• The Korean Journal of Mycology
• /
• v.45 no.3
• /
• pp.196-211
• /
• 2017

Despite the significance of external environmental factors in differentiation, putative factors involved in differentiation of Aspergillus nidulans have not yet been fully understood. A sporulation-specific proteome analysis of A. nidulans in the present study revealed that the expression levels of more than 2,400 proteins were affected under conditions inducing sporulation (0.6 M KCl) compared with normal conditions. Among the proteins with predicted functions, two targets, AN1342 and AN9419, were functionally analyzed using targeted deletion strains and phenotypic observations. For AN1342, because the deletion of the corresponding open reading frame caused a reduction in stalk length during asexual development and in pigment production in liquid culture, the gene was designated as sspA ($\underline{s}hort$ $\underline{s}talk$ & $\underline{p}igment$). Deletion of the AN9419 gene, which is predicted to encode alanyl-tRNA synthetase, led to severe growth defects due to alanine auxotrophy and abolishment of asexual reproduction and thus, the gene was designated as alaA.

• Korean journal of applied entomology
• /
• v.35 no.1
• /
• pp.38-44
• /
• 1996

These experiments were conducted to investigate the ecological characteristics of Amblyseius womersleyi Schica,which was biological agent in the integrated management of tea red spider mite, Tetranychus kanzawai Kishida.At four constant temperatures of 15, 20, 25, and 30$^{\circ}$C, the periods of A. womersleyi from egg to adult emergencewere 22.8, 9.6, 4.8, and 3.6 days, respectively. Developmental threshold and effectively cumulative degree-day from egg to adult emergence were 12.7"C and 61.4DD, respectively. Preoviposition periods of A.womersleyi were shorter than oviposition and postoviposition periods. The number of eggs laid per female was21.7, 28.1, 34.2, and 48.0 at the respective temperatures. The intrinsic rates of increase were estimated to be 0.064, 0.139, 0.349, and 0.402; the mean generation times were 36.0, 19.3, 8.6, and 7.7 days; the populationdoubling times were 10.8, 5.0, 2.0, and 1.7 days, respectively. A. womersleyi overwintered on green weeds, fallenleaves and in the soil surface as adult females. A. womersleyi gravid female consumed 3.0, 4.9, 15.7, and19.4 eggs of T. kanzawai per day at 15, 20, 25, and 30$^{\circ}$C, respectively. A. womersleyi protonymph consumed4.6 eggs, deutonymph 6.3 eggs, and female during oviposition consumed 19.4 eggs, and 6.8 eggs of T. kanzawaiduring postoviposition per day at 30$^{\circ}$C. at 30$^{\circ}$C.

• Nutrition Research and Practice
• /
• v.13 no.1
• /
• pp.58-63
• /
• 2019

BACKGROUND/OBJECTIVES: Telomeres are located at the chromosomal ends and progressively shortened during each cell cycle. Telomerase, which is regulated by hTERT and c-MYC, maintains telomeric DNA sequences. Especially, telomerase is active in cancer and stem cells to maintain telomere length for replicative immortality. Recently we reported that walnut phenolic extract (WPE) can reduce cell viability in a colon cancer stem cell (CSC) model. We, therefore, investigated the effect of WPE on telomere maintenance in the same model. MATERIALS AND METHODS: $CD133^+CD44^+$ cells from HCT116, a human colon cancer cell line, were sorted by Fluorescence-activated cell sorting (FACS) and treated with WPE at the concentrations of 0, 10, 20, and $40{\mu}g/mL$ for 6 days. Telomere lengths were assessed by quantitative real-time PCR (qRT-PCR) using telomere specific primers and DNA extracted from the cells, which was further adjusted with single-copy gene and reference DNA ($ddC_t$). Telomerase activity was also measured by qRT-PCR after incubating the PCR mixture with cell protein extracts, which was adjusted with reference DNA ($dC_t$). Transcriptions of hTERT and c-MYC were determined using conventional RT-PCR. RESULTS: Telomere length of WPE-treated cells was significantly decreased in a dose-dependent manner ($5.16{\pm}0.13$ at $0{\mu}g/mL$, $4.79{\pm}0.12$ at $10{\mu}g/mL$, $3.24{\pm}0.08$ at $20{\mu}g/mL$ and $3.99{\pm}0.09$ at $40{\mu}g/mL$; P = 0.0276). Telomerase activities concurrently decreased with telomere length ($1.47{\pm}0.04$, $1.09{\pm}0.01$, $0.76{\pm}0.08$, and $0.88{\pm}0.06$; P = 0.0067). There was a positive correlation between telomere length and telomerase activity (r = 0.9090; P < 0.0001). Transcriptions of both hTERT and c-MYC were also significantly decreased in the same manner. CONCLUSION: In the present cell culture model, WPE reduced telomere maintenance, which may provide a mechanistic link to the effect of walnuts on the viability of colon CSCs.

• Journal of Oral Medicine and Pain
• /
• v.36 no.1
• /
• pp.39-51
• /
• 2011

The aims of this study were to investigate whether the facial skeletal patterns previously reported to be related to temporomandibular disorder (TMD) in other studies could be consistently observed in the TMD patients diagnosed according to Research Diagnostic Criteria for Temporomandibular Disorder (RDC/TMD) Axis I and evaluate its usability in the orthodontic clinics to examine the patients with TMD related symptoms. The clinical records and radiographs of female patients who visited the TMD and Orofacial Pain Clinic of Seoul National University Dental Hospital and were diagnosed as TMD were consecutively filed for this study. Patients were clinically examined and diagnosed according to the revised diagnostic algorithms of RDC/TMD Axis I and the lateral cephalogram, panoramic orthopantomogram, temporomandibular joint (TMJ) orthopantomogram, and transcranial radiograph of each patient were taken and digitalized. The data of patients who were under 18 years of age or had any systemic disease, trauma history involving the TMJ, or skeletal deformity at the time of the first examination were excluded. The remaining data of 96 female patients were finally analyzed. The obtained results were as follows: 1. There are no significant differences of cephalometric measurements between RDC I (muscle disorders) diagnostic groups. 2. Only the articular angle of the RDC group IIc (disk displacement without reduction without limited opening) patients was larger than patients of the no diagnosis of RDC II group (disk displacement). 3. Larger articular angle and smaller facial height ratio were observed in RDC IIIc group (osteoarthrosis) compared to IIIa group (arthralgia). Larger articular angle, larger Bjork sum, smaller posterior facial height, and smaller facial height ratio were observed in RDC group IIIc compared to no diagnosis of RDC III group (arthralgia, arthritis, and arthrosis). 4. According to the results of cephalometric analysis in simplified RDC groups, smaller overjet was observed in muscle disorders (MD) group. Facial height ratio and IMPA were smaller and articular angle was larger in disk displacements (DD) group than in no diagnosis of DD group. In arthrosis (AR) group, posterior facial height, and facial height ratio were smaller, and articular angle, gonial angle, facial convexity, FMA, Bjork sum, and ANB were larger than in no diagnosis of AR group. In joint pain (JP) group, only posterior facial height was smaller than no diagnosis of JP group. In conclusion, Facial morphologic patterns showing posterior-rotated mandible and lower posterior facial height is related to RDC group II and III diagnosis of the TMJ in female TMD patients. RDC/TMD Axis I diagnosis can provide a good clinical diagnostic tool for the standardized examination of the TMJ in orthodontic clinics.

• Korean Journal of Environmental Biology
• /
• v.37 no.4
• /
• pp.568-578
• /
• 2019

The nymphal development of the potato aphid, Macrosiphum euphorbiae (Thomas), was studied at seven constant temperatures (12.5, 15.0, 17.5, 20.0, 22.5, 25.0, and 27.5±1℃), 65±5% relative humidity (RH), and 16:8 h light/dark photoperiods. The developmental investigation of M. euphorbiae was separated into two steps, the 1^st through 2^nd and the 3^rd through 4^th stages. The mortality was under 10% at six temperatures. However, it was 53.0% at 27.5℃. The developmental time of the entire nymph stage was 15.5 days at 15.0℃, 6.7 days at 25.0℃, and 9.7 days at 27.5℃. In the immature stage, the lower threshold temperature of the larvae was 2.6℃ and the thermal constant was 144.5 DD. In our analysis of the temperature-development experiment, the Logan-6 model equation was most appropriate for the non-linear regression models (r²=0.99). When the distribution completion model of each development stage of M. euphorbiae larvae was applied to the 2-parameter and 3-parameter Weibull functions, each of the model's goodness of fit was very similar (r²=0.92 and 0.93, respectively). The adult longevity decreased as the temperature increased but the total fecundity of the females at each temperature was highest at 20℃. The life table parameters were calculated using the whole lifespan periods of M. euphorbiae at the above six temperatures. The net reproduction rate (R₀) was highest at 20.0℃(63.2). The intrinsic rate of increase (r_m) was highest at 25℃(1.393). The finite rate of doubling time (D_t) was the shortest at 25.0℃(2.091). The finite rate of increase (λ) was also the highest at 25.0℃(1.393). The mean generation time(T) was the shortest at 25.0℃(9.929).

• Korean journal of applied entomology
• /
• v.52 no.4
• /
• pp.387-394
• /
• 2013

The developmental times of mealworm beetle larvae, Tenebrio molitor were studied at six temperatures ranging from 15 to $30^{\circ}C$ with 60~70% RH, and a photoperiod of 14L:10D. Mortality of larval period was very low at 17 and $20^{\circ}C$ but did not die over $22^{\circ}C$. Developmental time of larva was decreased with increasing temperature. The total developmental time of T. molitor larvae was longest at $17^{\circ}C$ (244.3 days) and shortest at $30^{\circ}C$ (110.8 days). Egg and larvae were not developed at $15^{\circ}C$. The lower developmental threshold and effective accumulative temperatures for the total larval stages were $6.0^{\circ}C$ and 2564.1 degree-days, respectively. The relationship between developmental rate and temperature was fitted by a linear model and nonlinear model of Logan-6($r^2$=0.95). The distribution of completion of each development stage was well described by the 2-parameter Weibull function ($r^2$=0.8502~0.9390).

• Korean Journal of Plant Resources
• /
• v.33 no.4
• /
• pp.311-336
• /
• 2020

The flora of Sejong were summarized as 531 taxa including 104 families, 315 genera, 462 species, 3 subspecies, 59 varieties and 7 form. The rare plants were 8 taxa including Nepeta cataria L. and Aristolochia ontorta Bunge. The Korea endemic plants were 9 taxa including Populus tomentiglandulosa T. B. Le and Clematis trichotoma Nakai.. The specific plants were 42 taxa including Phytolacca esculenta Van Houtte(V), Corydalis decumbens (Thunb.) Pers(IV), Poncirus trifoliata Raf(III), Pseudostellaria coreana (Nakai) Ohwi(II), Cyrtomium fortunei J.Sm.(I). The naturalized plants were 49 taxa including Sicyos angulatus L, Ambrosia trifida L, Physalis angulata L. Panicum dichotomiflorum Michx., Galinsoga parviflora Cav, and Erigeron philadelphicus L. Especially, Sicyos angulatus L., Phytolacca americana L. and Bidens frondosa L. have been spread throughout the country are required to manage. The naturalized index(NI) and urbanized index(UI) were 8.7% and 15.3%. Useful plants incuding Edible was 199 taxa, Pasture was 193 taxa, Medicinal was 178 taxa, Ornamental was 82 taxa, Timber was 25 taxa, Fiber was 18 taxa, Stain was 9 taxa. Useful plants will be important materials for plant design in recreational and healing forests.

• Korean Journal of Social Welfare
• /
• v.67 no.4
• /
• pp.279-299
• /
• 2015

This study examines the impact of Long-Term Care Insurance(LTCI) on family caregivers(especially focused on female household members) labor supply in South Korea. When public care and informal care are substitutes, LTCI will change allocation of time of family caregivers to spend more time to paid work. The impact of LTCI on labor supply depends on each country's institutional level of public care services. If public care can not substitute for informal care, labor supply of family caregivers will not rise significantly. The conclusions of vigorous empirical study from western countries' are incompatible and problem of endogeneity in terms of methodology has been raised consistently. The dataset of this study are used the third and ninth waves of Korea Welfare Panel. As a result, the introduction of LTCI had no effect on labor supply of household members. Robust findings suggest the positive effects of caregiving on labor market outcomes in simple comparison t-test, but not in fixed-effect regression. Compared with western countries, South Korea's public care services can be interpreted as a supplement to only part that remained at the level does not substitute informal care. These findings may suggest that if LTCI become much more prevalent in the future, senior citizens and family members will be able to choose the LTCI arrangement that best suits their needs.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.32 no.1
• /
• pp.75-88
• /
• 2005

Ameloblastoma is the most commonly occurring odontogenic tumor in oral cavity. Although most are benign epithelial neoplasm, they are generally considered to be locally aggressive and destructive, exhibiting a high rate of recurrence. The biological behavior of this neoplasm is a slowly growing, locally invasive tumor without metastasis, therefore malignant neoplasm, changed its histological appearance to carcinoma or showed distant metastasis, is only defined clinically. In this study, we identified the differentially expressed genes(DEGs) in stages under benign or malignant ameloblastoma compared with normal patient using ordered differential display(ODD) reverse transcription(RT)-PCR and $GeneFishing^{TM}$ technology. ODD RT-PCR is rather effective when the investigation of samples containing very small amounts of total RNA must be accomplished. ODD RT-PCR used the means of amplification with anchored T-primer and adaptor specific primer. bearing definite two bases at their 3' ends and so this method could display differential 3'-expressed sequence taqs(ESTs) patterns without using full-length cDNAs. Compared with standard differential display, ODD RT-PCR is more simple and have enough sensitivity to search for molecular markers by comparing gene expression profiles, However, this method required much effort and skill to perform. $GeneFishing^{TM}$ modified from DD-PCR is an improved method for detecting differentially expressed genes in two or more related samples. This two step RT-PCR method uses a constant reverse primer(anchor ACP-T) to prime the RT reaction and arbitrary primer pairs(annealing control primers, ACPs) during PCR. Because of high annealing specificity of ACPs than ODD RT-PCR, the application of $GeneFishing^{TM}$ to DEG discovery generates reproducible, authentic, and long(100bp to 2kb) PCR products that are detectable on agarose gels. Consequently, various DEGs observed differential expression levels on agarose gels were isolated from normal, benign, and malignant tissues using these methods. The expression patterns of the some isolated DEGs through ODD RT-PCR and $GeneFishing^{TM}$ were confirmed by Northern blot analysis and RT-PCR. The results showed that these identified DEGs were implicated in ameloblastoma neoplasm processes. Therefore, the identified DEGs will be further studied in order to be applied in candidate selection for marker as an early diagnosis during ameloblastoma neoplasm processes.

• Korean journal of applied entomology
• /
• v.53 no.4
• /
• pp.449-456
• /
• 2014

Life table analysis and temperature-dependent development experiments were conducted to understand the biological characteristics of the cabbage aphid, Brevicoryne brassicae (Linnaeus) on detached Tah Tsai Chinese cabbage (Brassica campestris var. narinosa) leaves at seven constant temperatures (15, 18, 21, 24, 27, 30 and $33{\pm}1^{\circ}C$; $65{\pm}5%$ RH; 16L:8D). Mortality was lowest at $24^{\circ}C$ with 18% and 0% at $1^{st}{\sim}2^{nd}$ and $3^{rd}{\sim}4^{th}$ nymphal stages, respectively. The developmental period of $1^{st}{\sim}2^{nd}$ nymphal stage was 8.4 days at $18^{\circ}C$, and it decreased with increasing temperature. The developmental period of the $3^{rd}{\sim}4^{th}$ nymphal stage was 6.7 days at $18^{\circ}C$. The lower threshold temperature calculated using a linear model was $7.8^{\circ}C$, and the effective accumulative temperature was 120.1DD. Adult longevity was 14.9 days at $21^{\circ}C$, and total fecundity was observed 58.5 at $24^{\circ}C$. According to the life table, the net reproduction rate was 47.5 at $24^{\circ}C$, and the intrinsic rate of increase and the finite rate of increase were 0.36 and 1.43, respectively, at $27^{\circ}C$. The doubling time was 1.95d at $27^{\circ}C$, and mean generation time was 7.43d at $30^{\circ}C$.