 3) Program Planning & Evaluation: 2.74 4) Health Care Services: 2.73 5) Management of School Environment: 2.67 6) Operating of School Health Organization: 2.42 3. Significances to self-confidence on school nurse's activities 1) Program Planning as Evaluation: Expending Times for Health Care Services (r=-0.2262, p<0.05) Expending Times for Health Education (r=0.2943, p<0.05) Size of Clinic(r=0.2163, p<0.05) Location of Clinic(t=2.43, gH0.047) Use of Clinic(t=2.06, p<0.007) 2) Clinic Management: Location of Clinic (t=3.36, p<0.010) 3) Health Education: Purchase of Medicine(r=-0.1736, p<0.05) No, of Classes (r=-0.1794, p<0.05) (4) Management of School Environment: School Health Budget(r=0.1731, p<0.05) Home Message(r=0.1805, p<0.05) Location of Clinic(t=4.46, p<0.0001) 5) Operating of School Health Organization: School Health Budget(r=0.1878, p<0.05) Use of Clinic(t:1.90, p<0.018) 6) Health Care Services: School Health Budget(r=1.90, p<0.018) Expending Times for Health Education(r=0.2577, p<0.05) Size of Clinic(r=0.4336, p<0.001) Location of Clinic(t:5.10, p<0.001)

• Microbiology and Biotechnology Letters
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• v.31 no.2
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• pp.103-110
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• 2003

The regulation of pyrimidine nucleotide synthesis has been proved to be controlled by a regulatory protein PyrR-mediated attenuation in the Gram-positive bacteria. After several bacterial genome sequencing projects, we have discovered the PyrR orthologues in the databases for Haemophilus influenzae and Synechocystis and sp. PCC6803 genome sequences. To investigate whether these PyrR orthologue proteins regulate pyrimidine nucleotide synthesis as well as the cases of Bacillus, the PyrR regions of each strains were amplified by PCR and cloned with pUC19 or T-vector in Escherichia coli and with a shuttle vector pHPS9 for E. coli and B. subtilis. For the regulation test of the PyrR orthologues, the aspartate-transcarbamylase (ATCase) assay was carried out. From the results of the ATCase assay, it was confirmed that Synechocystis sp. PCC6803 could not restore by pyrimidines to a B. subtilis, PyrR but H. influenzae PyrR could. For Purification of PyrR orthologue proteins, PyrR orthologue genes were cloned into the expression vector (pET14b). Over-expressed product of PyrR orthologue genes was purified and analyzed by the SDS-PACE. The purified PyrR orthologue proteins from H. influenzae and Synechocystis sp. PCC6803 turned out to be molecular mass of 18 kDa and 21 kDa, respectively. The result of uracil phosphoribosyl transferase (UPRTase) assay with purified PyrR orthologue proteins showed that H. influenzae PyrR protein only has UPRTase activity. In addition, we could predict several regulatory mechanisms that PyrR orthologue proteins regulate pyrimidine de novo synthesis in bacteria, through phylogenetic analysis for PyrR orthologue protein sequences.

• East Asian mathematical journal
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• v.17 no.2
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• pp.275-286
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• 2001

In this paper, a skew polynomial ring $R[x;\alpha]$ of a ring R with a monomorphism $\alpha$ are investigated as follows: For a reduced ring R, assume that $\alpha(P){\subseteq}P$ for any minimal prime ideal P in R. Then (i) $R[x;\alpha]$ is a reduced ring, (ii) a ring R is Baer(resp. quasi-Baer, p.q.-Baer, a p.p.-ring) if and only if the skew polynomial ring $R[x;\alpha]$ is Baer(resp. quasi-Baer, p.q.-Baer, a p.p.-ring).

• Bulletin of the Korean Mathematical Society
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• v.46 no.6
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• pp.1041-1050
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• 2009

Let R be a ring and ${\alpha}$ a monomorphism of R. We study the skew Laurent polynomial rings R[x, x$^{-1}$; ${\alpha}$] over an ${\alpha}$-skew Armendariz ring R. We show that, if R is an ${\alpha}$-skew Armendariz ring, then R is a Baer (resp. p.p.-)ring if and only if R[x, x$^{-1}$; ${\alpha}$] is a Baer (resp. p.p.-) ring. Consequently, if R is an Armendariz ring, then R is a Baer (resp. p.p.-)ring if and only if R[x, x$^{-1}$] is a Baer (resp. p.p.-)ring.

• 대한핵의학회:학술대회논문집
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• 2000.05a
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• pp.58.2-58.2
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• 2000

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.3
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• pp.1220-1228
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• 2012

This study was carried out in order to offer medical service in good quality by enhancing dental hygienists' job satisfaction and to arrange basic data necessary for individual development and health promotion in dental hygienists through grasping the job stress level and job satisfaction of being experienced by dental hygienists, and the correlation. The self-administered questionnaire was surveyed and collected from August 1-12 of 2011 targeting 210 dental hygienists of general hospitals, dental hospitals, and dental clinics nationwide(Seoul, Gyeonggi, Daegu, Pohang).The analysis was made on 205 people. The collected data was analyzed by using SPSS WIN 15.0 program. As a result, out of job stress, the average was indicated to be the highest with 2.71 in 'job demand.' As a result of examining about sphere-based relationship between health perception level and job stress related to building in dental hygienists, the central nervous system manifestation showed statistically significant positive correlation with physical environment(r=.281, p<.001), job demand(r=.281, p<.001), relational conflict(r=.163, p<.05), organizational system(r=.166, p<.05), compensation inappropriateness(r=.267, p<.001), and job stress(r=.311, p<.001). Overall, the building-related health perception level showed statistically significant correlation with physical environment(r=.259, p<.001), job demand(r=.311, p<.001), relational conflict(r=.172, p<.05), organizational system(r=.158, p<.05), compensation inappropriateness(r=.197, p<.01), and job stress(r=.275, p<.001). They need to improve their quality of life and life and mental health by developing proper air conditioning system and using one in their work place. if so, they could contribute to better quality of oral cavity service for everyone.

• East Asian mathematical journal
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• v.18 no.2
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• pp.271-282
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• 2002

Let R be a ring with an endomorphism $\sigma$ and a derivation $\delta$. An ideal I of R is ($\sigma,\;\delta$)-ideal of R if $\sigma(I){\subseteq}I$ and $\delta(I){\subseteq}I$. An ideal P of R is a ($\sigma,\;\delta$)-prime ideal of R if P(${\neq}R$) is a ($\sigma,\;\delta$)-ideal and for ($\sigma,\;\delta$)-ideals I and J of R, $IJ{\subseteq}P$ implies that $I{\subseteq}P$ or $J{\subseteq}P$. An ideal Q of R is ($\sigma,\;\delta$)-semiprime ideal of R if Q is a ($\sigma,\;\delta$)-ideal and for ($\sigma,\;\delta$)-ideal I of R, $I^2{\subseteq}Q$ implies that $I{\subseteq}Q$. The ($\sigma,\;\delta$)-prime radical (resp. prime radical) is defined by the intersection of all ($\sigma,\;\delta$)-prime ideals (resp. prime ideals) of R and is denoted by $P_{(\sigma,\delta)}(R)$(resp. P(R)). In this paper, the following results are obtained: (1) $P_{(\sigma,\delta)}(R)$ is the smallest ($\sigma,\;\delta$)-semiprime ideal of R; (2) For every extended endomorphism $\bar{\sigma}$ of $\sigma$, the $\bar{\sigma}$-prime radical of an Ore extension $P(R[x;\sigma,\delta])$ is equal to $P_{\sigma,\delta}(R)[x;\sigma,\delta]$.

• BMB Reports
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• v.55 no.9
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• pp.447-452
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• 2022

Neurogenic differentiation 1 (NeuroD1) is an essential transcription factor for neuronal differentiation, maturation, and survival, and is associated with inflammation in lipopolysaccharide (LPS)-induced glial cells; however, the concrete mechanisms are still ambiguous. Therefore, we investigated whether NeuroD1-targeting miRNAs affect inflammation and neuronal apoptosis, as well as the underlying mechanism. First, we confirmed that miR-30a-5p and miR-153-3p, which target NeuroD1, reduced NeuroD1 expression in microglia and astrocytes. In LPS-induced microglia, miR-30a-5p and miR-153-3p suppressed pro-inflammatory cytokines, reactive oxygen species, the phosphorylation of c-Jun N-terminal kinase, extracellular-signal-regulated kinase (ERK), and p38, and the expression of cyclooxygenase and inducible nitric oxide synthase (iNOS) via the NF-κB pathway. Moreover, miR-30a-5p and miR-153-3p inhibited the expression of NOD-like receptor pyrin domain containing 3 (NLRP3) inflammasomes, NLRP3, cleaved caspase-1, and IL-1β, which are involved in the innate immune response. In LPS-induced astrocytes, miR-30a-5p and miR-153-3p reduced ERK phosphorylation and iNOS expression via the STAT-3 pathway. Notably, miR-30a-5p exerted greater anti-inflammatory effects than miR-153-3p. Together, these results indicate that miR-30a-5p and miR-153-3p inhibit MAPK/NF-κB pathway in microglia as well as ERK/STAT-3 pathway in astrocytes to reduce LPS-induced neuronal apoptosis. This study highlights the importance of NeuroD1 in microglia and astrocytes neuroinflammation and suggests that it can be regulated by miR-30a-5p and miR-153-3p.