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The Effect of Energy Supplementation on Intake and Utilisation Efficiency of Urea-treated Low-quality Roughage in Sheep I. Rumen Digestion and Feed Intake

  • Migwi, P.K.;Godwin, I.;Nolan, J.V.;Kahn, L.P.
    • Asian-Australasian Journal of Animal Sciences
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    • 제24권5호
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    • pp.623-635
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    • 2011
  • Inefficient rumen microbial fermentation is a major factor limiting intake of low quality roughage in ruminants. In this study, the effect of energy supplementation on rumen microbial fermentation, absorption of balanced digestion products and voluntary feed intake in sheep was investigated. A basal diet of a urea-treated mixture of wheaten chaff and barley straw (3:1 DM) containing 22.2 g N/kg DM was used. Four Merino-cross wethers weighing $45{\pm}4.38\;kg$ and fitted with permanent rumen and abomasal cannulae were allocated to four treatments in a $4{\times}4$ Latin square design. The dietary treatments were basal diet ($E_0$), or basal diet supplemented with sucrose (112.5 g/d) administered to the animals intra-ruminally ($E_R$), abomasally ($E_A$), or through both routes (50:50) ($E_{RA}$). Feed intake (basal and dietary) was increased (p<0.05) by sucrose supplementation through the rumen ($E_R$) or abomasum ($E_A$). However, there was no difference (p>0.05) in intake between animals on the control diet and those supplemented with sucrose through both intraruminal and abomasal routes ($E_{RA}$). The digestibility of DM and OM was highest in $E_R$ and $E_A$ supplemented animals. Although the rumen pH was reduced (p<0.001) in animals supplemented with sucrose entirely intra-ruminally ($E_R$), the in sacco degradation of barley straw in the rumen was not adversely affected (p>0.05). Intra-ruminal sucrose supplementation resulted in a higher concentration of total VFA, acetate and butyrate, while the pattern of fermentation showed a higher propionate: acetate ratio. Intra-ruminal supplementation also increased (p<0.05) the glucogenic potential (G/E) of the absorbed VFA. However, there was no difference (p>0.05) in microbial protein production between the four dietary treatments. Protozoa numbers were increased (p<0.05) by intra-ruminal supplementation of sucrose.

개에서 단일 통로 유연 내시경 담낭 절제술 : 유용성 연구 (Single Incision Flexible Endoscopic Cholecystectomy in Dogs : Feasibility Study)

  • 이소연;신범준;정성목
    • 한국임상수의학회지
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    • 제28권6호
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    • pp.555-561
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    • 2011
  • 본 연구에서는 중 소형견에서 1개의 복강경 통로를 통한 내시경적 담낭 제거술을 실시함으로써, 개에서 최소 침습적 수술로 담낭 절제술을 실시할 수 있는 방법을 확립하기 위하여 실시하였다. 실험 동물로 총 3마리의 수컷 비글견 ($10.3{\pm}0.62$ kg)을 이용하였으며 배꼽 주위에 하나의 절개창을 만든 후 단일 통로 내시경 시스템을 장착하였다. 이 시스템을 통해 내시경과 Maryland 복강경용 겸자로 담낭을 견인, 제거하였다. 3마리 모두 성공적으로 담낭 절제술을 실시하였으며, 수술 후 감염과 같은 합병증은 발생하지 않았다. 또한 술전, 술후를 비교한 혈액, 혈청 검사상에서도 특이적인 소견은 관찰되지 않았다. 술후 7일 후에 이루어진 부검 소견에서도 출혈 및 담즙의 유출 및 복막염 등과 같은 부작용은 관찰되지 않았다. 따라서 본 연구를 통해, 단일 통로를 이용한 내시경 유도하 담낭 절제술은 중소형견에서 실시할 수 있는 복강 수술 방법 중 최소한의 절개를 통해 비침습적으로 이루어질 수 있는 유용한 수술 방법이라 할 수 있다.

Evaluation of Biochemical Recurrence-free Survival after Radical Prostatectomy by Cancer of the Prostate Risk Assessment Post-Surgical (CAPRA-S) Score

  • Aktas, Binhan Kagan;Ozden, Cuneyt;Bulut, Suleyman;Tagci, Suleyman;Erbay, Guven;Gokkaya, Cevdet Serkan;Baykam, Mehmet Murat;Memis, Ali
    • Asian Pacific Journal of Cancer Prevention
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    • 제16권6호
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    • pp.2527-2530
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    • 2015
  • Background: The cancer of the prostate risk assessment (CAPRA) score has been defined to predict prostate cancer recurrence based on the pre-clinical data, then pathological data have also been incorporated. Thus, CAPRA post-surgical (CAPRA-S) score has been developed based on six criteria (prostate specific antigen (PSA) at diagnosis, pathological Gleason score, and information on surgical margin, seminal vesicle invasion, extracapsular extension and lymph node involvement) for the prediction of post-surgical recurrences. In the present study, biochemical recurrence (BCR)-free probabilities after open retropubic radical prostatectomy (RP) were evaluated by the CAPRA-S scoring system and its three-risk level model. Materials and Methods: CAPRA-S scores (0-12) of our 240 radical prostatectomies performed between January 2000-May 2011 were calculated. Patients were distributed into CAPRA-S score groups and also into three-risk groups as low, intermediate and high. BCR-free probabilities were assessed and compared using Kaplan-Meier analysis and Cox proportional hazards regression. Ability of CAPRA-S in BCR detection was evaluated by concordance index (c-index). Results: BCR was present in 41 of total 240 patients (17.1%) and the mean follow-up time was $51.7{\pm}33.0$ months. Mean BCR-free survival time was 98.3 months (95% CI: 92.3-104.2). Of the patients in low, intermediate and high risk groups, 5.4%, 22.0% and 58.8% had BCR, respectively and the difference among the three groups was significant (P = 0.0001). C-indices of CAPRA-S score and three-risk groups for detecting BCR-free probabilities in 5-yr were 0.87 and 0.81, respectively. Conclusions: Both CAPRA-S score and its three-risk level model well predicted BCR after RP with high c-index levels in our center. Therefore, it is a clinically reliable post-operative risk stratifier and disease recurrence predictor for prostate cancer.

Prevalence of Colorectal Polyps in a Group of Subjects at Average-risk of Colorectal Cancer Undergoing Colonoscopic Screening in Tehran, Iran between 2008 and 2013

  • Sohrabi, Masoudreza;Zamani, Farhad;Ajdarkosh, Hossien;Rakhshani, Naser;Ameli, Mitra;Mohamadnejad, Mehdi;Kabir, Ali;Hemmasi, Gholamreza;Khonsari, Mahmoudreza;Motamed, Nima
    • Asian Pacific Journal of Cancer Prevention
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    • 제15권22호
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    • pp.9773-9779
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    • 2014
  • Background: Colorectal cancer (CRC) is one of the prime causes of mortality around the globe, with a significantly rising incidence in the Middle East region in recent decades. Since detection of CRC in the early stages is an important issue, and also since to date there are no comprehensive epidemiologic studies depicting the Middle East region with special attention to the average risk group, further investigation is of significant necessity in this regard. Aim: Our aim was to investigate the prevalence of preneoplastic and neoplastic lesions of the colon in an average risk population. Materials and Methods: A total of 1,208 eligible asymptomatic, average- risk adults older than 40 years of age, referred to Firuzgar Hospotal in the years 2008-2012, were enrolled. They underwent colonoscopy screening and all polypoid lesions were removed and examined by an expert gastrointestinal pathologist. The lesions were classified by size, location, numbers and pathologic findings. Size of lesions was measured objectively by endoscopists. Results: The mean age of participants was $56.5{\pm}9.59$ and 51.6% were male. The overall polyp detection rate was 199/1208 (16.5 %), 26 subjects having non-neoplastic polyps, including hyperplastic lesions, and 173/1208 (14.3%) having neoplastic polyps, of which 26 (2.15%) were advanced neoplasms. The prevalence of colorectal neoplasia was more common among the 50-59 age group. Advanced adenoma was more frequent among the 60-69 age group. The majority of adenomas were detected in the distal colon, but a quarter of advanced adenomas were found in the proximal colon; advance age and male gender was associated with the presence of adenoma. Conclusions: It seems that CRC screening among average-risk population might be recommended in countries such as Iran. However, sigmioidoscopy alone would miss many colorectal adenomas. Furthermore, the 50-59 age group could be considered as an appropriate target population for this purpose in Iran.

족삼리(足三里)와 위유(胃兪)의 경혈배합(經穴配合)과 병용자극(竝用刺戟)이 정상(正常) 백서(白鼠) 위기능(胃機能)에 미치는 영향(影響) (Effects of Combined Electro-Acupuncture at Zusanli(ST36) and Moxibustion at Weishu(BL21) on the Serum Gastrin Level in Rats)

  • 윤민영;김경식;황우준;이건목;이옥자;김홍훈;윤정안;유윤조;박종운;조남근
    • Journal of Acupuncture Research
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    • 제21권5호
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    • pp.45-58
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    • 2004
  • It is well known that both of acupuncture and moxibustion are effective for treatment of human disease. For years, they have been utilizing a electro-acupuncture (EA) and moxibustion in attempt to manage gastric symptom. However, the data of combined EA and moxibustion on clinical effectiveness is conflicting and the action of combineAd acupoints is not well known. BL21 and ST36 which belong to bladder Meridian and stomach meridian are acupuncture points frequently used for healing gastrointestinal disease in oriental medicine. The present study was conducted to see the effects produced by combined electro-acupuncture(EA) and moxibustion using BL21 and ST36 on serum gastrin level in rats. In experiment, male Spraque-Dawley rats(body wt 140-160g) were selected. EA (2 Hz, intensity ; 10 times muscle twitch threshold) was applied for 30 minutes to ST36 under halothane anesthesia. Moxibustion($1.6{\pm}0.3 mg$, 5 times of a day) was applied during 3 days to BL21 under halothane anesthesia. Synergy effects of combined EA and moxibustion might be in according to stimulated method and used acupoints. The change of serum gastrin levels produced by combined EA and moxibustion is shown after vagotomy. These data suggested that many factors, such as the selection of acupoints, the stimulated EA methods, are capable of affecting combined EA and moxibustion effects and those effects may be not related with nervous system.

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Effect of Ground Corn Cob Replacement for Cassava Chip on Feed Intake, Rumen Fermentation and Urinary Derivatives in Swamp Buffaloes

  • Wanapat, M.;Pilajun, R.;Kang, S.;Setyaningsih, K.;Setyawan, A.R.
    • Asian-Australasian Journal of Animal Sciences
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    • 제25권8호
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    • pp.1124-1131
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    • 2012
  • Four Thai - rumen fistulated male swamp buffaloes (Bubalus bubalis), about four years old with $400{\pm}20kg$ liveweight, were randomly assigned according to a $4{\times}4$ Latin square design to receive dietary treatments. The treatments were: ground corn cob (GCC) replacement for cassava chip (CC) in concentrate at 0% (T1); GCC replacement at 33% (T2); GCC replacement at 67% (T3); and GCC replacement at 100% (T4), respectively. During the experiment, concentrate was offered at 0.5% BW while 5% urea-treated rice straw was given at ad libitum. The result revealed that there was no effect of GCC replacement on DMI among treatments. In addition, digestibilities of DM, OM and CP were not different while aNDF linearly increased with an increasing level of GCC replacement. However, GCC replacement did not affect rumen fermentation such as ruminal pH, $NH_3$-N and VFA concentration; except C3 proportion which was the highest at 33% replacement while the lowest was at 100% replacement. All replacements of GCC resulted in similar protozoal and bacterial populations and microbial protein synthesis (MPS). Purine derivatives (PD) concentration in urine and PD to creatinine (PDC) index were varied with time of urination and among treatments at 0 to 8 and 8 to 16 h post feeding and higher values were shown among the GCC replacement groups. However at 16 to 24 h-post feeding, it was untraceable. In addition, creatinine concentration was similar among all treatments at every sampling time. Based on the above results, GCC can be used as an energy source for swamp buffalo fed with rice straw. Spot sampling of urine can be used for purine derivatives determination.

정신분열병의 결핍증후군과 비결핍증후군에서 QEEG와 sLORETA를 이용한 비교연구 (Comparing Quantitative EEG and Low Resolution Electromagnetic Tomography Imaging between Deficit Syndrome and Non-Deficit Syndrome of Schizophrenia)

  • 이상은;임선진;이미경;이재원;한규희;이종일;심민영;윤해주;신병학
    • 수면정신생리
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    • 제17권2호
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    • pp.91-99
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    • 2010
  • 목 적: 본 연구는 정신분열병의 결핍증후군과 비결핍증후군이 다른 생물학적 동등성을 가진 독립된 질환일 수 있다는 가설 아래 quantitative EEG와 standardized LORETA (sLORETA)를 이용한 전기생리학적인 방법을 통하여 생물학적 병인을 파악하고자 시도되었다. 방 법: 정신분열병 환자를 대상으로 42명의 뇌파를 비교 분석하였으며 그 중 결핍증후군 환자군은 남자 10명과 여자 11명이었고 비결핍증후군 환자군은 남자 12명, 여자 9명이었다. 주파수 대역은 delta(1.5~4 Hz), theta(4~8 Hz), alpha(8~12 Hz), low beta(12~15 Hz), high beta(15~30 Hz)의 5가지로 분할하였고 EEG LAB을 이용한 파워스펙트럼 분석 및 standardized sLORETA software package를 이용하여 신호원을 국소화 하였다. 결 과: 파워 스펙트럼 분석에서 결핍증후군 집단은 비결핍증후군과 비교하였을 때 전두엽, 두정엽 및 측두엽 영역에서 delta파와 theta파의 유의한 활성도 증가를 보였으며 뇌파 스펙트럼은 간편 정신상태 평정 척도 중 철퇴/지연과 적대/의심 항목의 임상적인 특징과 유의한 상관관계를 보였다. sLORETA분석 결과에서는 배측 전대상피질에서 결핍증후군에서 유의하게 delta파의 활성도가 증가되었다. 결 론: 결핍증후군은 비결핍증후군과는 연관된 뇌 영역이 다를 수 있으며 특히 전두엽 영역의 신경회로 이상이 일차적 음성증상에 영향을 줄 것으로 생각된다.

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지골피와 생강 정제 추출물이 고지방식이 흰쥐의 체내 지질과 혈청 Cytokine 수준에 미치는 영향 (Effects of the purified extracts from Lycii Cortex Radicis and ginger on lipid statusand serum cytokine levels in rats fed high fat diet)

  • 박은정;최상원;조성희
    • Journal of Nutrition and Health
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    • 제45권5호
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    • pp.411-419
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    • 2012
  • The present study was to investigate the effects of Lycii Cortex Radicis (LCR), the root bark of lycium (Lycium chenese Miller) and ginger (Gin) on body lipid status and serum levels of cytokines. Sprague-Dawley (SD) male rats weighing $193.6{\pm}16.8g$ were divided into five groups, including one low fat (LF) and four high fat groups, i.e. HF-Control, HF-LCR, HF-Gin and HF-LCR + Gin groups. Diets for HF-LCR, HF-Gin and HF-LCR + Gin groups contained purified extracts having 0.2 g LCR tyramine, ginerol and 0.1 g tyramine plus 0.02 g gingerol per kg, respectively. Compared with those of the HF-Control total serum cholesterol level decreased, and HDL-cholesterol level increased in the HF-LCR group and serum triglyceride levels decreased in the three experimental groups fed the purified extracts. Liver cholesterol level was lower in the HF-LCR group than the HF-Control group, but triglyceride levels, which were increased by high fat diets were not changed by significantly by LCR or ginger extracts. Fecal lipid excretion was higher in the HF-LCR and HF-Gin groups, but cholesterol excretion was lower in the HF-Gin group than in the HF-Control group. The activities of liver cytosolic glucose-6-phosphate dehydrogenase and malic enzyme were lower in the HF-LCR + Gin group than in the HF-Control group. Serum adiponectin levels did not differ among the five groups, while leptin level was lower in the HF-Gin group and C-reactive protein levels were lower in the HF-Gin and the HF-LCR + Gin groups than in the HF-Control group. It is concluded that LCR can be utilized as an ingredient for lipid-lowering functional foods in the form of purified extract and addition of small amount of ginger extract would be useful for reducing one of the inflammatory cytokines to help prevent atherosclerosis.

죽초액을 급여한 돼지고기의 저장 중 육질특성 변화 (Changes in Meat Quality Characteristics on Refrigerated Pork Loin Fed with Supplemental Bamboo Vinegar)

  • 국길;김광현
    • Journal of Animal Science and Technology
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    • 제45권2호
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    • pp.265-272
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    • 2003
  • 본 연구는 평균체중 60.0$\pm$1.5kg인 육성비육돈 30두를 대조구, 죽초액 2% 급여구 및 죽초액 4% 급여구의 3개 처리구로 10두씩 배치하여 4개월간 사양시험을 실시한 후 도축하여 등심육을 시료로 채취하여 저장 중 육질특성 변화를 알아보고자 실시하였다. pH는 저장 3일 이후 죽초액 2%와 4% 급여구가 대조구에 비해 유의적(P<0.05)으로 감소함을 나타냈다. 명도(L)는 저장 1일과 3일에 2%와 4% 급여구가 대조구에 비해 유의적(P<0.05)으로 높게 나타났다. 적색도는 저장 1일과 3일에 죽초액 4% 급여구가 대조구에 비해 유의적(P<0.05)으로 낮게 나타났다. 황색도는 저장기간 동안 죽초액 2%와 4% 급여구가 대조구에 비해 유의적(P<0.05)으로 높게 나타났다. 가열감량은 저장 3일 이후 죽초액 2%와 4%급여구가 대조구에 비해 유의적(P<0.05)으로 높게 나타났다. 전단력은 저장 1일과 3일에 죽초액 2%와 4% 급여구가 대조구에 비해 유의적(P<0.05)으로 낮게 나타나 연도 개선효과를 보였다. 지방산패도는 저장 6일과 9일에 죽초액 2%와 4% 급여구가 유의적(P<0.05)으로 감소하였다. 총균수는 죽초액 2%와 4% 급여구가 대조구에 유의적(p<0.05)으로 낮게 나타났다. 냄새는 저장 1일과 3일에 죽초액 2%와 4% 급여구가 대조구에 비해 유의적(P<0.05)으로 높은 점수를 받아 돼지 특유의 이취가 감소하는 효과를 나타냈다. 외관은 저장 1일에 죽초액 2%와 4% 급여구가 대조구에 비해 유의적(P<0.05)으로 증가함을 나타냈다. 맛은 저장 1일과 3일, 6일에 죽초액 2%와 4% 급여구가 대조구에 비해 유의적(P<0.05)으로 높게 나타났다. 이상의 결과를 종합해보면 죽초액 2%와 4% 급여된 돼지고기는 육질특성과 저장안정성이 개선된 것으로 사료된다.

Nutrient Utilization, Body Composition and Lactation Performance of First Lactation Bali Cows (Bos sondaicus) on Grass-Legume Based Diets

  • Sukarini, I.A.M.;Sastradipradja, D.;Sutardi, T.;Mahardika, IG.;Budiarta, IG.A.
    • Asian-Australasian Journal of Animal Sciences
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    • 제13권12호
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    • pp.1681-1690
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    • 2000
  • A study on energy and protein utilization, and milk production of Bali cows on grass-legume diets was carried out using 12 first lactation cows (initial BW $263.79{\pm}21.66kg$) during a period of 16 weeks starting immediately post calving. The animals were randomly allotted into 4 dietary treatment groups R1, R2, R3 and R4, receiving from the last 2 months of pregnancy onwards, graded improved rations based on a mixture of locally available grass and legume feed ad libitum. R1 contained on a DM basis 70% elephant grass (PP, Penisetum purpureum) plus 30% Gliricidia sepia leaves (GS), R2 was 30% PP plus 55% GS supplemented with 15% Hibiscus tilliactus leaves (HT, defaunating effect), R3 and R4 were 22.5% PP+41.25% GS+11.25% HT+25% concentrate, where R3 was not and R4 supplemented with zinc di-acetate. TDN, CP and zinc contents of the diets were 58.2%, 12.05% and 18.3 mg/kg respectively for R1, 65.05%, 16.9% and 25.6 mg/kg respectively for R2, 66.03%, 16.71% and 29.02 mg/kg respectively for R3 and 66.03%, 16.71% and 60.47 mg/kg respectively for R4. Milk production and body weight were monitored throughout the experimental period. In vivo body composition by the urea space technique validated by the body density method and supported by carcass data was estimated at the start and termination of the experiment. Nutrient balance and rumen performance characteristics were measured during a balance trial of 7 days during the 3rd and 4th week of the lactation period. Results indicated that quality of ration caused improvement of ruminal total VFA concentration, increments being 52 to 65% for R2, R3 and R4 above R1, with increments of acetate being less (31 to 48%) and propionate being proportionally more in comparison to total VFA increments. Similarly, ammonia concentrations increased to 5.24 to 7.07 mM, equivalent to 7.34 to 9.90 mg $NH_3-N/100ml$ rumen fluid. Results also indicated that feed quality did not affect DE and ME intakes, and heat production (HP), but increased GE, UE, energy in milk and total retained energy (RE total) in body tissues and milk. Intake-, digestible- and catabolized-protein, and retained-protein in body tissues and milk (Rprot) were all elevated increasing the quality of ration. Similar results were obtained for milk yield and components with mean values reaching 2.085 kg/d (R4) versus 0.92 kg/d (R1) for milk yield, and 170.22 g/d (R4) vs 71.69 g/d (R1), 105.74 g/d (R4) vs 45.35 g/d (R1), 101.34 g/d (R4) vs 46.36 g/d (R1) for milk-fat, -protein, and -lactose, respectively. Relatively high yields of milk production was maintained longer for R4 as compared to the other treatment groups. There were no significant effects on body mass and components due to lactation. From the relationship $RE_{total}$ (MJ/d)=12.79-0.373 ME (MJ/d); (r=0.73), it was found that $ME_{m}=0.53MJ/kgW^{0.75}.d$. Requirement of energy to support the production of milk, ranging from 0.5 to 3.0 kg/d, follows the equation: Milk Prod. ($Q_{mp}$, kg/d)=[-2.48+4.31 ME($MJ/kg^{0.75}.d$)]; (r=0.6) or $Q_{mp}$=-3.4+[0.08($ME-RE_{body\;tissue}$)]MJ/d]; (r=0.94). The requirement for protein intake for maintenance ($IP_m$) equals $6.19 g/kg^{0.75}.d$ derived from the relationship RP=-47.4+0.12 IP; (r=0.74, n=9). Equation for protein requirement for lactation is $Q_{nl}$=[($Q_{mp}$)(% protein in milk)($I_{mp}$)]/100, where $Q_{nl}$ is g protein required for lactation, $Q_{mp}$ is daily milk yield, Bali cow's milk-protein content av. 5.04%, and $I_{mp}$ is metabolic increment for milk production ($ME_{lakt}/ME_{m}=1.46$).