• Bulletin of the Korean Chemical Society
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• v.33 no.1
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• pp.171-176
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• 2012

A novel chemiluminescence (CL) system was established for the determination of cytosine arabinoside (Ara-C) in pharmaceutical preparations. It was showed that a clear CL signal was observed when Eosin Y mixed with Fenton reagent. The CL intensity was decreased significantly when Ara-C was added to the reaction system and partially scavenged the hydroxyl radicals in the solution. The extent of decrease in the CL intensity had a good stoichiometrical relationship with the Ara-C concentration. Based on this, we developed a new method for the determination of Ara-C using a flow injection analysis (FIA) technique with CL detection. Under the optimal conditions, the linear range of Ara-C concentration was $6.0{\times}10^{-9}\sim1.0{\times}10^{-7}mol/L$ (R = 0.9982) with a detection limit of $7.6{\times}10^{-10}mol/L$ (S/N=3), the RSD was 5.6% for $6.0{\times}10^{-8}mol/L$ Ara-C (n = 11). The method was successfully applied to the determination of Ara-C in injection samples. The possible chemiluminescence reaction mechanism was discussed.

• Journal of the Korean Society for Precision Engineering
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• v.26 no.8
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• pp.79-88
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• 2009

This paper presents the analytical stiffness analysis method for a low-DOF planar parallel manipulator. An n-DOF (n<3) planar parallel manipulator to which 1- or 2-DOF serial mechanism is connected in series may be used as a positioning device in planar tasks requring high payload and high speed. Differently from a 3-DOF planar parallel manipulator, an n-DOF planar parallel counterpart may be subject to constraint forces as well as actuation forces. Using the theory of reciprocal screws, the planar stiffness is modeled such that the moving platform is supported by three springs related to the reciprocal screws of actuations (n) and constraints (3-n). Then, the spring constants can be precisely determined by modeling the compliances of joints and links in serial chains. Finally, the stiffness of two kinds of 2-DOF planar parallel manipulators with simple and complex springs is analyzed. In order to show the effectiveness of the suggested method, the results of analytical stiffness analysis are compared to those of numerical stiffness analysis by using ADAMS.

• Journal of Biosystems Engineering
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• v.30 no.5 s.112
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• pp.280-284
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• 2005

Front the production on the farm to the consumer, agricultural products are subject to various physical treatments involving mechanical techniques and devices. It is essential to understand the physical laws governing the response of these biological materials so that the machines, processes, and handling operations can be designed fur maximum efficiency and the highest quality of the end products. A compression test system was developed to test the physical properties of fruits including apple, pear, and peach which may lead to a better understanding of the physical laws. The test system consisted of a digital storage oscilloscope and simple mechanism which can apply quasi-static compression to fresh fruits. Rupture force, energy, and deformation were measured at the five levels of compression speed from 1.25 to 62.5 mm/min for each internal and external tissues. Rupture forces for apple and pear were in the range of 42.2 to 46.2 N and 38.8 to 41.2 N for external and infernal tissues, respectively. Rupture forces fir peach external tissues were in the range of 48.2 to 54.0 N.

• Journal of the Korean Chemical Society
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• v.24 no.4
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• pp.322-328
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• 1980

1,3,5,7-Tetranitro-1,3,5,7-tetraazacyclooctane (homocyclonite) is prepared simultaneously with 1,3,5-trinitro-1,3,5-triazacyclohexane (cyclonite) by nitrolysis of hexamethylenetetramine. The purpose of our study was to detect the existence of intermediate, 1,5-endomethylene-3,7-dinitro-1,3,5,7-tetraazacyclooctane (DPT), by liquid chromatography based on the reaction mechanism, to derive the theoretical equation which is determinated by the ratio of total methylene group vs. amino N, for the proper quantity of paraformaldehyde added in the reaction and to obtain the optimum condition for maximum yield and purity by comparing the experimental results with the theoretical equation.

• Bulletin of the Korean Chemical Society
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• v.34 no.7
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• pp.2023-2028
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• 2013

A kinetic study is reported for the nucleophilic substitution reactions of phenyl Y-substituted-phenyl carbonates (5a-5k) with piperidine in 80 mol % $H_2O$/20 mol % DMSO at $25.0{\pm}0.1^{\circ}C$. The plots of $k_{obsd}$ vs. [piperidine] for the reactions of substrates possessing a strong electron-withdrawing group (EWG) in the leaving group (i.e., 5a-5i) are linear and pass through the origin. In contrast, the plots for the reactions of substrates bearing a weak EWG or no substituent (i.e., 5j or 5k) curve upward, indicating that the electronic nature of the substituent Y in the leaving group governs the reaction mechanism. Thus, it has been suggested that the reactions of 5a-5i proceed through a stepwise mechanism with a zwitterionic tetrahedral intermediate (i.e., $T^{\pm}$) while those of 5j and 5k proceed through a stepwise mechanism with two intermediates (i.e., $T^{\pm}$ and its deprotonated form $T^-$). The slope of the Br${\o}$nsted-type plot for the second-order rate constants (i.e., $k_N$ or $Kk_2$) changes from -0.41 to -1.89 as the leaving-group basicity increases, indicating that a change in the rate-determining step (RDS) occurs. The reactions of 5a-5k with piperidine result in larger $k_1$ values than the corresponding reactions with ethylamine.

• Journal of the Korean Institute of Telematics and Electronics A
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• v.32A no.2
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• pp.89-95
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• 1995

The structure of boron ion-implanted pn junctio in the vacancy-doped p-type HgCdTe was investigated with the differential Hall measurement. The as-implanted junction showed the electron concentration as high as 1${\times}10^{18}/cm^{3}$ and the junction depth of 0.6.mu.m. When the HgCdTe junction was heated in oven, the electron concentration near the junction decreased and the junction depth increased as the annealing temperature and time increased. The junction structure after the thermal annealing was n$^{+}$/n$^{-}$/p. For the 200.deg. C 20min annealed sample, the electron mobility was 10$^{4}cm^{2}/V{\cdot}$s near the surface(n$^{+}$), and was larger thatn 10$^{5}cm^{2}/V{\cdot}$s near the junction(n$^{+}$). The junction formation mechanism is conjectured as follows. When HgCdTe is ion-implanted, the ion energy generates crystal defecis and displaced Hg atoms HgCdTe is ion-implanted, the ion energy generates crystal defecis and displaced Hg atoms near the surface. The displaced Hg vacancies diffuse in easily by the thernal treatment and a fill the Hg vacancies in the p-HgCdTe substrate. With the Hg vacancies filled completely, the GfCdTe substrate becomes n-type because of the residual n-type impurity which was added during the wafer growing. Therefore, the n$^{+}$/n$^{-}$/p regions are formed by crystal defects, residual impurities, and Hg vacancies, respectively.

• Bulletin of the Korean Chemical Society
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• v.24 no.4
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• pp.431-436
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• 2003

Solvolytic rate constants at 25℃ are reported for solvolyses of cinnamyl bromide (1) in binary mixtures of water with acetone, ethanol, methanol, methanol-d, and 2,2,2-trifluoroethanol. Product selectivities are reported for solvolyses of 1 in aqueous ethanol and methanol. Rate ratios in solvents of the same $Y_{Br}$ value and different nucleophilicity provide measures of the minimum extent of nucleophilic solvent assistance (e.g. $[k_{40EW}/k_{97TFE}]$Y = 2.88, EW = ethanol-water). With use of the extended Grunwald-Winstein equation, the l and m values are similar to the values of 0.43 and 0.88 obtained for the solvolyses of 1 using the equation (see below) which includes a parameter (I) for solvation of aromatic rings. The magnitude of l and m values associated with a change of solvent composition predicts the $S_{N1}$ reaction mechanism rather than an $S_{N2}$ channel. Product selectivities (S), defined by S = [ether product]/[alcohol product]×[water]/[alcohol solvent] are related to four rate constants for reactions involving one molecule of solvent as nucleophile and another molecule of solvent as general base catalyst. A linear relationship between 1/S and molar ratio of solvent is derived theoretically and validated experimentally for solvolyses of the above substrates from water up 75% 1/S = $(k_{wa}/k_{aw})$([alcohol solvent]/[water]) + $k_{ww}/k_{aw}$ alcohol-water. The results are best explained by product formation from a “free” carbocation intermediate rather than from a solvent-separated ion pair.

• Transactions of the Korean Society of Pressure Vessels and Piping
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• v.6 no.1
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• pp.50-56
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• 2010

Fatigue has been known as a major degradation mechanism of ASME class 1 components in nuclear power plants. Fatigue damage could be accelerated by combined interaction of several loads and environmental factors. However, the environmental effect is not explicitly addressed in the ASME S-N curve which is based on air at room temperature. Therefore many studies have been performed to understand the environmental effects on fatigue behavior of materials used in nuclear power plants. As a part of efforts, we performed low cycle fatigue tests under various environmental conditions and analyzed the environmental effects on the fatigue life of SA508 Gr. 1a low alloy steel by comparing with higuchi's model. Test results show that the fatigue life depends on water temperature, dissolved oxygen and strain rate. But strain rate over 0.4%/s has little effect on the fatigue life. To find the cause of different fatigue life with ANL's and higuchi's model, another test performed with different heat numbered and heat treated materials of SA508 Gr. 1a. On a metallurgical point of view, the material with bainite microstructure shows much longer fatigue life than that with ferrite/pearlite microstructure. And the characteristics of crack propagation as different microstructure seem to be the main cause of different fatigue life.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.26 no.7
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• pp.1317-1323
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• 2002

This paper describes the comparison of calculated effective stress with experimental one in austenitic heat resistant steels, STS310J1TB and STS310S with and without a small amount of Nb and N. Based on a solute atoms diffusion model, contribution from soluble nitrogen to the high-temperature strength was numerically examined for austenitic heat-resisting Fe-Cr-Ni-N(STS310J1TB) and Fe-Cr-Ni (STS310S) alloys. The solute atmosphere dragging stress of dislocation was calculated in optional dislocation velocity of STS310J1TB and STS310S at $650^{\circ}C$, $675^{\circ}C$ and $700^{\circ}C$. As a result of the numerical calculation, the solute atmosphere dragging stress of STS310J1TB was about 50 times larger than that of STS310S. When the temperature became high, the maximum value of solute atmosphere dragging stress was small and the velocity of moving dislocation was fast. From the relationship between the dislocation rate and the solute atmosphere dragging stress, the relation of both was proportional and the inclination is about 1 in the level with low velocity of moving dislocation. From above results, the mechanism of dislocation movement in STS310J1TB was the solute atmosphere dragging stress. The solute atmosphere dragging stress, which was calculated from the numerical calculation was close to the effect stress in stress relaxation tests.

• Journal of Korean Neurosurgical Society
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• v.60 no.2
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• pp.130-137
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• 2017

Objective : Autophagy is one of the key responses of cells to programmed cell death. Memantine, an approved anti-dementia drug, has an antiproliferative effect on cancer cells but the mechanism is poorly understood. The aim of the present study was to test the possibility of induction of autophagic cell death by memantine in glioma cell lines. Methods : Glioma cell lines (T-98 G and U-251 MG) were used for this study. Results : The antiproliferative effect of memantine was shown on T-98 G cells, which expressed N-methyl-D-aspartate 1 receptor (NMDAR1). Memantine increased the autophagic-related proteins as the conversion ratio of light chain protein 3-II (LC3-II)-/LC3-I and the expression of beclin-1. Memantine also increased formation of autophagic vacuoles observed under a transmission electron microscope. Transfection of small interfering RNA (siRNA) to knock down NMDAR1 in the glioma cells induced resistance to memantine and decreased the LC3-II/LC3-I ratio in T-98 G cells. Conclusion : Our study demonstrates that in glioma cells, memantine inhibits proliferation and induces autophagy mediated by NMDAR1.