• Asian Pacific Journal of Cancer Prevention
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• 제14권12호
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• pp.7517-7522
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• 2013

Background: Cancer incidence rates are increasing particularly in developing countries. It is crucial for policy makers to know basic cancer epidemiology in each region to design comprehensive prevention plans. There have hitherto been no population-based data available for cancer in Khuzestan province. The present report is a first from the regional population-based cancer registry for the period of 2002-2009. Materials and Methods: Data were collected retrospectively reviewing all new cancer patients whom were registered in Khuzestan province cancer registry during an 8-year period (2002-2009). All cases were coded based on the ICD-O-3 coding system and collected data were computerized using SPSS (Chicago, IL) software, version 11.5. The age standardized incidence rates (ASRs) per 100,000 person-year for all cancers were computed using the indirect method of standardization to the world population. Results: During the 8-year study period, 16,801 new cancer cases were registered. Based on the computed ASRs, the five most frequent malignancies in females were breast (26.4 per 100,000), skin (13.6), colorectal (5.72), stomach (4.31) and bladder(4.07) and in males, the five most frequent were skin (16.0 per 100,000), bladder (10.7),prostate (7.64), stomach (7.17), and colorectal (6.32).The ASR for all malignancies in women was 92.5 per 100,000, and that for men was 87.4. Conclusions: The observed patterns from the analysis of Khuzestan cancer registry data will lead to better understanding of the epidemiology of various malignancies in this part ofthe country and consequently provide a useful guide for authorities to make efficacious decisions and policies about a cancer control program for south-west Iran.

• The Journal of Korean Obstetrics and Gynecology
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• 제19권3호
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• pp.191-201
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• 2006

Purpose : Skin Resistance Variability(SRV) is a kind of biofunctional signal, and it can show the function of autonomic nervous system, especially in the sympathetic nerve. The objective of this study is to find out the differences of SRV of reproductive women with aging. Method : We measured SRV(by CP-6000A) of 151 women who visited Gynecologic Clinic. And the results were classified according to age, by five groups. After detection of SRV, we performed correlation analysis and ANOVA by SPSS 12.0. Results : 1. The SRV was measured twice. It resulted in seven areas. In 1, 2, 3 areas, the second results were higher than first results in every groups. In 4, 5, 6, 7 areas, the first results were higher than second results in every groups. 2. The SRV of lower part (4, 5, 6, 7 area) on the body was higher than that of higher part (1, 2, 3 area). 3. The SRV in the youngest group was higher than the oldest group in 1, 2, 3 area of second trial. Conclusion : With relations to the standardization and objectification of oriental medicine, we expect that these results contribute to gynecologic clinic in the department of diagnosis of functional abnormality of hypothalamus-hypophysis-ovarian axis (H-P-O axis).

• YAKHAK HOEJI
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• 제54권6호
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• pp.441-448
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• 2010

In present study, classification and quality control of Genus Polygonatum were developed using the isolated from Polygonati Odorati Rhizoma and Polygonati Rhizoma. 3 components were isolated from Butanol fractions of Polygonati Rhizoma, and 2 components were isolated from Hexane and Butanol fractions of Polygonati Odorati Rhizoma. All the components were obtained using silica gel and ODS column chromatography. The compounds were identified as adenosine, 14-hydroxylfurost-5-ene-3-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}2$)-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}4$)-O-${\beta}$-D-galactopyranosyl-26-O-${\beta}$-D-glucopyranoside, 22-O-methyl-14-hydrocxyfurost-5-ene-3-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}2$)-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}4$)-O-${\beta}$-Dgalactopyranosyl-26-O-${\beta}$-D-glucopyranoside, ${\beta}$-Sitosteryl-3-O-${\beta}$-D-D-glucopyranoside, 14-hydoxylfurost-5-ene-3-O-${\beta}$-Dglucopyranosyl-($1{\rightarrow}2$)-O-[${\beta}$-D-xylopyranosyl-($1{\rightarrow}3$)]-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}4$)-O-${\beta}$-D-galactopyranoside through physicochemical data, spectroscopic methods ($^1H$-NMR, $^{13}C$-NMR, Mass) according references. The quality control of genus Polygonatum were conducted using HPLC quantitative analysis of 14-hydroxylfurost-5-ene-3-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}2$)-O-${\beta}$-D-glucopyranosyl-($1{\beta}4$)-O-${\beta}$-D-galactopyranosyl-26-O-${\beta}$-D-glucopyranoside, 14-hydoxylfurost-5-ene-3-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}2$)-O-[${\beta}$-D-xylopyranosyl-($1{\rightarrow}3$)]-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}4$)-O-${\beta}$-D-galactopyranoside in 30 samples collected throughout Korea and China. This method provided a tool for standardization of mix or misusing the commercial Odorati Rhizoma and Polygonati Rhizoma. As a result, contained quantity of 14-hydroxylfurost-5-ene-3-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}2$)-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}4$)-O-${\beta}$-D-galactopyranosyl-26-O-${\beta}$-D-glucopyranoside was measured $0.008{\pm}0.006%$ and 14-hydoxylfurost-5-ene-3-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}2$)-O-[${\beta}$-D-xylopyranosyl-(13)]-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}4$)-O-${\beta}$-Dgalactopyranoside was measured $0.026{\pm}0.012%$.

• Journal of Physiology & Pathology in Korean Medicine
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• 제23권5호
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• pp.1193-1198
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• 2009

Pulse diagnosis is a central diagnosis method used in traditional Oriental medicine. To standardize and modernize the pulse diagnosis method, it is essential to develop an instrument-based reinterpretation of the clinically used pulse images in terms of the physical quantities such as the strength, period, width, length, and depth of the pulse. As a step towards such standardization, we conducted a clinical study on the floating/sinking pulses based on an automated palpation instrument (3D-MAC, Daeyo Medi, Korea) for 213 female subjects in their 20s and 174 female subjects in their 60s. The floating/sinking pulses are the two representative pulse images depending only on the depth of the pulse, and can be conveniently scaled by the coefficient of the floating-sinking pulse ($C_{fs}{\in}(0,1)$), which represents how strong one should apply the hold-down pressure to obtain the maximal pulse strength. As a result, primarily we found that it tends to appear more floating-like pulse ($C_{fs}{\rightarrow}0$) at Gwan and more sinking-like pulse ($C_{fs}{\rightarrow}1$) at Cheek, at both age groups and at both wrists. This result is consistent with a previous study on the geometrical structure of the blood vessel by an ultrasonograph. Second, the pulse tends to be more sinking-like in the age group of 60s than 20s. Finally, the pulses at the right palpation positions were found to be more sinking-like than the left, at both age groups.

• Korean Journal of Food Science and Technology
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• 제39권4호
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• pp.372-379
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• 2007

A high-performance liquid chromatography-electrospray ionization (HPLC-ESI) tandem MS was developed for the rapid and simultaneous determination of forbidden medicines in dietary supplements. Thirteen medicinal components such as PDE-5 inhibitors and their analogues, and the newly identified dimethylsildenafil and xanthoanthrafil, were included in this study. After tentative standardization of molecular ions in both polarities using thirteen references on the mass spectrometer, with ESI-continuous infusion via the syringe pump method, the relative intensity of the ions present in the resulting spectra was quantitatively compared. From the results, the ion mode was selected depending on each reference's characteristics. A HPLC method coupled with the ESI mode was developed considering the matrix effect and interference depending on the type of sample. The validation test of the developed method was followed by carrying out precision, accuracy, recovery, sensitivity and linearity, etc. The method showed sufficiently high sensitivity, reproducibility, and specificity, and produced 4 times faster results when compared with the existing HPLC/UV method for the determination of forbidden compounds in dietary supplements.

• Korean Journal of Applied Biomechanics
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• 제17권4호
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• pp.201-208
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• 2007

The purpose of this study is to compare 4 different body punch types(type 1: a punch using a shoulder, type 2: a punch using a waist, type 3: a punch using lower extremities, and type 4: a punch with elbows by your side at chest level) in horseback-riding stance and establish suitable teaching theory and method, which would be a useful reference to Taekwondo instructors on the spot(in Taekwondo dojangs all around Korea). Five exhibition players from Korean national Taekwondo exhibition team participated in this study. Each participant was asked to perform the four different types of punches and their kinematic and kinetic data were recorded with 7 vicon cameras(125Hz) and two force plates(AMTI, 1200Hz). We analyzed displacement, time, resultant center of body mass trajectory, velocity, trunk angular velocity, and ground reaction force(GRF) from each body segment in body punch and the result. I performed 1-way ANOVA(RM) for average values of each player after standardization and statistical significance was set as p<.05. was as the following ; First, they showed a tendency to take the body punch posture with the biggest motion at a shoulder and on descending order a waist and a knee. Second, a mean time for each body punch on ascending order 0.46sec. for type 2, 0.49sec for type 3, 0.50sec. for type 4, and 0.56sec. for type 1. Third, a mean resultant center of body mass trajectory for each body punch the longest 4.07cm for type 3 and the shortest 2.458cm for type 1. Fourth, a mean of maximal velocity of a fist strike was the fastest 5.99m/s for type 3, 5.93m/s for type 4, 5.67m/s for type 2, and 5.01m/s for type 1 on the descending order. Fifth, a mean of maximal trunk angular velocity of the fastest 495.6deg./sec. for type 4 and 337.7deg./sec. for type 1 on the descending order. Sixth, strongest value was type 3, 2 for anterior-posterior ground reaction force(left -54.89N, right 60.58N), type 4 for medial-lateral GRF(left 83.59N, right -80.12N), and type 3 for vertical GRF(left 341.79N, right 426.11N).

• Restorative Dentistry and Endodontics
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• 제33권4호
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• pp.332-340
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• 2008

The purpose of this study was to evaluate the viability of periodontal ligament cell in rat teeth using slow cryopreservation method with magnetic field through MTT assay and TUNEL test. For each group, 12 teeth of 4 weeks old white female Sprague-Dawley rat were used for MTT assay, and 6 teeth in TUNEL test. The Maxillary left and right, first and second molars were extracted as atraumatically as possible under tiletamine anesthesia. The experimental groups were group1 (immediately extraction), group 2 (cold preservation at 4$^{\circ}C$ for 1 week), group 3 (rapid cryopreservation in liquid nitrogen), group 4 (slow cryopreservation with magnetic field of 1 G), and group 5 (slow cryopreservation). F medium was used as preservation medium and 10% DMSO as cryoprotectant. After preservation and thawing, the MTT assay and TUNEL test were processed. One way ANOVA and Scheffe method were performed at the 95% level of confidence. The value of optical density obtained after MTT analysis was divided by the value of eosin staining for tissue volume standardization. In both MTT assay and TUNEL test, it had showed no significant difference among group 3, 4, and 5. And group 3 had showed higher viability of periodontal ligament cell than group 2. From this study, slow cryopreservation method with magnetic field can be used as one of cryopreservation methods.

• Asian Pacific Journal of Cancer Prevention
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• 제13권11호
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• pp.5477-5482
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• 2012

Background: The HER-2/neu gene is altered in 15-20% of breast cancer patients. Immunohistochemistry (IHC) is considered to be the most cost-effective method for HER-2 detection in many countries. Approximately 8,000 new cases of breast cancer are observed annually in Iran. The aims of this study were to conduct a systematic review of the literature on the rate of HER-2-positive breast cancer diagnosed by IHC in Iran. Methods: A systematic search of the medical literature using the Medline/PubMed, ISI and SID databases revealed articles published in the English and Persian languages evaluating HER-2-positive breast cancer in Iran. Results: From 22 studies, 3,033 patients were evaluated, of whom 1,350 were diagnosed as HER-2-positive by IHC HER-2 testing. The mean percentage of HER-2-positive patients was 44.5%, which is higher than that recorded in international statistics. Results of this meta-analysis showed a significant heterogeneity between ratios. There was a statistically significant difference between the results of pre- and post implementation of 2007 American Society of Clinical Oncology/College of American Pathologists (ASCO/CAP) guideline. IHC HER-2 testing has been performed in Iran for over 10 years. Similar to many other countries, before establishment of an infrastructure for IHC diagnostic tests, HER-2 testing was routinely performed in Iran. Our study showed that the statistics reported from Iran varied widely; for instance, the rate of HER-2-positive cases varied from 23.3% to 81.0%. Conclusions: Our results demonstrate that the lack of standardization and harmonization of this test have led to marked variations in breast cancer diagnosis in Iran.

• Restorative Dentistry and Endodontics
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• 제31권1호
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• pp.58-65
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• 2006

The possibility of applying a hi-axial flexure strength test on composite resin was examined using three point and hi-axial flexure strength tests to measure the strength of the light-cured resin and to compare the relative reliability using the Weibull modulus. The materials used in this study were light-curing restorative materials, $MICRONEW^{TM},\;RENEW^{(R)}$ (Bisco, Schaumburg, USA). The hi-axial flexure strength measurements used the piston-on-3-ball test according to the regulations of the International Organization for Standardization (ISO) 6872 and were divided into 6 groups, where the radius of the specimens were 12mm (radius connecting the 3-balls: 3.75mm), 16 mm(radius connecting the 3-balls: 5mm), and the thickness were 0.5mm, 1mm, 2mn for each radius. The hi-axial flexure strength of the $MICRONEW^{TM}\;and\;RENEW^{(R)}$ were higher than the three point flexure strength and the Weibull modulus value were also higher in all of the bi-axial flexure strength groups, indicating that the hi-axial strength test is relatively less affected by experimental error. In addition, the 2 mm thick specimens had the highest Weibull modulus values in the hi-axial flexure strength test, and the $MICRONEW^{TM}$ group showed no significant statistical difference (p>0.05). Besides the 2mm $MICRONEW^{TM}$ group, each group showed significant statistical differences (p<0.05) according to the thickness of the specimen and the radius connecting the 3-balls. The results indicate that for the 2mm group, the hi-axial flexure strength test is a more reliable testing method than the three point flexure strength test.

• KOREAN JOURNAL OF CROP SCIENCE
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• 제64권4호
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• pp.452-458
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• 2019

Coix lacryma-jobi var. ma-yuen (Rom. Caill.) Stapf (CL), which contains riboflavin and coixol, has traditionally been used to treat cancer and arthritis. However, no method for the simultaneous determination of riboflavin and coixol in CL sprouts has been established. In this study, we established and validated a high-performance liquid chromatography-diode array detection (HPLC-DAD) method for the identification and quantification of two reference markers, riboflavin and coixol, in CL sprout extracts. CL sprouts (whole sprouts and leaves) were subjected to extraction with 70% ethanol at room temperature and at 80 ℃ under reflux conditions. The two extractions were validated with respect to specificity, accuracy, precision, and linearity. The content of the two reference markers was highest in leaves extracted under reflux conditions (riboflavin, 8.23 ± 0.32 mg/g; coixol, 5.95 ± 0.04 mg/g). We also investigated the antioxidant activity of the extracts via 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS⁺) scavenging assays. The results indicated that extracts obtained from sprouts under reflux conditions had the strongest antioxidative effects (DPPH half maximal inhibitory concentration [IC₅₀], 68.9 ± 4.1 g/mL; and ABTS, IC₅₀, 34.9 ± 0.1 g/mL). These results can serve as baseline data for the simultaneous determination of the two reference marker compounds, riboflavin and coixol, and development of functional food materials using CL sprouts.