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Content Analysis and Classification for Polygonati Odorati Rhizoma and Polygonati Rhizoma by Steroidal Saponin  

Kim, Sun-Gun (College of Pharmacy Chung-Ang University)
Shin, So-Young (College of Pharmacy Chung-Ang University)
Moon, Ye-Ji (College of Pharmacy Chung-Ang University)
Seo, Ji-Yoon (College of Pharmacy Chung-Ang University)
Kim, Ho-Kyoung (Korea Institute of Oriental Medicine)
Whang, Wan-Kyunn (College of Pharmacy Chung-Ang University)
Publication Information
YAKHAK HOEJI / v.54, no.6, 2010 , pp. 441-448 More about this Journal
Abstract
In present study, classification and quality control of Genus Polygonatum were developed using the isolated from Polygonati Odorati Rhizoma and Polygonati Rhizoma. 3 components were isolated from Butanol fractions of Polygonati Rhizoma, and 2 components were isolated from Hexane and Butanol fractions of Polygonati Odorati Rhizoma. All the components were obtained using silica gel and ODS column chromatography. The compounds were identified as adenosine, 14-hydroxylfurost-5-ene-3-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}2$)-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}4$)-O-${\beta}$-D-galactopyranosyl-26-O-${\beta}$-D-glucopyranoside, 22-O-methyl-14-hydrocxyfurost-5-ene-3-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}2$)-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}4$)-O-${\beta}$-Dgalactopyranosyl-26-O-${\beta}$-D-glucopyranoside, ${\beta}$-Sitosteryl-3-O-${\beta}$-D-D-glucopyranoside, 14-hydoxylfurost-5-ene-3-O-${\beta}$-Dglucopyranosyl-($1{\rightarrow}2$)-O-[${\beta}$-D-xylopyranosyl-($1{\rightarrow}3$)]-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}4$)-O-${\beta}$-D-galactopyranoside through physicochemical data, spectroscopic methods ($^1H$-NMR, $^{13}C$-NMR, Mass) according references. The quality control of genus Polygonatum were conducted using HPLC quantitative analysis of 14-hydroxylfurost-5-ene-3-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}2$)-O-${\beta}$-D-glucopyranosyl-($1{\beta}4$)-O-${\beta}$-D-galactopyranosyl-26-O-${\beta}$-D-glucopyranoside, 14-hydoxylfurost-5-ene-3-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}2$)-O-[${\beta}$-D-xylopyranosyl-($1{\rightarrow}3$)]-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}4$)-O-${\beta}$-D-galactopyranoside in 30 samples collected throughout Korea and China. This method provided a tool for standardization of mix or misusing the commercial Odorati Rhizoma and Polygonati Rhizoma. As a result, contained quantity of 14-hydroxylfurost-5-ene-3-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}2$)-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}4$)-O-${\beta}$-D-galactopyranosyl-26-O-${\beta}$-D-glucopyranoside was measured $0.008{\pm}0.006%$ and 14-hydoxylfurost-5-ene-3-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}2$)-O-[${\beta}$-D-xylopyranosyl-(13)]-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}4$)-O-${\beta}$-Dgalactopyranoside was measured $0.026{\pm}0.012%$.
Keywords
Polygonati rhizoma; Polygonati odorati rhizoma; steroidal saponin; origin; content analysis;
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Times Cited By KSCI : 1  (Citation Analysis)
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1 Pao, L. H., Kim, H. G., Ru, R. W. and Chun, N. L. : Benzoquinones, a homoisoflavanone and other constituents from Polygonatum Alte-lobatum. Phytochem. 44, 1369 (1997).   DOI   ScienceOn
2 Chang, I. M., Yun, H. S. and Kazuo, Y. : Revision of $^{13}C$ NMR Assignments of $\beta$-sitosterol and ${\beta}-sitostery-3-O-{\beta}-D-glucopranoside$ Isolated from Plantago asiatica Seed. Kor. J. Pharmacogn. 12, 12 (1981).
3 Wang, D., Li D., Zhu, W., Zhang, J. F. and Peng, P. : Steroidal saponins from the rhizomes of Polygonatum odoratum. Nat. Prod. Res. 23, 940 (2009).   DOI   ScienceOn
4 Lee, M. Y., Kim, K. H., Kim, Y. H., Oh, S. H., Kang, J. K. and Ko, B. S. : A Taxonomic examination of Polygonatum Rhizoma and Polygonati Odorati Rhizoma based on RAPD analysis. Kor. J. Pharmacogn. 35, 265 (2004).   과학기술학회마을
5 Qin, H. L., Li, H., Wang, P. and Si, L. X. : The New secondary metabolite from Polygonatum odoratum. Zhongguo Zhongyao Zazhi 29, 42 (2004)
6 Liu, T., Li, Z. and Liu, C. : Analysis of Polygonatum odoratum and its substitutes by RAPD. Zhongguo Yaozue Zazhi 37, 734 (2002).
7 Jang, C. G. : A taxonomic reviw of Korea Polygonatum (Ruscaceae). Kor. J. Plant Tax. 32, 417 (2002).
8 식품의약품안전청 의약품안전국 의약품평가부 : 의약품 등 분석법의 밸리데이션에 대한 가이드라인, 식품의약품안전청, 서울 p. 18 (2004).
9 Lim, S. J. and Park, H. J. : The effect of Butanol fraction of Polygonatum odoratum with selenium on blood glucose level and lipid peroxidation in streptozotocin-induced diabetic rats. Kor. J. Nutr. 33, 703 (2000).
10 Domondon, D. L., He, W., De Kimpe, N., Hfte, M. and Poppe. J. : $\beta$-Adenosine, a bioactive compound in grass chaff stimulatin mushroom production. Phytochem. 65, 181 (2004).   DOI   ScienceOn
11 李珍 : 本草綱目, 高文社, 417 (1973).
12 Zhang, J., Ma, B. P., Kang, L. P., Yu, H. S., Yang, Y., Yan, X. Z. and Dong, F. T. : Furostanol Saponins from the fresh Rhizomes of Polygonatum kingianum. Chem. Pharm. Bull. 54, 931 (2006).   DOI   ScienceOn
13 Son, K. H., Do, J. C. and Kang, S. S. : Steroidal saponins from the Rhizome of Polygonatum sibiricum. J. Nat. Prod. 53, 333 (1990).   DOI
14 唐徽 : 經史證類大觀本草, 崇文社 (1976).
15 許浚 : 東醫報鑑, 湯液篇, 南山堂, 719 (1974).
16 鄕藥集成方, 卷七十八 草部上品卷上 (1943).
17 Ahn, D. G. : Restorative of Korea. Yeollin Chakdle, Seoul, Korea, p. 307 (1993).
18 일본약국방 제14개정판 : 광천서점, 동경 (2001).
19 식품의약품안전청 : 대한약전외 한약(생약) 규격집, 서울 (2007).
20 Chinese Pharmacopoeia Commission : PHARMACOPOEIA OF THE PEOPLE'S REPUBLIC OF CHINA (I), p. 334 (2005).
21 한대석, 한덕룡, 유승조, 백완숙 : 한국.중국.일본의 생약비교연구, 한국의약품수출입협회, 영림사, 서울, p. 142 (1996).
22 1) 陶弘景 : 名醫別錄, 人民生出版社, 北京, 18 (1986).