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Analysis of the Stoichiometry and the Domain for Interaction of Simian Virus 40 Small-t Antigen with Protein Phosphatase 2A

  • Yang, Sung-Il;Mumby, Marc C.
    • BMB Reports
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    • v.28 no.4
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    • pp.331-335
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    • 1995
  • Simian virus 40 (SV40) small-t antigen (small-t) has been known to regulate the activity of a cellular enzyme, protein phosphatase 2A (PP2A), composed of A. B, and C subunits, via binding to the A subunit In the study presented here, the stoichiometry of the binding of small-t to PP2A was determined to be 1: 1. It was also shown that small-t binds to the AC form of PP2A with a higher apparent affinity than it binds to the free A subunit. We also characterized the interaction of PP2A with wild-type and various mutant small-ts. A single-point mutant (Val134Met) and a double-point mutant (Trp147Gly;Leu152 Pro) of small-t exhibited 3-fold and 5-fold lower potencies in inhibiting PP2A activity. respectively. This suggests that the region around amino acids between 134 and 152 of small-t might be important in regulating the enzyme activity of PP2A.

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SOME APPLICATION OF THE UNION OF TWO 𝕜-CONFIGURATIONS IN ℙ2

  • Shin, Yong-Su
    • Journal of the Chungcheong Mathematical Society
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    • v.27 no.3
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    • pp.413-418
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    • 2014
  • It has been proved that the union of two linear star-configurations in $\mathbb{P}^2$ of type s and t for either $3{\leq}t{\leq}10$ or $\(\frac{t}{2}\)-1{\leq}s$ with $3{\leq}t$ has maximal Hilbert function. We extend the condition to $\[\frac{1}{2}\(\frac{t}{2}\)\]{\leq}s$, so that it is true for either $3{\leq}t{\leq}10$ or $\[\frac{1}{2}\(\frac{t}{2}\)\]{\leq}s$ with $3{\leq}t$, which extends the result of [6].

Effects of Mugwort Powder Supplementation on Carcass and Meat Characteristics in the Finishing Period of Gilts (인진쑥 첨가가 비육기 암퇘지의 도체 및 육질 특성에 미치는 영향)

  • Kim, Il-Suk;Jin, Sang-Keun;Kang, Suk-Nam
    • Food Science of Animal Resources
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    • v.29 no.2
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    • pp.188-193
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    • 2009
  • This study was conducted to determine the effect of mugwort powder on meat composition and sensory characteristics in finishing gilts $(LY{\times}D)$. Twenty pigs (75kg) were randomly allotted to four treatments. Mugwort powder was added to final concentrations of 0(C), 0.5(T1), 1.0(T2), and 1.5%(T3) in the basal diet. Pigs were fed with the specified diet for 50 days. Carcass characteristics and cooking loss were not significantly affected by mugwort powder supplementation. The WHC and cooking loss of T2 and T3 were not significantly different than the control. However, the pH values for meat from gilts fed the T1 and T3 diets were significantly lower than the control (p<0.05). Shear force values of fresh meat were highest with the T3 diet (p<0.05), while the shear force values of cooked meat were significantly lower than the control with the T2 and T3 diets. Texture values of hardness, brittleness, gumminess, chewiness, and adhesiveness were significantly higher with the T3 diet (p<0.05), however values for cohesiveness and springiness were not significantly affected by mugwort powder supplementation. These results indicate that the feeding of mugwort powder improves the shear force of cooked meat from gilts fed the T3 diet, however the carcass, WHC and cooking loss characteristics in gilts are not affected by mugwort powder supplementation.

The Differences of Self Efficacy, Self-esteem and Vitality according to the Physical Exercise, Thinking about Health of Nursing Students (간호학생의 운동수행여부, 건강에 대한 생각에 따른 자기효능감, 자아존중감 및 활력정도의 차이)

  • Jeong, Gye-Seon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.4
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    • pp.117-125
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    • 2020
  • This study was conducted to investigate differences in self-efficacy, self-esteem, and vitality related to physical exercise and health consciousness. A descriptive research design, x2 test, and t test were used. Data were collected from May 1 to June 30, 2019, at K city. Participants were 220 nursing students of mean age 23.7 years; 187 (85.5%) were women, 99 (45.0%) exercised regularly; 138 (62.7%) were positive about their health Ed. Note: Please confirm that these edits convey your intended meaning. The scores Ed. Note: This is not clear. Here, do you mean "mean scores of all participants"? of self-efficacy of health and body were 2.93±0.35 and 3.16±0.37, respectively; self-esteem, 2.94±0.43; and vitality, 3.78±0.52. There were significant differences in self-efficacy of health (t=3.68, p=<.001), motor function (t=5.39, p<.001), psychological function (t=2.10, p=.037), nutritional function (t=2.50, p=.013), efficacy of health management (t=2.37, p=.019), and vitality (t=12.63, p=<.001) related to physical exercise. Self-efficacy of health (t=3.73, p=<.001), motor function (t=3.29, p=.001), psychological function (t=4.01, p<.001), nutritional function (t=2.62, p=.009), efficacy of health management (t=2.51, p=.013), self-efficacy of body (t=2.59, p=.010), self-esteem (t=3.91, p=<.001), and vitality (t=2.92, p=.004) were related to health consciousness. Physical exercise and previous experience of body weight loss contributed to enhanced self-efficacy of health and vitality and health consciousness contributed to enhanced self-efficacy of health and body, self-esteem, and vitality.

Effects of Total Mixed Rations with Corn on Growth and Meat Quality of Castrated Korean Black Goats (옥수수 첨가 TMR 사료 급여가 거세흑염소의 발육과 육질에 미치는 영향)

  • Kim, Sang-Uk;Choi, Young-Sun;Yoo, Dae-Joong;Ku, Min-Jung;Lee, Gi-Ho;Park, Sang-Guk
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.36 no.4
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    • pp.350-356
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    • 2016
  • The objective of this study was to evaluate the effect of different dietary corn levels in Total Mixed Rations (TMR) on growth and meat quality of castrated Korean black goats. For feeding trials, thirty-two castrated Korean black goats with similar age (average of 3 months) and body weight (average of 15.9 kg) were divided into the following four treatment groups: commercial feed (control), TMR containing 15% of corn (T1), TMR containing 25% of corn (T2), and TMR containing 35% of corn (T3) in a randomized complete block design. Final body weight was the highest in T2 (58.9 kg), followed by that in control group (53.1 kg). Average daily gain (ADG) and total dry matter intake (TDMI) were also the highest in T2 (100.2 g and 1,346 g, respectively). However, feed conversion ratio (13.4) was the lowest in T2. Carcass weight and dressing percentage in T2 and T3 were higher than those in the control. For meat quality characteristics, crude fat content was the highest in T2 (6.54%). Cooking loss (15.2%) was the lowest in the control. However, the control had the highest flavor value (5.3%). Oleic acid (C18:l) and palmitic acid (C16:0) were the highest in T2 (48.6% and 22.4%, respectively). Stearic acid (C18:0) was the highest in T1 (14.5%). Unsaturated fatty acid was the highest in the control (58.8%), followed by that in T3 (58.2%), T2 (57.8%), and T1 (54.4%). These results indicate that TMR containing 25% of corn could improve the growth performance and meat quality of castrated Korean black goats.

The Effect of Elastic Band Exercise Training and Detraining on Body Composition and Fitness in the Elder (탄력밴드 운동이 노인의 신체조성과 체력에 미치는 지속적 효과)

  • So, Wi-Young;Song, Misoon;Cho, Be-Long;Park, Yeon-Hwan;Kim, Yeon-Soo;Lim, Jae-Young;Kim, Seon-Ho;Song, Wook
    • 한국노년학
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    • v.29 no.4
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    • pp.1247-1259
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    • 2009
  • Muscle mass is reduced by aging. There seems to be no direct relationship between sarcopenia(muscle loss) and medical cost in the elderly, but lowering muscle mass results in increase risk of fall and decrease of strength, fitness, physical activity, and independent life. This is coupled with physical trouble and chronic degenerative disease such as diabetes, obesity, hyperlipidemia, and hypertension. Thus, sarcopenia is potential risk factor increasing mortality. The purpose of this study was to investigate the effects of elastic band exercise and detraining on sarcopenia prevention related variables, body composition and fitness. The subject of this study was 60~70 aged 14 seniors who participated in exercise program in J-welfare senior center at J-gu in S-city. Elastic band exercise was performed twice per week for 12 weeks. The body composition and fitness variables were measured before 12 weeks of control, after control(before exercise), after 12 weeks of exercise(before detraining), and after 12 weeks of detraining. There was no significant difference in body composition and fitness variables before and after 12 weeks of control, but elastic band exercise before and after 12 weeks has effect on body composition variables such as weight (t=2.978, p=0.001), body mass index (t=3.502, p=0.004), percent body fat (t=2.216, p=0.045), muscle mass (t=-3.837, p=0.002), visceral fat area (t=5.186, p<0.001), and waist-hip ratio (t=3.045, p=0.009) and on fitness variables such as 2-minutes step (t=-6.891 p<0.001), arm curl (t=-4.702, p<0.001), chair stand (t=-4.860, p<0.001), chair sit and reach (t=-5.910, p<0.001), back scratch (t=-3.835, p=0.002), and 8-ft up and go (t=7.560, p<0.001). This exercise effect was continued after 12 weeks of detraining on body composition variables such as weight (t=2.323, p=0.037), body mass index (t=2.503, p=0.026), muscle mass (t=-3.137, p=0.008) and on fitness variables such as 2-minutes step (t=-6.489 p<0.001), chair stand (t=-4.694, p<0.001), chair sit and reach (t=-3.690, p=0.003), and 8-ft up and go (t=7.539, p<0.001). It was found that the elastic band exercise has positive effect on body composition and fitness in the elderly and the effect was maintained over 12 weeks of detraining.

Effects of Urea and Ammonium Sulfate Application on Yield and Nutritive Value of Whole Crop Barley in Reclaimed Tideland (간척지에서 요소 및 유안비료 시용이 총체보리의 생산성과 사료가치에 미치는 영향)

  • Shin Jae-Soon;Kim Won-Ho;Lee Seung-Heon;Lim Young-Chul
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.26 no.1
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    • pp.25-30
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    • 2006
  • This experiment was conducted to compare the fresh yield and dry matter yield, and nutritive values of whole crop barley treated with Urea (200 kg/ha, T2) and ammonium sulfate fertilizer as 200 kg/ha (T3), 300 kg/ha (T4) and 400 kg/ha (T5) at the Bae-Ho reclaimed tideland. Korea from 2003 to 2005. Salt contents of soil in the ammonium sulfate plots (T3, T4, T5) were lower than those of T2. The fresh yields of ammonium sulfate plots (T3, T4, T5) were higher than those of T2 as 62% (p>0.05), 41% (p>0.05) and 23% (p<0.05), respectively. The dry matter yield of T3, T4 and T5 (ammonium sulfate) was significantly (p<0.05) higher at 5,080 kg/ha, 4,667 ka/ha, 4,040 kg/ha, respectively. The total digestible nutrients (TDN) yield tends to have a similar result. Crude protein (CP) content was highest in T3 and CP trends to decrease as the level of ammonium sulfate was increased. Total digestible nutrient (TDN) were high in T3 and T4. The sodium content of T3 and T4 were lower than T2. Based on the study, it was more desirable to use ammonium sulfate (200 kg N/ha) rather than Urea as fertilizer on reclaimed land in terms of forage production and nutritive value.

ASYMPTOTIC PROPERTY OF PERTURBED NONLINEAR SYSTEMS

  • Im, Dong Man;Choi, Sang Il;Goo, Yoon Hoe
    • Journal of the Chungcheong Mathematical Society
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    • v.30 no.1
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    • pp.103-116
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    • 2017
  • In this paper, we show that the solutions to perturbed differential system $$y^{\prime}=f(t,y)+{{\displaystyle\smashmargin{2}{\int\nolimits_{t_0}}^{t}}g(s,y(s),T_1y(s))ds+h(t,y(t),T_2y(t))$$ have asymptotic property by imposing conditions on the perturbed part ${\int_{t_0}^{t}}g(s,y(s),T_1y(s))ds,h(t,y(t),T_2y(t))$, and on the fundamental matrix of the unperturbed system y' = f(t, y).

Age and Growth of Dictyosoma burgeri(VAN DER HOEVEN) (그물베도라치(Dictyosoma burgeri)의 연령과 성장)

  • Kang, Yong-Joo;Kim, Yeong-Hye;Kim, Won-Tae
    • Korean Journal of Ichthyology
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    • v.7 no.2
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    • pp.171-176
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    • 1995
  • Age and growth of Dictyosoma burgeri, sampled in the costal waters off Sanyang, Tongyeong, Ky$\breve{o}$ngnam during December 1992∼November 1993, were studied by otolith reading. Examination of outer margins of the otolith showed that the opaque zone as annulus was formed once a year with being mainly configured in November. Spawning period was estimated from January to March, with a peak in Feburary. Growth of the fish was expressed by the von Bertalanffy's equation as $L_t=23.5977(1-e^{-0.2542(t+2.1302)})$, $W_t=64.5892(1-e^{-0.2542(t+2.1302)})^{2.3002}$, where $L_t$ is total length in ㎝, $W_t$ is total weight in g and t is age.

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Chitinimonas naiadis sp. nov., Isolated from a Freshwater River

  • Padakandla, Shalem Raj;Chae, Jong-Chan
    • Journal of Microbiology and Biotechnology
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    • v.27 no.7
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    • pp.1300-1305
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    • 2017
  • A rod shaped, aerobic, Gram-stain-negative, and motile bacterium, strain $AR2^T$, was isolated from a water sample of Yeongsan river, Republic of Korea. Strain $AR2^T$ clustered closely with the members of the genus Chitinimonas and showed the highest 16S rRNA gene sequence similarity with Chitinimonas prasina $LY03^T$ (96.4%), Chitinimonas viridis $HMD2169^T$ (96.4%), Chitinimonas taiwanensis $cf^T$ (96.2%), and Chitinimonas koreensis $R2A43-10^T$ (94.2%). The predominant fatty acids of strain $AR2^T$ were identified to be summed feature 3 (comprising $C_{16:1}{\omega}7c$ and/or $C_{16:1}{\omega}6c$), $C_{16:0}$, and $C_{10:0}3-OH$. Diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine were found to be the major polar lipids. The genomic DNA G+C content was 60.4 mol%. Based on the polyphasic characterization, the isolated strain $AR2^T$ is described as a representative of a novel species in the genus Chitinimonas, for which the name Chitinimonas naiadis sp. nov. (type strain =$AR2^T$ =KCTC $42755^T$ =JCM $31504^T$) is proposed.