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Development and Validation of a Semi-Quantitative Food Frequency Questionnaire to Evaluate Nutritional Status of Korean Elderl (노인의 영양상태 평가를 위한 반정량 식품섭취빈도조사지의 개발 및 타당도 검증)

  • 원혜숙
    • Journal of Nutrition and Health
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    • 제33권3호
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    • pp.314-324
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    • 2000
  • Evaluation of nutritional status of the elderly imposes different problem from the other age groups. It is essential to use right instrument to assess food consumption. In Korea, the food frequency questionnaire has not been applied widely to elderly people. The purpose of this study is to assess the possibility of employing a semi-quantitative food frequency questionnaire(FFQ) for the Korean elderly to estimate nutrient and/or food intakes. In this study the FFQ for the elderly was developed and validated. The subjects were 144 free-living old women aged from 65 to 90. The FFQ was designed with 4 items for cereals and 86 items for other foods and with frequency of 12 intervals. Three portion sizes were given to select : 1/2 of standard amount, standard amount, and 11/2 of standard amount. ach subject was interviewed with newly developed FFQ form and same subjects were also involved to complete 3-day diet record. Nutrient intake was calculated using software program developed by our group. The nutrient intakes by the FFQ was validated by comparing the results with 3-day diet record. The FFQ estimated significantly higher mean intakes of energy, carbohydrate, protein, fats and vitamin C than did the diet reconrds(p<0.05). Pearson's correlation coefficients between two methods ranged from 0.21 for vitamin Q to 0.69 for alcohol(mean r=0.53). From 32% to 42% of the subjects were classified in the same quintile of nutrient intake by two methods, and 63% to 84% were classified in the same or adjacent quintile. On average, only 4% of the subjects were misclassified into extreme quitiles. The results indicate that the FFQ developed for the elderly in this study is useful for classifying individuals by rank and identifying groups at extremes of nutrient intakes.

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Characterization and Identification of Organic Selenium-enriched Bacteria Isolated from Rumen Fluid and Hot Spring Water

  • Dalia, A.M.;Loh, T.C.;Sazili, A.Q.;Jahromi, M.F.;Samsudin, A.A.
    • Microbiology and Biotechnology Letters
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    • 제45권4호
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    • pp.343-353
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    • 2017
  • In the present study, the isolation of selenium (Se)-enriched bacteria from rumen fluid and hot spring water was carried out. Rumen fluid samples were taken from cannulated goats fed a basal diet and the water samples were collected from Selayang hot spring, Selangor-Malaysia. A total number of 140 Se-tolerant isolates were obtained aerobically using an Se-enriched medium and spread plate technique. All the isolates were initially screened for the ability to transform the Se-containing medium to a red-orange culture using a spectrophotometer. Twenty isolates of dark red-orange medium were selected for a screening of the highest Se-containing protein accumulating strains using the dialysis technique and icp.ms to measure the Se content. Four isolates, identified as Enterobacter cloacae (ADS1, ADS7, and ADS11), and Klebsiella pneumoniae (ADS2) from rumen fluid origin, as well as, one isolate from hot spring water (Stenotrophomonas maltophilia (ADS18)), were associated with the highest biomass organic Se-containing protein when grown in a medium enriched with $10{\mu}g/ml$ sodium selenite. In addition, around $50{\mu}g/100{\mu}g$ of the absorbed inorganic Se was accumulated as an organic form. Organic Se-containing protein in all the selected strains showed antioxidant properties in the range of 0.306 to 0.353 Trolox equivalent antioxidant capacity (TEAC) mg/ml. Therefore, these strains may offer a potential source of organic Se due to their Se-tolerant nature and higher biomass organic to inorganic Se ratio.

A Study on the Coupling Performance Improvement of Cylindrical DR Bandpass Filter using Travelling Wave Mode Analysis (진행모드 해석을 이용한 유전체 공진기 대역통과 필터의 결합 특성 개선에 관한 연구)

  • Lee, Won-Hui;Park, Chang-Won;Yang, Jae-Hyuck;Hur, Jung;Lee, J. H.;Lee, Sang-Young
    • Proceedings of the Korea Electromagnetic Engineering Society Conference
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    • 한국전자파학회 2000년도 종합학술발표회 논문집 Vol.10 No.1
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    • pp.125-129
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    • 2000
  • In this paper, We designed and fabricated C-band bandpass filter using dielectric resonators. From waveguide cutoff frequency which applied the region between adjacent dielectric resonators, the height of cavity is determined. The cavity's diameter is determined to the twice of dielectric resonator's diameter considering the conductor loss. The resonant frequency of the DR-cavity is calculated with travelling wave mode analysis. Conventionally, circular cylindrical dielectric resonator is analysed by Cohn's model which use the evanescent mode in the region between dielectric resonator wall and circular cavity wall, which is an approximated method. The external quality factor, Q$_{ex}$ has found with simulation result using Ansoft's Maxwell simulation tool. The designed filter using dielectric resonators with dielectric constant of 45 has the passband center at 5.065GHz. The bandpass filter using dielectric resonators have about 1dB insertion loss. 20MHz bandwidth and more than 30dB attenuation at f$_{0}$$\pm$15MHz.z.z.

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Safety assessment of Generation III nuclear power plant buildings subjected to commercial aircraft crash Part I: FE model establishment and validations

  • Liu, X.;Wu, H.;Qu, Y.G.;Xu, Z.Y.;Sheng, J.H.;Fang, Q.
    • Nuclear Engineering and Technology
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    • 제52권2호
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    • pp.381-396
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    • 2020
  • Investigations of the commercial aircraft impact effect on nuclear island infrastructures have been drawing extensive attention, and this paper aims to perform the safety assessment of Generation III nuclear power plant (NPP) buildings subjected to typical commercial aircrafts crash. At present Part I, finite element (FE) models establishment and validations for both the aircrafts and NPP buildings are performed. (i) Airbus A320 and A380 aircrafts are selected as the representative medium and large commercial aircrafts, and the corresponding fine FE models including the skin, beam, fuel and etc. are established. By comparing the numerically derived impact force time-histories with the existing published literatures, the rationality of aircrafts models is verified. (ii) Fine FE model of the Chinese Zhejiang Sanao NPP buildings is established, including the detailed structures and reinforcing arrangement of both the containment and auxiliary buildings. (iii) By numerically reproducing the existing 1/7.5 scaled aircraft model impact tests on steel plate reinforced concrete (SC) panels and assessing the impact process and velocity time-history of aircraft model, as well as the damage and the maximum deflection of SC panels, the applicability of the existing three concrete constitutive models (i.e., K&C, Winfrith and CSC) are evaluated and the superiority of Winfrith model for SC panels under deformable missile impact is verified. The present work can provide beneficial reference for the integral aircraft crash analyses and structural damage assessment in the following two parts of this paper.

Genomic Screening for Targets Regulated by Berberine in Breast Cancer Cells

  • Wen, Chun-Jie;Wu, Lan-Xiang;Fu, Li-Juan;Yu, Jing;Zhang, Yi-Wen;Zhang, Xue;Zhou, Hong-Hao
    • Asian Pacific Journal of Cancer Prevention
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    • 제14권10호
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    • pp.6089-6094
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    • 2013
  • Berberine, a common isoquinoline alkaloid, has been shown to possess anti-cancer activities. However, the underlying molecular mechanisms are still not completely understood. In the current study, we investigated the effects of berberine on cell growth, colony formation, cell cycle distribution, and whether it improved the anticancer efficiency of cisplatin and doxorubicin in human breast cancer estrogen receptor positive (ER+) MCF-7 cells and estrogen receptor negative (ER-) MDA-MB-231 cells. Notably, berberine treatment significantly inhibited cell growth and colony formation in the two cell lines, berberine in combination with cisplatin exerting synergistic growth inhibitory effects. Accompanied by decreased growth, berberine induced G1 phase arrest in MCF-7 but not MDA-MB-231 cells. To provide a more detailed understanding of the mechanisms of action of berberine, we performed genome-wide expression profiling of berberine-treated cells using cDNA microarrays. This revealed that there were 3,397 and 2,706 genes regulated by berberine in MCF-7 and MDA-MB-231 cells, respectively. Fene oncology (GO) analysis identified that many of the target genes were involved in regulation of the cell cycle, cell migration, apoptosis, and drug responses. To confirm the microarray data, qPCR analysis was conducted for 10 selected genes based on previously reported associations with breast cancer and GO analysis. In conclusion, berberine exhibits inhibitory effects on breast cancer cells proliferation, which is likely mediated by alteration of gene expression profiles.

Keeping house: evaluation of housekeeping genes for real-time PCR in the red alga, Bostrychia moritziana (Florideophyceae)

  • Shim, Junbo;Shim, Eunyoung;Kim, Gwang Hoon;Han, Jong Won;Zuccarello, Giuseppe C.
    • ALGAE
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    • 제31권2호
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    • pp.167-174
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    • 2016
  • Biological response of cells to variable conditions should affect the expression level of certain genes. Quantification of these changes in target genes needs stable internal controls. Real-time quantitative polymerase chain reaction (PCR) has traditionally used reference or ‘housekeeping’ genes, that are considered to maintain equal expression in different conditions, to evaluate changes in target genes between samples and experimental conditions. Recent studies showed that some housekeeping genes may vary considerably in certain biological samples. This has not been evaluated in red algae. In order to identify the optimal internal controls for real-time PCR, we studied the expression of eleven commonly used housekeeping genes; elongation factor 1-alpha, glyceraldehyde-3-phosphate dehydrogenase, β-actin, polyubiquitin, 30S ribosomal gene, 60S ribosomal gene, beta-tubulin, alpha-tubulin, translation initiation factor, ubiquitin-conjugating enzyme, and isocitrate dehydrogenase in different life-history stages of Bostrychia moritziana. Our results suggest that glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and 30S ribosomal gene, have the most stable gene expression levels between the different life history stages (male, female, carposporophyte, and tetrasporophyte), while the other genes are not satisfactory as internal controls. These results suggest that the combinations of GAPDH and 30S would be useful as internal controls to assess expression level changes in genes that may control different physiological processes in this organism or that may change in different life history stages. These results may also be useful in other red algal systems.

Bleeding Efficiency and Meat Oxidative Stability and Microbiological Quality of New Zealand White Rabbits Subjected to Halal Slaughter without Stunning and Gas Stun-killing

  • Nakyinsige, K.;Fatimah, A.B.;Aghwan, Z.A.;Zulkifli, I.;Goh, Y.M.;Sazili, A.Q.
    • Asian-Australasian Journal of Animal Sciences
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    • 제27권3호
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    • pp.406-413
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    • 2014
  • A study was conducted to compare the effect of halal slaughter without stunning and gas stun killing followed by bleeding on residual blood content and storage stability of rabbit meat. Eighty male New Zealand white rabbits were divided into two groups of 40 animals each and subjected to either halal slaughter without stunning (HS) or gas stun-kill (GK). The volume of blood lost during exsanguination was measured. Residual blood was further quantified by determination of haemoglobin content in Longissimus lumborum (LL) muscle. Storage stability of the meat was evaluated by microbiological analysis and measuring lipid oxidation in terms of thiobarbituric acid reactive substances (TBARS). HS resulted in significantly higher blood loss than GK. HS had significantly lower residual haemoglobin in LL muscle compared to GK. Slaughter method had no effect on rabbit meat lipid oxidation at 0, 1, and 3 d postmortem. However, at 5 and 8 days of storage at $4^{\circ}C$, significant differences (p<0.05) were found, with meat from the GK group exhibiting significantly higher levels of MDA than that from HS. At day 3, greater growth of Pseudomonas aeroginosa and E. coli were observed in the GK group (p<0.05) with B. thermosphacta and total aerobic counts remained unaffected by slaughter method. At days 5 and 7 postmortem, bacterial counts for all tested microbes were affected by slaughter method, with GK exhibiting significantly higher growth than HS. It can be concluded that slaughter method can affect keeping quality of rabbit meat, and HS may be a favourable option compared to GK due to high bleed out.

Comparisons of Quality of Life, Sexual Function, and Depression in Sexually Active or Inactive Groups of Women with Mastectomy (유방암 생존자의 삶의 질, 성기능 및 우울: 성생활 집단과 비성생활 집단 간의 비교)

  • Kim, Hae-Young;So, Hyang-Sook;Chae, Myeong-Jeong;Kim, Kyoung-Mi
    • Asian Oncology Nursing
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    • 제8권2호
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    • pp.77-85
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    • 2008
  • Purpose: This study was to compare quality of life, sexual function, and depression between a group with sexually active women and the other with sexually inactive women who undertaken mastectomy. Methods: Participants were 106 breast cancer patients who had spouse and participated in self-help group in Gwangju, Korea. The questionnaires were consisted of Quality of Life Index-Cancer Version (Q.L.I.-C), self-rating depression scale, and Female Sexual Function Index (FSFI). Data were analyzed using the SPSS Win 14.0 K+ for descriptive statistics, t-test, ANCOVA, and MANOVA. Results: There were no homogeneity at age, job, educational years between sexually active vs. inactive groups. In sexually active group, mean age was 46.1, having job 34.5%, bachelor's degree 38.2%, and pre-menopause 79.2%; for the inactive group 49.6, 10.4%, 12.5%, and 52.7% seperately. There were significant differences in quality of life, sexual function, and depression between two groups when four covariates were controlled. The quality of life and sexual function in sexually active group were significantly higher than inactive group(p<0.001). The depression in sexually active group was significantly lower than inactive group(p<0.001). Conclusion: This study indicates that nurses are needed to counsel and intervene psychosexual approach especially sexually inactive women with breast cancer during the recovery period.

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Effect of Hybridization on Carcass Traits and Meat Quality of Erlang Mountainous Chickens

  • Yin, H.D.;Gilbert, E.R.;Chen, S.Y.;Wang, Y.;Zhang, Z.C.;Zhao, X.L.;Zhang, Yao;Zhu, Q.
    • Asian-Australasian Journal of Animal Sciences
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    • 제26권10호
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    • pp.1504-1510
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    • 2013
  • Native chickens hold a significant share of the market in China. In response to the huge demand from the market, the productivity of Chinese native chickens needs to be improved. Cross breeding is an effective method to increase productivity, although it might affect meat quality. In this study, two pure lines (SD02 and SD03) of Erlang mountainous chickens were hybridized with a yellow feather and faster growing line (SD01). The effect of hybridization on carcass and meat quality (physiochemical and textural traits) was measured in the $F_1$ population at d 91 of age. The hybrids exhibited higher body weight and dressed weight, and amount of semi-eviscerated, eviscerated, breast muscle and abdominal fat (p<0.05). Abdominal fat yield also increased (p<0.05) compared to the offspring of the two pure-lines. Meanwhile, there was no significant difference in meat quality traits except for the myofiber diameter and density and the shear force of the breast muscle. Overall, the offspring of cross-lines were similar to pure lines in meat color, pH value, inosinic acid, crude protein, crude fat, dry matter, moisture content and amino acid composition in the breast muscle. These results suggest that productivity can be improved via cross-breeding while maintaining meat quality of the Erlang mountainous chicken.

Influence of Composition on Soft Magnetic Properties of As-Deposited Fe-Sm-O Thin Films (조성변화에 따른 Fe-Sm-O계 박막의 연자기적 성질)

  • Yoon, T.S.;Cho, W.S.;Koo, E.S.;Li, Ying;Park, J.B.;Kim, C.O.
    • Korean Journal of Materials Research
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    • 제11권1호
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    • pp.39-43
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    • 2001
  • Nanocrystalline Fe-Sm-O thin films were prepared by RF magnetron reactive sputtering method in $Ar+O_2$mixed atmosphere with the $O_2$content of 5%. The compositions of the thin films were changed by changing the number of $Sm_2O_3$ chips. The best soft magnetic properties of the thin film with the composition of $Fe_{83.4}Sm_{3.4}O_{13.2}$ were saturation flux density of 18 kG, coercivity of 0.82 Oe and effective permeability about 2,600 at 0.5~100 MHz, respectively. The electrical resistivity of Fe-Sm-O thin films was increased with increasing the amount of Sm and O elements which combined each other, the electrical resistivity of$Fe_{83.4}Sm_{3.4}O_{13.2}$ thin film was $130{\mu}{\Omega}cm$. In case of the small amount of Sm and O elements, the microstructures of Fe-Sm-O thin films showed a precipitated phase of $Sm_2O_3$ on the ${\alpha}-Fe$ phase. With the increase of the amount of Sm and O elements, the microstructures of the Fe- Sm-O thin films were changed into a mixed structure of ${\alpha}-Fe$ crystal-phase and Sm-oxide amorphous phase. The Fe-Sm-O thin films with Fe content in the range of 72~94 at% exhibited the quality factor (Q = $\mu$′/$\mu$") of 7~75 up to 50 MHz.

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